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The map label for this gene is nifS [H]

Identifier: 13508226

GI number: 13508226

Start: 590569

End: 591795

Strand: Direct

Name: nifS [H]

Synonym: MPN487

Alternate gene names: 13508226

Gene position: 590569-591795 (Clockwise)

Preceding gene: 13508225

Following gene: 13508227

Centisome position: 72.34

GC content: 41.08

Gene sequence:

>1227_bases
ATGACCAAAACTAAGTTTAATCCGTACCAATTTCGCAAACAATTTAAGTGGTTTAAAAACAACCCCCAATGGGTTAACTT
TGACAACGCTGCTACCTCAATTGCGCTAGACACCGTTAGTCAAGCTTGCAAGGAATACTATGAGCTTTTTAGTGTCAACC
CCCACAATAAAACCCCTGATCTCAACAACCAGATTATTGCCATTATTGCTGAAACCCGTCAACTAGTAGCGGACTGGTTT
AATGTCACTGCACTTGAAATTATCTTTACAAGTTCAGCCACGGAGTCCATTAATTTGTTTGCCCATGGCTTAAAGCCCTG
GATTAAACCCGGTGATGAAATTGTCTTAAAGGGTGATGAACACTCGGCTAACGTTTTGCCCTGAGTTGCACTAGCTAAAC
AAACAAAGGCCAGACTAGTATGGGTCGAAAAGCAAGCTAACCAATCCTTAGAAGACACTTTTAAGTCATTGATTAACCCG
AAAACTAAAGTAGTAGCCATTACCGCTACTTCCAATTTGTTTGGCAATTCAATTGACTTTGCTCAAATTGCCGATTACCT
CAAACAAGTCAATCCTAAAGCTTTTGTAGCCGTGGATGCAGTGCAAACGGTGCAGCACAAGCAAATTGACATTGCCAAAA
CCCAGATCGACTTCCTAGCCTTTTCTACTCATAAGTTTTACGGTCCCACTGGACTGGGGGTAGCTTACATTAAAAAAAGC
TTACAACCCCAATTACAACCCCTCAAACTCGGGGGTGATATCTTTACCCAGATTGACCCGGACAATACAATTCACTTTAA
AAGTACACCCCTAAAGTTTGAAGCCGGTACCCCCAACATTATGGCCATCTATGCCTTAAACAAGCTGTTGCGCTTCTTTA
AACAAAAGTTTAATTTTGCCCAAATGATGGCTTATGGTCACCAACTTAAACAACAAGCTTACGAACTGTTAAACAGTAAT
CCGCAAATTGTGCTGGCCAACCACGACCAGGATGTCCCGATTTTTTCCTTTAAACACCGCCAATTGGCTACCATTGATCT
AGCCACTTTCCTAAACATCAATAAGATCATGGTACGCCAGGGCTCCATTTGTGTGGGACGTTACCGCAATAAGGATTACT
TTGTGAGAGTTTCGTTAATGCACTATAACACAGTGAAGGAGTTGCAGTATTTAGCTAAACTCCTAGCAACTGATACCAAG
ACAATTATTAAAAACGTAATTAAGTAA

Upstream 100 bases:

>100_bases
AGAGCAAAAGCTGTAAGCCACTTTATAATTAAGTAAATTATAGCAACGCTGTCAAAGCGTACTTTTACCATTGATTATTT
CCTTAAAAAGACTTGTTTAA

Downstream 100 bases:

>100_bases
CTATGGATCGCAAAATAAGGGAGCAAATTATTGCCATTTACAGCAACTTACAACACAAACAGGACTTAACTCAGTTTGCC
CATTGTTTAACCACTAAGGC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTKTKFNPYQFRKQFKWFKNNPQWVNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPDLNNQIIAIIAETRQLVADWF
NVTALEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDEHSANVLPWVALAKQTKARLVWVEKQANQSLEDTFKSLINP
KTKVVAITATSNLFGNSIDFAQIADYLKQVNPKAFVAVDAVQTVQHKQIDIAKTQIDFLAFSTHKFYGPTGLGVAYIKKS
LQPQLQPLKLGGDIFTQIDPDNTIHFKSTPLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQMMAYGHQLKQQAYELLNSN
PQIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQGSICVGRYRNKDYFVRVSLMHYNTVKELQYLAKLLATDTK
TIIKNVIK

