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The map label for this gene is nrdE [H]

Identifier: 13508063

GI number: 13508063

Start: 383525

End: 385690

Strand: Direct

Name: nrdE [H]

Synonym: MPN324

Alternate gene names: 13508063

Gene position: 383525-385690 (Clockwise)

Preceding gene: 13508062

Following gene: 13508064

Centisome position: 46.98

GC content: 42.29

Gene sequence:

>2166_bases
ATGTCAGTTAAAGAAAAGATTCCTGCTTTCAACACCCAAGAAGACTTAGAAAGCTATATTTCCTTAAATGCCTACACTAA
GGTGTACGGTGATTTTAAGATGGATTTACACGCTGTGGAGGCTTACATCCAAGAGCATGTCAAACCCAAAACAAAGGTGT
TTCACTCCACCAAGGAACGCTTAGACTTTCTAGTAAAGAATGATTATTACGATGAGAACATCATCAACATGTACAGCTTT
GAGCAGTTTGAGGAAATTACACGTAAGGCTTACGCTTACCGTTTTCGTTATGCTAACTTTATGGGGGCATTTAAGTTTTA
CAACGCTTATGCACTGAAAACCTTTGATGGGAAGTGGTATTTAGAAAACTATGAAGACCGCGTTGTCATGAACGTGCTCT
TTTTAGCCAATGGTAACTATAACAAGGCTTTAAAGTTACTCAAACAAATTATTACCAACCGCTTTCAACCAGCTACCCCA
ACTTTTTTAAATGCGGGACGAAAAAAACGTGGTGAGTTTGTGTCGTGTTACTTACTGCGCATAGAAGACAACATGGAATC
AATTGGTCGTGCCATTACCACTACCCTACAGCTCTCCAAGCGTGATGGTGGGGTGGCGTTATTGCTCACTAACATCCGCG
AATCGGGTGCGCCAATTAAAAAAATAGAAAACCAGTCATCAGGCATTATTCCAATTATGAAGCTGTTGGAAGACTCTTTT
TCTTACGCCAACCAATTAGGGCAAAGACAGGGAGCAGGAGCAGTGTATCTCCATGCCCACCACCCCGATGTCATGCAGTT
TTTAGACACAAAGCGCGAAAACGCTGATGAAAAGATCCGGATTAAGTCGCTGTCTTTGGGTCTAGTTATTCCCGACATTA
CCTTTACTTTAGCTAAAAACAATGAGGAGATGGCCTTGTTTAGTCCGTATGATGTGTATGAGGAGTATGGCAAACCCTTG
TCAGACATTTCAGTCACGGAAATGTATTACGAACTGTTAGCGAACCAACGCATTAAAAAGACCTTTATTAATGCGCGCAA
GTTCTTCCAAACAGTGGCGGAACTGCACTTTGAAAGCGGTTATCCTTACATCTTATTTGACGATACGGTTAACCGGCGCA
ACGCGCACCCCAACCGCATTGTCATGTCTAACCTCTGTAGTGAAATTGTCCAACCCTCCACTCCATCGGAGTTCCACCAC
GATTTGGCTTTTAAAAAGGTTGGTAATGACATTAGCTGTAACTTGGGTAGCTTAAACATTGCCAAAGCAATGGAAAGCGG
ACCGGAGTTCTCGGAGTTGGTTAAACTAGCGATCGAATCACTTGATTTAGTTTCAAGGGTAAGCAATTTAGAAACGGCTC
CATCGATCCAAAAGGGTAATTCCGAAAATCATGCTTTGGGATTAGGGGCAATGAACTTGCACGGTTTTTTAGCAACTAAC
CAAATTTATTACAACTCCCCTGAAGCAATCGACTTTACTAACATCTTTTTCTATACGGTAGCTTACCATGCCTTTAAAGC
ATCCAGTGAGCTAGCTTTAGAGAAGGGTAAGTTTAAAAACTTTGAAAACACCAAGTTTGCTGATGGGAGCTACTTTGATA
AATATATCAAAGTAGAACCAGACTTTTGAACACCCAAAACCGAGCGCGTTAAGGCGTTGTTTCAAAAGTACCAAGTGGAA
ATACCAACACGTGAAAACTGAAAGGAGTTAGCACTCAACATTCAGAAAAACGGTTTGGCCAATTCCCACTTACTGGCAAT
TGCTCCAACGGGTTCAATTTCCTATCTCTCCTCCTGTACTCCTTCACTCCAACCGGTAGTATCCCCGGTAGAAGTGCGCA
AGGAAGGTCGGTTGGGACGGATTTATGTACCGGCTTACCAACTCAATAAAGACAGTTACCCTTTTTATAAAGATGGTGCT
TACGAACTGGGTCCCGAACCAATTATTAACATAGCAGCAGCGGCACAACAACACGTCGATCAAGCGATTTCGCTCACCCT
ATTTATGACTGATAAGGCTACCACTCGTGACCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTTCTTCCATTT
ACTATGTCCGAGTGCGCCAGGAAGTACTAGAGGACAGTGAGGATCACACCATTCAAATGCAGCAGTGTGAGGCCTGTGTG
ATTTAA

