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Identifier: 15645429

GI number: 15645429

Start: 864477

End: 865079

Strand: Reverse

Name: Not Available

Synonym: HP0810

Alternate gene names: 15645429

Gene position: 865079-864477 (Counterclockwise)

Preceding gene: 15645430

Following gene: 15645428

Centisome position: 51.87

GC content: 34.99

Gene sequence:

>603_bases
ATGCCAAATCATCAGCCAGTAAAAAAATTTAAGATTATTGGGGGGGCTTGTAAGGGATTAGGCTTGAATTTGCCTAACAT
TTCTAGCACGCGCCCCACCAAAGCGATCGTAAGAGAGTCGTTTTTTAACACCTTGCAAGCAGAAATTAATGGAGCGCATT
TTATAGAAGTGTTTTCAGGCAGCGCTTCTATGGGTTTAGAGGCTTTGAGTAGGGGGGCTAAAAGTGCGGTGTTTTTTGAA
CAAAACAAAAGCGCTTATAAGACGCTTTTAGAAAATATTTCCCTTTTTAAAAACCGCTTGAAAAAAGAAATGGAAATTCA
AACCTTTTTAGATGACGCTTTCAAGCTTTTGCCCACGCTGTGTTTAAAAAATGGCGTTTTGAATATTATTTATTTGGATC
CTCCTTTTGAAACAAGTGGGTTTTTAGGGATTTATGAAAAGTGTTTTCAAGCTTTAGAAAGGTTATTGAAACGCTTTAAT
CCAAAAAATCTTTTAGTGGTTTTTGAGCATGAAAGCATGCATGAAATGCCTAAAAGTCTTGTAACTTTAGCTATAATCAA
ACAAAAAAAATTTGGAAAAACCACTTTAACTTATTTTCAATAG

Upstream 100 bases:

>100_bases
TTTATCGCGCTTTAAAGACGAGCGAAAAACCTTGCGAAGAAATCCAATTTTCTAAAGAGCAAAAGATTAAGCAATTACTA
GAAGAATACACCCACAAATT

Downstream 100 bases:

>100_bases
GAATAGGCATGGCAGAAGAACAAGAAAATACCGCGCAACAACCCCCCAAAAAAAGCAAAGCCCTTTTATTTGTCATTATT
GGAAGCGTGTTAGTGATGCT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MPNHQPVKKFKIIGGACKGLGLNLPNISSTRPTKAIVRESFFNTLQAEINGAHFIEVFSGSASMGLEALSRGAKSAVFFE
QNKSAYKTLLENISLFKNRLKKEMEIQTFLDDAFKLLPTLCLKNGVLNIIYLDPPFETSGFLGIYEKCFQALERLLKRFN
PKNLLVVFEHESMHEMPKSLVTLAIIKQKKFGKTTLTYFQ

Sequences:

>Translated_200_residues
MPNHQPVKKFKIIGGACKGLGLNLPNISSTRPTKAIVRESFFNTLQAEINGAHFIEVFSGSASMGLEALSRGAKSAVFFE
QNKSAYKTLLENISLFKNRLKKEMEIQTFLDDAFKLLPTLCLKNGVLNIIYLDPPFETSGFLGIYEKCFQALERLLKRFN
PKNLLVVFEHESMHEMPKSLVTLAIIKQKKFGKTTLTYFQ
>Mature_199_residues
PNHQPVKKFKIIGGACKGLGLNLPNISSTRPTKAIVRESFFNTLQAEINGAHFIEVFSGSASMGLEALSRGAKSAVFFEQ
NKSAYKTLLENISLFKNRLKKEMEIQTFLDDAFKLLPTLCLKNGVLNIIYLDPPFETSGFLGIYEKCFQALERLLKRFNP
KNLLVVFEHESMHEMPKSLVTLAIIKQKKFGKTTLTYFQ

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=201, Percent_Identity=29.8507462686567, Blast_Score=87, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22597; Mature: 22466

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNHQPVKKFKIIGGACKGLGLNLPNISSTRPTKAIVRESFFNTLQAEINGAHFIEVFSG
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECC
SASMGLEALSRGAKSAVFFEQNKSAYKTLLENISLFKNRLKKEMEIQTFLDDAFKLLPTL
CHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLKNGVLNIIYLDPPFETSGFLGIYEKCFQALERLLKRFNPKNLLVVFEHESMHEMPKSL
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHH
VTLAIIKQKKFGKTTLTYFQ
HHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
PNHQPVKKFKIIGGACKGLGLNLPNISSTRPTKAIVRESFFNTLQAEINGAHFIEVFSG
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECC
SASMGLEALSRGAKSAVFFEQNKSAYKTLLENISLFKNRLKKEMEIQTFLDDAFKLLPTL
CHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLKNGVLNIIYLDPPFETSGFLGIYEKCFQALERLLKRFNPKNLLVVFEHESMHEMPKSL
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHH
VTLAIIKQKKFGKTTLTYFQ
HHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]