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The map label for this gene is ptsH

Identifier: 13507792

GI number: 13507792

Start: 66822

End: 67088

Strand: Direct

Name: ptsH

Synonym: MPN053

Alternate gene names: 13507792

Gene position: 66822-67088 (Clockwise)

Preceding gene: 13507791

Following gene: 13507793

Centisome position: 8.19

GC content: 38.58

Gene sequence:

>267_bases
ATGAAGAAGATTCAAGTAGTCGTTAAAGACCCTGTGGGCATTCACGCCCGTCCTGCTAGTATTATTGCTGGTGAGGCTAA
TAAATTTAAATCGGAATTAAAGCTAGTATCACCAAGCGGCGTTGAAGGTAACATCAAGTCGATCATCAACTTAATGTCTT
TAGGCATTAAGCAAAACGACCACATTACCATCAAGGCAGAAGGAACTGATGAAGAAGAGGCACTAAATGCTATTAAAGCA
GTTTTAGAAAAACACCAAGTTATTTAA

Upstream 100 bases:

>100_bases
TCCCTACAGAAGCGGTAAGCGTTATTAACGGAAGTACCGCTTTAGACGGCAAAGGTTTTAAGTGGAGCTTTAAACAAATT
TAAAATAATACTCGGCTGAT

Downstream 100 bases:

>100_bases
GTAATTTAATTTAACTTAGAATAAAAGCAACAAGCCAATCCCCAAGGAAAAGCTTGTTGCTTTTTTATTAAGAAATATGC
TTTACTACGACGACGAGGAT

Product: phosphocarrier protein HPr

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; D-glucosamine-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; galactitol-1-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm]; fructose-1-phosphate [Cytoplasm]; glucose-6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm]; salicin-6-phosphate [Cytoplasm]; arbutin-6-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein

Number of amino acids: Translated: 88; Mature: 88

Protein sequence:

>88_residues
MKKIQVVVKDPVGIHARPASIIAGEANKFKSELKLVSPSGVEGNIKSIINLMSLGIKQNDHITIKAEGTDEEEALNAIKA
VLEKHQVI

Sequences:

>Translated_88_residues
MKKIQVVVKDPVGIHARPASIIAGEANKFKSELKLVSPSGVEGNIKSIINLMSLGIKQNDHITIKAEGTDEEEALNAIKA
VLEKHQVI
>Mature_88_residues
MKKIQVVVKDPVGIHARPASIIAGEANKFKSELKLVSPSGVEGNIKSIINLMSLGIKQNDHITIKAEGTDEEEALNAIKA
VLEKHQVI

Specific function: P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbo

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain

Homologues:

None

Paralogues:

None

Copy number: 4180 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 2100 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 3235 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PTHP_MYCPN (P75061)

Other databases:

- EMBL:   U00089
- PIR:   S73427
- RefSeq:   NP_109741.1
- ProteinModelPortal:   P75061
- SMR:   P75061
- GeneID:   876864
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN053
- HOGENOM:   HBG653254
- OMA:   MAMAIKT
- ProtClustDB:   CLSK542113
- BioCyc:   MPNE272634:MPN053-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- Gene3D:   G3DSA:3.30.1340.10
- PRINTS:   PR00107
- TIGRFAMs:   TIGR01003

Pfam domain/function: PF00381 PTS-HPr; SSF55594 HPr_protein

EC number: NA

Molecular weight: Translated: 9495; Mature: 9495

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS51350 PTS_HPR_DOM; PS00369 PTS_HPR_HIS; PS00589 PTS_HPR_SER

Important sites: ACT_SITE 15-15

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIQVVVKDPVGIHARPASIIAGEANKFKSELKLVSPSGVEGNIKSIINLMSLGIKQND
CCCEEEEEECCCCCCCCCCCEEECCCHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCCC
HITIKAEGTDEEEALNAIKAVLEKHQVI
EEEEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIQVVVKDPVGIHARPASIIAGEANKFKSELKLVSPSGVEGNIKSIINLMSLGIKQND
CCCEEEEEECCCCCCCCCCCEEECCCHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCCC
HITIKAEGTDEEEALNAIKAVLEKHQVI
EEEEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sorbitol [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm]; mannitol [Periplasm]; glucosamine [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; galactitol [Periplasm]; fructose [Periplasm]; beta-D-glucose [Periplasm]; diacetylchitobiose [Periplasm]; cellobiose [Periplasm]; salicin [Periplasm]; arbutin [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + mannitol [Periplasm] = mannitol-1-phosp

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633; 11271496