Upstream 100 bases:

>100_bases
TTGAACTTTTAGGCACGAAAAACGATGTAGAACAAGTTCAAACCATTATTACCAATTTCTTTGGTAAAGCCAAATAACCG
TTATTAATGATTAGCCAATT

Downstream 100 bases:

>100_bases
TAATACATATAAATACATATAGTAAGTCTAGCCTTAAGCATGACTTTAGCAGGGGTTAATTTGTTATAATTTCACGCTTA
TTTGCGTCTTCTGCGGTTCC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MSVKEKIPAFNTQEDLESYISLNAYTKVYGDFKMDLHAVEAYIQEHVKPKTKVFHSTKERLDFLVKNDYYDENIINMYSF
EQFEEITRKAYAYRFRYANFMGAFKFYNAYALKTFDGKWYLENYEDRVVMNVLFLANGNYNKALKLLKQIITNRFQPATP
TFLNAGRKKRGEFVSCYLLRIEDNMESIGRAITTTLQLSKRDGGVALLLTNIRESGAPIKKIENQSSGIIPIMKLLEDSF
SYANQLGQRQGAGAVYLHAHHPDVMQFLDTKRENADEKIRIKSLSLGLVIPDITFTLAKNNEEMALFSPYDVYEEYGKPL
SDISVTEMYYELLANQRIKKTFINARKFFQTVAELHFESGYPYILFDDTVNRRNAHPNRIVMSNLCSEIVQPSTPSEFHH
DLAFKKVGNDISCNLGSLNIAKAMESGPEFSELVKLAIESLDLVSRVSNLETAPSIQKGNSENHALGLGAMNLHGFLATN
QIYYNSPEAIDFTNIFFYTVAYHAFKASSELALEKGKFKNFENTKFADGSYFDKYIKVEPDFWTPKTERVKALFQKYQVE
IPTRENWKELALNIQKNGLANSHLLAIAPTGSISYLSSCTPSLQPVVSPVEVRKEGRLGRIYVPAYQLNKDSYPFYKDGA
YELGPEPIINIAAAAQQHVDQAISLTLFMTDKATTRDLNKAYIYAFKKGCSSIYYVRVRQEVLEDSEDHTIQMQQCEACV
I

Sequences:

