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The map label for this gene is tklB [H]

Identifier: 13507821

GI number: 13507821

Start: 101985

End: 103931

Strand: Direct

Name: tklB [H]

Synonym: MPN082

Alternate gene names: 13507821

Gene position: 101985-103931 (Clockwise)

Preceding gene: 13507820

Following gene: 13507822

Centisome position: 12.49

GC content: 45.04

Gene sequence:

>1947_bases
ATGAAAAACCTGTTTGCTTGCCAACACTTAGCGTTAAGTGCTATTCAACACGCTAAGGGTGGTCATGTGGGAATGGCCTT
AGGTGCTAGTCCTATTTTGTACACCTTATGGACTAAACACATCCAGTTTAACCCTAACTGTCCCAAATGGATTAACCGGG
ACCGTTTAGTGATGAGTGCAGGTCATGGTAGTATGGCCTTATACCCGATCTTACACTTTGCGGGGTTAATTACGAAGCAG
GAAATGCTCCATCACAAATACGGGCAAGTCAACACCTCTTCCCATCCCGAGTATGCCCCTAATAACTTTATTGATGCGTC
CACTGGTCCCTTAGGACAAGGCCTAGGAATGGCTGTCGGGATGGCCTTGACACAACGGGTACTAGCAGCTGAATTTAAAG
CGTTAAGTCCCAAACTGTTTGACCACTTTACCTATGTGGTTGTGGGTGATGGTGATCTCCAGGAAGGGGTGAGTTATGAG
GTAGCTCACTTAGCAGGGGTCTACCAGTTAAATAAGCTAATAGTGCTCCATGATTCCAACCGGGTGCAGATGGACAGTGT
CGTGCGTGATGTGTCCTTGGAAAACCTGCAAACCCGGTTTACCAACATGGGGTGAAATTACCTTGAAACTAGTGATGCAG
TTGCAGACATTGATGCTGCCATTAAACAAGCGAAAAAGTCCGATAAGCCGACCTTCATTGAGGTGCACACGACAATTGCT
AAAAACACCACCTTGGAAGACCAACCCGCTGGTCACTGGTTTATTCCTACCGACAAGGACTTTGCGCGCTTTAACAGCAA
CACCAAGACTAACTTTACCCCGTTTGAATATCCCCAAACGGTGTATGACTTCTTTCACAAGCAGGTCATAGCACGCCAAG
CTAAACCAGTGCAAGCTTACAAGGAATTACTGGAAAAACTAAAGGACAAACCGCTGTATACTAAGTTTATTAATTGAACG
GAAAACGATTACCAGGCGTTGTACCTCAACCAGTTAGATGAACGCAAGGTAGCACAAGCCAATGCGGCAACCCGTAACTA
CCTCAAGGACTTTTTAGGCCAAATTAACAACAGTAATTCCAACTTGTACTGTCTCAATGCTGATGTAGCACGTTCGTGTA
ACATTAAGTTAGGTGATGATAATTTACATACCAATCCGCATTCGCGCAACATTCAGGTGGGTATTCGGGAATTTGGTATG
TCCACCATTATGAACGGGATGGCACTCCATGGTGGGGTCAAAGTGATGGGTGGTACCTTCCTAGCGTTTGCTGATTATTC
CAAACCCGCAATTCGCTTAGGGGCGTTAATGAACCTCCCCACCTTTTATGTGTACACCCATGACTCTTACCAAGTCGGTG
GTGATGGACCAACCCACCAACCGTACGATCAACTCCCGATGTTAAGGGCCATTGAAAACGTTCAGGTTTGACGGCCTTGT
GATGAAAAGGAAACAGCGGCTGGGGTTAACTATGGTTTGTTAAGCCAAGACCAAACTAACGTATTAATTTTGACCCGTCA
AGCTTTGCCTTCCCTAGAACAATCTGATAGTGTACAGACCTTGAAAGGGGGCTATATTATTAGTAATAGAAAACAACCCG
ATGTCATTGTGGCCGCTAGTGGCAGTGAAGTTCAGCTGGCATTGCAACTAGAACAAGCTCTAAACGAACAACAATTAAAG
ACGCGGGTGGTGTCAGTGCCCAACATTAACATGTTGTTAAGTCAACCCCAAAGCTACTTGCAACAACTGTTTGATCCTAA
CAGTGTTTTACTCACCTTAGAAGCGAGTGCTAGCATGGAGTGGTATGCACTAGCAAAATACGTCAAAAAGCACACCCACT
TAGGTGCTTTTAGCTTTGGTGAGTCCAATGACGGGCAAGTCGTGTATGAACACAAGGGCTTTAATGTGACTAACTTACTC
AAGTTGATTAAAACTCTAAAGAGTTAA

Upstream 100 bases:

>100_bases
TTTATGTGGCAGATGGCAAAATTCAAGCACTGCAAGCAAACCCTAATCCCAAACAGGTTGAGGACATTAACTGAATTTAG
ATCTAATAAAATTAGAGCAA

Downstream 100 bases:

>100_bases
AATAGAGTAATGGTTAAGGTCTGAAAAATTGGGTTTGGGGTCTTTCTACCAACAGCGCTTTTATTCTCGGCCTGTTCGTT
TAAGGACTATATTCCTACAC

Product: transketolase

Products: NA

Alternate protein names: TK [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MKNLFACQHLALSAIQHAKGGHVGMALGASPILYTLWTKHIQFNPNCPKWINRDRLVMSAGHGSMALYPILHFAGLITKQ
EMLHHKYGQVNTSSHPEYAPNNFIDASTGPLGQGLGMAVGMALTQRVLAAEFKALSPKLFDHFTYVVVGDGDLQEGVSYE
VAHLAGVYQLNKLIVLHDSNRVQMDSVVRDVSLENLQTRFTNMGWNYLETSDAVADIDAAIKQAKKSDKPTFIEVHTTIA
KNTTLEDQPAGHWFIPTDKDFARFNSNTKTNFTPFEYPQTVYDFFHKQVIARQAKPVQAYKELLEKLKDKPLYTKFINWT
ENDYQALYLNQLDERKVAQANAATRNYLKDFLGQINNSNSNLYCLNADVARSCNIKLGDDNLHTNPHSRNIQVGIREFGM
STIMNGMALHGGVKVMGGTFLAFADYSKPAIRLGALMNLPTFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVQVWRPC
DEKETAAGVNYGLLSQDQTNVLILTRQALPSLEQSDSVQTLKGGYIISNRKQPDVIVAASGSEVQLALQLEQALNEQQLK
TRVVSVPNINMLLSQPQSYLQQLFDPNSVLLTLEASASMEWYALAKYVKKHTHLGAFSFGESNDGQVVYEHKGFNVTNLL
KLIKTLKS

Sequences:

>Translated_648_residues
MKNLFACQHLALSAIQHAKGGHVGMALGASPILYTLWTKHIQFNPNCPKWINRDRLVMSAGHGSMALYPILHFAGLITKQ
EMLHHKYGQVNTSSHPEYAPNNFIDASTGPLGQGLGMAVGMALTQRVLAAEFKALSPKLFDHFTYVVVGDGDLQEGVSYE
VAHLAGVYQLNKLIVLHDSNRVQMDSVVRDVSLENLQTRFTNMG*NYLETSDAVADIDAAIKQAKKSDKPTFIEVHTTIA
KNTTLEDQPAGHWFIPTDKDFARFNSNTKTNFTPFEYPQTVYDFFHKQVIARQAKPVQAYKELLEKLKDKPLYTKFIN*T
ENDYQALYLNQLDERKVAQANAATRNYLKDFLGQINNSNSNLYCLNADVARSCNIKLGDDNLHTNPHSRNIQVGIREFGM
STIMNGMALHGGVKVMGGTFLAFADYSKPAIRLGALMNLPTFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVQV*RPC
DEKETAAGVNYGLLSQDQTNVLILTRQALPSLEQSDSVQTLKGGYIISNRKQPDVIVAASGSEVQLALQLEQALNEQQLK
TRVVSVPNINMLLSQPQSYLQQLFDPNSVLLTLEASASMEWYALAKYVKKHTHLGAFSFGESNDGQVVYEHKGFNVTNLL
KLIKTLKS
>Mature_648_residues
MKNLFACQHLALSAIQHAKGGHVGMALGASPILYTLWTKHIQFNPNCPKWINRDRLVMSAGHGSMALYPILHFAGLITKQ
EMLHHKYGQVNTSSHPEYAPNNFIDASTGPLGQGLGMAVGMALTQRVLAAEFKALSPKLFDHFTYVVVGDGDLQEGVSYE
VAHLAGVYQLNKLIVLHDSNRVQMDSVVRDVSLENLQTRFTNMG*NYLETSDAVADIDAAIKQAKKSDKPTFIEVHTTIA
KNTTLEDQPAGHWFIPTDKDFARFNSNTKTNFTPFEYPQTVYDFFHKQVIARQAKPVQAYKELLEKLKDKPLYTKFIN*T
ENDYQALYLNQLDERKVAQANAATRNYLKDFLGQINNSNSNLYCLNADVARSCNIKLGDDNLHTNPHSRNIQVGIREFGM
STIMNGMALHGGVKVMGGTFLAFADYSKPAIRLGALMNLPTFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVQV*RPC
DEKETAAGVNYGLLSQDQTNVLILTRQALPSLEQSDSVQTLKGGYIISNRKQPDVIVAASGSEVQLALQLEQALNEQQLK
TRVVSVPNINMLLSQPQSYLQQLFDPNSVLLTLEASASMEWYALAKYVKKHTHLGAFSFGESNDGQVVYEHKGFNVTNLL
KLIKTLKS

Specific function: Unknown

COG id: COG0021

COG function: function code G; Transketolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI205277463, Length=570, Percent_Identity=24.5614035087719, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI4507521, Length=570, Percent_Identity=24.5614035087719, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI133778974, Length=569, Percent_Identity=21.9683655536028, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI48994911, Length=663, Percent_Identity=33.3333333333333, Blast_Score=340, Evalue=2e-94,
Organism=Escherichia coli, GI1788808, Length=650, Percent_Identity=32.6153846153846, Blast_Score=324, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17539652, Length=207, Percent_Identity=31.8840579710145, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6325331, Length=645, Percent_Identity=34.5736434108527, Blast_Score=325, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6319593, Length=647, Percent_Identity=34.0030911901082, Blast_Score=313, Evalue=5e-86,
Organism=Drosophila melanogaster, GI45551847, Length=565, Percent_Identity=24.070796460177, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45550715, Length=565, Percent_Identity=24.070796460177, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24645119, Length=543, Percent_Identity=23.9410681399632, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24666278, Length=245, Percent_Identity=31.8367346938775, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005478
- InterPro:   IPR020826
- InterPro:   IPR005474 [H]

Pfam domain/function: PF02779 Transket_pyr; PF00456 Transketolase_N [H]

EC number: =2.2.1.1 [H]

Molecular weight: Translated: 71767; Mature: 71767

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00802 TRANSKETOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLFACQHLALSAIQHAKGGHVGMALGASPILYTLWTKHIQFNPNCPKWINRDRLVMSA
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHEEEEHHHEEECCCCHHHCCCCEEEEEC
GHGSMALYPILHFAGLITKQEMLHHKYGQVNTSSHPEYAPNNFIDASTGPLGQGLGMAVG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
MALTQRVLAAEFKALSPKLFDHFTYVVVGDGDLQEGVSYEVAHLAGVYQLNKLIVLHDSN
HHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCHHCCCCCHHHHHHHHHEECEEEEEECCC
RVQMDSVVRDVSLENLQTRFTNMGNYLETSDAVADIDAAIKQAKKSDKPTFIEVHTTIAK
CEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEC
NTTLEDQPAGHWFIPTDKDFARFNSNTKTNFTPFEYPQTVYDFFHKQVIARQAKPVQAYK
CCCCCCCCCCCEECCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
ELLEKLKDKPLYTKFINTENDYQALYLNQLDERKVAQANAATRNYLKDFLGQINNSNSNL
HHHHHHHCCCCEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
YCLNADVARSCNIKLGDDNLHTNPHSRNIQVGIREFGMSTIMNGMALHGGVKVMGGTFLA
EEEECCCCCCCEEEECCCCCCCCCCCCCEEEHHHHHCHHHHHCCHHHCCCEEEECCEEEE
FADYSKPAIRLGALMNLPTFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVQVRPCDEK
EECCCCCHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCEECCCCCC
ETAAGVNYGLLSQDQTNVLILTRQALPSLEQSDSVQTLKGGYIISNRKQPDVIVAASGSE
HHHCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCEEECCCEEECCCCCCCEEEEECCCC
VQLALQLEQALNEQQLKTRVVSVPNINMLLSQPQSYLQQLFDPNSVLLTLEASASMEWYA
EEEEEEHHHHHCHHHHHHHHCCCCCCHHHHHCHHHHHHHHCCCCEEEEEEECCCCCHHHH
LAKYVKKHTHLGAFSFGESNDGQVVYEHKGFNVTNLLKLIKTLKS
HHHHHHHHCCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKNLFACQHLALSAIQHAKGGHVGMALGASPILYTLWTKHIQFNPNCPKWINRDRLVMSA
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHEEEEHHHEEECCCCHHHCCCCEEEEEC
GHGSMALYPILHFAGLITKQEMLHHKYGQVNTSSHPEYAPNNFIDASTGPLGQGLGMAVG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
MALTQRVLAAEFKALSPKLFDHFTYVVVGDGDLQEGVSYEVAHLAGVYQLNKLIVLHDSN
HHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCHHCCCCCHHHHHHHHHEECEEEEEECCC
RVQMDSVVRDVSLENLQTRFTNMGNYLETSDAVADIDAAIKQAKKSDKPTFIEVHTTIAK
CEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEC
NTTLEDQPAGHWFIPTDKDFARFNSNTKTNFTPFEYPQTVYDFFHKQVIARQAKPVQAYK
CCCCCCCCCCCEECCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
ELLEKLKDKPLYTKFINTENDYQALYLNQLDERKVAQANAATRNYLKDFLGQINNSNSNL
HHHHHHHCCCCEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
YCLNADVARSCNIKLGDDNLHTNPHSRNIQVGIREFGMSTIMNGMALHGGVKVMGGTFLA
EEEECCCCCCCEEEECCCCCCCCCCCCCEEEHHHHHCHHHHHCCHHHCCCEEEECCEEEE
FADYSKPAIRLGALMNLPTFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVQVRPCDEK
EECCCCCHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCEECCCCCC
ETAAGVNYGLLSQDQTNVLILTRQALPSLEQSDSVQTLKGGYIISNRKQPDVIVAASGSE
HHHCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCEEECCCEEECCCCCCCEEEEECCCC
VQLALQLEQALNEQQLKTRVVSVPNINMLLSQPQSYLQQLFDPNSVLLTLEASASMEWYA
EEEEEEHHHHHCHHHHHHHHCCCCCCHHHHHCHHHHHHHHCCCCEEEEEEECCCCCHHHH
LAKYVKKHTHLGAFSFGESNDGQVVYEHKGFNVTNLLKLIKTLKS
HHHHHHHHCCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633; 11271496 [H]