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The map label for this gene is tpiA

Identifier: 15669723

GI number: 15669723

Start: 1505740

End: 1506399

Strand: Reverse

Name: tpiA

Synonym: MJ1528

Alternate gene names: 15669723

Gene position: 1506399-1505740 (Counterclockwise)

Preceding gene: 15669728

Following gene: 15669722

Centisome position: 90.48

GC content: 34.39

Gene sequence:

>660_bases
ATGTTGATAGTAATTAACTATAAAACATACAATGAAAGTATAGGAAATAGAGGTTTAGAGATAGCTAAAATTGCTGAGAA
AGTTAGTGAAGAAAGTGGAATTACAATAGGAGTAGCTCCTCAATTTGTAGATTTAAGGATGATTGTTGAAAATGTCAATA
TTCCAGTTTATGCTCAACATATAGATAATATAAACCCTGGAAGTCATACTGGACATATATTGGCTGAAGCTATTAAAGAT
TGTGGTTGTAAAGGAACTCTAATAAACCATTCGGAGAAGAGAATGCTGTTGGCTGATATTGAAGCAGTTATAAATAAATG
CAAAAATTTAGGATTAGAAACAATTGTCTGCACAAATAATATAAACACTTCTAAGGCAGTTGCAGCCCTAAGCCCTGATT
ATATTGCTGTTGAACCACCAGAGCTTATAGGAACTGGAATTCCAGTATCAAAGGCAAATCCAGAGGTTGTTGAGGGAACT
GTTAGGGCAGTTAAAGAGATAAACAAGGATGTCAAAGTTTTATGTGGAGCTGGAATTTCTAAAGGAGAAGATGTTAAAGC
AGCCCTTGATTTGGGAGCTGAGGGTGTTTTATTAGCTTCTGGAGTAGTTAAAGCAAAGAATGTAGAAGAGGCTATAAGAG
AATTAATAAAGTTCATCTAA

Upstream 100 bases:

>100_bases
TCTTAAAGGGATTGTATTTCTAACCAATTGATTACCTTTAAATATCATCCCTATAAAATACTCTTCTATCTGAATAATCA
TGCTCTCACGGTGAAAGCCT

Downstream 100 bases:

>100_bases
GTTATAATTTATTTTTCAAAACTTAATAAGATTCTAAGGAAACTTTGAACATTTATCTTATAAGTAAGGTGTTCATTAAG
TATCATTTATTCCAAAATAT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MLIVINYKTYNESIGNRGLEIAKIAEKVSEESGITIGVAPQFVDLRMIVENVNIPVYAQHIDNINPGSHTGHILAEAIKD
CGCKGTLINHSEKRMLLADIEAVINKCKNLGLETIVCTNNINTSKAVAALSPDYIAVEPPELIGTGIPVSKANPEVVEGT
VRAVKEINKDVKVLCGAGISKGEDVKAALDLGAEGVLLASGVVKAKNVEEAIRELIKFI

Sequences:

>Translated_219_residues
MLIVINYKTYNESIGNRGLEIAKIAEKVSEESGITIGVAPQFVDLRMIVENVNIPVYAQHIDNINPGSHTGHILAEAIKD
CGCKGTLINHSEKRMLLADIEAVINKCKNLGLETIVCTNNINTSKAVAALSPDYIAVEPPELIGTGIPVSKANPEVVEGT
VRAVKEINKDVKVLCGAGISKGEDVKAALDLGAEGVLLASGVVKAKNVEEAIRELIKFI
>Mature_219_residues
MLIVINYKTYNESIGNRGLEIAKIAEKVSEESGITIGVAPQFVDLRMIVENVNIPVYAQHIDNINPGSHTGHILAEAIKD
CGCKGTLINHSEKRMLLADIEAVINKCKNLGLETIVCTNNINTSKAVAALSPDYIAVEPPELIGTGIPVSKANPEVVEGT
VRAVKEINKDVKVLCGAGISKGEDVKAALDLGAEGVLLASGVVKAKNVEEAIRELIKFI

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

None

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_METJA (Q58923)

Other databases:

- EMBL:   L77117
- PIR:   G64490
- RefSeq:   NP_248536.1
- PDB:   2H6R
- PDBsum:   2H6R
- ProteinModelPortal:   Q58923
- SMR:   Q58923
- GeneID:   1452436
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1528
- NMPDR:   fig|243232.1.peg.1573
- TIGR:   MJ1528
- HOGENOM:   HBG297256
- OMA:   VEPPELI
- ProtClustDB:   PRK04302
- BioCyc:   MetaCyc:MONOMER-14589
- BioCyc:   MJAN243232:MJ_1528-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_A
- InterPro:   IPR013785
- InterPro:   IPR000652
- InterPro:   IPR022891
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 23319; Mature: 23319

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 90-90 ACT_SITE 138-138 BINDING 6-6 BINDING 8-8

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIVINYKTYNESIGNRGLEIAKIAEKVSEESGITIGVAPQFVDLRMIVENVNIPVYAQH
CEEEEEECHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCCEEEEE
IDNINPGSHTGHILAEAIKDCGCKGTLINHSEKRMLLADIEAVINKCKNLGLETIVCTNN
CCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
INTSKAVAALSPDYIAVEPPELIGTGIPVSKANPEVVEGTVRAVKEINKDVKVLCGAGIS
CCCHHEEEECCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
KGEDVKAALDLGAEGVLLASGVVKAKNVEEAIRELIKFI
CCCCHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MLIVINYKTYNESIGNRGLEIAKIAEKVSEESGITIGVAPQFVDLRMIVENVNIPVYAQH
CEEEEEECHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCCEEEEE
IDNINPGSHTGHILAEAIKDCGCKGTLINHSEKRMLLADIEAVINKCKNLGLETIVCTNN
CCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
INTSKAVAALSPDYIAVEPPELIGTGIPVSKANPEVVEGTVRAVKEINKDVKVLCGAGIS
CCCHHEEEECCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
KGEDVKAALDLGAEGVLLASGVVKAKNVEEAIRELIKFI
CCCCHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087