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Identifier: 15645859

GI number: 15645859

Start: 1320618

End: 1321157

Strand: Reverse

Name: Not Available

Synonym: HP1245

Alternate gene names: 15645859

Gene position: 1321157-1320618 (Counterclockwise)

Preceding gene: 15645860

Following gene: 15645858

Centisome position: 79.21

GC content: 43.33

Gene sequence:

>540_bases
ATGTTTAATAAAGTGATTATGGTAGGGCGTTTGACCAGGAATGTGGAGTTGAAATATTTGCCTAGCGGTTCGGCTGCGGC
TACAATAGGTTTAGCCACAAGCAGGCGTTTTAAAAAACAAGACGGCACGCTAGGCGAAGAGGTGTGCTTTATAGATGCGC
GTTTGTTTGGGCGAACGGCTGAAATCGCTAACCAGTATTTGAGCAAGGGTTCAAGCGTTTTGATAGAAGGGCGTTTGACT
TATGAGAGTTGGATGGATCAAACGGGCAAAAAAAATTCCCGCCACACTATCACAGCGGACTCGTTGCAATTTATGGATAA
AAAGTCAGACAATCCCCAAGCAAACGCTATGCAAGATAGTATAATGCATGAGAATTCCAACAACGCTTATCCCGCTAATC
ATAACGCTCCCAGCCAAGATCCTTTTAACCAAGCTTATGCGCAAAACGCTTACGCTAAAGAGAATTTACAAGCACAGCCG
TCCAAGTATCAAAACAGCGTGCCTGAAATCAATATTGATGAAGAAGAAATCCCCTTTTAA

Upstream 100 bases:

>100_bases
ATCGCACGCCAAAGAAAAACACGAAAAAACCGAACACACGCATTCTCACCACACAGAGGAAGCAGAAAGCGTAGGATCTC
ATAGCGAATAAGGGCTTATC

Downstream 100 bases:

>100_bases
GGGTTAAAATTAAGGAGACATTATGGAAAGAAAACGCTATTCAAAACGCTATTGCAAATACACTGAAGCTAAAATCAGCT
TTATTGACTATAAAGATTTA

Product: single-stranded DNA-binding protein

Products: NA

Alternate protein names: SSB; Helix-destabilizing protein [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MFNKVIMVGRLTRNVELKYLPSGSAAATIGLATSRRFKKQDGTLGEEVCFIDARLFGRTAEIANQYLSKGSSVLIEGRLT
YESWMDQTGKKNSRHTITADSLQFMDKKSDNPQANAMQDSIMHENSNNAYPANHNAPSQDPFNQAYAQNAYAKENLQAQP
SKYQNSVPEINIDEEEIPF

Sequences:

>Translated_179_residues
MFNKVIMVGRLTRNVELKYLPSGSAAATIGLATSRRFKKQDGTLGEEVCFIDARLFGRTAEIANQYLSKGSSVLIEGRLT
YESWMDQTGKKNSRHTITADSLQFMDKKSDNPQANAMQDSIMHENSNNAYPANHNAPSQDPFNQAYAQNAYAKENLQAQP
SKYQNSVPEINIDEEEIPF
>Mature_179_residues
MFNKVIMVGRLTRNVELKYLPSGSAAATIGLATSRRFKKQDGTLGEEVCFIDARLFGRTAEIANQYLSKGSSVLIEGRLT
YESWMDQTGKKNSRHTITADSLQFMDKKSDNPQANAMQDSIMHENSNNAYPANHNAPSQDPFNQAYAQNAYAKENLQAQP
SKYQNSVPEINIDEEEIPF

Specific function: This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair [H]

COG id: COG0629

COG function: function code L; Single-stranded DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain [H]

Homologues:

Organism=Escherichia coli, GI1790494, Length=90, Percent_Identity=45.5555555555556, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: 1622 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- InterPro:   IPR011344 [H]

Pfam domain/function: PF00436 SSB [H]

EC number: NA

Molecular weight: Translated: 20044; Mature: 20044

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKVIMVGRLTRNVELKYLPSGSAAATIGLATSRRFKKQDGTLGEEVCFIDARLFGRTA
CCCCEEEEEECCCCEEEEECCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEEHHHHHCCHH
EIANQYLSKGSSVLIEGRLTYESWMDQTGKKNSRHTITADSLQFMDKKSDNPQANAMQDS
HHHHHHHHCCCEEEEEEEECHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHH
IMHENSNNAYPANHNAPSQDPFNQAYAQNAYAKENLQAQPSKYQNSVPEINIDEEEIPF
HHHCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCCCCCHHHHCCCCCCCCCHHCCCC
>Mature Secondary Structure
MFNKVIMVGRLTRNVELKYLPSGSAAATIGLATSRRFKKQDGTLGEEVCFIDARLFGRTA
CCCCEEEEEECCCCEEEEECCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEEHHHHHCCHH
EIANQYLSKGSSVLIEGRLTYESWMDQTGKKNSRHTITADSLQFMDKKSDNPQANAMQDS
HHHHHHHHCCCEEEEEEEECHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHH
IMHENSNNAYPANHNAPSQDPFNQAYAQNAYAKENLQAQPSKYQNSVPEINIDEEEIPF
HHHCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCCCCCHHHHCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]