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The map label for this gene is gyrB [H]

Identifier: 13507742

GI number: 13507742

Start: 2869

End: 4821

Strand: Direct

Name: gyrB [H]

Synonym: MPN003

Alternate gene names: 13507742

Gene position: 2869-4821 (Clockwise)

Preceding gene: 13507741

Following gene: 13507743

Centisome position: 0.35

GC content: 45.67

Gene sequence:

>1953_bases
ATGGAAGACAATAACAAAACGCAAGCTTACGATTCCAGTAGCATTAAGATTCTTGAAGGCTTAGAGGCCGTGCGTAAGCG
CCCCGGCATGTACATCGGTTCCACCGGCGAAGAAGGGTTGCACCACATGATCTGGGAAATCATTGATAACTCAATTGACG
AGGCCATGGGTGGCTTTGCGAGTACTGTCAAACTAACCCTTAAAGACAACTTTGTCACCATTGTGGAGGATGATGGCCGG
GGCATTCCCGTTGACATCCATCCTAAGACCAACCGCTCCACCGTTGAAACAGTGTTTACGGTACTCCACGCTGGGGGTAA
GTTTGACAACGATAGCTATAAGGTGTCAGGGGGACTCCACGGTGTGGGTGCCTCGGTTGTTAATGCTTTAAGTTCCTCCT
TTAAGGTATGGGTAGCCAGGGAGCACCAACAGTACTTCCTAGCGTTCCACAACGGTGGGGAAGTGATTGGTGATCTTGTC
AACGAAGGTAAGTGTGACAAGGAACACGGTACCAAGGTTGAGTTTGTCCCGGACTTTACCGTGATGGAAAAGAGTGATTA
CAAACAAACGGTAATTGCTAGTAGGTTGCAACAATTAGCCTTTCTTAACAAAGGGATCCAAATTGACTTTGTTGATGAAC
GCCGTCAAAATCCGCAAAGCTTTTCTTGGAAGTATGATGGTGGCTTAGTCCAATACATCCACCACCTCAACAACGAAAAG
GAACCTTTATTTGAGGACATTATCTTTGGTGAAAAAACCGATACTGTTAAATCAGTTAGCCGTGATGAGAGCTACACAAT
TAAGGTGGAAGTGGCCTTTCAGTACAACAAGACGTATAACCAATCAATCTTTAGTTTTTGTAACAACATTAATACCACTG
AAGGCGGTACCCATGTTGAAGGCTTTCGCAATGCCTTAGTGAAGATCATTAACCGCTTTGCTGTCGAAAACAAGTTCTTA
AAGGAAACGGATGAAAAGATTACCCGCGATGACATCTGTGAAGGGTTAACGGCCATTATCTCAATTAAGCACCCCAACCC
CCAGTATGAGGGTCAAACCAAAAAGAAATTGGGTAACACCGAAGTGCGTCCTCTAGTTAACAGTATTGTTAGTGAAATCT
TTGAGCGCTTTATGTTGGAAAACCCCCAGGAAGCCAACGCCATTATTCGCAAAACGCTGTTAGCGCAAGAAGCACGGCGC
CGTAGTCAGGAAGCACGGGAACTGACCCGCAGGAAATCTCCCTTTGACAGTGGTTCACTCCCGGGTAAGTTAGCAGACTG
TACCACCCGTGATCCCTCAATTAGTGAACTGTACATTGTCGAGGGAGATAGCGCTGGTGGTACGGCTAAAACGGGGCGGG
ACCGTTACTTCCAAGCGATCCTACCATTAAGGGGTAAGATCCTCAATGTGGAAAAGTCCCACTTTGAACAAATCTTTAAT
AATGTGGAAATCTCGGCTTTAGTCATGGCCGTTGGCTGTGGCATTAAACCAGACTTCGAGTTGGAAAAACTGCGGTATAA
CAAGATCATTATCATGACCGATGCTGATGTCGATGGGGCGCACATCCGTACCCTCCTTTTAACCTTCTTCTTTCGCTTTA
TGTATCCCTTGGTGGAACAGGGCAACATTTACATTGCCCAACCCCCACTGTATAAGGTCTCTTACTCTAATAAGGACTTA
TACATGCAAACCGATGTCCAACTAGAGGAGTGGAAGCAACAACACCCTAATCTGAAATACAACTTACAACGCTACAAGGG
TTTAGGGGAAATGGATGCCATCCAACTGTGGGAAACCACGATGGATCCCAAGGTGCGTACCTTACTAAAGGTAACCGTTG
AAGATGCTTCAATTGCTGATAAAGCCTTCTCCTTGTTAATGGGCGATGAAGTTCCCCCAAGACGGGAATTCATCGAACAA
AACGCACGTAACGTAAAGAACATTGATATCTAA

