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Identifier: 15645552

GI number: 15645552

Start: 996532

End: 997701

Strand: Reverse

Name: Not Available

Synonym: HP0936

Alternate gene names: 15645552

Gene position: 997701-996532 (Counterclockwise)

Preceding gene: 15645553

Following gene: 15645540

Centisome position: 59.82

GC content: 38.38

Gene sequence:

>1170_bases
ATGGCCCACTTTGGGGATAGATTCGGCCGTAAAAACATGTTCATGCTCTCTATTTTATTGATGGTGATCCCAACCTTTGC
GTTAGCTTTGATGCCAACTTTTAATCATTTGGTGGGTTTTGGCGTTGATAACATGGGGCTTAGCCTAAAAAACGCTCATT
ATCTTGGTTATATAGCTCCTGTTTTTTTGGTGTTTGTTAGGATCTGTCAAGGCGTTGCTGTGGGTGGTGAATTGCCTGGA
GCTTGGGTTTTTGTCCATGAACATGCCCCGCAAGGACAAAAAAACACTTATATTGGTTTTTTAACCGCTTCTGTAGTTTC
TGGGATTTTGCTTGGGAGTTTGGTTTATATAGGGATTTACATGGTTTTTGACAAGCCTGTTGTTGAAGATTGGGCTTGGA
GGGTTGCCTTTGGGCTTGGAGGGATTTTTGGTATCATTTCTGTGTATTTGAGGCGCTTTTTAGAAGAAACTCCTGTTTTT
CAGCAAATGAAGCAAGACAATGCCTTAGTCAAATTCCCGCTTAAAGAGGTGTTTAAAAACTCTCTCTTTGGTATATCAAT
CTCCATGCTTATCACTTGGGTTTTAACCGCTTGTATTTTGATTTTTATCCTTTTTGTTCCCAATTTTACCCTTACGCATC
CTAATTTTAATTTCACTCCGTTTGAAAAAACCTATTTTCAAATTTTAGGACTTGTTGGTATTGTAAGTTCTATTATTTTA
ACCGGGTTTTTAGCCGATAAAATCAAACCGCACAAAGTTTGTATGGCTTTTAGCGTGGCCTTTGCCTTTTTTGGCTTTTT
ATTCTTTAAAGAATTTTATTCTAACGCGCCAAGTTTAGTCAATACCATAGTTTTATACTTTTTAGCTTGCTTTTGCGCTG
GTATTATGAATTTTTGCCCCATTTTTATGAGCGATGTGTTTAGCGCCAAAATCCGTTTTAGCGGGATTTCTTTCGCTTAT
AACATAGCCTATGCTATAACCGCTGGCTTTACCCCTCAACTTTCAAGCTGGTTGAATGCAAAAGCTATAGCAGCGCCTGA
AAGCTTGCAAAGTTATGGTTTAAGCTTTTATATTTTTGTGGTTGCTTTAATCGCTTTTATCGTATCGCTTTTAATGGCGC
CAATTTACAACAAATCTAATACCCAACACGAAGTGTCGCCCACAGCATGA

Upstream 100 bases:

>100_bases
ACACTTTTTCCCAAACACGCTTAGCCCCATTTGGTCTGAAATCAACACTTATGGTATCTTTGCTGCAGGTTATCTGGCGC
GCCCGCTTGGCGGCATAGTG

Downstream 100 bases:

>100_bases
AGGATGCGCAAACCTTTGAGTGATTCAATCAAAACTTAGCCGATAGCGTTGGTGGGTTTTGGTGATGATAAAATTTTTTC
GTTTTGAATCTCATTTTGTT

Product: proline/betaine transporter (proP)

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MAHFGDRFGRKNMFMLSILLMVIPTFALALMPTFNHLVGFGVDNMGLSLKNAHYLGYIAPVFLVFVRICQGVAVGGELPG
AWVFVHEHAPQGQKNTYIGFLTASVVSGILLGSLVYIGIYMVFDKPVVEDWAWRVAFGLGGIFGIISVYLRRFLEETPVF
QQMKQDNALVKFPLKEVFKNSLFGISISMLITWVLTACILIFILFVPNFTLTHPNFNFTPFEKTYFQILGLVGIVSSIIL
TGFLADKIKPHKVCMAFSVAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCPIFMSDVFSAKIRFSGISFAY
NIAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFVVALIAFIVSLLMAPIYNKSNTQHEVSPTA

Sequences:

>Translated_389_residues
MAHFGDRFGRKNMFMLSILLMVIPTFALALMPTFNHLVGFGVDNMGLSLKNAHYLGYIAPVFLVFVRICQGVAVGGELPG
AWVFVHEHAPQGQKNTYIGFLTASVVSGILLGSLVYIGIYMVFDKPVVEDWAWRVAFGLGGIFGIISVYLRRFLEETPVF
QQMKQDNALVKFPLKEVFKNSLFGISISMLITWVLTACILIFILFVPNFTLTHPNFNFTPFEKTYFQILGLVGIVSSIIL
TGFLADKIKPHKVCMAFSVAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCPIFMSDVFSAKIRFSGISFAY
NIAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFVVALIAFIVSLLMAPIYNKSNTQHEVSPTA
>Mature_388_residues
AHFGDRFGRKNMFMLSILLMVIPTFALALMPTFNHLVGFGVDNMGLSLKNAHYLGYIAPVFLVFVRICQGVAVGGELPGA
WVFVHEHAPQGQKNTYIGFLTASVVSGILLGSLVYIGIYMVFDKPVVEDWAWRVAFGLGGIFGIISVYLRRFLEETPVFQ
QMKQDNALVKFPLKEVFKNSLFGISISMLITWVLTACILIFILFVPNFTLTHPNFNFTPFEKTYFQILGLVGIVSSIILT
GFLADKIKPHKVCMAFSVAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCPIFMSDVFSAKIRFSGISFAYN
IAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFVVALIAFIVSLLMAPIYNKSNTQHEVSPTA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=388, Percent_Identity=27.0618556701031, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1788292, Length=378, Percent_Identity=26.984126984127, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1789941, Length=347, Percent_Identity=24.7838616714697, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788942, Length=394, Percent_Identity=24.8730964467005, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 43272; Mature: 43141

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AHFGDRFGRKNMFMLSILLMVIPTFALALMPTFNHLVGFGVDNMGLSLKNAHYLGYIAP
CCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
VFLVFVRICQGVAVGGELPGAWVFVHEHAPQGQKNTYIGFLTASVVSGILLGSLVYIGIY
HHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MVFDKPVVEDWAWRVAFGLGGIFGIISVYLRRFLEETPVFQQMKQDNALVKFPLKEVFKN
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCEEECCHHHHHHH
SLFGISISMLITWVLTACILIFILFVPNFTLTHPNFNFTPFEKTYFQILGLVGIVSSIIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
TGFLADKIKPHKVCMAFSVAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCP
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IFMSDVFSAKIRFSGISFAYNIAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFV
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHCCHHHHHHHCHHHHHHH
VALIAFIVSLLMAPIYNKSNTQHEVSPTA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]