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The map label for this gene is purT [H]

Identifier: 15669679

GI number: 15669679

Start: 1457720

End: 1458901

Strand: Reverse

Name: purT [H]

Synonym: MJ1486

Alternate gene names: 15669679

Gene position: 1458901-1457720 (Counterclockwise)

Preceding gene: 15669688

Following gene: 15669672

Centisome position: 87.62

GC content: 36.38

Gene sequence:

>1182_bases
TTGGTGAAATTTATGGCAATTGGGACACCTCTTTTGAAAGGAAGTATAAAATTTTTGTTGTTAGGAAGTGGAGAGTTAGG
GAAAGAAGTTGTTATTGAAGCTCAGAGATTGGGAATTGAGTGTATAGCTGTTGATAGGTATCAAAACGCCCCAGCTATGC
AGGTTGCTCACAAGAGCTATGTTATTGATATGAAAGATTACGATGCATTGATGGCAATTATTGAGAGGGAAGAGCCAGAT
TATATTGTTCCTGAAATTGAAGCAATAAATACAGATGCATTAATAGATGCTGAAAAAATGGGTTATACTGTTATTCCTAC
AGCTGAAGCTACAAAGATAACTATGAATAGGGAGTTAATAAGAAGATTGGCAGCTGAAAAATTAGGATTAAAAACTGCTA
AGTATGAATTTGCAGATTCTTTAGAAGAGTTGAGAGATGCCGTAGAAAAACTTGGCTTGCCTTGTGTAGTTAAGCCAATT
ATGTCTTCATCTGGAAAGGGGCAGAGTGTAGTTAGAAGTGAAGAGGATATAGAGAAAGCTTGGAAGATAGCTAAAGAAGG
AGCAAGAGGAATAGGAAATAGGGTTATTGTTGAAGAATTTATAAACTTTGATTATGAGATAACCTTATTAACCGCAAGAA
CTGCTGAAGGAACTAAGTTTTGTGAGCCAATAGGTCATGTCCAAATAGATGGAGATTATCATGAAAGCTGGCAACCTCAT
AATATGTCTGCTGAATTAAAAGAACAAGCTCAAGATATAGCTAAGAAGGTTACCGATGCTTTAGGTGGTTATGGAATCTT
TGGTGTTGAGTTGTTTGTTAAAGGGGATGAGGTTATATTTAGTGAAGTTTCACCAAGACCTCATGATACAGGAATGGTTA
CAATGATAACTCAAGAAATGAGTGAGTTTGAAATTCATGTTAGGGCTATTTTAGGTTTGCCAGTATCAACAAAACTTATT
CACCCAGGGGCAAGCCATGTAATAAAGGCAGAGATAAATAAATATGCTCCAAAGTATCATATAGAGGATGCTTTAAAAGT
TCCAAATACTAAGTTGAGATTGTTTGGAAAGCCAAATGCAAAGGTTGGTAGAAGAATGGGAGTTGCTTTAGCTTATGCCG
ATTCTGTAGAGAAGGCAAGGGAATTGGCTGAAAAATGTGCTCATGCAGTTAGAATTGAATGA

Upstream 100 bases:

>100_bases
TAAACAATATTTATCTCAAATGAGATAATTAACAAAAAACTATATTAATGATTCCTTTAAAAGCTAAAGTATAGAATAAA
ATTTTAATGCTAAAAATTTT

Downstream 100 bases:

>100_bases
TTGGATATTTAGATAATATTTGTCTTGTTGAAAAAATTTAAATCTATGTTTAATTAGCTTATAAAATCTATTTCTTCATT
TGAGAATTTTTTATTTAATT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MVKFMAIGTPLLKGSIKFLLLGSGELGKEVVIEAQRLGIECIAVDRYQNAPAMQVAHKSYVIDMKDYDALMAIIEREEPD
YIVPEIEAINTDALIDAEKMGYTVIPTAEATKITMNRELIRRLAAEKLGLKTAKYEFADSLEELRDAVEKLGLPCVVKPI
MSSSGKGQSVVRSEEDIEKAWKIAKEGARGIGNRVIVEEFINFDYEITLLTARTAEGTKFCEPIGHVQIDGDYHESWQPH
NMSAELKEQAQDIAKKVTDALGGYGIFGVELFVKGDEVIFSEVSPRPHDTGMVTMITQEMSEFEIHVRAILGLPVSTKLI
HPGASHVIKAEINKYAPKYHIEDALKVPNTKLRLFGKPNAKVGRRMGVALAYADSVEKARELAEKCAHAVRIE

Sequences:

