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Identifier: 15645830

GI number: 15645830

Start: 1291604

End: 1293586

Strand: Reverse

Name: Not Available

Synonym: HP1216

Alternate gene names: 15645830

Gene position: 1293586-1291604 (Counterclockwise)

Preceding gene: 15645831

Following gene: 15645829

Centisome position: 77.56

GC content: 37.42

Gene sequence:

>1983_bases
ATGATTTATTGGTTGTATTTGGCGGTCTTTTTTTTGTTGAGCGCATTAGACGCTAAAGAAATCGCTATGCAACGATTTGA
CAAACAAAACCATAAGATTTTTGAAATCCTTGCGGATAAAGTGAGCGCTAAAGACAATGTGATAACCGCATCAGGGAATG
CGATCTTATTGAATTATGATGTGTATATTCTAGCGGACAAGGTGCGTTATGACACTAAAACCAAAGAAGCGTTATTAGAG
GGGAATATCAAGGTTTATAGGGGCGAGGGTTTGCTCGTTAAAACCGATTACGTGAAATTGAGTTTGAATGAAAAATATGA
AATCATTTTCCCCTTTTATGTCCAAGACAGCGTGAGCGGGATTTGGGTGAGCGCGGATATTGCCAGCGGAAAGGATCAAA
AATATAAGGTTAAAAACATGAGCACTTCAGGGTGCAGCATTGATAACCCCATTTGGCATGTCAATGCGACTTCAGGCTCA
TTCAACATGCAAAAATCGCATTTGTCTATGTGGAATCCTAAGATCTATGTCGGTGATATTCCTGTATTGTATTTGCCCTA
TATTTTCATGTCCACGAGCAATAAAAGAACTACTGGGTTTTTATACCCTGAGTTTGGCACTTCCAACTTAGACGGCTTTA
TTTATTTGCAACCCTTTTATTTAGCCCCCAAAAACTCATGGGATATGACCTTTACCCCACAAATCCGCTATAAAAGGGGT
TTTGGCTTGAATTTTGAAGCGCGCTACATTAACTCTAAAAACGACAGGTTTTTATTCAACGCGCGCTATTTTAGGAATTA
CACCCAATATGTCAAACGCTACGATTTGAGGAATCAAAATATCTACGGGTTTGAATTTTTAAGCTCTAGCAGGGACACTT
TACAAAAATACTTCCACCTTAAGTCTAATATTGACAACGGGCATTACATTGACTTTTTATACATGAACGATTTGGACTAT
GTGCGTTTTGAAAAGGTTAATAAGCGTATCACAGACGCCACGCACATGTCTAGGGCGAATTACTATTTGCAAACAGAAAA
CAATTATTACGGCTTGAATATCAAGTATTTTTTAAACCTGAATAAAATCAACAATAACCGCACTTTCCAATCTGTCCCTA
ATTTGCAATACCATAAATATTTAAATTCTTTGTATTTTAGAAATTTGTTGTATTCGGTGGATTATCAGTTTAGAAACACC
GCAAGAGAGATTGGTTATGGCTATGTGCAAAACGCTTTGAATGTGCCGGTGGGCTTGCAATTTTCTTTGTTTAAAAAGTA
TTTGTCTTTAGGGCTTTGGAATGATCTCCAACTATCTAATGTGGCTTTAATGCAATCTAAAAATTCCTTCGTGCCTACGA
TCCCTAATGAATCAAGGGAATTTGGGAATTTTGTGTCTTCAAATTTTTCCATGTATGTCAATACGGATTTGGCTAGAGAA
TACAACAAGCTTTTCCACACGATCCAACTAGAAGCGATTTTCAACATCCCTTATTACACCTTTAAAAACGGCTTATTTTC
TCAAAACATGTATGCTTTAAGCGCGCAAGCCTTAAACAGCTACACTTCGCCTTTATTGAGAGATTATGATTATCAAGGGC
GTTTGTATGACTCGGTGTGGAATCCTAGCAGTATTTTACCTAGCAATGCGAGCAACAAGACGGTGGATTTAACCCTAACG
CAATACCTTTATGGCTTAGGGGGGCAAGAGTTATTGTATTTTAAAATATCGCAACTCATCAATCTTGACGATAAAGTTTC
GCCCTTTAGAATGCCACTAGAGAGCAAGATCGGGTTTTCGCCCTTAACGGGATTGAACATCTTTGGGAATGTCTTTTATT
CGTTTTATCAAAACCGCTTAGAAGAAATCTCTGTGAACGCCAATTACCAACGCAAGTTTTTAAGCTTTAACCTCTCTTAT
TTTTTAAAAAACAATTTTAGCAGTGGGATTAATAGCATTGTAGAAAATCTGCGGATTATTTAA

Upstream 100 bases:

