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The map label for this gene is tuf

Identifier: 15645819

GI number: 15645819

Start: 1280679

End: 1281878

Strand: Reverse

Name: tuf

Synonym: HP1205

Alternate gene names: 15645819

Gene position: 1281878-1280679 (Counterclockwise)

Preceding gene: 15645821

Following gene: 15645818

Centisome position: 76.86

GC content: 43.83

Gene sequence:

>1200_bases
ATGGCAAAAGAAAAGTTTAACAGAACTAAGCCGCATGTTAATATTGGAACCATTGGGCATGTAGACCATGGTAAAACGAC
TTTGAGTGCAGCGATTTCAGCGGTGCTTTCTTTGAAAGGTCTTGCAGAAATGAAAGACTATGATAATATTGATAACGCCC
CTGAAGAAAAAGAAAGAGGGATCACTATCGCTACTTCTCACATTGAATATGAGACTGAAAACAGACACTATGCGCATGTG
GATTGCCCAGGACACGCTGACTATGTAAAAAACATGATCACCGGTGCGGCGCAAATGGACGGAGCGATTTTGGTTGTTTC
TGCAGCTGATGGCCCTATGCCTCAAACTAGGGAGCATATCTTATTGTCTCGTCAAGTAGGCGTGCCTCACATCGTTGTTT
TCTTAAACAAACAAGACATGGTAGATGACCAAGAATTGTTAGAACTTGTAGAAATGGAAGTGCGCGAATTGTTGAGCGCG
TATGAATTTCCTGGCGATGACACTCCTATCGTAGCGGGTTCAGCTTTAAGAGCTTTAGAAGAAGCAAAGGCTGGTAATGT
GGGTGAATGGGGTGAAAAAGTGCTTAAACTTATGGCTGAAGTGGATGCCTATATCCCTACTCCAGAAAGAGACACTGAAA
AAACTTTCTTGATGCCGGTTGAAGATGTGTTCTCTATTGCGGGTAGAGGGACTGTGGTTACAGGTAGGATTGAAAGAGGC
GTGGTGAAAGTAGGCGATGAAGTGGAAATCGTTGGTATCAGACCTACACAAAAAACGACTGTAACCGGTGTAGAAATGTT
TAGGAAAGAGTTGGAAAAAGGTGAAGCCGGCGATAATGTGGGCGTGCTTTTGAGAGGAACTAAAAAAGAAGAAGTGGAAC
GCGGTATGGTTCTATGCAAACCAGGTTCTATCACTCCGCACAAGAAATTTGAGGGAGAAATTTATGTCCTTTCTAAAGAA
GAAGGCGGGAGACACACTCCATTCTTCACCAATTACCGCCCGCAATTCTATGTGCGCACAACTGATGTGACTGGCTCTAT
CACCCTTCCTGAAGGCGTAGAAATGGTTATGCCTGGCGATAATGTGAAAATCACTGTAGAGTTGATTAGCCCTGTTGCGT
TAGAGTTGGGAACTAAATTTGCGATTCGTGAAGGCGGTAGGACCGTTGGTGCTGGTGTTGTGAGCAATATTATTGAATAA

Upstream 100 bases:

>100_bases
GACAAAAATGTCTTAAATTTTGTGGTAGCATTTAGGAATACTTAGGATTTTGTTTAGTATAATTCTAAAATCCATTTCAA
AAAATTAAGGAGAAATACAA

Downstream 100 bases:

>100_bases
TATTAGCAAAAAGAGAGTTACCATAAAGGGTCATTATGAAAGTTAAAATAGGGTTGAAGTGTTCTGATTGTGAAGATATC
AATTACAGCACAACCAAGAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERGITIATSHIEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKQDMVDDQELLELVEMEVRELLSA
YEFPGDDTPIVAGSALRALEEAKAGNVGEWGEKVLKLMAEVDAYIPTPERDTEKTFLMPVEDVFSIAGRGTVVTGRIERG
VVKVGDEVEIVGIRPTQKTTVTGVEMFRKELEKGEAGDNVGVLLRGTKKEEVERGMVLCKPGSITPHKKFEGEIYVLSKE
EGGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGDNVKITVELISPVALELGTKFAIREGGRTVGAGVVSNIIE

Sequences:

>Translated_399_residues
MAKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERGITIATSHIEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKQDMVDDQELLELVEMEVRELLSA
YEFPGDDTPIVAGSALRALEEAKAGNVGEWGEKVLKLMAEVDAYIPTPERDTEKTFLMPVEDVFSIAGRGTVVTGRIERG
VVKVGDEVEIVGIRPTQKTTVTGVEMFRKELEKGEAGDNVGVLLRGTKKEEVERGMVLCKPGSITPHKKFEGEIYVLSKE
EGGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGDNVKITVELISPVALELGTKFAIREGGRTVGAGVVSNIIE
>Mature_398_residues
AKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERGITIATSHIEYETENRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKQDMVDDQELLELVEMEVRELLSAY
EFPGDDTPIVAGSALRALEEAKAGNVGEWGEKVLKLMAEVDAYIPTPERDTEKTFLMPVEDVFSIAGRGTVVTGRIERGV
VKVGDEVEIVGIRPTQKTTVTGVEMFRKELEKGEAGDNVGVLLRGTKKEEVERGMVLCKPGSITPHKKFEGEIYVLSKEE
GGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGDNVKITVELISPVALELGTKFAIREGGRTVGAGVVSNIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=398, Percent_Identity=57.286432160804, Blast_Score=481, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=438, Percent_Identity=30.365296803653, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI5729864, Length=438, Percent_Identity=30.365296803653, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=29.1479820627803, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=28.2511210762332, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI54607086, Length=321, Percent_Identity=29.2834890965732, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI194018522, Length=335, Percent_Identity=27.7611940298507, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI194018520, Length=335, Percent_Identity=27.7611940298507, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194097354, Length=335, Percent_Identity=27.7611940298507, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI46094014, Length=323, Percent_Identity=28.4829721362229, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4503507, Length=271, Percent_Identity=28.4132841328413, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI82546879, Length=431, Percent_Identity=23.8979118329466, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI157426893, Length=310, Percent_Identity=27.741935483871, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=34.8148148148148, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1790412, Length=398, Percent_Identity=73.6180904522613, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1789737, Length=398, Percent_Identity=73.6180904522613, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI2367247, Length=366, Percent_Identity=31.4207650273224, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI48994988, Length=285, Percent_Identity=30.8771929824561, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789108, Length=266, Percent_Identity=30.4511278195489, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788922, Length=336, Percent_Identity=26.7857142857143, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790835, Length=150, Percent_Identity=30.6666666666667, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=405, Percent_Identity=50.3703703703704, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=44.6153846153846, Blast_Score=340, Evalue=8e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=27.1523178807947, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=27.1523178807947, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=26.7699115044248, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=445, Percent_Identity=27.6404494382022, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=440, Percent_Identity=27.2727272727273, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32566303, Length=444, Percent_Identity=26.8018018018018, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=304, Percent_Identity=27.6315789473684, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=156, Percent_Identity=35.2564102564103, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.0042194092827, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=400, Percent_Identity=64.75, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=446, Percent_Identity=29.5964125560538, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=446, Percent_Identity=29.5964125560538, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322937, Length=328, Percent_Identity=24.6951219512195, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=283, Percent_Identity=28.9752650176678, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=28.9855072463768, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.8148148148148, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=30.0411522633745, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=58.0808080808081, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=58.0808080808081, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=51.025641025641, Blast_Score=413, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24652838, Length=453, Percent_Identity=28.476821192053, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI17137572, Length=453, Percent_Identity=28.476821192053, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI45553807, Length=463, Percent_Identity=27.8617710583153, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45553816, Length=463, Percent_Identity=27.8617710583153, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24651721, Length=463, Percent_Identity=27.8617710583153, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17864154, Length=463, Percent_Identity=27.8617710583153, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=29.8850574712644, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19922690, Length=308, Percent_Identity=32.1428571428571, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45550900, Length=338, Percent_Identity=28.698224852071, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647046, Length=284, Percent_Identity=27.4647887323944, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24647048, Length=284, Percent_Identity=27.4647887323944, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43648; Mature: 43517

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERG
CCCCCCCCCCCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
ITIATSHIEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPHIVVFLNKQDMVDDQELLELVEMEVRELLSAYEFPGDDTPIVAGSALRALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHH
EAKAGNVGEWGEKVLKLMAEVDAYIPTPERDTEKTFLMPVEDVFSIAGRGTVVTGRIERG
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHCCCCEEEEEEECCC
VVKVGDEVEIVGIRPTQKTTVTGVEMFRKELEKGEAGDNVGVLLRGTKKEEVERGMVLCK
EEEECCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEEC
PGSITPHKKFEGEIYVLSKEEGGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGD
CCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCC
NVKITVELISPVALELGTKFAIREGGRTVGAGVVSNIIE
CCEEEEEECCCHHHHHCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERG
CCCCCCCCCCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
ITIATSHIEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPHIVVFLNKQDMVDDQELLELVEMEVRELLSAYEFPGDDTPIVAGSALRALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHH
EAKAGNVGEWGEKVLKLMAEVDAYIPTPERDTEKTFLMPVEDVFSIAGRGTVVTGRIERG
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHCCCCEEEEEEECCC
VVKVGDEVEIVGIRPTQKTTVTGVEMFRKELEKGEAGDNVGVLLRGTKKEEVERGMVLCK
EEEECCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEEC
PGSITPHKKFEGEIYVLSKEEGGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGD
CCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCC
NVKITVELISPVALELGTKFAIREGGRTVGAGVVSNIIE
CCEEEEEECCCHHHHHCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA