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The map label for this gene is nfo [H]

Identifier: 13508067

GI number: 13508067

Start: 386734

End: 387594

Strand: Direct

Name: nfo [H]

Synonym: MPN328

Alternate gene names: 13508067

Gene position: 386734-387594 (Clockwise)

Preceding gene: 13508066

Following gene: 13508068

Centisome position: 47.37

GC content: 42.16

Gene sequence:

>861_bases
ATGCCTAAACTCCTAGGAAGCTTTATTTCGTTTAAATCACCCCATTATCTAGTGGGGAGTGTTCGTGACGCTGTTAGCAT
TAAAGCACAAGCGTTCATGATCTTTTTGGGCGCACCACACACGGCACTACGAGTCGATCCTAATCGTATGCAAATTGACG
AGGGTCACACATTGATGGAACAACATAACCTCAGTAAGTCTGGGATGGTTGTCCATGCTCCTTACATTATTAACTGTGCC
TCCAAAGATCCGGTAAAGCAAACCTTTGCGATTGACGTTCTGACAAGGGAGGTTAAACTGTGTCATGCCGTGGGTGCTAA
ACTAATCGTTCTCCACCCCGGTTCAGCGGTTGAGCAAACTCAAACCCAAGCGTTAGATCACCTAATCAAGGTCTTAAATA
CAGTGATTGCCAACACTAAAGAAGTCATTATTTGTCTCGAAACGATGGCTGGTAAAGGTAATGAAATTGGCCGTGATTTA
GACCAGTTAAAGTATGTGATTAACCACATTGAACAACAAGAGCGGATTGGTGTTTGTCTAGATACCTGTCACTTCCATGA
CAGTGGTAATGACTTTAACAATACTGCCGAAATTATGGAAACGATTGACACTAAACTAGGCTTTGAGTTTTTAAAGGTCA
TCCACTTAAACGAGTCCAAAAACGTTTGTGGTTCCAAAAAAGACCGTCATGCCAATCTAGGTGAGGGGATGATTGGGTTT
GATAATTTAATGCGCTTTATTGCCCAACCGCAAATAAAGCAAATACCGATTGTTTTAGAAACCCCATCTGACAAGCATAA
TTATCCCGCTGTATATGGTGCTGAAATCGAAAGGATCCGTGCTTGATTTGGAGCACGATAA

Upstream 100 bases:

>100_bases
GATGACACCTTGTTTGCTTTAAGCGACGGCTTGGTAAAATACCAAAAGTTTGGACCTAAACAGGGCAAGACCCGCGTGAG
TGTGGTCAAACACAAACTAG

Downstream 100 bases:

>100_bases
CCAAACACTAAAGGATTACATTAGTGTGTTTGAACGCAATAAGATGCGTTTAACCCAATCACGCTTAATGTTGCTCCAAT
GCTTAGTGCAACACCGTGAC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MPKLLGSFISFKSPHYLVGSVRDAVSIKAQAFMIFLGAPHTALRVDPNRMQIDEGHTLMEQHNLSKSGMVVHAPYIINCA
SKDPVKQTFAIDVLTREVKLCHAVGAKLIVLHPGSAVEQTQTQALDHLIKVLNTVIANTKEVIICLETMAGKGNEIGRDL
DQLKYVINHIEQQERIGVCLDTCHFHDSGNDFNNTAEIMETIDTKLGFEFLKVIHLNESKNVCGSKKDRHANLGEGMIGF
DNLMRFIAQPQIKQIPIVLETPSDKHNYPAVYGAEIERIRAWFGAR

Sequences:

>Translated_286_residues
MPKLLGSFISFKSPHYLVGSVRDAVSIKAQAFMIFLGAPHTALRVDPNRMQIDEGHTLMEQHNLSKSGMVVHAPYIINCA
SKDPVKQTFAIDVLTREVKLCHAVGAKLIVLHPGSAVEQTQTQALDHLIKVLNTVIANTKEVIICLETMAGKGNEIGRDL
DQLKYVINHIEQQERIGVCLDTCHFHDSGNDFNNTAEIMETIDTKLGFEFLKVIHLNESKNVCGSKKDRHANLGEGMIGF
DNLMRFIAQPQIKQIPIVLETPSDKHNYPAVYGAEIERIRA*FGAR
>Mature_285_residues
PKLLGSFISFKSPHYLVGSVRDAVSIKAQAFMIFLGAPHTALRVDPNRMQIDEGHTLMEQHNLSKSGMVVHAPYIINCAS
KDPVKQTFAIDVLTREVKLCHAVGAKLIVLHPGSAVEQTQTQALDHLIKVLNTVIANTKEVIICLETMAGKGNEIGRDLD
QLKYVINHIEQQERIGVCLDTCHFHDSGNDFNNTAEIMETIDTKLGFEFLKVIHLNESKNVCGSKKDRHANLGEGMIGFD
NLMRFIAQPQIKQIPIVLETPSDKHNYPAVYGAEIERIRA*FGAR

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=286, Percent_Identity=30.0699300699301, Blast_Score=135, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17531193, Length=271, Percent_Identity=28.7822878228782, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322735, Length=263, Percent_Identity=35.361216730038, Blast_Score=148, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 31686; Mature: 31555

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLLGSFISFKSPHYLVGSVRDAVSIKAQAFMIFLGAPHTALRVDPNRMQIDEGHTLME
CCHHHHHHHHCCCCCEEHHHHHHHHHHEEEEEEEEEECCCCEEEECCCCEEECCCCHHHH
QHNLSKSGMVVHAPYIINCASKDPVKQTFAIDVLTREVKLCHAVGAKLIVLHPGSAVEQT
HHCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
QTQALDHLIKVLNTVIANTKEVIICLETMAGKGNEIGRDLDQLKYVINHIEQQERIGVCL
HHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHH
DTCHFHDSGNDFNNTAEIMETIDTKLGFEFLKVIHLNESKNVCGSKKDRHANLGEGMIGF
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCH
DNLMRFIAQPQIKQIPIVLETPSDKHNYPAVYGAEIERIRAFGAR
HHHHHHHCCCCHHHCCEEEECCCCCCCCCEEEHHHHHHHHHCCCC
>Mature Secondary Structure 
PKLLGSFISFKSPHYLVGSVRDAVSIKAQAFMIFLGAPHTALRVDPNRMQIDEGHTLME
CHHHHHHHHCCCCCEEHHHHHHHHHHEEEEEEEEEECCCCEEEECCCCEEECCCCHHHH
QHNLSKSGMVVHAPYIINCASKDPVKQTFAIDVLTREVKLCHAVGAKLIVLHPGSAVEQT
HHCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
QTQALDHLIKVLNTVIANTKEVIICLETMAGKGNEIGRDLDQLKYVINHIEQQERIGVCL
HHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHH
DTCHFHDSGNDFNNTAEIMETIDTKLGFEFLKVIHLNESKNVCGSKKDRHANLGEGMIGF
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCH
DNLMRFIAQPQIKQIPIVLETPSDKHNYPAVYGAEIERIRAFGAR
HHHHHHHCCCCHHHCCEEEECCCCCCCCCEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]