>100_bases
TTTTTCTATGCCCCTTTTTATGGTTTGTTGCGTTTAAAAACCCCTATCATAGGGCGTGGCATGAAGAAAAAAGCAAAAGT
CTTTTGGTATTGTTTTAATC

Downstream 100 bases:

>100_bases
AGGCGGGTTTTAGCAACGACTTTGGCTATTTTTCCATGAGCGCGGATGTGGGTTATGATATTAGAAACAATGTGGTTTTA
AATTGGAATGTGGGGATTTA

Product: hypothetical protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MIYWLYLAVFFLLSALDAKEIAMQRFDKQNHKIFEILADKVSAKDNVITASGNAILLNYDVYILADKVRYDTKTKEALLE
GNIKVYRGEGLLVKTDYVKLSLNEKYEIIFPFYVQDSVSGIWVSADIASGKDQKYKVKNMSTSGCSIDNPIWHVNATSGS
FNMQKSHLSMWNPKIYVGDIPVLYLPYIFMSTSNKRTTGFLYPEFGTSNLDGFIYLQPFYLAPKNSWDMTFTPQIRYKRG
FGLNFEARYINSKNDRFLFNARYFRNYTQYVKRYDLRNQNIYGFEFLSSSRDTLQKYFHLKSNIDNGHYIDFLYMNDLDY
VRFEKVNKRITDATHMSRANYYLQTENNYYGLNIKYFLNLNKINNNRTFQSVPNLQYHKYLNSLYFRNLLYSVDYQFRNT
AREIGYGYVQNALNVPVGLQFSLFKKYLSLGLWNDLQLSNVALMQSKNSFVPTIPNESREFGNFVSSNFSMYVNTDLARE
YNKLFHTIQLEAIFNIPYYTFKNGLFSQNMYALSAQALNSYTSPLLRDYDYQGRLYDSVWNPSSILPSNASNKTVDLTLT
QYLYGLGGQELLYFKISQLINLDDKVSPFRMPLESKIGFSPLTGLNIFGNVFYSFYQNRLEEISVNANYQRKFLSFNLSY
FLKNNFSSGINSIVENLRII

Sequences:

>Translated_660_residues
MIYWLYLAVFFLLSALDAKEIAMQRFDKQNHKIFEILADKVSAKDNVITASGNAILLNYDVYILADKVRYDTKTKEALLE
GNIKVYRGEGLLVKTDYVKLSLNEKYEIIFPFYVQDSVSGIWVSADIASGKDQKYKVKNMSTSGCSIDNPIWHVNATSGS
FNMQKSHLSMWNPKIYVGDIPVLYLPYIFMSTSNKRTTGFLYPEFGTSNLDGFIYLQPFYLAPKNSWDMTFTPQIRYKRG
FGLNFEARYINSKNDRFLFNARYFRNYTQYVKRYDLRNQNIYGFEFLSSSRDTLQKYFHLKSNIDNGHYIDFLYMNDLDY
VRFEKVNKRITDATHMSRANYYLQTENNYYGLNIKYFLNLNKINNNRTFQSVPNLQYHKYLNSLYFRNLLYSVDYQFRNT
AREIGYGYVQNALNVPVGLQFSLFKKYLSLGLWNDLQLSNVALMQSKNSFVPTIPNESREFGNFVSSNFSMYVNTDLARE
YNKLFHTIQLEAIFNIPYYTFKNGLFSQNMYALSAQALNSYTSPLLRDYDYQGRLYDSVWNPSSILPSNASNKTVDLTLT
QYLYGLGGQELLYFKISQLINLDDKVSPFRMPLESKIGFSPLTGLNIFGNVFYSFYQNRLEEISVNANYQRKFLSFNLSY
FLKNNFSSGINSIVENLRII
>Mature_660_residues
MIYWLYLAVFFLLSALDAKEIAMQRFDKQNHKIFEILADKVSAKDNVITASGNAILLNYDVYILADKVRYDTKTKEALLE
GNIKVYRGEGLLVKTDYVKLSLNEKYEIIFPFYVQDSVSGIWVSADIASGKDQKYKVKNMSTSGCSIDNPIWHVNATSGS
FNMQKSHLSMWNPKIYVGDIPVLYLPYIFMSTSNKRTTGFLYPEFGTSNLDGFIYLQPFYLAPKNSWDMTFTPQIRYKRG
FGLNFEARYINSKNDRFLFNARYFRNYTQYVKRYDLRNQNIYGFEFLSSSRDTLQKYFHLKSNIDNGHYIDFLYMNDLDY
VRFEKVNKRITDATHMSRANYYLQTENNYYGLNIKYFLNLNKINNNRTFQSVPNLQYHKYLNSLYFRNLLYSVDYQFRNT
AREIGYGYVQNALNVPVGLQFSLFKKYLSLGLWNDLQLSNVALMQSKNSFVPTIPNESREFGNFVSSNFSMYVNTDLARE
YNKLFHTIQLEAIFNIPYYTFKNGLFSQNMYALSAQALNSYTSPLLRDYDYQGRLYDSVWNPSSILPSNASNKTVDLTLT
QYLYGLGGQELLYFKISQLINLDDKVSPFRMPLESKIGFSPLTGLNIFGNVFYSFYQNRLEEISVNANYQRKFLSFNLSY
FLKNNFSSGINSIVENLRII