>Translated_721_residues
MSVKEKIPAFNTQEDLESYISLNAYTKVYGDFKMDLHAVEAYIQEHVKPKTKVFHSTKERLDFLVKNDYYDENIINMYSF
EQFEEITRKAYAYRFRYANFMGAFKFYNAYALKTFDGKWYLENYEDRVVMNVLFLANGNYNKALKLLKQIITNRFQPATP
TFLNAGRKKRGEFVSCYLLRIEDNMESIGRAITTTLQLSKRDGGVALLLTNIRESGAPIKKIENQSSGIIPIMKLLEDSF
SYANQLGQRQGAGAVYLHAHHPDVMQFLDTKRENADEKIRIKSLSLGLVIPDITFTLAKNNEEMALFSPYDVYEEYGKPL
SDISVTEMYYELLANQRIKKTFINARKFFQTVAELHFESGYPYILFDDTVNRRNAHPNRIVMSNLCSEIVQPSTPSEFHH
DLAFKKVGNDISCNLGSLNIAKAMESGPEFSELVKLAIESLDLVSRVSNLETAPSIQKGNSENHALGLGAMNLHGFLATN
QIYYNSPEAIDFTNIFFYTVAYHAFKASSELALEKGKFKNFENTKFADGSYFDKYIKVEPDF*TPKTERVKALFQKYQVE
IPTREN*KELALNIQKNGLANSHLLAIAPTGSISYLSSCTPSLQPVVSPVEVRKEGRLGRIYVPAYQLNKDSYPFYKDGA
YELGPEPIINIAAAAQQHVDQAISLTLFMTDKATTRDLNKAYIYAFKKGCSSIYYVRVRQEVLEDSEDHTIQMQQCEACV
I
>Mature_720_residues
SVKEKIPAFNTQEDLESYISLNAYTKVYGDFKMDLHAVEAYIQEHVKPKTKVFHSTKERLDFLVKNDYYDENIINMYSFE
QFEEITRKAYAYRFRYANFMGAFKFYNAYALKTFDGKWYLENYEDRVVMNVLFLANGNYNKALKLLKQIITNRFQPATPT
FLNAGRKKRGEFVSCYLLRIEDNMESIGRAITTTLQLSKRDGGVALLLTNIRESGAPIKKIENQSSGIIPIMKLLEDSFS
YANQLGQRQGAGAVYLHAHHPDVMQFLDTKRENADEKIRIKSLSLGLVIPDITFTLAKNNEEMALFSPYDVYEEYGKPLS
DISVTEMYYELLANQRIKKTFINARKFFQTVAELHFESGYPYILFDDTVNRRNAHPNRIVMSNLCSEIVQPSTPSEFHHD
LAFKKVGNDISCNLGSLNIAKAMESGPEFSELVKLAIESLDLVSRVSNLETAPSIQKGNSENHALGLGAMNLHGFLATNQ
IYYNSPEAIDFTNIFFYTVAYHAFKASSELALEKGKFKNFENTKFADGSYFDKYIKVEPDF*TPKTERVKALFQKYQVEI
PTREN*KELALNIQKNGLANSHLLAIAPTGSISYLSSCTPSLQPVVSPVEVRKEGRLGRIYVPAYQLNKDSYPFYKDGAY
ELGPEPIINIAAAAQQHVDQAISLTLFMTDKATTRDLNKAYIYAFKKGCSSIYYVRVRQEVLEDSEDHTIQMQQCEACVI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=587, Percent_Identity=25.3833049403748, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1789030, Length=715, Percent_Identity=53.8461538461538, Blast_Score=808, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=694, Percent_Identity=26.2247838616715, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17555242, Length=593, Percent_Identity=26.1382799325464, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320914, Length=657, Percent_Identity=26.6362252663623, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6322123, Length=620, Percent_Identity=26.1290322580645, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17352481, Length=644, Percent_Identity=24.6894409937888, Blast_Score=163, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81967; Mature: 81836

