Click here to switch to the map view.

The map label for this gene is mjaIIM

Identifier: 15669639

GI number: 15669639

Start: 1416977

End: 1418569

Strand: Direct

Name: mjaIIM

Synonym: MJ1448

Alternate gene names: 15669639

Gene position: 1416977-1418569 (Clockwise)

Preceding gene: 15669638

Following gene: 15669640

Centisome position: 85.11

GC content: 27.87

Gene sequence:

>1593_bases
ATGAATCTGGATGCATGGTTAGATATACAGCCAGCAAAAAAACTTTACACAATAAAAGAGGCATCAAGAATATTAACAAA
AAAGTTTGGTAAGGAAATTAAAGAGCATAATATTTCATATTTAGTGCAGTATGGAAGAGTCAATAAGTATAAAATCAAAA
ATAGGGTTTATGTAGATATTGATGAGGTTGAAAACTATTATAAAAAATTATTTTTTGAAAAAAGAAAAGAATGGGAAGAA
AAATTGGGATTTAAATTGGATTGGGATTTAGCATTTGATTTGTTATCTGAGAAGGAGAGAACTAAACATGTTCATGGAAT
TCACCCATACAAAGGAAAGTTTATTCCACAATTGGTAGAGTATTTTTTAAAGAGGCATTTTAACGTTGGAGATATTATAA
TAGACCCGTTTATGGGTTCTGGAACAACATTAGTGCAATGTATGGAGATGGGCATTAATTCTATTGGCATTGATATATCA
CCATTTAACTGTTTAATTGCTGAAGTTAAATTGCAGAAATATGATATTCAAAAATTAAAGAAAATTTTATTAGATATGCT
AAATAAAACAAAGGAATTTTCAAAAAATTTAGGAGATGATGAATTTGTTAAAGAAATGGATAAATTAATTGAGAAATACA
ACAAAAAATACTTTACCTTAGAATATAAAAGAAAATTATCAAAAAAAGAAATTGATGAAGATAGCTATTCCGAAAAAATC
ATGGAAATGTTTTATTTAGAGTATAAAAAACTTAAGGAAAAATACTGCAAAAATGATGATGAGTTTGATGACATTTTTAA
AGATAAGCCATTTTTATATAAGTGGTATTCTCCAAGAATTAGGGCAGAATTAAACTTTTATTTAAATTTAATTAAAGACT
GCAGAGATGAAACAATAAAAAAAGTGGCAATGATAATTTTGAGTAGAACGGCAAGGTCTGTTAGAGGAACTACTCATTTT
GATTTAGCCACACTAAAAGAGCCAGTTTTTGACCCATACTATTGCTATAAGCATAAAAAAATTTGCAGACCAGTTCAAAC
AATTTTAAGACACTTAGAAGAATATACAAATGATGTAATTAGTAGAATAGAAGAATTTTCAAAGATTAGGAAAGATGCAT
ATTATTTAATCATTAATGGAGATTCAAGGACTGTTGATATTGAAGAAGAACTAAAAAAACACCCAAACTTTTATGAACTT
TATAAAAACAAAAAAATTGATGGTATTTTTACATCTCCTCCTTATTTAGGGCAGATTGATTATCATGAGCAGCATGCCTA
TGCTTATGAGCTTTTTGACATTCCAAGATTGGATGAGTTGGAGATAGGACCTAAATTTAAAGGTAGTTCAAAAAAAGCAC
AAAAAGAGTATATTGAAGGCATATCCGATGTTTTAATCAACATGAAAAGATTTTTAAATGAAGACGCAAAGATTTTCATT
GTAGTTAATGATAAAAAGAACCTATATAAAGAGATTTTTGAGAAAAGTGGGCTTATTTTAGTTAGAGAGTTTAAAAGACC
GGTCTTAAATAGAACAGAAAGAGATAGAAACCCATACTATGAAAGCATCTTTGAATTAAAAATGGAGGAATAG

Upstream 100 bases:

>100_bases
ACCCAGGAAGAGTAATTAGGATGTTTATAAACAAGATGGGTTATGATATAGACACTTATAGGCTTTATTTTGATGAGGAT
GAGGATATAGGGGAGGAATT

Downstream 100 bases:

