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The map label for this gene is topA [H]

Identifier: 13508000

GI number: 13508000

Start: 312256

End: 314391

Strand: Direct

Name: topA [H]

Synonym: MPN261

Alternate gene names: 13508000

Gene position: 312256-314391 (Clockwise)

Preceding gene: 13507999

Following gene: 13508001

Centisome position: 38.25

GC content: 40.12

Gene sequence:

>2136_bases
ATGAGTAAAAATCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACGTTGCAAAAATATTTACCGAATGACTTTGAAAT
AGTCTCTACCATTGGTCATATTCGGGAAATGGTGCACAAAAACTTTGGTTTTAACGAAGCCGATTACTCACCAGTTTGGG
AAGACTGAACTAAATCAAAAAAGAAGTTTTCTAGCCTTAGCTTTAAAGGTAATCTTAAGGGCAAAAAACTTTTAAGTAAG
TACGACATCATCAAAAGCATTAAAGAGAAGGCTAGTAAAGCCACTAACATTTACCTAGCAACCGACCCAGACCGTGAGGG
AGAGGCAATTTCCTGACATGTTTACGATGTTTTGGATGAAAAGGACAAGTCGAAGTGCCAACGCATTACCTTTAACGAAA
TTACCAAGAACGCCGTTTTAGATGCACTGAAAAACCCACGGGAAATTGATCAGAGTTGGGTGCAGAGTCAGTTTGCGCGC
CAAATTCTTGACCGGATGATTGGTTTTCGCCTTTCAAGGCTGTTAAACAATTACCTCTCAGCTAAGTCAGCGGGACGGGT
ACAATCAGTAGCACTGCGTTTTTTAGAAGAACGTGAACAGGAAATTCGTAGCTTTGTACCACGGTTTTGGTGAACGCTCG
ATGTGTTGTTAAATCCCAAGGCTGAAGGTGTTAGGGAGGCGTGTGCTAATCGTTCAATTCCAATTGTCTTGCGTGAAATT
AATCCTGCTTTACGTGCTGGCTTGAAATTTGAAGAGGAGAAGAGCGTATCGGGAATTGACTTTTTAGATGAAGCATCAGC
TAAAAAGTTTGGTGAACAGCTCAAGGGTGTCTTTGAGGTGTACAACATTGATGAAACCAAACACTACAGCAGTTCGCCCA
ATTCCGCTTACACCACCGCTTCATTGCAAAAGGATGCCATCAACAAACTAGGTTGGTCTTCCAAAAAGGTAACCTTAATT
GCCCAACACCTGTATGAAGGGGTGAGTATTAATGGAGAACAAACCGCTTTAATTAGCTATCCCCGTACCGACTCAACTCG
TTTATCAGCGCAATTTCAACAAAGTTGTAAAGAATACATCTTAAACCATTACGGAGAAAAGTACCTCTCTAATCGGATTG
TAAGCGCTAAGGGAAAAAAAGGTGAAAAAATTATCCAAGACGCCCATGAAGCAATTCACCCAACTGACATTAACATTACC
CCGGAAATGGTCAAGAATGCGATTAAGAAGGATGAGTTCTTACTGTACCGCTTAATCTGGATCCGTACGGTCGCTAGTTT
AATGGCGGATTGTAAAAAATCACATACCCACATCCGGTTTATTAACGATGGTAATAAGTTTTACGCTTCTTCCAAGTCAT
TAGTATTTGATGGTTATCGCAAAATTTACGAACACTTTGAAAATAAGGAAAGTAATGATCTCTACATTGATTTGGATAAG
ATTAGGGTTGGTGATCGTTTCATGGCTAAAGACATTAAGATCACAGCCCGCCAAACCCATCCAGCAGCCCGTTATACCCA
AGCTAGTTTAATTGAAGCGTTAGAAAAGTCCAATATTGGTCGACCTTCCACCTATAACACGATGGCTAGTGTTAATTTAG
ACCGGGGTTATGCTAGTTTAAATAAACATGCTTTTCACGTAACACAACTAGGGGAGCAGGTTAATGAAGAATTGTCCAAA
CACTTTGGTAAGATCATTAACAAAGAGTTCACTAAAAACATGGAGAAGTCTTTGGATGAAATTGCTGAAAATAAAAAGAA
CTACCAGGAGTTTCTCCGTGATTTTTGGTCAAACTTTAAGGAAGAGGTAAAGCTAGCGGAGGGTTCGATTCAAAGGGTGA
AAAAGGAAAAGGAATTTGTTGGTCGTGATTGTCCAAGCTGTGCTAGTCCCTTGTTGTACCGCTATACTAAACGGGGTAAT
GAAAAGTTTGTTGGGTGCAGTAACTTTCCCAACTGTAAATACAACGAGTTTAGCCAAAATAAACCGAATTTAACTTTGGA
AAAGCTCGAAGAACTGTGTCCGGAGTGTAACAGCCAACTAGTGAAAAGACGCACTAAGTTTAATCCCAATAAAACCTTTG
TTGGTTGTAGTAATTTCCCTAGGTGCCGCTACATAAAGAAGGATAATGCTAGCTAG