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKEKIPAFNTQEDLESYISLNAYTKVYGDFKMDLHAVEAYIQEHVKPKTKVFHSTKER
CCCHHCCCCCCCHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LDFLVKNDYYDENIINMYSFEQFEEITRKAYAYRFRYANFMGAFKFYNAYALKTFDGKWY
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCEEE
LENYEDRVVMNVLFLANGNYNKALKLLKQIITNRFQPATPTFLNAGRKKRGEFVSCYLLR
ECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEE
IEDNMESIGRAITTTLQLSKRDGGVALLLTNIRESGAPIKKIENQSSGIIPIMKLLEDSF
EECCHHHHHHHHHHHEEEECCCCCEEEEEECCHHCCCCHHHHCCCCCCHHHHHHHHHHHH
SYANQLGQRQGAGAVYLHAHHPDVMQFLDTKRENADEKIRIKSLSLGLVIPDITFTLAKN
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECCEEEEEECC
NEEMALFSPYDVYEEYGKPLSDISVTEMYYELLANQRIKKTFINARKFFQTVAELHFESG
CCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
YPYILFDDTVNRRNAHPNRIVMSNLCSEIVQPSTPSEFHHDLAFKKVGNDISCNLGSLNI
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEECCCCCHH
AKAMESGPEFSELVKLAIESLDLVSRVSNLETAPSIQKGNSENHALGLGAMNLHGFLATN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECEEECEEEEEC
QIYYNSPEAIDFTNIFFYTVAYHAFKASSELALEKGKFKNFENTKFADGSYFDKYIKVEP
EEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEECC
DFTPKTERVKALFQKYQVEIPTRENKELALNIQKNGLANSHLLAIAPTGSISYLSSCTPS
CCCCHHHHHHHHHHHHEEECCCCCCCEEEEEEECCCCCCCEEEEEECCCCHHHHHHCCCC
LQPVVSPVEVRKEGRLGRIYVPAYQLNKDSYPFYKDGAYELGPEPIINIAAAAQQHVDQA
HHHCCCHHHHHCCCCEEEEEECEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ISLTLFMTDKATTRDLNKAYIYAFKKGCSSIYYVRVRQEVLEDSEDHTIQMQQCEACVI
EEEEEEEECCCCHHCCCHHHHHHHHHCCCCEEEHEEHHHHHCCCCCCEEEHHHHCCCCC
>Mature Secondary Structure 
SVKEKIPAFNTQEDLESYISLNAYTKVYGDFKMDLHAVEAYIQEHVKPKTKVFHSTKER
CCHHCCCCCCCHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LDFLVKNDYYDENIINMYSFEQFEEITRKAYAYRFRYANFMGAFKFYNAYALKTFDGKWY
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCEEE
LENYEDRVVMNVLFLANGNYNKALKLLKQIITNRFQPATPTFLNAGRKKRGEFVSCYLLR
ECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEE
IEDNMESIGRAITTTLQLSKRDGGVALLLTNIRESGAPIKKIENQSSGIIPIMKLLEDSF
EECCHHHHHHHHHHHEEEECCCCCEEEEEECCHHCCCCHHHHCCCCCCHHHHHHHHHHHH
SYANQLGQRQGAGAVYLHAHHPDVMQFLDTKRENADEKIRIKSLSLGLVIPDITFTLAKN
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECCEEEEEECC
NEEMALFSPYDVYEEYGKPLSDISVTEMYYELLANQRIKKTFINARKFFQTVAELHFESG
CCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
YPYILFDDTVNRRNAHPNRIVMSNLCSEIVQPSTPSEFHHDLAFKKVGNDISCNLGSLNI
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEECCCCCHH
AKAMESGPEFSELVKLAIESLDLVSRVSNLETAPSIQKGNSENHALGLGAMNLHGFLATN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECEEECEEEEEC
QIYYNSPEAIDFTNIFFYTVAYHAFKASSELALEKGKFKNFENTKFADGSYFDKYIKVEP
EEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEECC
DFTPKTERVKALFQKYQVEIPTRENKELALNIQKNGLANSHLLAIAPTGSISYLSSCTPS
CCCCHHHHHHHHHHHHEEECCCCCCCEEEEEEECCCCCCCEEEEEECCCCHHHHHHCCCC
LQPVVSPVEVRKEGRLGRIYVPAYQLNKDSYPFYKDGAYELGPEPIINIAAAAQQHVDQA
HHHCCCHHHHHCCCCEEEEEECEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ISLTLFMTDKATTRDLNKAYIYAFKKGCSSIYYVRVRQEVLEDSEDHTIQMQQCEACVI
EEEEEEEECCCCHHCCCHHHHHHHHHCCCCEEEHEEHHHHHCCCCCCEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]