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The map label for this gene is prrB

Identifier: 13508024

GI number: 13508024

Start: 340613

End: 341533

Strand: Direct

Name: prrB

Synonym: MPN285

Alternate gene names: NA

Gene position: 340613-341533 (Clockwise)

Preceding gene: 13508023

Following gene: 13508025

Centisome position: 41.72

GC content: 38.44

Gene sequence:

>921_bases
ATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCT
AAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCG
AGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGT
GCCGAGCTAAGTGCCGAGCTAAGAGAGCGCAGGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATAT
CCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAATAAGGGATATTTGTGAAATAAATCGTGGTCGAGAAA
TAAATGAAAAATACCTTCGAGAAAATCCAGGTGAATTTCCAGTTTATTCCTCAGCAACAACAAATGGTGGTTTAATTGGA
AAAATTAATGATTACGATTTTCATGGTGAATATGTTACTTGAACAACGGGCGGCGCACATGCCGGAAATGTCTTTTACCG
TAATGAAAAGTTTAGTTGTTCACAAAATTGCGGATTGTTAGAAGTAAAGAACAAAAATAAATTTAGTTCCAAATTCTTAT
GTTTTGCACTAAAACTACAATCTAAAAAATTTGTGAATTATGCCTCTGCAATTCCGGTTCTAACAATAAAGAGAATTGCA
GAAATCGAACTCTCCTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTTTGTTTGCATTTGAAAAACTTTGCAA
CGATTTAACCGAGGGGATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATT
GAGTTCAAAATAAGAAATTAGAAAGTCTCAAATCTCTTTAA

Upstream 100 bases:

>100_bases
AGAAATTAGTTCAAAATATCTTGCTCATATTTTGAAACTAGAAGCACCGAAATTCACAAACCGTGTTTTTAAAAATAGAC
CTAAGTTAACACATAAAACA

Downstream 100 bases:

>100_bases
TAATAAAAGTTAGTTCAGCAAACCACAAACTGATCCCACTTCTCCCCCACGCTCACGCGCTTCTCCACCGGGTTCAACCT
CGCGGGGTCGGTTCTCGACC

Product: type I restriction enzyme ecokI specificity protein

Products: NA

Alternate protein names: S.MpnORFGP; Type I restriction enzyme specificity protein MPN_285; S protein [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MAEIPIDFPPLKIQEKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELS
AELSAELRERRKQYAFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIG
KINDYDFHGEYVTWTTGGAHAGNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIA
EIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFNWVQNKKLESLKSL

Sequences:

>Translated_306_residues
MAEIPIDFPPLKIQEKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELS
AELSAELRERRKQYAFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIG
KINDYDFHGEYVT*TTGGAHAGNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIA
EIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFN*VQNKKLESLKSL
>Mature_305_residues
AEIPIDFPPLKIQEKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSA
ELSAELRERRKQYAFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGK
INDYDFHGEYVT*TTGGAHAGNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAE
IELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFN*VQNKKLESLKSL

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity [H]

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 34285; Mature: 34154

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIPIDFPPLKIQEKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AELSAELSAELSAELSAELSAELSAELRERRKQYAFYRDYLLNQENIRKIYGANIPFETF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCE
QIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVTTTGGAHA
EHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCCCCC
GNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAE
CCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHH
IELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFNVQNKKLES
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
LKSL
HHCC
>Mature Secondary Structure 
AEIPIDFPPLKIQEKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AELSAELSAELSAELSAELSAELSAELRERRKQYAFYRDYLLNQENIRKIYGANIPFETF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCE
QIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVTTTGGAHA
EHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCCCCC
GNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAE
CCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHH
IELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFNVQNKKLES
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
LKSL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]