Upstream 100 bases:

>100_bases
ATTTGGATATCTCAATGTAGGGCCGAAAAACGTTGGTAAGCATTTCGATAAGGGTTTGCGATAGGTTAACCTTTATTTAT
AGTTATAGTCAAGCCAACAA

Downstream 100 bases:

>100_bases
TTTAACGAGCAGAAGTGCCACTTCCTTCTCAATAATTTGAGCGTTGGTAAGTGCCATTTTGATCTTAAGTATCCTCATTT
GGCTTATTATCACCATTTTC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 711; Mature: 710

Protein sequence:

>711_residues
MSKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWEDWTKSKKKFSSLSFKGNLKGKKLLSK
YDIIKSIKEKASKATNIYLATDPDREGEAISWHVYDVLDEKDKSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFAR
QILDRMIGFRLSRLLNNYLSAKSAGRVQSVALRFLEEREQEIRSFVPRFWWTLDVLLNPKAEGVREACANRSIPIVLREI
NPALRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQKDAINKLGWSSKKVTLI
AQHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNHYGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDINIT
PEMVKNAIKKDEFLLYRLIWIRTVASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDK
IRVGDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKHAFHVTQLGEQVNEELSK
HFGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEVKLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGN
EKFVGCSNFPNCKYNEFSQNKPNLTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS

Sequences:

>Translated_711_residues
MSKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWED*TKSKKKFSSLSFKGNLKGKKLLSK
YDIIKSIKEKASKATNIYLATDPDREGEAIS*HVYDVLDEKDKSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFAR
QILDRMIGFRLSRLLNNYLSAKSAGRVQSVALRFLEEREQEIRSFVPRFW*TLDVLLNPKAEGVREACANRSIPIVLREI
NPALRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQKDAINKLGWSSKKVTLI
AQHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNHYGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDINIT
PEMVKNAIKKDEFLLYRLIWIRTVASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDK
IRVGDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKHAFHVTQLGEQVNEELSK
HFGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEVKLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGN
EKFVGCSNFPNCKYNEFSQNKPNLTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS
>Mature_710_residues
SKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWED*TKSKKKFSSLSFKGNLKGKKLLSKY
DIIKSIKEKASKATNIYLATDPDREGEAIS*HVYDVLDEKDKSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFARQ
ILDRMIGFRLSRLLNNYLSAKSAGRVQSVALRFLEEREQEIRSFVPRFW*TLDVLLNPKAEGVREACANRSIPIVLREIN
PALRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQKDAINKLGWSSKKVTLIA
QHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNHYGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDINITP
EMVKNAIKKDEFLLYRLIWIRTVASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDKI
RVGDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKHAFHVTQLGEQVNEELSKH
FGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEVKLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGNE
KFVGCSNFPNCKYNEFSQNKPNLTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=601, Percent_Identity=23.7936772046589, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI10835218, Length=652, Percent_Identity=23.159509202454, Blast_Score=100, Evalue=6e-21,
Organism=Escherichia coli, GI1787529, Length=736, Percent_Identity=30.9782608695652, Blast_Score=325, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI32563869, Length=728, Percent_Identity=21.978021978022, Blast_Score=109, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17555378, Length=537, Percent_Identity=24.5810055865922, Blast_Score=102, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81353; Mature: 81222

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWEDTKSKK
CCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
KFSSLSFKGNLKGKKLLSKYDIIKSIKEKASKATNIYLATDPDREGEAISHVYDVLDEKD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCCC
KSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFARQILDRMIGFRLSRLLNNYLSAK
HHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SAGRVQSVALRFLEEREQEIRSFVPRFWTLDVLLNPKAEGVREACANRSIPIVLREINPA
CCCHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCHHHHHHHHCCCCCCEEEECCCHH
LRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQ
HHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEHHHHH
KDAINKLGWSSKKVTLIAQHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNH
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
YGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDINITPEMVKNAIKKDEFLLYRLIWIRT
HHHHHHHCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDKIRV
HHHHHHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHEEC
GDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKH
CCHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEECCCCHHHHHHH
AFHVTQLGEQVNEELSKHFGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGNEKFVGCSNFPNCKYNEFSQNKPN
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCC
LTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEECCCCCCC
>Mature Secondary Structure 
SKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWEDTKSKK
CCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
KFSSLSFKGNLKGKKLLSKYDIIKSIKEKASKATNIYLATDPDREGEAISHVYDVLDEKD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCCC
KSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFARQILDRMIGFRLSRLLNNYLSAK
HHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SAGRVQSVALRFLEEREQEIRSFVPRFWTLDVLLNPKAEGVREACANRSIPIVLREINPA
CCCHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCHHHHHHHHCCCCCCEEEECCCHH
LRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQ
HHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEHHHHH
KDAINKLGWSSKKVTLIAQHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNH
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
YGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDINITPEMVKNAIKKDEFLLYRLIWIRT
HHHHHHHCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDKIRV
HHHHHHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHEEC
GDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKH
CCHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEECCCCHHHHHHH
AFHVTQLGEQVNEELSKHFGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGNEKFVGCSNFPNCKYNEFSQNKPN
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCC
LTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]