>Translated_393_residues
MVKFMAIGTPLLKGSIKFLLLGSGELGKEVVIEAQRLGIECIAVDRYQNAPAMQVAHKSYVIDMKDYDALMAIIEREEPD
YIVPEIEAINTDALIDAEKMGYTVIPTAEATKITMNRELIRRLAAEKLGLKTAKYEFADSLEELRDAVEKLGLPCVVKPI
MSSSGKGQSVVRSEEDIEKAWKIAKEGARGIGNRVIVEEFINFDYEITLLTARTAEGTKFCEPIGHVQIDGDYHESWQPH
NMSAELKEQAQDIAKKVTDALGGYGIFGVELFVKGDEVIFSEVSPRPHDTGMVTMITQEMSEFEIHVRAILGLPVSTKLI
HPGASHVIKAEINKYAPKYHIEDALKVPNTKLRLFGKPNAKVGRRMGVALAYADSVEKARELAEKCAHAVRIE
>Mature_393_residues
MVKFMAIGTPLLKGSIKFLLLGSGELGKEVVIEAQRLGIECIAVDRYQNAPAMQVAHKSYVIDMKDYDALMAIIEREEPD
YIVPEIEAINTDALIDAEKMGYTVIPTAEATKITMNRELIRRLAAEKLGLKTAKYEFADSLEELRDAVEKLGLPCVVKPI
MSSSGKGQSVVRSEEDIEKAWKIAKEGARGIGNRVIVEEFINFDYEITLLTARTAEGTKFCEPIGHVQIDGDYHESWQPH
NMSAELKEQAQDIAKKVTDALGGYGIFGVELFVKGDEVIFSEVSPRPHDTGMVTMITQEMSEFEIHVRAILGLPVSTKLI
HPGASHVIKAEINKYAPKYHIEDALKVPNTKLRLFGKPNAKVGRRMGVALAYADSVEKARELAEKCAHAVRIE

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=389, Percent_Identity=57.5835475578406, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1786733, Length=253, Percent_Identity=26.8774703557312, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324702, Length=376, Percent_Identity=25.2659574468085, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43554; Mature: 43554

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKFMAIGTPLLKGSIKFLLLGSGELGKEVVIEAQRLGIECIAVDRYQNAPAMQVAHKSY
CEEEEECCCHHHCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHEEHHHE
VIDMKDYDALMAIIEREEPDYIVPEIEAINTDALIDAEKMGYTVIPTAEATKITMNRELI
EEECHHHHHHHHHHHCCCCCEECCCCEECCCCCEEEHHHCCEEEECCCCCEEEEECHHHH
RRLAAEKLGLKTAKYEFADSLEELRDAVEKLGLPCVVKPIMSSSGKGQSVVRSEEDIEKA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCHHHCCHHHHHHH
WKIAKEGARGIGNRVIVEEFINFDYEITLLTARTAEGTKFCEPIGHVQIDGDYHESWQPH
HHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCCCEEECCCCCCCCCCC
NMSAELKEQAQDIAKKVTDALGGYGIFGVELFVKGDEVIFSEVSPRPHDTGMVTMITQEM
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEHHHH
SEFEIHVRAILGLPVSTKLIHPGASHVIKAEINKYAPKYHIEDALKVPNTKLRLFGKPNA
HHHHEEEEEEECCCCCEEEECCCCCCEEEEHHHHCCCCEECCHHHCCCCCEEEEEECCCH
KVGRRMGVALAYADSVEKARELAEKCAHAVRIE
HHHHHHCEEEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVKFMAIGTPLLKGSIKFLLLGSGELGKEVVIEAQRLGIECIAVDRYQNAPAMQVAHKSY
CEEEEECCCHHHCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHEEHHHE
VIDMKDYDALMAIIEREEPDYIVPEIEAINTDALIDAEKMGYTVIPTAEATKITMNRELI
EEECHHHHHHHHHHHCCCCCEECCCCEECCCCCEEEHHHCCEEEECCCCCEEEEECHHHH
RRLAAEKLGLKTAKYEFADSLEELRDAVEKLGLPCVVKPIMSSSGKGQSVVRSEEDIEKA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCHHHCCHHHHHHH
WKIAKEGARGIGNRVIVEEFINFDYEITLLTARTAEGTKFCEPIGHVQIDGDYHESWQPH
HHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCCCEEECCCCCCCCCCC
NMSAELKEQAQDIAKKVTDALGGYGIFGVELFVKGDEVIFSEVSPRPHDTGMVTMITQEM
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEHHHH
SEFEIHVRAILGLPVSTKLIHPGASHVIKAEINKYAPKYHIEDALKVPNTKLRLFGKPNA
HHHHEEEEEEECCCCCEEEECCCCCCEEEEHHHHCCCCEECCHHHCCCCCEEEEEECCCH
KVGRRMGVALAYADSVEKARELAEKCAHAVRIE
HHHHHHCEEEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]