>100_bases
CTATGCCACTAAGTAAAAATGTTATAGAAAAAATTAGCATTGAGACAATAAGAGTATTAAAATCAAGATTTGATACAATA
TCAGATGAAGACATAAAAAT

Product: type II R/M system modification methyltransferase

Products: NA

Alternate protein names: M.MjaII; N-4 cytosine-specific methyltransferase MjaII

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MNLDAWLDIQPAKKLYTIKEASRILTKKFGKEIKEHNISYLVQYGRVNKYKIKNRVYVDIDEVENYYKKLFFEKRKEWEE
KLGFKLDWDLAFDLLSEKERTKHVHGIHPYKGKFIPQLVEYFLKRHFNVGDIIIDPFMGSGTTLVQCMEMGINSIGIDIS
PFNCLIAEVKLQKYDIQKLKKILLDMLNKTKEFSKNLGDDEFVKEMDKLIEKYNKKYFTLEYKRKLSKKEIDEDSYSEKI
MEMFYLEYKKLKEKYCKNDDEFDDIFKDKPFLYKWYSPRIRAELNFYLNLIKDCRDETIKKVAMIILSRTARSVRGTTHF
DLATLKEPVFDPYYCYKHKKICRPVQTILRHLEEYTNDVISRIEEFSKIRKDAYYLIINGDSRTVDIEEELKKHPNFYEL
YKNKKIDGIFTSPPYLGQIDYHEQHAYAYELFDIPRLDELEIGPKFKGSSKKAQKEYIEGISDVLINMKRFLNEDAKIFI
VVNDKKNLYKEIFEKSGLILVREFKRPVLNRTERDRNPYYESIFELKMEE

Sequences:

>Translated_530_residues
MNLDAWLDIQPAKKLYTIKEASRILTKKFGKEIKEHNISYLVQYGRVNKYKIKNRVYVDIDEVENYYKKLFFEKRKEWEE
KLGFKLDWDLAFDLLSEKERTKHVHGIHPYKGKFIPQLVEYFLKRHFNVGDIIIDPFMGSGTTLVQCMEMGINSIGIDIS
PFNCLIAEVKLQKYDIQKLKKILLDMLNKTKEFSKNLGDDEFVKEMDKLIEKYNKKYFTLEYKRKLSKKEIDEDSYSEKI
MEMFYLEYKKLKEKYCKNDDEFDDIFKDKPFLYKWYSPRIRAELNFYLNLIKDCRDETIKKVAMIILSRTARSVRGTTHF
DLATLKEPVFDPYYCYKHKKICRPVQTILRHLEEYTNDVISRIEEFSKIRKDAYYLIINGDSRTVDIEEELKKHPNFYEL
YKNKKIDGIFTSPPYLGQIDYHEQHAYAYELFDIPRLDELEIGPKFKGSSKKAQKEYIEGISDVLINMKRFLNEDAKIFI
VVNDKKNLYKEIFEKSGLILVREFKRPVLNRTERDRNPYYESIFELKMEE
>Mature_530_residues
MNLDAWLDIQPAKKLYTIKEASRILTKKFGKEIKEHNISYLVQYGRVNKYKIKNRVYVDIDEVENYYKKLFFEKRKEWEE
KLGFKLDWDLAFDLLSEKERTKHVHGIHPYKGKFIPQLVEYFLKRHFNVGDIIIDPFMGSGTTLVQCMEMGINSIGIDIS
PFNCLIAEVKLQKYDIQKLKKILLDMLNKTKEFSKNLGDDEFVKEMDKLIEKYNKKYFTLEYKRKLSKKEIDEDSYSEKI
MEMFYLEYKKLKEKYCKNDDEFDDIFKDKPFLYKWYSPRIRAELNFYLNLIKDCRDETIKKVAMIILSRTARSVRGTTHF
DLATLKEPVFDPYYCYKHKKICRPVQTILRHLEEYTNDVISRIEEFSKIRKDAYYLIINGDSRTVDIEEELKKHPNFYEL
YKNKKIDGIFTSPPYLGQIDYHEQHAYAYELFDIPRLDELEIGPKFKGSSKKAQKEYIEGISDVLINMKRFLNEDAKIFI
VVNDKKNLYKEIFEKSGLILVREFKRPVLNRTERDRNPYYESIFELKMEE

