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The map label for this gene is dnaB [H]

Identifier: 13507971

GI number: 13507971

Start: 281673

End: 283094

Strand: Direct

Name: dnaB [H]

Synonym: MPN232

Alternate gene names: 13507971

Gene position: 281673-283094 (Clockwise)

Preceding gene: 13507970

Following gene: 13507972

Centisome position: 34.5

GC content: 39.52

Gene sequence:

>1422_bases
ATGGTGTCAAATAAGCTTGAACAGACCTTTAAGTTTGCCAACGACAAGAACATTGAACGTGCTGAGCGTGTTTTAATGCA
AGCTTTAGTACAAGATGCCGTTGGTGTGGACTTAATCTTAACCAAGCTTGAACCGAAGGACTTCTTTGGTTTTCCGTTTA
ACTTTATCTTTCAAACAGCACAGGAAAACTATTCTGAGGGCAATAAGTTGTTTGGTTCGGCGCTTTTAGAGGCCGTTAAG
TTTAAGCTGGATACCGATTCCAAAACACAAAGGGAAATTGAAGGTTTATTTGAGGACGTTTTACTCAAAGGTCTTCCCTT
TTTGGAAAGGGACATCAAGGTCTTTGTTGATGTTGTCAAAAAAGCTTCGATTTTTCGCCAACTTAAACAGTTCGCCAAAA
AGGTTGAAAAGGAAGAGTTTAAGGTCAAAGATGACCGTTTTGAGGGCTATTTACAAGCAATTCAAAACGACTTTACCAAG
ATTATTCACAGCGCCTTTGTTTCTATTCCCGGTTTGAGTTATGACGAAATTGTTCGTGAAGAGGAAGAGTTAATTCCCAA
AGTGTACCGTGGTGAATTAACTGTAAAAGGGCTTAAAAGTGGTTTTTACCCTTTAGATCAATTAACCTCTGGTTGAAAAG
AGGGTGAGTTAATTGTGGTAGCAGCGCGTCCAGGTCGCGGGAAAACAGCGCTTCTAATTAACTTTTTGCAGGGCGCGGTT
AATGACAGCGAAAAGTTTGATAAAAACAAGGATGTGTTGCTGTTTTTTAGCTTAGAAATGCGTAACCGTGAAATTTATCA
GCGCCATTTAATGCTAGAAAGCCAGGTAAATTACACACTCACGAGTCGTCAAAGGCTCGCTAATGTGTATGATGATTTAA
TTAAAGCATCAGAAGTATTGCGCGATCTTCCTATTAAAATTTTTGATTACAGCACGATTACGTTGGACGAAATTCGTGCT
CAAATCACGGAAGTTTCAAAAACCAATAACGTTAAGTTGGTCGTAATTGACTATCTCCAACTAGTTAATGCATTTAAAAA
CAGCTATAACATTTCGCGTCAACAAGAGGTCACCATGATTTCCAAATCACTTAAAAGCTTTGCCAAGGAGTTTAACGTAC
CAATTATTGCCGCTGCACAGCTTTCTCGAAGAATAGAAGAACGCAAAGATTCGCGTCCCATTTTGTCGGATTTACGTGAA
TCGGGTTCGATTGAACAGGATGCTGACATGGTTTTATTCATCCACCGCGTCAAGGATGAGGACAGTGAAAATCAAGAAGC
AATTGGTCACAACAATATTTTTCAAGTGGAGTTAATCTTGGAAAAAAACCGTAGTGGTCCGACTGGTAAAGTACAGTTTG
ACTTTCAAAGTGACGTTTCTACCTTTCGGGTTCGGGAATACAATCCTGATGGTTACAGTTAG

Upstream 100 bases:

>100_bases
TTTAAAGACAACGTGCGCTTAGGGTTTGGGGATCACGAGATTACCTTACACATCTTTGAAGATACAACCGCAGTTTTAAA
AGTTAAGGTAACCCCGGATA

Downstream 100 bases:

>100_bases
TTTATGAAGTACAAAACAGTTAAATCGATCCCGCTTTTTTTGTTAGGCTCGATTGTGTTCACTGCTTGTTCAACACCACA
ATCAACCTTTCATTTACCTG

Product: replicative DNA helicase DnaB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVK
FKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTK
IIHSAFVSIPGLSYDEIVREEEELIPKVYRGELTVKGLKSGFYPLDQLTSGWKEGELIVVAARPGRGKTALLINFLQGAV
NDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRA
QITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRE
SGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS

Sequences:

>Translated_473_residues
MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVK
FKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTK
IIHSAFVSIPGLSYDEIVREEEELIPKVYRGELTVKGLKSGFYPLDQLTSG*KEGELIVVAARPGRGKTALLINFLQGAV
NDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRA
QITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRE
SGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS
>Mature_473_residues
MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVK
FKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTK
IIHSAFVSIPGLSYDEIVREEEELIPKVYRGELTVKGLKSGFYPLDQLTSG*KEGELIVVAARPGRGKTALLINFLQGAV
NDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRA
QITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRE
SGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=454, Percent_Identity=31.2775330396476, Blast_Score=166, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694 [H]

Pfam domain/function: PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54287; Mature: 54287

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTA
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCHHHHHHHH
QENYSEGNKLFGSALLEAVKFKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVK
HHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTKIIHSAFVSIPGLSYDEIVRE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EEELIPKVYRGELTVKGLKSGFYPLDQLTSGKEGELIVVAARPGRGKTALLINFLQGAVN
HHHHHHHHHCCCEEEEHHHCCCCCHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
DSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLR
CHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHH
DLPIKIFDYSTITLDEIRAQITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMIS
HCCCEEECCCEEEHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHH
KSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRVKDED
HHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCC
SENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS
CCCCHHCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTA
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCHHHHHHHH
QENYSEGNKLFGSALLEAVKFKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVK
HHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTKIIHSAFVSIPGLSYDEIVRE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EEELIPKVYRGELTVKGLKSGFYPLDQLTSGKEGELIVVAARPGRGKTALLINFLQGAVN
HHHHHHHHHCCCEEEEHHHCCCCCHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
DSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLR
CHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHH
DLPIKIFDYSTITLDEIRAQITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMIS
HCCCEEECCCEEEHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHH
KSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRVKDED
HHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCC
SENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS
CCCCHHCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]