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The map label for this gene is hsdS1B

Identifier: 13508028

GI number: 13508028

Start: 347169

End: 347732

Strand: Direct

Name: hsdS1B

Synonym: MPN289

Alternate gene names: NA

Gene position: 347169-347732 (Clockwise)

Preceding gene: 13508027

Following gene: 13508029

Centisome position: 42.52

GC content: 36.17

Gene sequence:

>564_bases
GTGCAAATCAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAACTATATATCAAAAA
CAATCCAGGCGAATTCCCTGTTTATTCATCGGCTACAGTTAATAATGGTGAAATTGGCAGAATAAAAGATTGTGATCTTA
AGGGTGAGTATGTGACTTGAACAACTGATGGCGCTCAAGCTGGTAGTGTTTTTTATCGTAACGGTCAATTTAACGCAACC
AATGTTTGTGGAATTTTAAAGGTAAATAATGATGAAATTTACCCTAAATTTTTAGCCTATGCGCTTAGATTGAAAGCACC
TAAATTTGTAAATTATGCTTGCCCTATTCCTAAATTAATGCAAGGAACTTTAGCAGAAATTGAGCTAGATTTCACAAGCA
AGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCC
GAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGTCTTCTATAGCGATTATTTACTTAACCCAAAAAATTGAAA
ATAG

Upstream 100 bases:

>100_bases
GTGAAATTTAAACGAATTTGTGAGCTAACTAATCACAATAATTTCAGGTTAAAACAAAACCTTAAAAATGTTTAATGAAA
GAAATTAAATTGTTACTGTT

Downstream 100 bases:

>100_bases
GAAAATAAATATTACAAGCTGGGAGAAATAGCTCAAAAAGTTTTAGTAGGTGGTGAAAAACCTGCTGATTTTAGTAAGAA
AAAGAACGAAGTTTATAAAT

Product: type I restriction restriction enzyme EcoKI

Products: NA

Alternate protein names: S.MpnORFEBP; Type I restriction enzyme specificity protein MPN_289; S protein [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MQIKTYKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGRIKDCDLKGEYVTWTTDGAQAGSVFYRNGQFNAT
NVCGILKVNNDEIYPKFLAYALRLKAPKFVNYACPIPKLMQGTLAEIELDFTSKKIQEKIATILDTFTELSAELSAELSA
ELSAELRERKKQYVFYSDYLLNPKNWK

Sequences:

>Translated_187_residues
MQIKTYKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGRIKDCDLKGEYVT*TTDGAQAGSVFYRNGQFNAT
NVCGILKVNNDEIYPKFLAYALRLKAPKFVNYACPIPKLMQGTLAEIELDFTSKKIQEKIATILDTFTELSAELSAELSA
ELSAELRERKKQYVFYSDYLLNPKN*K
>Mature_187_residues
MQIKTYKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGRIKDCDLKGEYVT*TTDGAQAGSVFYRNGQFNAT
NVCGILKVNNDEIYPKFLAYALRLKAPKFVNYACPIPKLMQGTLAEIELDFTSKKIQEKIATILDTFTELSAELSAELSA
ELSAELRERKKQYVFYSDYLLNPKN*K

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity [H]

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 20880; Mature: 20880

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKTYKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGRIKDCDLKGEYVTT
CCCEEEEHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEE
TDGAQAGSVFYRNGQFNATNVCGILKVNNDEIYPKFLAYALRLKAPKFVNYACPIPKLMQ
CCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHC
GTLAEIELDFTSKKIQEKIATILDTFTELSAELSAELSAELSAELRERKKQYVFYSDYLL
CCHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEE
NPKNK
CCCCC
>Mature Secondary Structure
MQIKTYKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGRIKDCDLKGEYVTT
CCCEEEEHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEE
TDGAQAGSVFYRNGQFNATNVCGILKVNNDEIYPKFLAYALRLKAPKFVNYACPIPKLMQ
CCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHC
GTLAEIELDFTSKKIQEKIATILDTFTELSAELSAELSAELSAELRERKKQYVFYSDYLL
CCHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEE
NPKNK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]