Specific function: This methylase recognizes the double-stranded sequence GGNC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the MjaII endonuclease (Potential)

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTM2_METJA (Q58843)

Other databases:

- EMBL:   L77117
- PIR:   G64480
- RefSeq:   NP_248452.1
- ProteinModelPortal:   Q58843
- SMR:   Q58843
- REBASE:   3896
- GeneID:   1452352
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1448
- NMPDR:   fig|243232.1.peg.1489
- TIGR:   MJ1448
- HOGENOM:   HBG615333
- OMA:   IDYHEQH
- ProtClustDB:   CLSK796871
- BioCyc:   MJAN243232:MJ_1448-MONOMER
- BRENDA:   2.1.1.113
- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091
- PRINTS:   PR00508

Pfam domain/function: PF01555 N6_N4_Mtase

EC number: =2.1.1.113

Molecular weight: Translated: 63756; Mature: 63756

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDAWLDIQPAKKLYTIKEASRILTKKFGKEIKEHNISYLVQYGRVNKYKIKNRVYVDI
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCEEEEEH
DEVENYYKKLFFEKRKEWEEKLGFKLDWDLAFDLLSEKERTKHVHGIHPYKGKFIPQLVE
HHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
YFLKRHFNVGDIIIDPFMGSGTTLVQCMEMGINSIGIDISPFNCLIAEVKLQKYDIQKLK
HHHHHCCCCCCEEECCCCCCCHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
KILLDMLNKTKEFSKNLGDDEFVKEMDKLIEKYNKKYFTLEYKRKLSKKEIDEDSYSEKI
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCHHHHHHH
MEMFYLEYKKLKEKYCKNDDEFDDIFKDKPFLYKWYSPRIRAELNFYLNLIKDCRDETIK
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
KVAMIILSRTARSVRGTTHFDLATLKEPVFDPYYCYKHKKICRPVQTILRHLEEYTNDVI
HHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRIEEFSKIRKDAYYLIINGDSRTVDIEEELKKHPNFYELYKNKKIDGIFTSPPYLGQID
HHHHHHHHHCCCEEEEEEECCCCEEEHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCC
YHEQHAYAYELFDIPRLDELEIGPKFKGSSKKAQKEYIEGISDVLINMKRFLNEDAKIFI
CCHHHHHEEHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VVNDKKNLYKEIFEKSGLILVREFKRPVLNRTERDRNPYYESIFELKMEE
EECCCHHHHHHHHHCCCEEEEHHHHHHHHCCHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNLDAWLDIQPAKKLYTIKEASRILTKKFGKEIKEHNISYLVQYGRVNKYKIKNRVYVDI
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCEEEEEH
DEVENYYKKLFFEKRKEWEEKLGFKLDWDLAFDLLSEKERTKHVHGIHPYKGKFIPQLVE
HHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
YFLKRHFNVGDIIIDPFMGSGTTLVQCMEMGINSIGIDISPFNCLIAEVKLQKYDIQKLK
HHHHHCCCCCCEEECCCCCCCHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
KILLDMLNKTKEFSKNLGDDEFVKEMDKLIEKYNKKYFTLEYKRKLSKKEIDEDSYSEKI
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCHHHHHHH
MEMFYLEYKKLKEKYCKNDDEFDDIFKDKPFLYKWYSPRIRAELNFYLNLIKDCRDETIK
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
KVAMIILSRTARSVRGTTHFDLATLKEPVFDPYYCYKHKKICRPVQTILRHLEEYTNDVI
HHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRIEEFSKIRKDAYYLIINGDSRTVDIEEELKKHPNFYELYKNKKIDGIFTSPPYLGQID
HHHHHHHHHCCCEEEEEEECCCCEEEHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCC
YHEQHAYAYELFDIPRLDELEIGPKFKGSSKKAQKEYIEGISDVLINMKRFLNEDAKIFI
CCHHHHHEEHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VVNDKKNLYKEIFEKSGLILVREFKRPVLNRTERDRNPYYESIFELKMEE
EECCCHHHHHHHHHCCCEEEEHHHHHHHHCCHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087