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The map label for this gene is gidA

Identifier: 13508296

GI number: 13508296

Start: 677332

End: 679170

Strand: Direct

Name: gidA

Synonym: MPN557

Alternate gene names: 13508296

Gene position: 677332-679170 (Clockwise)

Preceding gene: 13508283

Following gene: 13508297

Centisome position: 82.97

GC content: 41.71

Gene sequence:

>1839_bases
ATGTCTTTTACACTAACAGTTATTGGTGGCGGTCATGCCGGATTAGAGGCCGCATTTATTGCTAGTAAACTAGGGCTAAA
AGTTAACCTCTTAGTACTAGATCCCAACCATGTGGGGTCGTGTCCCTGTAACCCGGCCATAGGAGGTCCTGCTAAAGGTA
TAGTAACCCGGGAAATCGATGTTTTAGGAGGGATGCAGGGTAAGGCCGCTGATGCCACTGCTTTACAATACAAGCTGCTC
AACAGCTCGAAAGGTCCAGCAGTGCAAGCAATCCGCGCTCAAATAGACAAAATTGCTTATCAGAAGTGGTTTCGCCAGCA
AATTGACCAAACGCCTAACATTGAGTTAATTGCTGGGGAAGCCGTTGATATCCTTGAAAGTAACGGTAAAGTTAAAGGTG
TCGTGTTAGCTGATGGTAGTGAACTGGCATCAGATGCTGTTATTGTGACTACGGGAACTTATCTAAAGGCCAAAACTTAC
TGTGGTAGCTTAAGCAAAGAAGAAGGTCCTGACCGCGCTAAGCGCAGTGAATACCTTTCCACTAACTTAATTAAGCGGGG
TTTTAAAACGTTACGACTAAAAACTGGGACACCACCACGGATCCTACGCGAATCACTCGACTTTAGCCAAATGGCTGTGG
AAGCTAACACTACCCCTCATTTAGCCTTTAGCTTTACTACTAAGAATTATTTACCACTAGAACAGCAAGTGATCTGTCAC
TTAATCCATACCAATCCCCAGATCCATCAGTTAATTCTGGCCAACTTAAAACAGTCCGCTGTCTTTAACGGTAGTATTAA
AGCAAACGGACCACTTTACTGTCCGAGTATTGAAGACAAGGTATTTCGCTTTCAAGACAAAGAACGGCACCAAATCTTTG
TCGAACCTGAATCACTTAGTTTAGAAACGGTTTACCTAGCAGGGTTTTCCACTTCCTTTCCCCCAGAGGTGCAAGAGCAC
ATTGTCCGTTTACTCCCTGGGTTTAAAAATGCCCGGTTTCAAAAGTATGGCTATGCCATTGAATATGATGCCTTTAGTTC
GATACAACTCAAATCAACACTAGAAACTAAGTTAATTCAAAATCTGTATTTTGCCGGACAGATTAATGGTACCAGTGGGT
ATGAGGAAGCAGCTGGCCAGGGCTTAATCGCTGGGATTAACGCCGCTTTAAAGCTACAGCGTAAACCCGAATTTGTGTTA
CAGCGCAATGAGGCATACCTTGGTGTCATGATTAACGACCTGGTTACTAAGGAAATTAGTGATCCCTACCGCTTGTTAAC
TTCGCGCGCTGAACACCGTTTGTGGTTGCGCAATGATAACTTACAAGAACGTCTAATTGAAAAGAGCCGAGCCTTAGGTT
TAGTGGAAGCAGATGTGTATGCCAATTACTTGGAACAACAACAAAAGAAAAAACAATTAATTGATTATTTGCAAACCACT
ACCGTTGGGCAAATTGCTGCCTTGAAATTAAACTTTAAAAACACGGCCCAAACGCTGTTTGACTTTACCAAGCGTGCTGA
AATTAAGCTGGTTGATTTAGTACAACTACTCCCCAAGCGTTTTGACTTGGATGTACAAAGTTTAAACCAGATTGACATTG
ACATTAAGTACGCTGGTTACATTAAGAAGAGTGAAAAGTACTTCAAGAGTTTAAACAACCTCAGTAGTGTCAAAATTCCC
TTAAAGTTAAATTACCACAAAGTACCTAACTTAGCGAGTGAAGCAATTGTTAAACTCTCCAAAATTAGACCAACTGATCT
CAGTGTAGCTAGTCAAGTAGCGGGCATTAACTTTAACGATATTTTAGCGATTAAACATTTTTTAGATAACCATGAATAA

Upstream 100 bases:

>100_bases
ACTCTTTGTGCTCATCAAATTCTAGATTCAAAGCGTTAAGACATTCTTGAAGATCTGTGTTAATGAAAAGCATTACTTAA
ATTATATTTAACCTTGCAAA

Downstream 100 bases:

>100_bases
CAGTAAATTGAAACAGTATGTTCAACTAGTACAAACAGCCAACCAAAACTTTAACCTCACCGGTCTTAAAACTGAAGGCG
AAATTTACGAACACTTAGTC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MSFTLTVIGGGHAGLEAAFIASKLGLKVNLLVLDPNHVGSCPCNPAIGGPAKGIVTREIDVLGGMQGKAADATALQYKLL
NSSKGPAVQAIRAQIDKIAYQKWFRQQIDQTPNIELIAGEAVDILESNGKVKGVVLADGSELASDAVIVTTGTYLKAKTY
CGSLSKEEGPDRAKRSEYLSTNLIKRGFKTLRLKTGTPPRILRESLDFSQMAVEANTTPHLAFSFTTKNYLPLEQQVICH
LIHTNPQIHQLILANLKQSAVFNGSIKANGPLYCPSIEDKVFRFQDKERHQIFVEPESLSLETVYLAGFSTSFPPEVQEH
IVRLLPGFKNARFQKYGYAIEYDAFSSIQLKSTLETKLIQNLYFAGQINGTSGYEEAAGQGLIAGINAALKLQRKPEFVL
QRNEAYLGVMINDLVTKEISDPYRLLTSRAEHRLWLRNDNLQERLIEKSRALGLVEADVYANYLEQQQKKKQLIDYLQTT
TVGQIAALKLNFKNTAQTLFDFTKRAEIKLVDLVQLLPKRFDLDVQSLNQIDIDIKYAGYIKKSEKYFKSLNNLSSVKIP
LKLNYHKVPNLASEAIVKLSKIRPTDLSVASQVAGINFNDILAIKHFLDNHE

Sequences:

>Translated_612_residues
MSFTLTVIGGGHAGLEAAFIASKLGLKVNLLVLDPNHVGSCPCNPAIGGPAKGIVTREIDVLGGMQGKAADATALQYKLL
NSSKGPAVQAIRAQIDKIAYQKWFRQQIDQTPNIELIAGEAVDILESNGKVKGVVLADGSELASDAVIVTTGTYLKAKTY
CGSLSKEEGPDRAKRSEYLSTNLIKRGFKTLRLKTGTPPRILRESLDFSQMAVEANTTPHLAFSFTTKNYLPLEQQVICH
LIHTNPQIHQLILANLKQSAVFNGSIKANGPLYCPSIEDKVFRFQDKERHQIFVEPESLSLETVYLAGFSTSFPPEVQEH
IVRLLPGFKNARFQKYGYAIEYDAFSSIQLKSTLETKLIQNLYFAGQINGTSGYEEAAGQGLIAGINAALKLQRKPEFVL
QRNEAYLGVMINDLVTKEISDPYRLLTSRAEHRLWLRNDNLQERLIEKSRALGLVEADVYANYLEQQQKKKQLIDYLQTT
TVGQIAALKLNFKNTAQTLFDFTKRAEIKLVDLVQLLPKRFDLDVQSLNQIDIDIKYAGYIKKSEKYFKSLNNLSSVKIP
LKLNYHKVPNLASEAIVKLSKIRPTDLSVASQVAGINFNDILAIKHFLDNHE
>Mature_611_residues
SFTLTVIGGGHAGLEAAFIASKLGLKVNLLVLDPNHVGSCPCNPAIGGPAKGIVTREIDVLGGMQGKAADATALQYKLLN
SSKGPAVQAIRAQIDKIAYQKWFRQQIDQTPNIELIAGEAVDILESNGKVKGVVLADGSELASDAVIVTTGTYLKAKTYC
GSLSKEEGPDRAKRSEYLSTNLIKRGFKTLRLKTGTPPRILRESLDFSQMAVEANTTPHLAFSFTTKNYLPLEQQVICHL
IHTNPQIHQLILANLKQSAVFNGSIKANGPLYCPSIEDKVFRFQDKERHQIFVEPESLSLETVYLAGFSTSFPPEVQEHI
VRLLPGFKNARFQKYGYAIEYDAFSSIQLKSTLETKLIQNLYFAGQINGTSGYEEAAGQGLIAGINAALKLQRKPEFVLQ
RNEAYLGVMINDLVTKEISDPYRLLTSRAEHRLWLRNDNLQERLIEKSRALGLVEADVYANYLEQQQKKKQLIDYLQTTT
VGQIAALKLNFKNTAQTLFDFTKRAEIKLVDLVQLLPKRFDLDVQSLNQIDIDIKYAGYIKKSEKYFKSLNNLSSVKIPL
KLNYHKVPNLASEAIVKLSKIRPTDLSVASQVAGINFNDILAIKHFLDNHE

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=635, Percent_Identity=36.3779527559055, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI19882217, Length=660, Percent_Identity=35, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI183227703, Length=675, Percent_Identity=34.2222222222222, Blast_Score=357, Evalue=1e-98,
Organism=Escherichia coli, GI2367273, Length=604, Percent_Identity=41.7218543046358, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17534255, Length=614, Percent_Identity=35.1791530944625, Blast_Score=392, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321202, Length=614, Percent_Identity=37.4592833876222, Blast_Score=403, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24658174, Length=631, Percent_Identity=35.4992076069731, Blast_Score=352, Evalue=5e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_MYCPN (P75221)