Sequences:

>Translated_408_residues
MTKTKFNPYQFRKQFKWFKNNPQWVNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPDLNNQIIAIIAETRQLVADWF
NVTALEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDEHSANVLP*VALAKQTKARLVWVEKQANQSLEDTFKSLINP
KTKVVAITATSNLFGNSIDFAQIADYLKQVNPKAFVAVDAVQTVQHKQIDIAKTQIDFLAFSTHKFYGPTGLGVAYIKKS
LQPQLQPLKLGGDIFTQIDPDNTIHFKSTPLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQMMAYGHQLKQQAYELLNSN
PQIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQGSICVGRYRNKDYFVRVSLMHYNTVKELQYLAKLLATDTK
TIIKNVIK
>Mature_407_residues
TKTKFNPYQFRKQFKWFKNNPQWVNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPDLNNQIIAIIAETRQLVADWFN
VTALEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDEHSANVLP*VALAKQTKARLVWVEKQANQSLEDTFKSLINPK
TKVVAITATSNLFGNSIDFAQIADYLKQVNPKAFVAVDAVQTVQHKQIDIAKTQIDFLAFSTHKFYGPTGLGVAYIKKSL
QPQLQPLKLGGDIFTQIDPDNTIHFKSTPLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQMMAYGHQLKQQAYELLNSNP
QIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQGSICVGRYRNKDYFVRVSLMHYNTVKELQYLAKLLATDTKT
IIKNVIK

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=202, Percent_Identity=27.7227722772277, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI156713448, Length=297, Percent_Identity=24.9158249158249, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789175, Length=392, Percent_Identity=30.3571428571429, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1787970, Length=378, Percent_Identity=29.1005291005291, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI48994898, Length=201, Percent_Identity=26.865671641791, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=236, Percent_Identity=25.8474576271186, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319831, Length=210, Percent_Identity=30.4761904761905, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI20129463, Length=256, Percent_Identity=26.953125, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 46279; Mature: 46148

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTKFNPYQFRKQFKWFKNNPQWVNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPD
CCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LNNQIIAIIAETRQLVADWFNVTALEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDE
CCCCEEEEEHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHCCCHHCCCCCEEEEECCC
HSANVLPVALAKQTKARLVWVEKQANQSLEDTFKSLINPKTKVVAITATSNLFGNSIDFA
CCCCEEEEEHHCCCCEEEEEEECHHCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHH
QIADYLKQVNPKAFVAVDAVQTVQHKQIDIAKTQIDFLAFSTHKFYGPTGLGVAYIKKSL
HHHHHHHHCCCCEEEEEHHHHHHHHCCCCHHHHEEEEEEEECCCCCCCCCCHHHHHHHHC
QPQLQPLKLGGDIFTQIDPDNTIHFKSTPLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQ
CCCCCEEEECCCEEEEECCCCEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHCCHHH
MMAYGHQLKQQAYELLNSNPQIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQG
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCEEEEEHHHHHHCCCCEEEECC
SICVGRYRNKDYFVRVSLMHYNTVKELQYLAKLLATDTKTIIKNVIK
CEEEEEECCCCEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
TKTKFNPYQFRKQFKWFKNNPQWVNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPD
CCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LNNQIIAIIAETRQLVADWFNVTALEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDE
CCCCEEEEEHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHCCCHHCCCCCEEEEECCC
HSANVLPVALAKQTKARLVWVEKQANQSLEDTFKSLINPKTKVVAITATSNLFGNSIDFA
CCCCEEEEEHHCCCCEEEEEEECHHCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHH
QIADYLKQVNPKAFVAVDAVQTVQHKQIDIAKTQIDFLAFSTHKFYGPTGLGVAYIKKSL
HHHHHHHHCCCCEEEEEHHHHHHHHCCCCHHHHEEEEEEEECCCCCCCCCCHHHHHHHHC
QPQLQPLKLGGDIFTQIDPDNTIHFKSTPLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQ
CCCCCEEEECCCEEEEECCCCEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHCCHHH
MMAYGHQLKQQAYELLNSNPQIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQG
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCEEEEEHHHHHHCCCCEEEECC
SICVGRYRNKDYFVRVSLMHYNTVKELQYLAKLLATDTKTIIKNVIK
CEEEEEECCCCEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]