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Identifier: 15645573

GI number: 15645573

Start: 1015786

End: 1016967

Strand: Reverse

Name: Not Available

Synonym: HP0957

Alternate gene names: 15645573

Gene position: 1016967-1015786 (Counterclockwise)

Preceding gene: 15645574

Following gene: 15645572

Centisome position: 60.97

GC content: 38.41

Gene sequence:

>1182_bases
TTGTTTAAGTTTTTCTACCTTTTATTTTTGACTTTGGGGCATCTTCTTGGTGCACCTTTCATCTTCTTTTGGAGTTTTAA
GGAAAAATACCGCCATTCTTTGAAAGCCCGTTTTTTTCTCAAAGACAATTTTTTAAAAAGCGAGCCGGTTTTTTGGTTCC
ATGCATGCTCTTATGGGGAGGTCAAATCCTTAGAGCCAATCATTCACGCTTTAAAAGAGCCGATTTTAATCAGCGTTACC
ACTAATACCGGCTTTGAATTAGCCGCTCAAACTTATCAGCATTCTAAGCATATAGAAGTGCGTTACCTGCCTTTTGAAAC
CCTATTATTTGCATGGAAAAAAAACTTAAAACGCTTAAAAACTTTGGTGGTTACAGAAGCGGAATTGTGGTTTAATGTGT
TTGATACGGCTCAAAAATTAGGGGCAAAAACCATGCTCATTAACGCTCGAATCAGCGTTCGTTCTTACCCCAAGTATCAG
CGTTTTTCTTTCTTTTATGCGCTTTTATTCAAACGCATTGATTTGATTTTAGCGCAAAGCAAGGAGGATAAAAAGCGTTT
GTTGAATCTAGGGGCAAAAAAAGTGGTGGATTTTTTGAATATCAAGCGTTTTTCAAAGCCTGTGATCACTTCGTTTTACC
CTAAAAACCCAAGCGCTTTAAACATTGTTTTAGCCAGCACGCATGAGGGCGAAGAGGAATTAGGGTTAAAAGCGTTTTTG
GAGCTAAAAAAGACTTTTAAAAACGCAAGACTGATTGTTGTGCCGCGCCACCCTGAGCGTTTTAAAAGCGTGCAAGATTT
ATTGCAAAATACCTTAAAAACGACGCCTTTTAGTTGGGAATGTTTTTCTTCAAAGGGTTTTGTGGAATGCGATATTTTGT
TAGTGGATAGCTTGGGGGAATTGAATAATTTCTACCAAATCGCAGACATTGTCATTTTAGGGGGTTCGTTTGTCAAAATG
GGGGGGCATAACCCTTTAGAGCCGGCGTTTTTTAATGCGCGCTTGATCACAGGGGAGTATATTTTCAACCAGGTAGCGTT
GTTTGAATTAGTCAAGCCTTATAAAATCGTTCCAAAAGAGGATCTGTTAGACGCTCTTTTAGATTACAAAAATTTAGGCA
TGGCGCGTTTTTTAGAAAACGGGCATGATTTAAACGAATTGCTCGCTTTCATTAAACATTAA

Upstream 100 bases:

>100_bases
TTGCCCGTATTGCGGGCGTATTTTATACGCTGAGGGCGCGTATGAAAACAGCGCTCAACCTCCAAAAGAAAGCCAAGAAG
AAAGCCAAGAATTAGTTTGA

Downstream 100 bases:

>100_bases
AAAGGATAAACATGGAAAAAGCTTATAAAATATTGAGCGTTCAAGAAAACATTTCGCATAAAAAAGCCAAAGCTTTGATT
GACTCTGGGTTAGTGAGTAT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KdtA protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MFKFFYLLFLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGEVKSLEPIIHALKEPILISVT
TNTGFELAAQTYQHSKHIEVRYLPFETLLFAWKKNLKRLKTLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQ
RFSFFYALLFKRIDLILAQSKEDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSALNIVLASTHEGEEELGLKAFL
ELKKTFKNARLIVVPRHPERFKSVQDLLQNTLKTTPFSWECFSSKGFVECDILLVDSLGELNNFYQIADIVILGGSFVKM
GGHNPLEPAFFNARLITGEYIFNQVALFELVKPYKIVPKEDLLDALLDYKNLGMARFLENGHDLNELLAFIKH

Sequences:

>Translated_393_residues
MFKFFYLLFLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGEVKSLEPIIHALKEPILISVT
TNTGFELAAQTYQHSKHIEVRYLPFETLLFAWKKNLKRLKTLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQ
RFSFFYALLFKRIDLILAQSKEDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSALNIVLASTHEGEEELGLKAFL
ELKKTFKNARLIVVPRHPERFKSVQDLLQNTLKTTPFSWECFSSKGFVECDILLVDSLGELNNFYQIADIVILGGSFVKM
GGHNPLEPAFFNARLITGEYIFNQVALFELVKPYKIVPKEDLLDALLDYKNLGMARFLENGHDLNELLAFIKH
>Mature_393_residues
MFKFFYLLFLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGEVKSLEPIIHALKEPILISVT
TNTGFELAAQTYQHSKHIEVRYLPFETLLFAWKKNLKRLKTLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQ
RFSFFYALLFKRIDLILAQSKEDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSALNIVLASTHEGEEELGLKAFL
ELKKTFKNARLIVVPRHPERFKSVQDLLQNTLKTTPFSWECFSSKGFVECDILLVDSLGELNNFYQIADIVILGGSFVKM
GGHNPLEPAFFNARLITGEYIFNQVALFELVKPYKIVPKEDLLDALLDYKNLGMARFLENGHDLNELLAFIKH

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Inner Membrane-Anchored [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=345, Percent_Identity=32.1739130434783, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007507
- InterPro:   IPR003358 [H]

Pfam domain/function: PF04413 Glycos_transf_N; PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 45623; Mature: 45623

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKFFYLLFLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGE
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCC
VKSLEPIIHALKEPILISVTTNTGFELAAQTYQHSKHIEVRYLPFETLLFAWKKNLKRLK
CHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
TLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQRFSFFYALLFKRIDLILAQS
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
KEDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSALNIVLASTHEGEEELGLKAFL
CHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHH
ELKKTFKNARLIVVPRHPERFKSVQDLLQNTLKTTPFSWECFSSKGFVECDILLVDSLGE
HHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCHHH
LNNFYQIADIVILGGSFVKMGGHNPLEPAFFNARLITGEYIFNQVALFELVKPYKIVPKE
HHHHHHHHHHEEECCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHH
DLLDALLDYKNLGMARFLENGHDLNELLAFIKH
HHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MFKFFYLLFLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGE
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCC
VKSLEPIIHALKEPILISVTTNTGFELAAQTYQHSKHIEVRYLPFETLLFAWKKNLKRLK
CHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
TLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQRFSFFYALLFKRIDLILAQS
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
KEDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSALNIVLASTHEGEEELGLKAFL
CHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHH
ELKKTFKNARLIVVPRHPERFKSVQDLLQNTLKTTPFSWECFSSKGFVECDILLVDSLGE
HHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCHHH
LNNFYQIADIVILGGSFVKMGGHNPLEPAFFNARLITGEYIFNQVALFELVKPYKIVPKE
HHHHHHHHHHEEECCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHH
DLLDALLDYKNLGMARFLENGHDLNELLAFIKH
HHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11889109 [H]