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Identifier: 15645344

GI number: 15645344

Start: 776481

End: 777473

Strand: Reverse

Name: Not Available

Synonym: HP0723

Alternate gene names: 15645344

Gene position: 777473-776481 (Counterclockwise)

Preceding gene: 15646201

Following gene: 15645340

Centisome position: 46.61

GC content: 45.72

Gene sequence:

>993_bases
ATGGCTCAAAATTTACCCACCATTGCTTTACTGGCGACAGGGGGGACGATTGCAGGGAGTGGTGCGAGCGCGAGTTTGGG
TAGTTATAAGAGTGGTGAGTTGGGCATCAAAGAGCTTTTGAAGGCTATCCCTAGTCTTAACAGACTCGCTCGCATTCAAG
GGGAGCAGATTTCTAACATCGGCTCACAAGACATGAATGAAGAGGTATGGTTCAAGCTCGCCAAACGTGCCCAAGAATTG
CTAGATGATAGCCGTATTCAAGGCGTGGTCATCACGCATGGCACGGACACTTTAGAAGAGAGCGCGTATTTTTTAAACTT
AGTTTTACGCTCCACAAAACCGGTCGTGCTGGTGGGAGCGATGCGTAATGCTGCTTCTTTGAGCGCGGATGGGGCTTTGA
ATTTATATAATGCTGTGAGCGTAGCGCTCAATGAAAAAAGTGCGAATAAAGGCGTGTTAGTGGTGATGGACGATAATATT
TTTAGCGCTAGAGAAGTGATTAAAACGCACACCACCCACACTTCCACCTTTAAAGCCTTAAATAGCGGCGCGATAGGGAG
CGTGTATTATGGCAAAACGCGCTATTACATGCAGCCTTTGAGAAAACACACCACAGAGAGCGAATTTTCCCTTTCACAAC
TCAAAACCCCCCTGCCTAAAGTGGATATTATTTACACGCATGCTGGCATGACCCCTGATTTATTCCAAGCGAGCCTAAAC
TCGCATGCAAAAGGCGTTGTGATAGCCGGGGTGGGTAATGGGAATGTGAGCGCTGGGTTTTTAAAAGCGATGCAAGAAGC
GAGCCAAATGGGGGTGGTTATTGTTCGTTCTAGCAGGGTAAATAGCGGTGAGATTACTTCAGGCGAGATTGATGACAAGG
CCTTCATCACAAGCGACAATTTAAACCCCCAAAAAGCTAGGGTGCTTTTACAACTCGCTTTAACTAAAACAAATAATAAA
GAAAAAATCCAAGAAATGTTTGAAGAGTATTGA

Upstream 100 bases:

>100_bases
GTTTTACTATACTATAAAATCTTTTAATTTCAAAAGGTGGTCCATAATGAGAATATTTTTGAAATTGTTGATTCTTTTAT
TTTGTTTGAAGGGGCAGGTT

Downstream 100 bases:

>100_bases
AAGATTCTCTTAAATCACCCAATTATCAAAGATAATTGGGTGATTTGGTTTATTTTGTTTTTGACTTTATTTTCATCAAA
TATTGATAACTATCAAAATA

Product: L-asparaginase II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAQNLPTIALLATGGTIAGSGASASLGSYKSGELGIKELLKAIPSLNRLARIQGEQISNIGSQDMNEEVWFKLAKRAQEL
LDDSRIQGVVITHGTDTLEESAYFLNLVLRSTKPVVLVGAMRNAASLSADGALNLYNAVSVALNEKSANKGVLVVMDDNI
FSAREVIKTHTTHTSTFKALNSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDLFQASLN
SHAKGVVIAGVGNGNVSAGFLKAMQEASQMGVVIVRSSRVNSGEITSGEIDDKAFITSDNLNPQKARVLLQLALTKTNNK
EKIQEMFEEY

Sequences:

>Translated_330_residues
MAQNLPTIALLATGGTIAGSGASASLGSYKSGELGIKELLKAIPSLNRLARIQGEQISNIGSQDMNEEVWFKLAKRAQEL
LDDSRIQGVVITHGTDTLEESAYFLNLVLRSTKPVVLVGAMRNAASLSADGALNLYNAVSVALNEKSANKGVLVVMDDNI
FSAREVIKTHTTHTSTFKALNSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDLFQASLN
SHAKGVVIAGVGNGNVSAGFLKAMQEASQMGVVIVRSSRVNSGEITSGEIDDKAFITSDNLNPQKARVLLQLALTKTNNK
EKIQEMFEEY
>Mature_329_residues
AQNLPTIALLATGGTIAGSGASASLGSYKSGELGIKELLKAIPSLNRLARIQGEQISNIGSQDMNEEVWFKLAKRAQELL
DDSRIQGVVITHGTDTLEESAYFLNLVLRSTKPVVLVGAMRNAASLSADGALNLYNAVSVALNEKSANKGVLVVMDDNIF
SAREVIKTHTTHTSTFKALNSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDLFQASLNS
HAKGVVIAGVGNGNVSAGFLKAMQEASQMGVVIVRSSRVNSGEITSGEIDDKAFITSDNLNPQKARVLLQLALTKTNNKE
KIQEMFEEY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=329, Percent_Identity=49.2401215805471, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1788065, Length=288, Percent_Identity=28.125, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=329, Percent_Identity=37.6899696048632, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6323187, Length=329, Percent_Identity=37.6899696048632, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6323186, Length=329, Percent_Identity=37.6899696048632, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6323184, Length=329, Percent_Identity=37.6899696048632, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6320527, Length=332, Percent_Identity=32.8313253012048, Blast_Score=183, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35539; Mature: 35408

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQNLPTIALLATGGTIAGSGASASLGSYKSGELGIKELLKAIPSLNRLARIQGEQISNI
CCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHCC
GSQDMNEEVWFKLAKRAQELLDDSRIQGVVITHGTDTLEESAYFLNLVLRSTKPVVLVGA
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEE
MRNAASLSADGALNLYNAVSVALNEKSANKGVLVVMDDNIFSAREVIKTHTTHTSTFKAL
CCCCCCCCCCCCEEHHHHEEEEEECCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHH
NSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDLFQASLN
CCCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHC
SHAKGVVIAGVGNGNVSAGFLKAMQEASQMGVVIVRSSRVNSGEITSGEIDDKAFITSDN
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCC
LNPQKARVLLQLALTKTNNKEKIQEMFEEY
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AQNLPTIALLATGGTIAGSGASASLGSYKSGELGIKELLKAIPSLNRLARIQGEQISNI
CCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHCC
GSQDMNEEVWFKLAKRAQELLDDSRIQGVVITHGTDTLEESAYFLNLVLRSTKPVVLVGA
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEE
MRNAASLSADGALNLYNAVSVALNEKSANKGVLVVMDDNIFSAREVIKTHTTHTSTFKAL
CCCCCCCCCCCCEEHHHHEEEEEECCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHH
NSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDLFQASLN
CCCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHC
SHAKGVVIAGVGNGNVSAGFLKAMQEASQMGVVIVRSSRVNSGEITSGEIDDKAFITSDN
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCC
LNPQKARVLLQLALTKTNNKEKIQEMFEEY
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]