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The map label for this gene is dnaN

Identifier: 13507740

GI number: 13507740

Start: 692

End: 1834

Strand: Direct

Name: dnaN

Synonym: MPN001

Alternate gene names: 13507740

Gene position: 692-1834 (Clockwise)

Preceding gene: NA

Following gene: 13507741

Centisome position: 0.08

GC content: 33.86

Gene sequence:

>1143_bases
ATGAAAGTTTTGATTAATAAGAATGAGTTGAACAAAATCCTCAAAAAACTCAACAATGTAATCGTATCTAACAATAAGAT
GAAACCATACCACTCTTATTTATTAATAGAGGCTACAGAAAAGGAAATTAACTTCTATGCTAACAACGAGTACTTTTCTG
CTAAATGTACCTTAGCCGAAAACATTGATGTACTTGAAGAAGGTGAAGTAATTGTTAAAGGCAAAATCTTTAGCGAACTC
ATTAACGGAATTAAAGAAGACATCATTACTATTCAAGAGAAAGATCAAACTCTTTTAGTCAAAACAAAAAAAACAAACAT
TAACCTTAACACGATTGATAAGAAAGAATTCCCCAGAATCCGTTTCAACCAAAACGTTGATTTGAAGGAATTTGATGAAC
TTAAAATCCAACACAGCCTTTTAACTAAAGGACTTAAAAAGATTGCCCATGCTGTTTCTACATTTAGAGAATCCACTAGA
AAATTCAACGGGGTTAACTTCAACGGTTCCAATGGTAAACAAATCTTTTTAGAGGCATCGGATTCTTATAAGCTCTCTGT
TTATGAAATCAAACAAAAAACCGATCCATTTAATTTCATTGTCGAAACTAATCTTTTGAGCTTCATCAATTCTTTTAACC
CTGAAGGTGGTGATTTAATCAGTATCTTCTTCCGCAAAGAACACAAGGATGATTTAAGTACCGAATTACTGATTAAGTTA
GATAACTTCTTAATTAACTACACCTCAATTAACGAAAGCTTTCCGCGGGTAATGCAGTTGTTTGACTTTGAACCAGAAAC
CAAAGTAACCATTCAAAAAAACGAACTTAAAGATGCATTACAAAGAATCTTGACGTTAGCTCAAAACGAGCGCTTCTTTC
TCTGTGACATGCAAGTAACCAACTCCCACCTCAAAATTAATTCCAACGTTCAAAACATTGGTGCATCCTTAGAAGAAGTT
ACTTGCCTTAAGTTCGAAGGTCACAAACTCAACATCGCTGTTAATGCGCTTTCCCTCTTGGAACACATTGACTCATTTGA
TACTGATGAAATTGAGCTTTACTTCCAGGGCAGTAACAAATACTTTTTAATTAGTTCGAACAACGAACCTGAACTTAAAG
AAATCCTAGTTCCTTCCAAGTAA

Upstream 100 bases:

>100_bases
AATTATAGTTATTTTATTAACAAAATTAGTGACATTTGGCCATCCATAATAAATAAAATCAAATAAACAAAACAAAAAGC
AAAAAAACTATAAAAAGGGC

Downstream 100 bases:

>100_bases
CTAATGACGCTTTATGACTTACTAGAGCTGCCCCAAACTGCTACACTACAGGAAATCAAAACAGCTTACAAACGCTTAGC
TAAACGTTATCACCCTGACA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKVLINKNELNKILKKLNNVIVSNNKMKPYHSYLLIEATEKEINFYANNEYFSAKCTLAENIDVLEEGEVIVKGKIFSEL
INGIKEDIITIQEKDQTLLVKTKKTNINLNTIDKKEFPRIRFNQNVDLKEFDELKIQHSLLTKGLKKIAHAVSTFRESTR
KFNGVNFNGSNGKQIFLEASDSYKLSVYEIKQKTDPFNFIVETNLLSFINSFNPEGGDLISIFFRKEHKDDLSTELLIKL
DNFLINYTSINESFPRVMQLFDFEPETKVTIQKNELKDALQRILTLAQNERFFLCDMQVTNSHLKINSNVQNIGASLEEV
TCLKFEGHKLNIAVNALSLLEHIDSFDTDEIELYFQGSNKYFLISSNNEPELKEILVPSK

Sequences:

>Translated_380_residues
MKVLINKNELNKILKKLNNVIVSNNKMKPYHSYLLIEATEKEINFYANNEYFSAKCTLAENIDVLEEGEVIVKGKIFSEL
INGIKEDIITIQEKDQTLLVKTKKTNINLNTIDKKEFPRIRFNQNVDLKEFDELKIQHSLLTKGLKKIAHAVSTFRESTR
KFNGVNFNGSNGKQIFLEASDSYKLSVYEIKQKTDPFNFIVETNLLSFINSFNPEGGDLISIFFRKEHKDDLSTELLIKL
DNFLINYTSINESFPRVMQLFDFEPETKVTIQKNELKDALQRILTLAQNERFFLCDMQVTNSHLKINSNVQNIGASLEEV
TCLKFEGHKLNIAVNALSLLEHIDSFDTDEIELYFQGSNKYFLISSNNEPELKEILVPSK
>Mature_380_residues
MKVLINKNELNKILKKLNNVIVSNNKMKPYHSYLLIEATEKEINFYANNEYFSAKCTLAENIDVLEEGEVIVKGKIFSEL
INGIKEDIITIQEKDQTLLVKTKKTNINLNTIDKKEFPRIRFNQNVDLKEFDELKIQHSLLTKGLKKIAHAVSTFRESTR
KFNGVNFNGSNGKQIFLEASDSYKLSVYEIKQKTDPFNFIVETNLLSFINSFNPEGGDLISIFFRKEHKDDLSTELLIKL
DNFLINYTSINESFPRVMQLFDFEPETKVTIQKNELKDALQRILTLAQNERFFLCDMQVTNSHLKINSNVQNIGASLEEV
TCLKFEGHKLNIAVNALSLLEHIDSFDTDEIELYFQGSNKYFLISSNNEPELKEILVPSK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPO3B_MYCPN (Q50313)

Other databases:

- EMBL:   U34816
- EMBL:   U00089
- PIR:   S62836
- RefSeq:   NP_109689.1
- ProteinModelPortal:   Q50313
- IntAct:   Q50313
- GeneID:   877175
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN001
- HOGENOM:   HBG129976
- OMA:   FYANNEY
- ProtClustDB:   CLSK437066
- BioCyc:   MPNE272634:MPN001-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634
- Gene3D:   G3DSA:3.10.150.10
- SMART:   SM00480
- TIGRFAMs:   TIGR00663

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3

EC number: =2.7.7.7

Molecular weight: Translated: 43857; Mature: 43857

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLINKNELNKILKKLNNVIVSNNKMKPYHSYLLIEATEKEINFYANNEYFSAKCTLAE
CEEEECHHHHHHHHHHHHHEEEECCCCCCCCEEEEEEEECCEEEEEECCEEEEEEEEEEC
NIDVLEEGEVIVKGKIFSELINGIKEDIITIQEKDQTLLVKTKKTNINLNTIDKKEFPRI
CCCEECCCCEEEEHHHHHHHHHHHHHHEEEEECCCCEEEEEEEECEEEEEECCCCCCCCE
RFNQNVDLKEFDELKIQHSLLTKGLKKIAHAVSTFRESTRKFNGVNFNGSNGKQIFLEAS
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEC
DSYKLSVYEIKQKTDPFNFIVETNLLSFINSFNPEGGDLISIFFRKEHKDDLSTELLIKL
CCEEEEEEEECCCCCCEEEEEHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHEEEE
DNFLINYTSINESFPRVMQLFDFEPETKVTIQKNELKDALQRILTLAQNERFFLCDMQVT
ECEEEEEECCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEEEC
NSHLKINSNVQNIGASLEEVTCLKFEGHKLNIAVNALSLLEHIDSFDTDEIELYFQGSNK
CCEEEECCCHHHHCCCHHHEEEEEECCCEEEEEHHHHHHHHHHCCCCCCEEEEEEECCCE
YFLISSNNEPELKEILVPSK
EEEEECCCCCCHHHHCCCCC
>Mature Secondary Structure
MKVLINKNELNKILKKLNNVIVSNNKMKPYHSYLLIEATEKEINFYANNEYFSAKCTLAE
CEEEECHHHHHHHHHHHHHEEEECCCCCCCCEEEEEEEECCEEEEEECCEEEEEEEEEEC
NIDVLEEGEVIVKGKIFSELINGIKEDIITIQEKDQTLLVKTKKTNINLNTIDKKEFPRI
CCCEECCCCEEEEHHHHHHHHHHHHHHEEEEECCCCEEEEEEEECEEEEEECCCCCCCCE
RFNQNVDLKEFDELKIQHSLLTKGLKKIAHAVSTFRESTRKFNGVNFNGSNGKQIFLEAS
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEC
DSYKLSVYEIKQKTDPFNFIVETNLLSFINSFNPEGGDLISIFFRKEHKDDLSTELLIKL
CCEEEEEEEECCCCCCEEEEEHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHEEEE
DNFLINYTSINESFPRVMQLFDFEPETKVTIQKNELKDALQRILTLAQNERFFLCDMQVT
ECEEEEEECCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEEEC
NSHLKINSNVQNIGASLEEVTCLKFEGHKLNIAVNALSLLEHIDSFDTDEIELYFQGSNK
CCEEEECCCHHHHCCCHHHEEEEEECCCEEEEEHHHHHHHHHHCCCCCCEEEEEEECCCE
YFLISSNNEPELKEILVPSK
EEEEECCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8604303; 8948633