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The map label for this gene is vacB [H]

Identifier: 13507982

GI number: 13507982

Start: 294381

End: 296561

Strand: Direct

Name: vacB [H]

Synonym: MPN243

Alternate gene names: 13507982

Gene position: 294381-296561 (Clockwise)

Preceding gene: 13507981

Following gene: 13507983

Centisome position: 36.06

GC content: 42.64

Gene sequence:

>2181_bases
ATGAAAGTATTAACTGATTTACAAAAGCGAATCTTCGCGATTGTGAAAAAAGAGAACGGCAAACCTATTCCTCCGGGAAT
AGTTGTCAGGATGATGGAGAATCAAGCTGGTTTTCCGGGAAAGCAACAGGTATACAGAGCAATTGATGACTTGTTAGAAT
GACACATTTTTCGCAAGTCGGGTGGTGCTACAAACCAGCTGTTAATTAACTATGAACTAGCTGATCCGGTATTGGATCAA
AAGTTTCAGGGCATTCTCAATTTAGGTAACAAGAACACTGGTTTTGTCCGTCCCTTAGATGATGACAAAACGGTGTACTA
TATCCATTTTTCCAACCTAGCGGGAGCACTGGATGGTGATCTAGTTGAATTCTGTCCGCTCGACAAACCACAGGTAGGTG
ATAAGTTTGATGCTGCAGTGCTCAAAATTGTGAAGCGCTCCCGGGTGCTGTACGCTGGTAACTTTTTGATTGAGTACTCC
GATTTTGGTCAGGAGTTTCGCATTGTTGCTGACAATCCTCGTTTTTACCTCACCCCGATTGTCAATAAGGCCTCAGTTCC
AGCTGAACTGGAGAGCAATACTAAGGTCGCTTTTCAAATTGATGAGTATGATCCTGCAAATAACCTGTGTAAAGTCTCCA
TTCAGCAAATCTTGGGGAATAACGATGAACCGTTAATTAACCTCAAGGCGATTATGTTAGACCACTCGATTGTCTTTGAA
GATAATGATGTGGTCGAACAACAAGCAGCGAAGTTACAGTTTGATGAAAAAGAGCAGAGTAAGCCCTACCGCAAGGACTT
AACCGAACTTGCCTTTGTCACGATTGACCCCGCCACTTCCAAGGACTTGGATGATGCGATTTACGTCAAGCGCACCGACA
AAGGTTTTGTTTTGTATGTTGCCATTGCTGATGTGGCTTACTATGTGCAGCGCAACAGTGAACTTGACATTGAAGCCCGT
CATAAAACAAGCTCAATTTACCTCCCGGGATACTATGTGGTACCGATGTTACCAGAACGTTTATCAAACGAGCTGTGTTC
TTTAAACCCAAATGAAAAGCGGTATGTGGTGGTGTGTGAGTTGAACTTTGATCATGAAGCACGCTTAAACTTTAGTGAAG
TGTATCCCGCTACCATTGTCTCCCAACGTCGCTTTGCTTACAGTGAAGTTAATGATTGACTGGAAGACAGTGATGCTCTA
AAGGATGAAAGTGCAACAGTACTAGAGAGCTTAAAGGCTGGTTTTACCTTGAGCGAATTGATTGCTGAACAGCGCAAAAA
GAAGGGGACTATTGACCTATCTCACAGTGAAACAGAAGTAGTCGTTGATCAAAACTACTACCCGATTGAGATCCGTTTTC
TCACCCACGGCAAGGCGGAAACAATGATCGAAAACCTAATGGTGGTTGCCAATGAAGCGGTGGCTTGAACACTTACAAAC
CACAAAGTTCACTTACCTTACCGGGTGCATCCCCGACCAAGCAAAAAGAAGTTACAAATGTTGTTGGAAAACATTGTCGA
GCTCAAAATTACCCAACCAAACTTTGTACTCGATACGGTCACTTCCACCCAAATTGCGGCTTGGTTAAAGGAAAATAAGG
ACAACCCGAGCTATGACATCTTTGTCATCTTGTTATTAAGAACGCTAGGTAAAGCCTTTTACATTGTTAATCCACTGATA
CACTTTAGCATTGGTTCACACCACTATACCCATTTCACTTCGCCCATCCGTCGCTATGCTGATTTAACGGTACACCGTTT
GTTGTGGATGAATCTGTTTACTCCAGAGCGTTTTACTGACACTGAGCGTGAACAGCTCAACGCCGAGCTAGAACAAATTT
GTGAAACCATTAACGATACTGAGATTAAGATTAATGGTTGTGAACGGACTGCCAACGATTACTTAACTACGCTGTATTTG
TCTAAACAAGTGGGACAAACTTTCCACGGTTTTATTTCCGCTATTACTAGTTTTGGCATCTTTATGCGAATGGATGAAAA
CAACTTTGATGGACTTATTAAGATTACCTCCATTCCCGAAGACTTCTTTGTCTTTGAAAAGGATCGGATGGTGTTGCGCG
GCAAACGCACCAACAAGGTTTTTAGAATTGGTGATAGGTTAACCGCGAAACTGACTGAAATTGATACAGTGCAGAAACGC
GCCATTCTAACCCTGGTTTAA

