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The map label for this gene is pheS [H]

Identifier: 13507844

GI number: 13507844

Start: 135731

End: 136756

Strand: Direct

Name: pheS [H]

Synonym: MPN105

Alternate gene names: 13507844

Gene position: 135731-136756 (Clockwise)

Preceding gene: 13507843

Following gene: 13507845

Centisome position: 16.63

GC content: 40.16

Gene sequence:

>1026_bases
ATGATTGATCAAAGTAAGTTAATTGAAAGGTGAAAAACTACCTTTGAAACAGCACAAAATCCTACTGAATTATTAGCTTT
TAAAAACAGCTTCCGCAATGCTGATTTAAAGCCTTTACTTAGTCAAATTAAGGAAACCACGGACATCGAAACTAAGCGCC
ATTTAGGCCAACTTTACAAGCAATTGGAAAGCACCTTGCAAACACTCCATGATACACAGCTACAAGTCTTTACCCAAGCC
CAGTCTAGCAGTGTATTAACTCATGGTGATGTGATGTTGCTAGCAACCTCTTTTGCCCCTGGTTCTAGCAACATTATTTA
CCAGGTAATTGATGAGTTAGTGAACTATTTTAAGAAGTTCTTGTTTACGGTTAACTATGACAGTGAATTGACTACTATTG
CAGACTGTTTTGACCTGCTAAACATTCCCAAAGACCATCCTAGTCGTAACCTTACTGACACCTTTTACTTAGACAAAAAC
CGCTTACTGAGAACACATTGTACTGCTGCTACTTTACGTGCTGTTAAGGAAACGAAAAAGAGTAATAACCCCGATATTCG
GATTGCTTCCTTTGGTGCTGTTTTTCGTAAAGATGATGACGATGCCACCCACTCACACCAGTTCAACCAACTGGACTTCA
TGTGGATTAAAAAGGATTTTTCTTTAACTAACCTCAAGTGGTTTATGCAGAATATGATTAACCACATCTTTGGCGAAAAT
ACTTCAGCGCGTTTTCGTCTGTCCCACTTCCCTTTTACCGAACCTTCCTTTGAAATTGACATCCGTTGCTGGTTGTGTCA
GAATGGTTGTGGTGTATGTAAGAAAACACGTTGGATTGAGGTATTAGGGGCGGGTATCCTCCACCCTCAAGTGATGGCTA
ACATGGGCTTTAGTGACACGGATAATATAAGGGGTATAGCAGCTGGCATTGGGATTGAGCGGTTGGTAATGTTGAAACAC
GGCATTAGTGACATCCGTGACCTTTACGACAATAACTTCAAATTTTTAGCCCAGTTTACTGATTAG

Upstream 100 bases:

>100_bases
TAAAAAAGCCAAAAGCTTTTAAAGTAACTATTTATTTGCAAATTAGTGCAAATATTCGTGTGCTATTTAAATTAACTATA
GCACAATTAATAACAGTTGC

Downstream 100 bases:

>100_bases
CGATGTTAATTTCTAAAAAAACTTTAGCTGTTTTAATTCCCGAAATTACCCACGTTTCCAACAATGAAATTTGTGAAAAG
TTACAGCAAATCGGGATTGA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MIDQSKLIERWKTTFETAQNPTELLAFKNSFRNADLKPLLSQIKETTDIETKRHLGQLYKQLESTLQTLHDTQLQVFTQA
QSSSVLTHGDVMLLATSFAPGSSNIIYQVIDELVNYFKKFLFTVNYDSELTTIADCFDLLNIPKDHPSRNLTDTFYLDKN
RLLRTHCTAATLRAVKETKKSNNPDIRIASFGAVFRKDDDDATHSHQFNQLDFMWIKKDFSLTNLKWFMQNMINHIFGEN
TSARFRLSHFPFTEPSFEIDIRCWLCQNGCGVCKKTRWIEVLGAGILHPQVMANMGFSDTDNIRGIAAGIGIERLVMLKH
GISDIRDLYDNNFKFLAQFTD

Sequences:

>Translated_341_residues
MIDQSKLIER*KTTFETAQNPTELLAFKNSFRNADLKPLLSQIKETTDIETKRHLGQLYKQLESTLQTLHDTQLQVFTQA
QSSSVLTHGDVMLLATSFAPGSSNIIYQVIDELVNYFKKFLFTVNYDSELTTIADCFDLLNIPKDHPSRNLTDTFYLDKN
RLLRTHCTAATLRAVKETKKSNNPDIRIASFGAVFRKDDDDATHSHQFNQLDFMWIKKDFSLTNLKWFMQNMINHIFGEN
TSARFRLSHFPFTEPSFEIDIRCWLCQNGCGVCKKTRWIEVLGAGILHPQVMANMGFSDTDNIRGIAAGIGIERLVMLKH
GISDIRDLYDNNFKFLAQFTD
>Mature_341_residues
MIDQSKLIER*KTTFETAQNPTELLAFKNSFRNADLKPLLSQIKETTDIETKRHLGQLYKQLESTLQTLHDTQLQVFTQA
QSSSVLTHGDVMLLATSFAPGSSNIIYQVIDELVNYFKKFLFTVNYDSELTTIADCFDLLNIPKDHPSRNLTDTFYLDKN
RLLRTHCTAATLRAVKETKKSNNPDIRIASFGAVFRKDDDDATHSHQFNQLDFMWIKKDFSLTNLKWFMQNMINHIFGEN
TSARFRLSHFPFTEPSFEIDIRCWLCQNGCGVCKKTRWIEVLGAGILHPQVMANMGFSDTDNIRGIAAGIGIERLVMLKH
GISDIRDLYDNNFKFLAQFTD

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=265, Percent_Identity=30.9433962264151, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI4758340, Length=233, Percent_Identity=28.755364806867, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1788007, Length=250, Percent_Identity=38.4, Blast_Score=181, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI32566635, Length=262, Percent_Identity=29.0076335877863, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI32563657, Length=258, Percent_Identity=26.7441860465116, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17508957, Length=258, Percent_Identity=26.7441860465116, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6321087, Length=232, Percent_Identity=28.0172413793103, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325304, Length=301, Percent_Identity=24.9169435215947, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17137424, Length=287, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18858079, Length=233, Percent_Identity=27.4678111587983, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022911
- InterPro:   IPR002319 [H]

Pfam domain/function: PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39037; Mature: 39037

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDQSKLIERKTTFETAQNPTELLAFKNSFRNADLKPLLSQIKETTDIETKRHLGQLYKQ
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
LESTLQTLHDTQLQVFTQAQSSSVLTHGDVMLLATSFAPGSSNIIYQVIDELVNYFKKFL
HHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
FTVNYDSELTTIADCFDLLNIPKDHPSRNLTDTFYLDKNRLLRTHCTAATLRAVKETKKS
EEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCC
NNPDIRIASFGAVFRKDDDDATHSHQFNQLDFMWIKKDFSLTNLKWFMQNMINHIFGENT
CCCCEEEEECCHHEECCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
SARFRLSHFPFTEPSFEIDIRCWLCQNGCGVCKKTRWIEVLGAGILHPQVMANMGFSDTD
CCEEEEECCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCC
NIRGIAAGIGIERLVMLKHGISDIRDLYDNNFKFLAQFTD
CCCHHHHHCCHHHHHHHHHCHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure
MIDQSKLIERKTTFETAQNPTELLAFKNSFRNADLKPLLSQIKETTDIETKRHLGQLYKQ
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
LESTLQTLHDTQLQVFTQAQSSSVLTHGDVMLLATSFAPGSSNIIYQVIDELVNYFKKFL
HHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
FTVNYDSELTTIADCFDLLNIPKDHPSRNLTDTFYLDKNRLLRTHCTAATLRAVKETKKS
EEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCC
NNPDIRIASFGAVFRKDDDDATHSHQFNQLDFMWIKKDFSLTNLKWFMQNMINHIFGENT
CCCCEEEEECCHHEECCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
SARFRLSHFPFTEPSFEIDIRCWLCQNGCGVCKKTRWIEVLGAGILHPQVMANMGFSDTD
CCEEEEECCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCC
NIRGIAAGIGIERLVMLKHGISDIRDLYDNNFKFLAQFTD
CCCHHHHHCCHHHHHHHHHCHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633; 11271496 [H]