Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 161723289

GI number: 161723289

Start: 380664

End: 381527

Strand: Direct

Name: thyA [H]

Synonym: MPN320

Alternate gene names: 161723289

Gene position: 380664-381527 (Clockwise)

Preceding gene: 13508058

Following gene: 13508060

Centisome position: 46.63

GC content: 42.01

Gene sequence:

>864_bases
ATGCAGCAGTATCTAGATTTAGCCCGTTATGTTTTAGAGCACGGAAAATACCGTCCAAATCGCACTGATACTGCTGGCAT
TGGTGTCTTTGGTTACCAGATGCACTTTGATATTTCAAAACACTTTCCGCTCTTGACTACGAAGAAGGTGCACTGAAAGT
CGATTGTGCACGAACTTTTGTGGTTCATTAAGGGTGACACCAATATTAAGTACCTAGTCGACAATAAGGTCAACATTTGG
AATGAATGACCTTACGAAAGCTTTAAAAAGTCACCTCACTTTAACGGTGAGAGTCAAAAGGAGTTCATTGAACGGATTCG
ACAAGACGCGAAGTTTGCCCAACAGTTTGGTAACCTCGGACCCGTTTATGGAAAGCAATGGCGTGACTTTAACGGAGTCG
ATCAATTGAAAAAGGTAATTGCGCAAATTAAGGTAAATCCGTTTTCGCGGCGCTTAATTGTGTCTAGTTGAAATCCCAAT
GAGGTGGATCAAATGTTGCTCCCGCCTTGTCATTCACTGTACCAGTTTTACGTACAGGACGGGCAGTTGAGCTGCCAGTT
GTACCAGCGCAGTGGTGATATTTTTCTAGGTATCCCCTTCAACATTGCGTCCTACTCTCTTCTGGTGTACTTAGTTGCTA
AGGAAACTAACCTAAAACCAGGGAGCTTTGTCCACACCATTGGCGATGCCCACATTTACGAAAATCACTTGGAACAAATT
AAGTTGCAATTAACACGTCAACCCAAACCATTGCCCAAGGTAGTTTTAAAGAGTGATAAATCAATTTTTGATTATCAATT
CGATGATATTGAACTGGTGGATTACGACCACCACCCTACAATTAAAGGAGAGGTAGCAGTTTAA

Upstream 100 bases:

>100_bases
AAGTTATTGCAAAAACTCTTTTAAAAAAGTTATTCGCTTATTTTGCAAGCTTAAAAAAAGATTTTTATACAATGCTACAC
CATCGAAGTTTAGGAGAGTT

Downstream 100 bases:

>100_bases
GATGGTAAAAGCAATTTGAGCAATGGATCAAAATGGGTTAATTGGTAACGGTAACAGTTTGCCCTGAAGGATTAAAGCGG
AGTTACAGCACTTTCGCCAG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MQQYLDLARYVLEHGKYRPNRTDTAGIGVFGYQMHFDISKHFPLLTTKKVHWKSIVHELLWFIKGDTNIKYLVDNKVNIW
NEWPYESFKKSPHFNGESQKEFIERIRQDAKFAQQFGNLGPVYGKQWRDFNGVDQLKKVIAQIKVNPFSRRLIVSSWNPN
EVDQMLLPPCHSLYQFYVQDGQLSCQLYQRSGDIFLGIPFNIASYSLLVYLVAKETNLKPGSFVHTIGDAHIYENHLEQI
KLQLTRQPKPLPKVVLKSDKSIFDYQFDDIELVDYDHHPTIKGEVAV

Sequences:

>Translated_287_residues
MQQYLDLARYVLEHGKYRPNRTDTAGIGVFGYQMHFDISKHFPLLTTKKVH*KSIVHELLWFIKGDTNIKYLVDNKVNIW
NE*PYESFKKSPHFNGESQKEFIERIRQDAKFAQQFGNLGPVYGKQWRDFNGVDQLKKVIAQIKVNPFSRRLIVSS*NPN
EVDQMLLPPCHSLYQFYVQDGQLSCQLYQRSGDIFLGIPFNIASYSLLVYLVAKETNLKPGSFVHTIGDAHIYENHLEQI
KLQLTRQPKPLPKVVLKSDKSIFDYQFDDIELVDYDHHPTIKGEVAV
>Mature_287_residues
MQQYLDLARYVLEHGKYRPNRTDTAGIGVFGYQMHFDISKHFPLLTTKKVH*KSIVHELLWFIKGDTNIKYLVDNKVNIW
NE*PYESFKKSPHFNGESQKEFIERIRQDAKFAQQFGNLGPVYGKQWRDFNGVDQLKKVIAQIKVNPFSRRLIVSS*NPN
EVDQMLLPPCHSLYQFYVQDGQLSCQLYQRSGDIFLGIPFNIASYSLLVYLVAKETNLKPGSFVHTIGDAHIYENHLEQI
KLQLTRQPKPLPKVVLKSDKSIFDYQFDDIELVDYDHHPTIKGEVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=298, Percent_Identity=46.6442953020134, Blast_Score=264, Evalue=7e-71,
Organism=Escherichia coli, GI1789191, Length=292, Percent_Identity=49.3150684931507, Blast_Score=290, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI71993377, Length=299, Percent_Identity=45.1505016722408, Blast_Score=251, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6324648, Length=307, Percent_Identity=36.4820846905537, Blast_Score=199, Evalue=6e-52,
Organism=Drosophila melanogaster, GI17137556, Length=299, Percent_Identity=45.819397993311, Blast_Score=271, Evalue=4e-73,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32979; Mature: 32979

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYLDLARYVLEHGKYRPNRTDTAGIGVFGYQMHFDISKHFPLLTTKKVHKSIVHELLW
CHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHH
FIKGDTNIKYLVDNKVNIWNEPYESFKKSPHFNGESQKEFIERIRQDAKFAQQFGNLGPV
HHCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
YGKQWRDFNGVDQLKKVIAQIKVNPFSRRLIVSSNPNEVDQMLLPPCHSLYQFYVQDGQL
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHCCCCE
SCQLYQRSGDIFLGIPFNIASYSLLVYLVAKETNLKPGSFVHTIGDAHIYENHLEQIKLQ
EEEEEECCCCEEEECCCCHHHHHHHEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHH
LTRQPKPLPKVVLKSDKSIFDYQFDDIELVDYDHHPTIKGEVAV
HCCCCCCCHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MQQYLDLARYVLEHGKYRPNRTDTAGIGVFGYQMHFDISKHFPLLTTKKVHKSIVHELLW
CHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHH
FIKGDTNIKYLVDNKVNIWNEPYESFKKSPHFNGESQKEFIERIRQDAKFAQQFGNLGPV
HHCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
YGKQWRDFNGVDQLKKVIAQIKVNPFSRRLIVSSNPNEVDQMLLPPCHSLYQFYVQDGQL
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHCCCCE
SCQLYQRSGDIFLGIPFNIASYSLLVYLVAKETNLKPGSFVHTIGDAHIYENHLEQIKLQ
EEEEEECCCCEEEECCCCHHHHHHHEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHH
LTRQPKPLPKVVLKSDKSIFDYQFDDIELVDYDHHPTIKGEVAV
HCCCCCCCHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633; 7984111 [H]