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The map label for this gene is aksF [H]

Identifier: 15669791

GI number: 15669791

Start: 1567330

End: 1568373

Strand: Reverse

Name: aksF [H]

Synonym: MJ1596

Alternate gene names: 15669791

Gene position: 1568373-1567330 (Counterclockwise)

Preceding gene: 15669793

Following gene: 15669790

Centisome position: 94.2

GC content: 31.7

Gene sequence:

>1044_bases
ATGATGAAGGTGTGTGTTATAGAAGGGGATGGAATAGGAAAAGAAGTGATTCCAGAGGCCATAAAAATATTAAATGAGTT
GGGAGAGTTTGAAATAATAAAAGGAGAGGCAGGATTAGAATGTTTAAAAAAATATGGTAATGCACTTCCAGAGGATACAA
TAGAAAAAGCTAAAGAGGCAGATATTATTTTGTTTGGGGCTATAACCTCACCAAAGCCAGGGGAAGTTCAAAATTATAAA
AGCCCTATAATAACGTTGAGGAAGATGTTTCATTTATATGCAAATGTAAGACCAATAAACAACTTTGGAATTGGACAATT
AATTGGGAAAATTGCAGATTATGAATTCTTAAATGCTAAGAATATTGATATAGTTATTATAAGAGAGAATACGGAAGATT
TATATGTTGGTAGAGAGAGATTAGAAAATGATACAGCAATAGCTGAGAGGGTTATAACAAGAAAGGGTAGCGAGAGAATA
ATAAGATTTGCATTTGAATATGCTATAAAAAATAATAGGAAAAAGGTATCTTGCATCCATAAAGCTAATGTTTTAAGAAT
AACTGATGGTTTATTCTTAGAGGTTTTTAATGAAATAAAAAAACATTATAATATAGAGGCAGATGATTATTTAGTTGATT
CAACAGCTATGAACTTAATAAAACATCCTGAAAAATTTGATGTTATTGTTACAACAAACATGTTTGGGGATATTTTATCA
GATGAGGCATCTGCATTAATTGGAGGACTTGGTTTAGCTCCTTCAGCAAATATAGGAGATGATAAAGCATTATTTGAGCC
AGTTCATGGTTCAGCTCCAGATATAGCTGGGAAAGGTATAGCAAATCCAATGGCATCTATATTAAGTATTGCTATGCTTT
TTGATTATATTGGAGAGAAAGAAAAGGGAGATTTGATTAGAGAGGCAGTGAAATACTGCTTAATAAACAAAAAAGTTACT
CCTGACTTGGGAGGGGATTTAAAGACAAAAGATGTTGGAGACGAAATTCTAAATTACATTAGAAAGAAGTTAAAGGGATA
TTGA

Upstream 100 bases:

>100_bases
TAAATATTTAAATGCCTTCCTTTGGAAGGTGTTCATAAGCTACTGGTTACTTCAAAATGTTTTTGAAAGACACTATATAA
AAAAGTTTTTGGTGCTGTCT

Downstream 100 bases:

>100_bases
TGAAAATAAAGATAACTAAATCCACAATATTGTTGATAATTAGCTTTTTATTTATTTTAGCAATAATGGCATATATTGGC
TTAGATAAAATAATAAAAGT

Product: 3-isopropylmalate dehydrogenase LeuB2

Products: NA

Alternate protein names: HICDH; Isohomocitrate dehydrogenase; IHDH; NAD-dependent threo-isohomocitrate dehydrogenase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MMKVCVIEGDGIGKEVIPEAIKILNELGEFEIIKGEAGLECLKKYGNALPEDTIEKAKEADIILFGAITSPKPGEVQNYK
SPIITLRKMFHLYANVRPINNFGIGQLIGKIADYEFLNAKNIDIVIIRENTEDLYVGRERLENDTAIAERVITRKGSERI
IRFAFEYAIKNNRKKVSCIHKANVLRITDGLFLEVFNEIKKHYNIEADDYLVDSTAMNLIKHPEKFDVIVTTNMFGDILS
DEASALIGGLGLAPSANIGDDKALFEPVHGSAPDIAGKGIANPMASILSIAMLFDYIGEKEKGDLIREAVKYCLINKKVT
PDLGGDLKTKDVGDEILNYIRKKLKGY

Sequences:

>Translated_347_residues
MMKVCVIEGDGIGKEVIPEAIKILNELGEFEIIKGEAGLECLKKYGNALPEDTIEKAKEADIILFGAITSPKPGEVQNYK
SPIITLRKMFHLYANVRPINNFGIGQLIGKIADYEFLNAKNIDIVIIRENTEDLYVGRERLENDTAIAERVITRKGSERI
IRFAFEYAIKNNRKKVSCIHKANVLRITDGLFLEVFNEIKKHYNIEADDYLVDSTAMNLIKHPEKFDVIVTTNMFGDILS
DEASALIGGLGLAPSANIGDDKALFEPVHGSAPDIAGKGIANPMASILSIAMLFDYIGEKEKGDLIREAVKYCLINKKVT
PDLGGDLKTKDVGDEILNYIRKKLKGY
>Mature_347_residues
MMKVCVIEGDGIGKEVIPEAIKILNELGEFEIIKGEAGLECLKKYGNALPEDTIEKAKEADIILFGAITSPKPGEVQNYK
SPIITLRKMFHLYANVRPINNFGIGQLIGKIADYEFLNAKNIDIVIIRENTEDLYVGRERLENDTAIAERVITRKGSERI
IRFAFEYAIKNNRKKVSCIHKANVLRITDGLFLEVFNEIKKHYNIEADDYLVDSTAMNLIKHPEKFDVIVTTNMFGDILS
DEASALIGGLGLAPSANIGDDKALFEPVHGSAPDIAGKGIANPMASILSIAMLFDYIGEKEKGDLIREAVKYCLINKKVT
PDLGGDLKTKDVGDEILNYIRKKLKGY

Specific function: Catalyzes the NAD-dependent oxidation and decarboxylation of (2R,3S)-homoisocitrate ((1R,2S)-1- hydroxybutane-1,2,4-tricarboxylate), (2R,3S)-homo(2)-isocitrate and (2R,3S)-homo(3)-isocitrate, into 2-oxoadipate, 2-oxopimelate, and 2-oxosuberate, respective

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=345, Percent_Identity=37.6811594202899, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI4758582, Length=346, Percent_Identity=33.5260115606936, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI28178821, Length=352, Percent_Identity=31.5340909090909, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI28178816, Length=346, Percent_Identity=31.5028901734104, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI28178838, Length=323, Percent_Identity=33.7461300309598, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI28178819, Length=198, Percent_Identity=34.8484848484849, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1788101, Length=366, Percent_Identity=36.8852459016393, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=36.0335195530726, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1787381, Length=396, Percent_Identity=29.5454545454545, Blast_Score=165, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI71986051, Length=328, Percent_Identity=37.1951219512195, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI25144293, Length=355, Percent_Identity=33.8028169014084, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17550882, Length=354, Percent_Identity=34.7457627118644, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17505779, Length=345, Percent_Identity=33.9130434782609, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322097, Length=361, Percent_Identity=40.4432132963989, Blast_Score=245, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6324709, Length=342, Percent_Identity=35.9649122807018, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324291, Length=347, Percent_Identity=35.7348703170029, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6319830, Length=362, Percent_Identity=34.8066298342541, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24643270, Length=345, Percent_Identity=38.2608695652174, Blast_Score=211, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24643268, Length=345, Percent_Identity=38.2608695652174, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24661184, Length=349, Percent_Identity=35.8166189111748, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI161078633, Length=348, Percent_Identity=34.7701149425287, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24650122, Length=348, Percent_Identity=34.7701149425287, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI161078637, Length=348, Percent_Identity=34.7701149425287, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI161078635, Length=348, Percent_Identity=34.7701149425287, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI161078639, Length=346, Percent_Identity=34.6820809248555, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI281362242, Length=342, Percent_Identity=34.2105263157895, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24648872, Length=342, Percent_Identity=34.2105263157895, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI20130355, Length=343, Percent_Identity=27.6967930029155, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550436, Length=230, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.87 [H]

Molecular weight: Translated: 38536; Mature: 38536

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKVCVIEGDGIGKEVIPEAIKILNELGEFEIIKGEAGLECLKKYGNALPEDTIEKAKEA
CEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHCCC
DIILFGAITSPKPGEVQNYKSPIITLRKMFHLYANVRPINNFGIGQLIGKIADYEFLNAK
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEECCC
NIDIVIIRENTEDLYVGRERLENDTAIAERVITRKGSERIIRFAFEYAIKNNRKKVSCIH
CCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEHHH
KANVLRITDGLFLEVFNEIKKHYNIEADDYLVDSTAMNLIKHPEKFDVIVTTNMFGDILS
HCCEEEECCHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHCCCCCEEEEECHHHHHHHH
DEASALIGGLGLAPSANIGDDKALFEPVHGSAPDIAGKGIANPMASILSIAMLFDYIGEK
HHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
EKGDLIREAVKYCLINKKVTPDLGGDLKTKDVGDEILNYIRKKLKGY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMKVCVIEGDGIGKEVIPEAIKILNELGEFEIIKGEAGLECLKKYGNALPEDTIEKAKEA
CEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHCCC
DIILFGAITSPKPGEVQNYKSPIITLRKMFHLYANVRPINNFGIGQLIGKIADYEFLNAK
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEECCC
NIDIVIIRENTEDLYVGRERLENDTAIAERVITRKGSERIIRFAFEYAIKNNRKKVSCIH
CCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEHHH
KANVLRITDGLFLEVFNEIKKHYNIEADDYLVDSTAMNLIKHPEKFDVIVTTNMFGDILS
HCCEEEECCHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHCCCCCEEEEECHHHHHHHH
DEASALIGGLGLAPSANIGDDKALFEPVHGSAPDIAGKGIANPMASILSIAMLFDYIGEK
HHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
EKGDLIREAVKYCLINKKVTPDLGGDLKTKDVGDEILNYIRKKLKGY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10940051 [H]