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Identifier: 15645900

GI number: 15645900

Start: 1363131

End: 1363784

Strand: Reverse

Name: Not Available

Synonym: HP1287

Alternate gene names: 15645900

Gene position: 1363784-1363131 (Counterclockwise)

Preceding gene: 15645905

Following gene: 15645898

Centisome position: 81.77

GC content: 38.23

Gene sequence:

>654_bases
ATGCAAGTTTCACAATATCTGTATCAAAATGTGCAATCTATTTGGGGGGATTGTATTTCCCATCCGTTCGTTCAAGGCAT
AGGGCGTGGGACTTTAGAAAGAGATAAATTTCGTTTTTATATCATTCAGGATTATTTGTTCCTTTTAGAATACGCTAAGG
TGTTTGCTTTGGGCGTAGTTAAAGCTTGTGATGAGGCGGTGATGAGGGAGTTTTCTAATGCTATACAAGATATTTTAAAT
AACGAGATGAGTATCCATAACCATTACATTAGAGGACTTCAAATCACTCAAAAAGAATTGCAAAACGCGCGCCCCACTCT
AGCGAATAAATCCTATACAAGCTACATGCTCGCTGAAGGGTTTAAGGGCTCTATCAAAGAAGTTGCGGCGGCTGTTCTAT
CCTGTGGTTGGAGCTATTTAGTGATCGCGCAAAATTTAAGCCAAATCCCCAACGCTTTAGAACATGCCTTTTATGGGCAT
TGGATTAAGGGCTATAGTTCCAAAGAATTTCAAGCGTGCGTAAATTGGAATATTAATTTGCTTGATTCCCTCACTCTCAC
TTCTTCAAAACAAGAAATTGAAAAATTAAAGGACATTTTTATCACTACAAGCGAATACGAATATCTGTTTTGGGATATGG
CGTATCAAAGTTGA

Upstream 100 bases:

>100_bases
GCAGGGCTTACTCATAAATCTTTTAAAAACAAATTTTTATACCCATATTGCGGGCTTTCGTGTTAGAATGCGTGTTGAAT
TGGATTTTAGGAGTGTAAAA

Downstream 100 bases:

>100_bases
ATAAAAGTTTTTGTGCTGTTAGAAAAACGCAAGAGAAGAAGATATCTATCTCTTGCAAAACGATTATTGGGCGTAAGCTT
CTAGTTTGAGCTCAATCTTT

Product: transcriptional regulator (tenA)

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MQVSQYLYQNVQSIWGDCISHPFVQGIGRGTLERDKFRFYIIQDYLFLLEYAKVFALGVVKACDEAVMREFSNAIQDILN
NEMSIHNHYIRGLQITQKELQNARPTLANKSYTSYMLAEGFKGSIKEVAAAVLSCGWSYLVIAQNLSQIPNALEHAFYGH
WIKGYSSKEFQACVNWNINLLDSLTLTSSKQEIEKLKDIFITTSEYEYLFWDMAYQS

Sequences:

>Translated_217_residues
MQVSQYLYQNVQSIWGDCISHPFVQGIGRGTLERDKFRFYIIQDYLFLLEYAKVFALGVVKACDEAVMREFSNAIQDILN
NEMSIHNHYIRGLQITQKELQNARPTLANKSYTSYMLAEGFKGSIKEVAAAVLSCGWSYLVIAQNLSQIPNALEHAFYGH
WIKGYSSKEFQACVNWNINLLDSLTLTSSKQEIEKLKDIFITTSEYEYLFWDMAYQS
>Mature_217_residues
MQVSQYLYQNVQSIWGDCISHPFVQGIGRGTLERDKFRFYIIQDYLFLLEYAKVFALGVVKACDEAVMREFSNAIQDILN
NEMSIHNHYIRGLQITQKELQNARPTLANKSYTSYMLAEGFKGSIKEVAAAVLSCGWSYLVIAQNLSQIPNALEHAFYGH
WIKGYSSKEFQACVNWNINLLDSLTLTSSKQEIEKLKDIFITTSEYEYLFWDMAYQS

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=212, Percent_Identity=26.4150943396226, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325378, Length=213, Percent_Identity=26.2910798122066, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324997, Length=213, Percent_Identity=25.8215962441315, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 25038; Mature: 25038

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSQYLYQNVQSIWGDCISHPFVQGIGRGTLERDKFRFYIIQDYLFLLEYAKVFALGVV
CCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KACDEAVMREFSNAIQDILNNEMSIHNHYIRGLQITQKELQNARPTLANKSYTSYMLAEG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
FKGSIKEVAAAVLSCGWSYLVIAQNLSQIPNALEHAFYGHWIKGYSSKEFQACVNWNINL
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHH
LDSLTLTSSKQEIEKLKDIFITTSEYEYLFWDMAYQS
HHHHHHCCCHHHHHHHHHHHHCCCCCHHHEEEHHHCC
>Mature Secondary Structure
MQVSQYLYQNVQSIWGDCISHPFVQGIGRGTLERDKFRFYIIQDYLFLLEYAKVFALGVV
CCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KACDEAVMREFSNAIQDILNNEMSIHNHYIRGLQITQKELQNARPTLANKSYTSYMLAEG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
FKGSIKEVAAAVLSCGWSYLVIAQNLSQIPNALEHAFYGHWIKGYSSKEFQACVNWNINL
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHH
LDSLTLTSSKQEIEKLKDIFITTSEYEYLFWDMAYQS
HHHHHHCCCHHHHHHHHHHHHCCCCCHHHEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]