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Identifier: 15644699

GI number: 15644699

Start: 73446

End: 74210

Strand: Reverse

Name: Not Available

Synonym: HP0069

Alternate gene names: 15644699

Gene position: 74210-73446 (Counterclockwise)

Preceding gene: 15644700

Following gene: 15644698

Centisome position: 4.45

GC content: 40.92

Gene sequence:

>765_bases
ATGGATAAAGGAAAAAGCGTGAAAAGCACTGAAAAAAGCGTGGGTATGCCCCCAAAAACTCCAAAGACAGACAACAACGC
TCATGTGGATAACGAATTTCTGATTCTGCAAGTCAATGATGCGGTGTTCCCTATTGGATCTTACACGCATTCTTTTGGGC
TAGAAACTTACATCCAGCAAAAAAAGGTTACCAATAAAGAAAGCGCTTTAGAATATTTAAAAGCCAATCTCTCTAGCCAG
TTCCTTTACACGGAAATGCTGAGCTTGAAATTAACCTATGAAAGCGCCCTCCAACAAGATTTAAAAAAGATCTTAGGGGT
TGAAGAAGTCATTATGCTATCCACAAGCCCCATGGAATTACGATTAGCCAATCAAAAGCTGGGCAATCGTTTCATTAAAA
CCTTACAAGCCATGAACGAATTAGACATGGGCGAATTTTTTAACGCTTACGCTCAAAAAACCAAAGATCCCACCCATGCC
ACTAGCTATGGCGTTTTTGCGGCAAGTTTGGGGATTGAATTGAAAAAGGCTTTAAGGCATTATCTTTATGCGCAAACTTC
TAACATGGTGATCAACTGCGTTAAAAGCGTCCCTTTATCTCAAAACGACGGGCAAAAAATCTTATTGAGCTTGCAAAGCC
CTTTTAACCAGCTCATAGAAAAAACCCTAGAACTAGACGAAAGCCACCTGTGCACGGCAAGCGTTCAAAACGACATTAAG
GCGATGCAGCATGAGAGTTTATACTCGCGCCTTTATATGTCTTGA

Upstream 100 bases:

>100_bases
TTAACCGTGAGCATGCCCCATAGTGAGCCTAATTTTAAGGTCTCACTAGCGAGCGATTTTAAAGTGGTCGTAAAATAGAA
AAGAAATAGAAAAATAACAA

Downstream 100 bases:

>100_bases
ATTTTAATCTCAAATTGAAAGGAATTTTATGGTAAAAATTGGAGTTTGTGGTCCTGTAGGAAGCGGTAAAACCGCCTTGA
TTGAAGCTTTAACGCGCCAC

Product: urease accessory protein UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MDKGKSVKSTEKSVGMPPKTPKTDNNAHVDNEFLILQVNDAVFPIGSYTHSFGLETYIQQKKVTNKESALEYLKANLSSQ
FLYTEMLSLKLTYESALQQDLKKILGVEEVIMLSTSPMELRLANQKLGNRFIKTLQAMNELDMGEFFNAYAQKTKDPTHA
TSYGVFAASLGIELKKALRHYLYAQTSNMVINCVKSVPLSQNDGQKILLSLQSPFNQLIEKTLELDESHLCTASVQNDIK
AMQHESLYSRLYMS

Sequences:

>Translated_254_residues
MDKGKSVKSTEKSVGMPPKTPKTDNNAHVDNEFLILQVNDAVFPIGSYTHSFGLETYIQQKKVTNKESALEYLKANLSSQ
FLYTEMLSLKLTYESALQQDLKKILGVEEVIMLSTSPMELRLANQKLGNRFIKTLQAMNELDMGEFFNAYAQKTKDPTHA
TSYGVFAASLGIELKKALRHYLYAQTSNMVINCVKSVPLSQNDGQKILLSLQSPFNQLIEKTLELDESHLCTASVQNDIK
AMQHESLYSRLYMS
>Mature_254_residues
MDKGKSVKSTEKSVGMPPKTPKTDNNAHVDNEFLILQVNDAVFPIGSYTHSFGLETYIQQKKVTNKESALEYLKANLSSQ
FLYTEMLSLKLTYESALQQDLKKILGVEEVIMLSTSPMELRLANQKLGNRFIKTLQAMNELDMGEFFNAYAQKTKDPTHA
TSYGVFAASLGIELKKALRHYLYAQTSNMVINCVKSVPLSQNDGQKILLSLQSPFNQLIEKTLELDESHLCTASVQNDIK
AMQHESLYSRLYMS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter [H]

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002639 [H]

Pfam domain/function: PF01730 UreF [H]

EC number: NA

Molecular weight: Translated: 28619; Mature: 28619

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKGKSVKSTEKSVGMPPKTPKTDNNAHVDNEFLILQVNDAVFPIGSYTHSFGLETYIQQ
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCEECCCCHHHHHHHHHHHHH
KKVTNKESALEYLKANLSSQFLYTEMLSLKLTYESALQQDLKKILGVEEVIMLSTSPMEL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCHHE
RLANQKLGNRFIKTLQAMNELDMGEFFNAYAQKTKDPTHATSYGVFAASLGIELKKALRH
EHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
YLYAQTSNMVINCVKSVPLSQNDGQKILLSLQSPFNQLIEKTLELDESHLCTASVQNDIK
HHHHCCCHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AMQHESLYSRLYMS
HHHHHHHHHHHHCC
>Mature Secondary Structure
MDKGKSVKSTEKSVGMPPKTPKTDNNAHVDNEFLILQVNDAVFPIGSYTHSFGLETYIQQ
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCEECCCCHHHHHHHHHHHHH
KKVTNKESALEYLKANLSSQFLYTEMLSLKLTYESALQQDLKKILGVEEVIMLSTSPMEL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCHHE
RLANQKLGNRFIKTLQAMNELDMGEFFNAYAQKTKDPTHATSYGVFAASLGIELKKALRH
EHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
YLYAQTSNMVINCVKSVPLSQNDGQKILLSLQSPFNQLIEKTLELDESHLCTASVQNDIK
HHHHCCCHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AMQHESLYSRLYMS
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]