Upstream 100 bases:

>100_bases
ATCGCTTAATTTTTAATGTGGTATAATTGTTTGGATTCGCCATAAATTTTGCTTATGGCTTTTAACTTTCCAACGTGCAC
ATTAAATGGAAGACAATTAA

Downstream 100 bases:

>100_bases
TGGCAAAGCAACAAGATCAAATTGACAAAATTCGTCAGGAATTAGCACAATCAGCAATTAAAAACATCTCCCTCTCCAGT
GAACTAGAACGTTCCTTCAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MEDNNKTQAYDSSSIKILEGLEAVRKRPGMYIGSTGEEGLHHMIWEIIDNSIDEAMGGFASTVKLTLKDNFVTIVEDDGR
GIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKVWVAREHQQYFLAFHNGGEVIGDLV
NEGKCDKEHGTKVEFVPDFTVMEKSDYKQTVIASRLQQLAFLNKGIQIDFVDERRQNPQSFSWKYDGGLVQYIHHLNNEK
EPLFEDIIFGEKTDTVKSVSRDESYTIKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFL
KETDEKITRDDICEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSIVSEIFERFMLENPQEANAIIRKTLLAQEARR
RSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSHFEQIFN
NVEISALVMAVGCGIKPDFELEKLRYNKIIIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIYIAQPPLYKVSYSNKDL
YMQTDVQLEEWKQQHPNLKYNLQRYKGLGEMDAIQLWETTMDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEQ
NARNVKNIDI

Sequences:

>Translated_650_residues
MEDNNKTQAYDSSSIKILEGLEAVRKRPGMYIGSTGEEGLHHMIWEIIDNSIDEAMGGFASTVKLTLKDNFVTIVEDDGR
GIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKVWVAREHQQYFLAFHNGGEVIGDLV
NEGKCDKEHGTKVEFVPDFTVMEKSDYKQTVIASRLQQLAFLNKGIQIDFVDERRQNPQSFSWKYDGGLVQYIHHLNNEK
EPLFEDIIFGEKTDTVKSVSRDESYTIKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFL
KETDEKITRDDICEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSIVSEIFERFMLENPQEANAIIRKTLLAQEARR
RSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSHFEQIFN
NVEISALVMAVGCGIKPDFELEKLRYNKIIIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIYIAQPPLYKVSYSNKDL
YMQTDVQLEEWKQQHPNLKYNLQRYKGLGEMDAIQLWETTMDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEQ
NARNVKNIDI
>Mature_650_residues
MEDNNKTQAYDSSSIKILEGLEAVRKRPGMYIGSTGEEGLHHMIWEIIDNSIDEAMGGFASTVKLTLKDNFVTIVEDDGR
GIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKVWVAREHQQYFLAFHNGGEVIGDLV
NEGKCDKEHGTKVEFVPDFTVMEKSDYKQTVIASRLQQLAFLNKGIQIDFVDERRQNPQSFSWKYDGGLVQYIHHLNNEK
EPLFEDIIFGEKTDTVKSVSRDESYTIKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFL
KETDEKITRDDICEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSIVSEIFERFMLENPQEANAIIRKTLLAQEARR
RSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSHFEQIFN
NVEISALVMAVGCGIKPDFELEKLRYNKIIIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIYIAQPPLYKVSYSNKDL
YMQTDVQLEEWKQQHPNLKYNLQRYKGLGEMDAIQLWETTMDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEQ
NARNVKNIDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=616, Percent_Identity=26.6233766233766, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI19913408, Length=637, Percent_Identity=26.8445839874411, Blast_Score=145, Evalue=9e-35,
Organism=Escherichia coli, GI48994957, Length=572, Percent_Identity=46.5034965034965, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1789408, Length=617, Percent_Identity=39.3841166936791, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17535065, Length=634, Percent_Identity=26.1829652996845, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI212645845, Length=617, Percent_Identity=25.9319286871961, Blast_Score=125, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=176, Percent_Identity=30.6818181818182, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324241, Length=678, Percent_Identity=26.8436578171091, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17136538, Length=693, Percent_Identity=26.4069264069264, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 73811; Mature: 73811