Upstream 100 bases:

>100_bases
CGCGGAATTGTCAAAATTCTCCAAATTACGATGTTTATTTTAGTAGTGCTTTTTCTTATTTTAGGGTTAGTTTTTCATTT
TGCGCTTTAAAATCTAACAG

Downstream 100 bases:

>100_bases
TGATGACAGTGGAAGTCTTTTCGGTTATTATCCTTGTCCTTTTATTCTTAATCTTAGCGCTTACCTTGTTGTTTGTTTTA
CTCAACAAGCGTACTAGAAG

Product: 3'-5' exoribonuclease, RNase R

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 726; Mature: 726

Protein sequence:

>726_residues
MKVLTDLQKRIFAIVKKENGKPIPPGIVVRMMENQAGFPGKQQVYRAIDDLLEWHIFRKSGGATNQLLINYELADPVLDQ
KFQGILNLGNKNTGFVRPLDDDKTVYYIHFSNLAGALDGDLVEFCPLDKPQVGDKFDAAVLKIVKRSRVLYAGNFLIEYS
DFGQEFRIVADNPRFYLTPIVNKASVPAELESNTKVAFQIDEYDPANNLCKVSIQQILGNNDEPLINLKAIMLDHSIVFE
DNDVVEQQAAKLQFDEKEQSKPYRKDLTELAFVTIDPATSKDLDDAIYVKRTDKGFVLYVAIADVAYYVQRNSELDIEAR
HKTSSIYLPGYYVVPMLPERLSNELCSLNPNEKRYVVVCELNFDHEARLNFSEVYPATIVSQRRFAYSEVNDWLEDSDAL
KDESATVLESLKAGFTLSELIAEQRKKKGTIDLSHSETEVVVDQNYYPIEIRFLTHGKAETMIENLMVVANEAVAWTLTN
HKVHLPYRVHPRPSKKKLQMLLENIVELKITQPNFVLDTVTSTQIAAWLKENKDNPSYDIFVILLLRTLGKAFYIVNPLI
HFSIGSHHYTHFTSPIRRYADLTVHRLLWMNLFTPERFTDTEREQLNAELEQICETINDTEIKINGCERTANDYLTTLYL
SKQVGQTFHGFISAITSFGIFMRMDENNFDGLIKITSIPEDFFVFEKDRMVLRGKRTNKVFRIGDRLTAKLTEIDTVQKR
AILTLV

Sequences:

>Translated_726_residues
MKVLTDLQKRIFAIVKKENGKPIPPGIVVRMMENQAGFPGKQQVYRAIDDLLE*HIFRKSGGATNQLLINYELADPVLDQ
KFQGILNLGNKNTGFVRPLDDDKTVYYIHFSNLAGALDGDLVEFCPLDKPQVGDKFDAAVLKIVKRSRVLYAGNFLIEYS
DFGQEFRIVADNPRFYLTPIVNKASVPAELESNTKVAFQIDEYDPANNLCKVSIQQILGNNDEPLINLKAIMLDHSIVFE
DNDVVEQQAAKLQFDEKEQSKPYRKDLTELAFVTIDPATSKDLDDAIYVKRTDKGFVLYVAIADVAYYVQRNSELDIEAR
HKTSSIYLPGYYVVPMLPERLSNELCSLNPNEKRYVVVCELNFDHEARLNFSEVYPATIVSQRRFAYSEVND*LEDSDAL
KDESATVLESLKAGFTLSELIAEQRKKKGTIDLSHSETEVVVDQNYYPIEIRFLTHGKAETMIENLMVVANEAVA*TLTN
HKVHLPYRVHPRPSKKKLQMLLENIVELKITQPNFVLDTVTSTQIAAWLKENKDNPSYDIFVILLLRTLGKAFYIVNPLI
HFSIGSHHYTHFTSPIRRYADLTVHRLLWMNLFTPERFTDTEREQLNAELEQICETINDTEIKINGCERTANDYLTTLYL
SKQVGQTFHGFISAITSFGIFMRMDENNFDGLIKITSIPEDFFVFEKDRMVLRGKRTNKVFRIGDRLTAKLTEIDTVQKR
AILTLV
>Mature_726_residues
MKVLTDLQKRIFAIVKKENGKPIPPGIVVRMMENQAGFPGKQQVYRAIDDLLE*HIFRKSGGATNQLLINYELADPVLDQ
KFQGILNLGNKNTGFVRPLDDDKTVYYIHFSNLAGALDGDLVEFCPLDKPQVGDKFDAAVLKIVKRSRVLYAGNFLIEYS
DFGQEFRIVADNPRFYLTPIVNKASVPAELESNTKVAFQIDEYDPANNLCKVSIQQILGNNDEPLINLKAIMLDHSIVFE
DNDVVEQQAAKLQFDEKEQSKPYRKDLTELAFVTIDPATSKDLDDAIYVKRTDKGFVLYVAIADVAYYVQRNSELDIEAR
HKTSSIYLPGYYVVPMLPERLSNELCSLNPNEKRYVVVCELNFDHEARLNFSEVYPATIVSQRRFAYSEVND*LEDSDAL
KDESATVLESLKAGFTLSELIAEQRKKKGTIDLSHSETEVVVDQNYYPIEIRFLTHGKAETMIENLMVVANEAVA*TLTN
HKVHLPYRVHPRPSKKKLQMLLENIVELKITQPNFVLDTVTSTQIAAWLKENKDNPSYDIFVILLLRTLGKAFYIVNPLI
HFSIGSHHYTHFTSPIRRYADLTVHRLLWMNLFTPERFTDTEREQLNAELEQICETINDTEIKINGCERTANDYLTTLYL
SKQVGQTFHGFISAITSFGIFMRMDENNFDGLIKITSIPEDFFVFEKDRMVLRGKRTNKVFRIGDRLTAKLTEIDTVQKR
AILTLV

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014625, Length=564, Percent_Identity=25.177304964539, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI190014623, Length=564, Percent_Identity=25.177304964539, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI134288890, Length=440, Percent_Identity=27.0454545454545, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI219521928, Length=556, Percent_Identity=26.6187050359712, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI19115966, Length=556, Percent_Identity=26.6187050359712, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI156105695, Length=377, Percent_Identity=23.8726790450928, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI156105693, Length=377, Percent_Identity=24.1379310344828, Blast_Score=77, Evalue=8e-14,
Organism=Escherichia coli, GI87082383, Length=651, Percent_Identity=27.8033794162826, Blast_Score=266, Evalue=4e-72,
Organism=Escherichia coli, GI1787542, Length=479, Percent_Identity=27.7661795407098, Blast_Score=130, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17553506, Length=416, Percent_Identity=28.6057692307692, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI212645896, Length=540, Percent_Identity=25.9259259259259, Blast_Score=137, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324552, Length=434, Percent_Identity=25.5760368663594, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24649634, Length=608, Percent_Identity=24.6710526315789, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI19922976, Length=370, Percent_Identity=28.3783783783784, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24654597, Length=370, Percent_Identity=28.3783783783784, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24654592, Length=420, Percent_Identity=27.8571428571429, Blast_Score=111, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805 [H]

Pfam domain/function: PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 82608; Mature: 82608

