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The map label for this gene is trmD

Identifier: 15645762

GI number: 15645762

Start: 1211609

End: 1212298

Strand: Reverse

Name: trmD

Synonym: HP1148

Alternate gene names: 15645762

Gene position: 1212298-1211609 (Counterclockwise)

Preceding gene: 15645763

Following gene: 15645761

Centisome position: 72.69

GC content: 39.57

Gene sequence:

>690_bases
GTGAAATTCAGCGTTTTAACCCTTTTCCCGCAACTCGTATGGCCTTATTTTGAAGATTCTATTTTAAAAAGAGCGTTAGA
AAAAAATCTTTTTGAATTGGAAGTGTTAAACCTTAGGGATTTTAGCGCTAATAAATATCAAAAAGCGGATCACACGCTCA
TTGGTGGGGGTGCAGGGCAGATTTTAGACCCTGAGATGGTAGAAAACGCGCTCCACTCTGTTAAAAACCCTAAACACACG
ATTTTTTTAAGCGCGGTGGGCAAGCCTTTCAAGCAAACAGATGCGATGCGTTTGGCTCAAAAAAAGCATGTCGTTTTGGT
GTGCGGGCGTTATGAGGGCTTTGATGAACGCTCTATTGAACTTGGTGCTGATGAGGTTTTTTGTATAGGCGATTTTATTT
TAACAGGGGGCGAGCTTGGGGCGTTGTGCTTGATAGATAGTATCGCTCGCCACATTCAAGGGGTTTTGGGTAACGCTCAA
TCTTTAGAAAATGAGAGCTTTGAAAACCATTATTTGGAAGCCCCTAATTTCGCTAACGCTGTTTTTAAATCCAAAGAAAT
CAATAAAATCCCTGCACCTTTAGAATATTCTAAAGGAAATCATGCTAAAATCAAGCAACTGAAACTTGATTTGTCAAAAT
TAAGGACGAAATTTTACCGCCTTGATTTATTCAAACAGCACAAATCATGA

Upstream 100 bases:

>100_bases
TTTTTTAATCCCTTATAGGGATTTTTATATCCAAGAAATCCTTTTGCAAGACAAAAAAATAACCACCCATAACGCTAAAA
CGCTTTTAGAGAATAGTTGA

Downstream 100 bases:

>100_bases
AATTGACATTAAGGAAAACACATGAAAAACCGCTACATTCAGCAGTTTGAAGACGCTCAATTAAAAGACAAGACCATGCC
AGCATTTAAAGCCGGCGATA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKFSVLTLFPQLVWPYFEDSILKRALEKNLFELEVLNLRDFSANKYQKADHTLIGGGAGQILDPEMVENALHSVKNPKHT
IFLSAVGKPFKQTDAMRLAQKKHVVLVCGRYEGFDERSIELGADEVFCIGDFILTGGELGALCLIDSIARHIQGVLGNAQ
SLENESFENHYLEAPNFANAVFKSKEINKIPAPLEYSKGNHAKIKQLKLDLSKLRTKFYRLDLFKQHKS

Sequences:

>Translated_229_residues
MKFSVLTLFPQLVWPYFEDSILKRALEKNLFELEVLNLRDFSANKYQKADHTLIGGGAGQILDPEMVENALHSVKNPKHT
IFLSAVGKPFKQTDAMRLAQKKHVVLVCGRYEGFDERSIELGADEVFCIGDFILTGGELGALCLIDSIARHIQGVLGNAQ
SLENESFENHYLEAPNFANAVFKSKEINKIPAPLEYSKGNHAKIKQLKLDLSKLRTKFYRLDLFKQHKS
>Mature_229_residues
MKFSVLTLFPQLVWPYFEDSILKRALEKNLFELEVLNLRDFSANKYQKADHTLIGGGAGQILDPEMVENALHSVKNPKHT
IFLSAVGKPFKQTDAMRLAQKKHVVLVCGRYEGFDERSIELGADEVFCIGDFILTGGELGALCLIDSIARHIQGVLGNAQ
SLENESFENHYLEAPNFANAVFKSKEINKIPAPLEYSKGNHAKIKQLKLDLSKLRTKFYRLDLFKQHKS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=229, Percent_Identity=32.7510917030568, Blast_Score=133, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 25879; Mature: 25879

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSVLTLFPQLVWPYFEDSILKRALEKNLFELEVLNLRDFSANKYQKADHTLIGGGAGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCC
ILDPEMVENALHSVKNPKHTIFLSAVGKPFKQTDAMRLAQKKHVVLVCGRYEGFDERSIE
CCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCEE
LGADEVFCIGDFILTGGELGALCLIDSIARHIQGVLGNAQSLENESFENHYLEAPNFANA
CCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCCCHHHH
VFKSKEINKIPAPLEYSKGNHAKIKQLKLDLSKLRTKFYRLDLFKQHKS
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSVLTLFPQLVWPYFEDSILKRALEKNLFELEVLNLRDFSANKYQKADHTLIGGGAGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCC
ILDPEMVENALHSVKNPKHTIFLSAVGKPFKQTDAMRLAQKKHVVLVCGRYEGFDERSIE
CCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCEE
LGADEVFCIGDFILTGGELGALCLIDSIARHIQGVLGNAQSLENESFENHYLEAPNFANA
CCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCCCHHHH
VFKSKEINKIPAPLEYSKGNHAKIKQLKLDLSKLRTKFYRLDLFKQHKS
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]