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDNNKTQAYDSSSIKILEGLEAVRKRPGMYIGSTGEEGLHHMIWEIIDNSIDEAMGGFA
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCE
STVKLTLKDNFVTIVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLH
EEEEEEEECCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCC
GVGASVVNALSSSFKVWVAREHQQYFLAFHNGGEVIGDLVNEGKCDKEHGTKVEFVPDFT
HHHHHHHHHHHHCEEEEEEECCCEEEEEECCCCHHHHHHHCCCCCCCCCCCEEEEECCCE
VMEKSDYKQTVIASRLQQLAFLNKGIQIDFVDERRQNPQSFSWKYDGGLVQYIHHLNNEK
EECCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEECCHHHHHHHHHCCCC
EPLFEDIIFGEKTDTVKSVSRDESYTIKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVE
CHHHHHHHCCCCCHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHH
GFRNALVKIINRFAVENKFLKETDEKITRDDICEGLTAIISIKHPNPQYEGQTKKKLGNT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEEECCCCCCCCCCHHHHCCCC
EVRPLVNSIVSEIFERFMLENPQEANAIIRKTLLAQEARRRSQEARELTRRKSPFDSGSL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSHFEQIFN
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHC
NVEISALVMAVGCGIKPDFELEKLRYNKIIIMTDADVDGAHIRTLLLTFFFRFMYPLVEQ
CCCHHHHHHHHCCCCCCCCCHHHEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
GNIYIAQPPLYKVSYSNKDLYMQTDVQLEEWKQQHPNLKYNLQRYKGLGEMDAIQLWETT
CCEEEECCCEEEEEECCCCEEEEECCCHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHC
MDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEQNARNVKNIDI
CCHHHEEHHHEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEDNNKTQAYDSSSIKILEGLEAVRKRPGMYIGSTGEEGLHHMIWEIIDNSIDEAMGGFA
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCE
STVKLTLKDNFVTIVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLH
EEEEEEEECCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCC
GVGASVVNALSSSFKVWVAREHQQYFLAFHNGGEVIGDLVNEGKCDKEHGTKVEFVPDFT
HHHHHHHHHHHHCEEEEEEECCCEEEEEECCCCHHHHHHHCCCCCCCCCCCEEEEECCCE
VMEKSDYKQTVIASRLQQLAFLNKGIQIDFVDERRQNPQSFSWKYDGGLVQYIHHLNNEK
EECCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEECCHHHHHHHHHCCCC
EPLFEDIIFGEKTDTVKSVSRDESYTIKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVE
CHHHHHHHCCCCCHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHH
GFRNALVKIINRFAVENKFLKETDEKITRDDICEGLTAIISIKHPNPQYEGQTKKKLGNT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEEECCCCCCCCCCHHHHCCCC
EVRPLVNSIVSEIFERFMLENPQEANAIIRKTLLAQEARRRSQEARELTRRKSPFDSGSL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSHFEQIFN
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHC
NVEISALVMAVGCGIKPDFELEKLRYNKIIIMTDADVDGAHIRTLLLTFFFRFMYPLVEQ
CCCHHHHHHHHCCCCCCCCCHHHEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
GNIYIAQPPLYKVSYSNKDLYMQTDVQLEEWKQQHPNLKYNLQRYKGLGEMDAIQLWETT
CCEEEECCCEEEEEECCCCEEEEECCCHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHC
MDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEQNARNVKNIDI
CCHHHEEHHHEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8083173; 7569993; 8253680 [H]