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The map label for this gene is grpE [H]

Identifier: 13507859

GI number: 13507859

Start: 155323

End: 155976

Strand: Direct

Name: grpE [H]

Synonym: MPN120

Alternate gene names: 13507859

Gene position: 155323-155976 (Clockwise)

Preceding gene: 13507858

Following gene: 13507860

Centisome position: 19.03

GC content: 39.45

Gene sequence:

>654_bases
ATGAGCGAAAACTCACTCACAATTACCGAAATTCTCAGTTCCATCCGTACGCTTTTAGTCAAACACAATAAGGCTAAAGT
AACCCAAATTGAAAAGGAACTTTTACAAGCAGTTGCTGAACTGGAAAAGAAGTTTAAGCAACAAGTACAAAACTTCAACG
AGTTGCAACAAAAGATTCCTAACTTACAAAAAGTCAACGAGGAATTTCGCTTAAAAGTGGAAAAAATCCAGGAAGAAGCG
CAGAAGAAAATTCAGGAAAAAGTGGCAGAACTGACCATTAAAAGTAAGGAAGAGTTGGAAAATGCAAAGAAGTATGTGAT
CGAAAAGTCGATTGATCAACCCTTAATTATTATTGACCAGTTCGAAATTGCACTTTCCTATGCTCAAAAGGATCCCCAAG
TAAAAAACTATACCACTGGGTTTAACATGGTGTTGGATGCCTTTTCGCGCTGATTAGAAGGTTTTGGTGTTACTAAGATT
GCCATTGAACCAGGGGCACAATTTGACGAAAAGGTGATGGCAGCACTGGAAGTTGTGCCTTCTGATCAACCGGCTAATAC
GGTGGTGAAGGTATCGAAATCGGGTTACAAACTCCACGACAAGGTCATTCGCTTTGCTTCCGTAGTGGTAAGCCAAGGTA
ATAAAACAGAATAA

Upstream 100 bases:

>100_bases
TACCACTCGGCTCTCACTGCCCAGAAAAAAACCTTGGACCGCAAAAGCTATCAATGTTTAGCAGTGGAAAGCCATTAATT
AGAATTACAATAAATTACTC

Downstream 100 bases:

>100_bases
TGAGCACAAATAAAAGAAGAACCATTCAGATTGAAATCACTGAAGAACACTTCAAGGATTTAGAAAAAGCCTTGGAAGCC
TTGAAGGGTACCCAATTACC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSENSLTITEILSSIRTLLVKHNKAKVTQIEKELLQAVAELEKKFKQQVQNFNELQQKIPNLQKVNEEFRLKVEKIQEEA
QKKIQEKVAELTIKSKEELENAKKYVIEKSIDQPLIIIDQFEIALSYAQKDPQVKNYTTGFNMVLDAFSRWLEGFGVTKI
AIEPGAQFDEKVMAALEVVPSDQPANTVVKVSKSGYKLHDKVIRFASVVVSQGNKTE

Sequences:

>Translated_217_residues
MSENSLTITEILSSIRTLLVKHNKAKVTQIEKELLQAVAELEKKFKQQVQNFNELQQKIPNLQKVNEEFRLKVEKIQEEA
QKKIQEKVAELTIKSKEELENAKKYVIEKSIDQPLIIIDQFEIALSYAQKDPQVKNYTTGFNMVLDAFSR*LEGFGVTKI
AIEPGAQFDEKVMAALEVVPSDQPANTVVKVSKSGYKLHDKVIRFASVVVSQGNKTE
>Mature_216_residues
SENSLTITEILSSIRTLLVKHNKAKVTQIEKELLQAVAELEKKFKQQVQNFNELQQKIPNLQKVNEEFRLKVEKIQEEAQ
KKIQEKVAELTIKSKEELENAKKYVIEKSIDQPLIIIDQFEIALSYAQKDPQVKNYTTGFNMVLDAFSR*LEGFGVTKIA
IEPGAQFDEKVMAALEVVPSDQPANTVVKVSKSGYKLHDKVIRFASVVVSQGNKTE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

None

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 24502; Mature: 24370

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENSLTITEILSSIRTLLVKHNKAKVTQIEKELLQAVAELEKKFKQQVQNFNELQQKIP
CCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLQKVNEEFRLKVEKIQEEAQKKIQEKVAELTIKSKEELENAKKYVIEKSIDQPLIIIDQ
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHH
FEIALSYAQKDPQVKNYTTGFNMVLDAFSRLEGFGVTKIAIEPGAQFDEKVMAALEVVPS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCC
DQPANTVVKVSKSGYKLHDKVIRFASVVVSQGNKTE
CCCCHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SENSLTITEILSSIRTLLVKHNKAKVTQIEKELLQAVAELEKKFKQQVQNFNELQQKIP
CCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLQKVNEEFRLKVEKIQEEAQKKIQEKVAELTIKSKEELENAKKYVIEKSIDQPLIIIDQ
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHH
FEIALSYAQKDPQVKNYTTGFNMVLDAFSRLEGFGVTKIAIEPGAQFDEKVMAALEVVPS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCC
DQPANTVVKVSKSGYKLHDKVIRFASVVVSQGNKTE
CCCCHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633; 11271496 [H]