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The map label for this gene is pdxT

Identifier: 15669857

GI number: 15669857

Start: 1643519

End: 1644079

Strand: Reverse

Name: pdxT

Synonym: MJ1661

Alternate gene names: 15669857

Gene position: 1644079-1643519 (Counterclockwise)

Preceding gene: 15669858

Following gene: 15669847

Centisome position: 98.75

GC content: 32.8

Gene sequence:

>561_bases
ATGATTATTGGTGTCTTAGCAATTCAGGGAGATGTTGAAGAGCATGAGGAAGCTATTAAAAAAGCTGGTTATGAGGCAAA
GAAAGTTAAAAGAGTAGAGGATTTAGAAGGAATTGATGCCTTAATAATTCCAGGAGGGGAGAGCACAGCTATAGGCAAAT
TAATGAAAAAGTATGGATTATTAGAAAAAATAAAAAATTCTAATTTGCCAATATTGGGAACTTGTGCTGGAATGGTTTTG
TTATCAAAAGGGACTGGAATTAATCAAATTCTACTGGAATTGATGGACATTACAGTTAAAAGAAACGCCTATGGAAGGCA
GGTAGATAGCTTTGAAAAAGAAATTGAATTTAAAGATTTAGGAAAGGTTTATGGAGTATTTATAAGAGCCCCAGTGGTTG
ATAAGATTTTAAGTGATGATGTTGAGGTTATAGCAAGAGATGGAGATAAAATTGTTGGTGTTAAGCAAGGAAAATATATG
GCTCTATCATTCCATCCAGAGCTATCTGAAGATGGATATAAGGTTTATAAGTATTTTGTTGAAAACTGTGTTAAAAAATA
A

Upstream 100 bases:

>100_bases
TAAGAAGAAGTAACGCAAGGTTTATGAAGGAAGAATTGAAATGCAAAAATTAAAATAATTTTAAACAATATCTATCGGCT
AAATTTCAAAGAGGGATAAT

Downstream 100 bases:

>100_bases
AAGATTAAAAGATTATTTCTATTTTATCTCAACCTTTGCATTTAAGAAAACTGGTCCCCTAACATCTATAACCTTATTCT
CCCCCATTATCTGTTTTAAA

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MIIGVLAIQGDVEEHEEAIKKAGYEAKKVKRVEDLEGIDALIIPGGESTAIGKLMKKYGLLEKIKNSNLPILGTCAGMVL
LSKGTGINQILLELMDITVKRNAYGRQVDSFEKEIEFKDLGKVYGVFIRAPVVDKILSDDVEVIARDGDKIVGVKQGKYM
ALSFHPELSEDGYKVYKYFVENCVKK

Sequences:

>Translated_186_residues
MIIGVLAIQGDVEEHEEAIKKAGYEAKKVKRVEDLEGIDALIIPGGESTAIGKLMKKYGLLEKIKNSNLPILGTCAGMVL
LSKGTGINQILLELMDITVKRNAYGRQVDSFEKEIEFKDLGKVYGVFIRAPVVDKILSDDVEVIARDGDKIVGVKQGKYM
ALSFHPELSEDGYKVYKYFVENCVKK
>Mature_186_residues
MIIGVLAIQGDVEEHEEAIKKAGYEAKKVKRVEDLEGIDALIIPGGESTAIGKLMKKYGLLEKIKNSNLPILGTCAGMVL
LSKGTGINQILLELMDITVKRNAYGRQVDSFEKEIEFKDLGKVYGVFIRAPVVDKILSDDVEVIARDGDKIVGVKQGKYM
ALSFHPELSEDGYKVYKYFVENCVKK

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=211, Percent_Identity=34.1232227488152, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323995, Length=216, Percent_Identity=35.1851851851852, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321048, Length=216, Percent_Identity=34.7222222222222, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_METJA (Q59055)

Other databases:

- EMBL:   L77117
- PIR:   C64507
- RefSeq:   NP_248671.1
- PDB:   2YWJ
- PDBsum:   2YWJ
- ProteinModelPortal:   Q59055
- SMR:   Q59055
- GeneID:   1452570
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1661
- NMPDR:   fig|243232.1.peg.1708
- TIGR:   MJ1661
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13527
- BioCyc:   MJAN243232:MJ_1661-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 20597; Mature: 20597

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 75-75 ACT_SITE 165-165 ACT_SITE 167-167 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGVLAIQGDVEEHEEAIKKAGYEAKKVKRVEDLEGIDALIIPGGESTAIGKLMKKYGL
CEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHH
LEKIKNSNLPILGTCAGMVLLSKGTGINQILLELMDITVKRNAYGRQVDSFEKEIEFKDL
HHHHCCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHH
GKVYGVFIRAPVVDKILSDDVEVIARDGDKIVGVKQGKYMALSFHPELSEDGYKVYKYFV
HHHHHHHHHHHHHHHHHCCCHHEEECCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHH
ENCVKK
HHHHCC
>Mature Secondary Structure
MIIGVLAIQGDVEEHEEAIKKAGYEAKKVKRVEDLEGIDALIIPGGESTAIGKLMKKYGL
CEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHH
LEKIKNSNLPILGTCAGMVLLSKGTGINQILLELMDITVKRNAYGRQVDSFEKEIEFKDL
HHHHCCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHH
GKVYGVFIRAPVVDKILSDDVEVIARDGDKIVGVKQGKYMALSFHPELSEDGYKVYKYFV
HHHHHHHHHHHHHHHHHCCCHHEEECCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHH
ENCVKK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087