Other databases:

- EMBL:   U00089
- PIR:   S73611
- RefSeq:   NP_110246.1
- ProteinModelPortal:   P75221
- SMR:   P75221
- IntAct:   P75221
- GeneID:   877193
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN557
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   MPNE272634:MPN557-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 68054; Mature: 67923

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTLTVIGGGHAGLEAAFIASKLGLKVNLLVLDPNHVGSCPCNPAIGGPAKGIVTREID
CEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHH
VLGGMQGKAADATALQYKLLNSSKGPAVQAIRAQIDKIAYQKWFRQQIDQTPNIELIAGE
HHCCCCCCCCCHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
AVDILESNGKVKGVVLADGSELASDAVIVTTGTYLKAKTYCGSLSKEEGPDRAKRSEYLS
HHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCEEEEHHHHCCCCCCCCCCHHHHHHHHH
TNLIKRGFKTLRLKTGTPPRILRESLDFSQMAVEANTTPHLAFSFTTKNYLPLEQQVICH
HHHHHCCCCEEEEECCCCHHHHHHCCCHHHEEEECCCCCEEEEEEECCCCCCCHHHHHHH
LIHTNPQIHQLILANLKQSAVFNGSIKANGPLYCPSIEDKVFRFQDKERHQIFVEPESLS
HHCCCCHHHHHHHHHHHHHHEECCEEECCCCEECCCCHHHHHHCCCCCCCEEEECCCCCC
LETVYLAGFSTSFPPEVQEHIVRLLPGFKNARFQKYGYAIEYDAFSSIQLKSTLETKLIQ
EEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHCCEEEEECCCCCEEEHHHHHHHHHH
NLYFAGQINGTSGYEEAAGQGLIAGINAALKLQRKPEFVLQRNEAYLGVMINDLVTKEIS
HHEEEEEECCCCCCHHHCCCCEEEECCEEEEECCCCHHHEECCCCEEEEHHHHHHHHHCC
DPYRLLTSRAEHRLWLRNDNLQERLIEKSRALGLVEADVYANYLEQQQKKKQLIDYLQTT
CHHHHHHHHHHCEEEEECCCHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
TVGQIAALKLNFKNTAQTLFDFTKRAEIKLVDLVQLLPKRFDLDVQSLNQIDIDIKYAGY
CCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCEEEEEEEEECE
IKKSEKYFKSLNNLSSVKIPLKLNYHKVPNLASEAIVKLSKIRPTDLSVASQVAGINFND
EHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCHHH
ILAIKHFLDNHE
HHHHHHHHCCCC
>Mature Secondary Structure 
SFTLTVIGGGHAGLEAAFIASKLGLKVNLLVLDPNHVGSCPCNPAIGGPAKGIVTREID
EEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHH
VLGGMQGKAADATALQYKLLNSSKGPAVQAIRAQIDKIAYQKWFRQQIDQTPNIELIAGE
HHCCCCCCCCCHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
AVDILESNGKVKGVVLADGSELASDAVIVTTGTYLKAKTYCGSLSKEEGPDRAKRSEYLS
HHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCEEEEHHHHCCCCCCCCCCHHHHHHHHH
TNLIKRGFKTLRLKTGTPPRILRESLDFSQMAVEANTTPHLAFSFTTKNYLPLEQQVICH
HHHHHCCCCEEEEECCCCHHHHHHCCCHHHEEEECCCCCEEEEEEECCCCCCCHHHHHHH
LIHTNPQIHQLILANLKQSAVFNGSIKANGPLYCPSIEDKVFRFQDKERHQIFVEPESLS
HHCCCCHHHHHHHHHHHHHHEECCEEECCCCEECCCCHHHHHHCCCCCCCEEEECCCCCC
LETVYLAGFSTSFPPEVQEHIVRLLPGFKNARFQKYGYAIEYDAFSSIQLKSTLETKLIQ
EEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHCCEEEEECCCCCEEEHHHHHHHHHH
NLYFAGQINGTSGYEEAAGQGLIAGINAALKLQRKPEFVLQRNEAYLGVMINDLVTKEIS
HHEEEEEECCCCCCHHHCCCCEEEECCEEEEECCCCHHHEECCCCEEEEHHHHHHHHHCC
DPYRLLTSRAEHRLWLRNDNLQERLIEKSRALGLVEADVYANYLEQQQKKKQLIDYLQTT
CHHHHHHHHHHCEEEEECCCHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
TVGQIAALKLNFKNTAQTLFDFTKRAEIKLVDLVQLLPKRFDLDVQSLNQIDIDIKYAGY
CCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCEEEEEEEEECE
IKKSEKYFKSLNNLSSVKIPLKLNYHKVPNLASEAIVKLSKIRPTDLSVASQVAGINFND
EHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCHHH
ILAIKHFLDNHE
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633