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The map label for this gene is truA [H]

Identifier: 13507935

GI number: 13507935

Start: 235805

End: 236536

Strand: Direct

Name: truA [H]

Synonym: MPN196

Alternate gene names: 13507935

Gene position: 235805-236536 (Clockwise)

Preceding gene: 13507934

Following gene: 13507936

Centisome position: 28.88

GC content: 42.49

Gene sequence:

>732_bases
GTGCCTAGGTACTTAGCGATTGTCGCTTACAATGGTGCTCCCTTTCGTGGTTGAGCCAAACAACCGGGTTACCACACCGT
TCAGGGTCAAATAGAACAAAACCTAGCCTTAATTTACCAAACACCAATTAATATTTATGGTTCGGGTCGGACTGATAAGG
GAGTGCATGCCATTAACCAAACCTTTCACGTTGATCTACCTGATAAGTTACCACTCAAACAGTTAATTGCCAAACTAAAC
CAATTAAACTACCCTGACATTTTTGTAAAGGCCTTAGTTCCAGTAGCACCGCGATTTCACGCGCGGTTTGCAGTAAAAAC
TAAGGTTTATGAATACCTTATTAACACCCGAGCTTTTAACCCAGCACAGTACCACACCGTGTGACAGTACAACCACCCAT
TGGATTTAGCACAACTTGAAGCGGATACCACGCTCTTTTTAGGCAGGAAGGATTTTTGGAGTTTTAGCACCGCTAGCCAA
AAGGACACGCAGCGCACAATTAGTAAAATTATCGTGCAACAAGATCACACTGGTACGGTTAAACTGACCTTTTTTGGCAA
CGGTTTTTTAAGAAACCAGATTCGAATGATGGTAGCTTCTCTAGTGGCCTTAAACAATCGAACCCTGATGCGAGAAACAG
TTCAACAGCTGTTTGATCAACCACAAAAGGGTGGGTGTAAGGTTAAAGCCCCCGCTTGTGGTCTTTATTTAAAAACTGTC
TTATATGAATAA

Upstream 100 bases:

>100_bases
TTAACCGTTAAGGGTGCAGTGTTCCTAGATTTAGGGACACCTGAGTGGTTGCTAACCGGCAAGATTAAAGAGCAGGTAGA
GCGCTCATTATCAGTTAAAA

Downstream 100 bases:

>100_bases
CCAATACAACTGAAACCTCGAAGTTTTACTAAATGGTAAGAGTTTAGCTGATAACTTTACCGAACTCAAACAACTCAGTG
AGCAGGAAAAAGCACTTTAC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MPRYLAIVAYNGAPFRGWAKQPGYHTVQGQIEQNLALIYQTPINIYGSGRTDKGVHAINQTFHVDLPDKLPLKQLIAKLN
QLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNPAQYHTVWQYNHPLDLAQLEADTTLFLGRKDFWSFSTASQ
KDTQRTISKIIVQQDHTGTVKLTFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTV
LYE

Sequences:

>Translated_243_residues
MPRYLAIVAYNGAPFRG*AKQPGYHTVQGQIEQNLALIYQTPINIYGSGRTDKGVHAINQTFHVDLPDKLPLKQLIAKLN
QLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNPAQYHTV*QYNHPLDLAQLEADTTLFLGRKDFWSFSTASQ
KDTQRTISKIIVQQDHTGTVKLTFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTV
LYE
>Mature_242_residues
PRYLAIVAYNGAPFRG*AKQPGYHTVQGQIEQNLALIYQTPINIYGSGRTDKGVHAINQTFHVDLPDKLPLKQLIAKLNQ
LNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNPAQYHTV*QYNHPLDLAQLEADTTLFLGRKDFWSFSTASQK
DTQRTISKIIVQQDHTGTVKLTFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTVL
YE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=29.0441176470588, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788657, Length=239, Percent_Identity=27.1966527196653, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27309; Mature: 27178

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYLAIVAYNGAPFRGAKQPGYHTVQGQIEQNLALIYQTPINIYGSGRTDKGVHAINQT
CCCEEEEEEECCCCCCCCCCCCCEEECCHHCCCEEEEEECCEEEEECCCCCCCHHHHCCE
FHVDLPDKLPLKQLIAKLNQLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNP
EEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AQYHTVQYNHPLDLAQLEADTTLFLGRKDFWSFSTASQKDTQRTISKIIVQQDHTGTVKL
CCEEEEEECCCCCHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
TFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTVLY
EEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHC
E
C
>Mature Secondary Structure 
PRYLAIVAYNGAPFRGAKQPGYHTVQGQIEQNLALIYQTPINIYGSGRTDKGVHAINQT
CCEEEEEEECCCCCCCCCCCCCEEECCHHCCCEEEEEECCEEEEECCCCCCCHHHHCCE
FHVDLPDKLPLKQLIAKLNQLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNP
EEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AQYHTVQYNHPLDLAQLEADTTLFLGRKDFWSFSTASQKDTQRTISKIIVQQDHTGTVKL
CCEEEEEECCCCCHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
TFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTVLY
EEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8604303; 8948633 [H]