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The map label for this gene is birA [C]

Identifier: 15669815

GI number: 15669815

Start: 1593776

End: 1594489

Strand: Reverse

Name: birA [C]

Synonym: MJ1619

Alternate gene names: 15669815

Gene position: 1594489-1593776 (Counterclockwise)

Preceding gene: 15669817

Following gene: 15669814

Centisome position: 95.77

GC content: 28.01

Gene sequence:

>714_bases
ATGGAAATTATACATTTAAGTGAAATTGATTCAACAAACGACTATGCCAAAGAGTTAGCAAAAGAAGGGAAAAGGAATTT
TATTGTGTTGGCTGATAAACAAAATAATGGGAAAGGAAGATGGGGAAGAGTTTGGTATTCTGATGAGGGAGGATTATATT
TCTCAATGGTCTTAGATTCTAAACTATATAATCCAAAAGTTATCAATTTATTAGTCCCTATTTGTATTATTGAGGTATTA
AAAAACTATGTAGATAAAGAACTTGGTTTAAAGTTTCCAAATGATATAATGGTTAAAGTAAATGATAATTATAAAAAGCT
TGGGGGAATATTAACTGAACTAACTGATGATTACATGATTATAGGAATTGGAATAAATGTAAATAACCAGATAAGAAATG
AGATTAGAGAAATAGCAATCTCTTTAAAAGAAATTACTGGGAAAGAACTTGATAAGGTAGAGATACTTAGCAATTTTCTA
AAAACCTTTGAAAGCTACTTAGAAAAACTTAAAAATAAAGAAATAGATGACTACGAAATATTAAAAAAATATAAAAAATA
CTCAATAACCATTGGAAAGCAGGTAAAAATCCTCTTATCAAACAATGAAATTATTACAGGAAAAGTTTATGATATAGACT
TTGATGGCATTGTCTTAGGAACTGAAAAAGGCATTGAAAGAATCCCTTCTGGAATTTGCATCCATGTAAGATAA

Upstream 100 bases:

>100_bases
AAACAATCAATAATTAATGCTTTCATTTTCTCACTTAACCCATAAATATCTAAACTAACAAAATATTATTTAGGATGATA
TTTAAATTGGGTGGAAATTT

Downstream 100 bases:

>100_bases
AAATTTTTGGTTGATAACCATGAAAATAATAAAAACTGAATATGACAAAATTAAGCCTTATATTACTAAAGATGGCTCAA
TAATTAGAGAATTACTGCAT

Product: biotin operon repressor/biotin--[acetyl-CoA-carboxylase] ligase BirA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MEIIHLSEIDSTNDYAKELAKEGKRNFIVLADKQNNGKGRWGRVWYSDEGGLYFSMVLDSKLYNPKVINLLVPICIIEVL
KNYVDKELGLKFPNDIMVKVNDNYKKLGGILTELTDDYMIIGIGINVNNQIRNEIREIAISLKEITGKELDKVEILSNFL
KTFESYLEKLKNKEIDDYEILKKYKKYSITIGKQVKILLSNNEIITGKVYDIDFDGIVLGTEKGIERIPSGICIHVR

Sequences:

>Translated_237_residues
MEIIHLSEIDSTNDYAKELAKEGKRNFIVLADKQNNGKGRWGRVWYSDEGGLYFSMVLDSKLYNPKVINLLVPICIIEVL
KNYVDKELGLKFPNDIMVKVNDNYKKLGGILTELTDDYMIIGIGINVNNQIRNEIREIAISLKEITGKELDKVEILSNFL
KTFESYLEKLKNKEIDDYEILKKYKKYSITIGKQVKILLSNNEIITGKVYDIDFDGIVLGTEKGIERIPSGICIHVR
>Mature_237_residues
MEIIHLSEIDSTNDYAKELAKEGKRNFIVLADKQNNGKGRWGRVWYSDEGGLYFSMVLDSKLYNPKVINLLVPICIIEVL
KNYVDKELGLKFPNDIMVKVNDNYKKLGGILTELTDDYMIIGIGINVNNQIRNEIREIAISLKEITGKELDKVEILSNFL
KTFESYLEKLKNKEIDDYEILKKYKKYSITIGKQVKILLSNNEIITGKVYDIDFDGIVLGTEKGIERIPSGICIHVR

Specific function: Bira Acts Both As A Biotin-Operon Repressor And As The Enzyme That Synthesizes The Corepressor, Acetyl-CoA:Carbon- Dioxide Ligase. This Protein Also Activates Biotin To Form Biotinyl-5'-Adenylate And Transfers The Biotin Moiety To Biotin-Accepting Protei

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family

Homologues:

Organism=Escherichia coli, GI1790408, Length=242, Percent_Identity=30.1652892561983, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BPL_METJA (Q59014)

Other databases:

- EMBL:   L77117
- PIR:   B64502
- RefSeq:   NP_248629.1
- PDB:   2EJ9
- PDBsum:   2EJ9
- ProteinModelPortal:   Q59014
- SMR:   Q59014
- GeneID:   1452528
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1619
- NMPDR:   fig|243232.1.peg.1666
- TIGR:   MJ1619
- HOGENOM:   HBG750923
- OMA:   GLNINES
- ProtClustDB:   CLSK2750018
- BioCyc:   MJAN243232:MJ_1619-MONOMER
- BRENDA:   6.3.4.15
- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143
- PANTHER:   PTHR12835
- TIGRFAMs:   TIGR00121

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB

EC number: =6.3.4.15

Molecular weight: Translated: 27185; Mature: 27185

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIHLSEIDSTNDYAKELAKEGKRNFIVLADKQNNGKGRWGRVWYSDEGGLYFSMVLDS
CEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEEEEECC
KLYNPKVINLLVPICIIEVLKNYVDKELGLKFPNDIMVKVNDNYKKLGGILTELTDDYMI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEE
IGIGINVNNQIRNEIREIAISLKEITGKELDKVEILSNFLKTFESYLEKLKNKEIDDYEI
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LKKYKKYSITIGKQVKILLSNNEIITGKVYDIDFDGIVLGTEKGIERIPSGICIHVR
HHHHHHHEEEECCEEEEEEECCEEEEEEEEEECCCCEEEECHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MEIIHLSEIDSTNDYAKELAKEGKRNFIVLADKQNNGKGRWGRVWYSDEGGLYFSMVLDS
CEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEEEEECC
KLYNPKVINLLVPICIIEVLKNYVDKELGLKFPNDIMVKVNDNYKKLGGILTELTDDYMI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEE
IGIGINVNNQIRNEIREIAISLKEITGKELDKVEILSNFLKTFESYLEKLKNKEIDDYEI
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LKKYKKYSITIGKQVKILLSNNEIITGKVYDIDFDGIVLGTEKGIERIPSGICIHVR
HHHHHHHEEEECCEEEEEEECCEEEEEEEEEECCCCEEEECHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087