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The map label for this gene is ygl3 [C]

Identifier: 13508260

GI number: 13508260

Start: 642760

End: 643260

Strand: Direct

Name: ygl3 [C]

Synonym: MPN521

Alternate gene names: 13508260

Gene position: 642760-643260 (Clockwise)

Preceding gene: 13508246

Following gene: 13508261

Centisome position: 78.73

GC content: 38.32

Gene sequence:

>501_bases
ATGTATAAATCAGCGATTAATATCGTCTTATTTTGCCCAGAAATTCCTAATAACACTGGGAACATTGTTCGGAGCTGTAC
CGCTTTTAAAGCTAACTTGCATCTGATTAAGCCCTATGGCTTTTTTCTCAATGATAAGCGGATGGTGCGTGCTGGGTTAA
ATTGTTGGGATAAGGTGCAAATGTTTGAACACAAATCCTGGGAACACTTCATTGAAACAACACCACCAAATAAAGCAATT
TGGTTGTTGACAAAGAGTGGTACTACTACCCCAGACCAAATTAACATGCAAACAGATAACAGGGAACAACTGTACTTTGT
TTTTGGTCAAGAAACCAAGGGATTACCGCAAACACTCATGGAACAATACTCGCAGAACCAGGTACGGATTCCGATTTGAA
ACCCTGTAAGAAGCATTAACTTGTCCAATACAGTAGCTTGTGTACTGTATGAGTATGCTAAACAAAATCATTACTTTAAT
TTAGATAAACAGTGCGCTTAA

Upstream 100 bases:

>100_bases
ATTTGCCCTGCATTTCAAATGCAGTTTGGGGCATTAACAGTGTCTTTTTTAAGTTCATTAAGCATAGTAATTTTATTAAT
ATTTAACTAACTTCTATCTG

Downstream 100 bases:

>100_bases
GAAAAGTTAAAGATGCTCTTGTAAGGGTACAAGACAGCCCTTACTTTAGTAACCCAGAACAACTGTCAACTATTGAAAGT
GAATCGCTCTTCGTGGAAAT

Product: rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MYKSAINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNCWDKVQMFEHKSWEHFIETTPPNKAI
WLLTKSGTTTPDQINMQTDNREQLYFVFGQETKGLPQTLMEQYSQNQVRIPIWNPVRSINLSNTVACVLYEYAKQNHYFN
LDKQCA

Sequences:

>Translated_166_residues
MYKSAINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNCWDKVQMFEHKSWEHFIETTPPNKAI
WLLTKSGTTTPDQINMQTDNREQLYFVFGQETKGLPQTLMEQYSQNQVRIPI*NPVRSINLSNTVACVLYEYAKQNHYFN
LDKQCA
>Mature_166_residues
MYKSAINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNCWDKVQMFEHKSWEHFIETTPPNKAI
WLLTKSGTTTPDQINMQTDNREQLYFVFGQETKGLPQTLMEQYSQNQVRIPI*NPVRSINLSNTVACVLYEYAKQNHYFN
LDKQCA

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=154, Percent_Identity=39.6103896103896, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19152; Mature: 19152

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKSAINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNCWDKVQ
CCCCEEEEEEECCCCCCCCCHHHHHHEEEECCEEEECCCCEEECCCHHHHHCCCHHHHHH
MFEHKSWEHFIETTPPNKAIWLLTKSGTTTPDQINMQTDNREQLYFVFGQETKGLPQTLM
HHHCCCCHHHEECCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCCCCHHHHH
EQYSQNQVRIPINPVRSINLSNTVACVLYEYAKQNHYFNLDKQCA
HHHCCCEEEEECCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MYKSAINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNCWDKVQ
CCCCEEEEEEECCCCCCCCCHHHHHHEEEECCEEEECCCCEEECCCHHHHHCCCHHHHHH
MFEHKSWEHFIETTPPNKAIWLLTKSGTTTPDQINMQTDNREQLYFVFGQETKGLPQTLM
HHHCCCCHHHEECCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCCCCHHHHH
EQYSQNQVRIPINPVRSINLSNTVACVLYEYAKQNHYFNLDKQCA
HHHCCCEEEEECCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]