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLTDLQKRIFAIVKKENGKPIPPGIVVRMMENQAGFPGKQQVYRAIDDLLEHIFRKSG
CCHHHHHHHHHHEEEECCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
GATNQLLINYELADPVLDQKFQGILNLGNKNTGFVRPLDDDKTVYYIHFSNLAGALDGDL
CCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCH
VEFCPLDKPQVGDKFDAAVLKIVKRSRVLYAGNFLIEYSDFGQEFRIVADNPRFYLTPIV
HHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCEEEEECCCCCEEEEEECCCCEEEEECC
NKASVPAELESNTKVAFQIDEYDPANNLCKVSIQQILGNNDEPLINLKAIMLDHSIVFED
CCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECEEEECC
NDVVEQQAAKLQFDEKEQSKPYRKDLTELAFVTIDPATSKDLDDAIYVKRTDKGFVLYVA
CHHHHHHHHHCCCCCHHCCCCHHHHHHHEEEEEECCCCCCCCCCEEEEEECCCCEEEEEE
IADVAYYVQRNSELDIEARHKTSSIYLPGYYVVPMLPERLSNELCSLNPNEKRYVVVCEL
HHHHHHHHHCCCCCCEEECCCCCEEEECCEEEECCCCHHHCCHHHCCCCCCCEEEEEEEE
NFDHEARLNFSEVYPATIVSQRRFAYSEVNDLEDSDALKDESATVLESLKAGFTLSELIA
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHCCCCHHHHHH
EQRKKKGTIDLSHSETEVVVDQNYYPIEIRFLTHGKAETMIENLMVVANEAVATLTNHKV
HHHHCCCCEEECCCCCEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHEECCCEE
HLPYRVHPRPSKKKLQMLLENIVELKITQPNFVLDTVTSTQIAAWLKENKDNPSYDIFVI
ECCEEECCCCCHHHHHHHHHHHHEEEECCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHH
LLLRTLGKAFYIVNPLIHFSIGSHHYTHFTSPIRRYADLTVHRLLWMNLFTPERFTDTER
HHHHHHCCCEEEECHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EQLNAELEQICETINDTEIKINGCERTANDYLTTLYLSKQVGQTFHGFISAITSFGIFMR
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
MDENNFDGLIKITSIPEDFFVFEKDRMVLRGKRTNKVFRIGDRLTAKLTEIDTVQKRAIL
ECCCCCCCEEEEECCCCHHEEEECCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHHEEE
TLV
EEC
>Mature Secondary Structure
MKVLTDLQKRIFAIVKKENGKPIPPGIVVRMMENQAGFPGKQQVYRAIDDLLEHIFRKSG
CCHHHHHHHHHHEEEECCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
GATNQLLINYELADPVLDQKFQGILNLGNKNTGFVRPLDDDKTVYYIHFSNLAGALDGDL
CCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCH
VEFCPLDKPQVGDKFDAAVLKIVKRSRVLYAGNFLIEYSDFGQEFRIVADNPRFYLTPIV
HHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCEEEEECCCCCEEEEEECCCCEEEEECC
NKASVPAELESNTKVAFQIDEYDPANNLCKVSIQQILGNNDEPLINLKAIMLDHSIVFED
CCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECEEEECC
NDVVEQQAAKLQFDEKEQSKPYRKDLTELAFVTIDPATSKDLDDAIYVKRTDKGFVLYVA
CHHHHHHHHHCCCCCHHCCCCHHHHHHHEEEEEECCCCCCCCCCEEEEEECCCCEEEEEE
IADVAYYVQRNSELDIEARHKTSSIYLPGYYVVPMLPERLSNELCSLNPNEKRYVVVCEL
HHHHHHHHHCCCCCCEEECCCCCEEEECCEEEECCCCHHHCCHHHCCCCCCCEEEEEEEE
NFDHEARLNFSEVYPATIVSQRRFAYSEVNDLEDSDALKDESATVLESLKAGFTLSELIA
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHCCCCHHHHHH
EQRKKKGTIDLSHSETEVVVDQNYYPIEIRFLTHGKAETMIENLMVVANEAVATLTNHKV
HHHHCCCCEEECCCCCEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHEECCCEE
HLPYRVHPRPSKKKLQMLLENIVELKITQPNFVLDTVTSTQIAAWLKENKDNPSYDIFVI
ECCEEECCCCCHHHHHHHHHHHHEEEECCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHH
LLLRTLGKAFYIVNPLIHFSIGSHHYTHFTSPIRRYADLTVHRLLWMNLFTPERFTDTER
HHHHHHCCCEEEECHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EQLNAELEQICETINDTEIKINGCERTANDYLTTLYLSKQVGQTFHGFISAITSFGIFMR
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
MDENNFDGLIKITSIPEDFFVFEKDRMVLRGKRTNKVFRIGDRLTAKLTEIDTVQKRAIL
ECCCCCCCEEEEECCCCHHEEEECCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHHEEE
TLV
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]