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The map label for this gene is rnhB

Identifier: 15645936

GI number: 15645936

Start: 1383299

End: 1383928

Strand: Reverse

Name: rnhB

Synonym: HP1323

Alternate gene names: 15645936

Gene position: 1383928-1383299 (Counterclockwise)

Preceding gene: 15645937

Following gene: 15645933

Centisome position: 82.98

GC content: 42.54

Gene sequence:

>630_bases
TTGGGTTGCGTATCAATGACTCTAGGCATTGATGAAGCGGGTAGGGGGTGTTTGGCCGGTTCGCTTTTTGTGGCTGGGGT
GGCGTGTAATGAAAAAACAGCCTTAGAATTTCTAAAAATGGGTTTAAAAGACAGCAAGAAGCTCAGCCTAAAAAAGCGCT
TTTTCTTAGAATATAAGATCAAAACGCATGGTGAGGTGGGGTTTTTCGTGGTTAAAAAAAGCGCAAATGAAATTGATAGC
TTGGGCTTAGGGGCGTGTTTGAAACTCGCTGTGCAAGAAATTTTAGAAAATGGTTGCTCTTTAGTTGATGAAATAAAAAT
AGACGGCAACACGGCGTTTGGCTTGAACAAACGCTACCCCCATATACAAACCATCATCAAGGGCGATGAAACAATCGCTC
AAATCGCTATGGCGTCTGTTTTGGCGAAAGCTTTTAAGGACAGAGAAATGCTAGAGTTGCACGCTTTGTTTAAGGAATAC
GGCTGGGATAAGAATTGCGGGTATGGGACTAAACAACATATAGAAGCGATCATTAAGCTAGGGGCTACGCCTTTTCATCG
GCATAGCTTCACGCTTAAAAACCGCATCTTAAATCCCAAACTCTTAGAGGTGGAACAACGCCTTATTTAA

Upstream 100 bases:

>100_bases
AAGCGTGAAAAACTTTGAAAAAGAATTAACAGAGTTTATTAAGAGACTAAGAGAAAACAGGATAACGCATGAAACATGTG
AGTAGGGATTTTGATACCGG

Downstream 100 bases:

>100_bases
AAGGGCGCTGAGATGGGTAGCGCTCGCTGAAGAAAGGTCGATGCGTTTGGCTTTAAAGTAAGCGATCAAGTCTTCAGGCT
GAATGAAAGGGAATTGCACC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MGCVSMTLGIDEAGRGCLAGSLFVAGVACNEKTALEFLKMGLKDSKKLSLKKRFFLEYKIKTHGEVGFFVVKKSANEIDS
LGLGACLKLAVQEILENGCSLVDEIKIDGNTAFGLNKRYPHIQTIIKGDETIAQIAMASVLAKAFKDREMLELHALFKEY
GWDKNCGYGTKQHIEAIIKLGATPFHRHSFTLKNRILNPKLLEVEQRLI

Sequences:

>Translated_209_residues
MGCVSMTLGIDEAGRGCLAGSLFVAGVACNEKTALEFLKMGLKDSKKLSLKKRFFLEYKIKTHGEVGFFVVKKSANEIDS
LGLGACLKLAVQEILENGCSLVDEIKIDGNTAFGLNKRYPHIQTIIKGDETIAQIAMASVLAKAFKDREMLELHALFKEY
GWDKNCGYGTKQHIEAIIKLGATPFHRHSFTLKNRILNPKLLEVEQRLI
>Mature_208_residues
GCVSMTLGIDEAGRGCLAGSLFVAGVACNEKTALEFLKMGLKDSKKLSLKKRFFLEYKIKTHGEVGFFVVKKSANEIDSL
GLGACLKLAVQEILENGCSLVDEIKIDGNTAFGLNKRYPHIQTIIKGDETIAQIAMASVLAKAFKDREMLELHALFKEYG
WDKNCGYGTKQHIEAIIKLGATPFHRHSFTLKNRILNPKLLEVEQRLI

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=192, Percent_Identity=33.8541666666667, Blast_Score=100, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 23177; Mature: 23045

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCVSMTLGIDEAGRGCLAGSLFVAGVACNEKTALEFLKMGLKDSKKLSLKKRFFLEYKI
CCCEEEEECCCCCCCCHHHHHHHHEEECCCHHHHHHHHHHCCCCCHHHHHHHHEEEEEEE
KTHGEVGFFVVKKSANEIDSLGLGACLKLAVQEILENGCSLVDEIKIDGNTAFGLNKRYP
ECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHEEECCCEEECCCCCCC
HIQTIIKGDETIAQIAMASVLAKAFKDREMLELHALFKEYGWDKNCGYGTKQHIEAIIKL
HHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GATPFHRHSFTLKNRILNPKLLEVEQRLI
CCCCCHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure 
GCVSMTLGIDEAGRGCLAGSLFVAGVACNEKTALEFLKMGLKDSKKLSLKKRFFLEYKI
CCEEEEECCCCCCCCHHHHHHHHEEECCCHHHHHHHHHHCCCCCHHHHHHHHEEEEEEE
KTHGEVGFFVVKKSANEIDSLGLGACLKLAVQEILENGCSLVDEIKIDGNTAFGLNKRYP
ECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHEEECCCEEECCCCCCC
HIQTIIKGDETIAQIAMASVLAKAFKDREMLELHALFKEYGWDKNCGYGTKQHIEAIIKL
HHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GATPFHRHSFTLKNRILNPKLLEVEQRLI
CCCCCHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]