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

None

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 77206; Mature: 77206

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYWLYLAVFFLLSALDAKEIAMQRFDKQNHKIFEILADKVSAKDNVITASGNAILLNYD
CEEHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEEE
VYILADKVRYDTKTKEALLEGNIKVYRGEGLLVKTDYVKLSLNEKYEIIFPFYVQDSVSG
EEEEEECCCCCCHHHHHHHCCCEEEEECCEEEEEEEEEEEEECCCEEEEEEEEEECCCCE
IWVSADIASGKDQKYKVKNMSTSGCSIDNPIWHVNATSGSFNMQKSHLSMWNPKIYVGDI
EEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCEEEECCC
PVLYLPYIFMSTSNKRTTGFLYPEFGTSNLDGFIYLQPFYLAPKNSWDMTFTPQIRYKRG
CHHHHCEEEEECCCCCEEEEEEECCCCCCCCEEEEEEEEEECCCCCCCEEECCCEEEECC
FGLNFEARYINSKNDRFLFNARYFRNYTQYVKRYDLRNQNIYGFEFLSSSRDTLQKYFHL
CCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
KSNIDNGHYIDFLYMNDLDYVRFEKVNKRITDATHMSRANYYLQTENNYYGLNIKYFLNL
HCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEEE
NKINNNRTFQSVPNLQYHKYLNSLYFRNLLYSVDYQFRNTAREIGYGYVQNALNVPVGLQ
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHCCCCCCCC
FSLFKKYLSLGLWNDLQLSNVALMQSKNSFVPTIPNESREFGNFVSSNFSMYVNTDLARE
HHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEEECHHHHH
YNKLFHTIQLEAIFNIPYYTFKNGLFSQNMYALSAQALNSYTSPLLRDYDYQGRLYDSVW
HHHHHHEEEEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCEEECCC
NPSSILPSNASNKTVDLTLTQYLYGLGGQELLYFKISQLINLDDKVSPFRMPLESKIGFS
CCCCCCCCCCCCCEEEEEHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCCHHHCCCCC
PLTGLNIFGNVFYSFYQNRLEEISVNANYQRKFLSFNLSYFLKNNFSSGINSIVENLRII
CCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEEEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYWLYLAVFFLLSALDAKEIAMQRFDKQNHKIFEILADKVSAKDNVITASGNAILLNYD
CEEHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEEE
VYILADKVRYDTKTKEALLEGNIKVYRGEGLLVKTDYVKLSLNEKYEIIFPFYVQDSVSG
EEEEEECCCCCCHHHHHHHCCCEEEEECCEEEEEEEEEEEEECCCEEEEEEEEEECCCCE
IWVSADIASGKDQKYKVKNMSTSGCSIDNPIWHVNATSGSFNMQKSHLSMWNPKIYVGDI
EEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCEEEECCC
PVLYLPYIFMSTSNKRTTGFLYPEFGTSNLDGFIYLQPFYLAPKNSWDMTFTPQIRYKRG
CHHHHCEEEEECCCCCEEEEEEECCCCCCCCEEEEEEEEEECCCCCCCEEECCCEEEECC
FGLNFEARYINSKNDRFLFNARYFRNYTQYVKRYDLRNQNIYGFEFLSSSRDTLQKYFHL
CCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
KSNIDNGHYIDFLYMNDLDYVRFEKVNKRITDATHMSRANYYLQTENNYYGLNIKYFLNL
HCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEEE
NKINNNRTFQSVPNLQYHKYLNSLYFRNLLYSVDYQFRNTAREIGYGYVQNALNVPVGLQ
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHCCCCCCCC
FSLFKKYLSLGLWNDLQLSNVALMQSKNSFVPTIPNESREFGNFVSSNFSMYVNTDLARE
HHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEEECHHHHH
YNKLFHTIQLEAIFNIPYYTFKNGLFSQNMYALSAQALNSYTSPLLRDYDYQGRLYDSVW
HHHHHHEEEEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCEEECCC
NPSSILPSNASNKTVDLTLTQYLYGLGGQELLYFKISQLINLDDKVSPFRMPLESKIGFS
CCCCCCCCCCCCCEEEEEHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCCHHHCCCCC
PLTGLNIFGNVFYSFYQNRLEEISVNANYQRKFLSFNLSYFLKNNFSSGINSIVENLRII
CCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEEEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]