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The map label for this gene is cobS

Identifier: 15669629

GI number: 15669629

Start: 1405750

End: 1406526

Strand: Direct

Name: cobS

Synonym: MJ1438

Alternate gene names: 15669629

Gene position: 1405750-1406526 (Clockwise)

Preceding gene: 15669628

Following gene: 15669630

Centisome position: 84.43

GC content: 28.57

Gene sequence:

>777_bases
TTGGGGATAACAATGTTTAAGGAATTTAAAGCACTGTTGTCATTTTTTACAAGGATTCCAATCTATGTAGAAGATTTTGA
TTTTGAAAACATTGCCAACTATTTTTATCTGATTATTTTAATTGGATATGTGTTTGGAATTTTTAGTTTGATATTAGGTT
ATATTTTTAGTTTTTTGCTCCCCAATTTTTTATCTGCTGTTTTAATTTTGTTTTTTATTGAATATTTAAACGGATTCCAC
CATATAGATGGTTTAATTGACTTTGGAGATGGGTGGATGGCTGTTGGAGATAAGAGAAAGAAATTAATGGCTATGAAAGA
TAGATATATTGGCTGTGGAGGAGTAGTTTTTGCAATATTTTTTAATTTAATGGCAGTCATATCATTATCTTATATTTTAG
ACATTAATATCCTATATCTATTGGTTGGAGAGGTTTGTGCAAAGCTTGGAATGCTAAGCTGTTCAACCTTTGGAAATCCT
TTAATTGAAGGAACTGGGAGATACTTTGTTAAAAAGGCAGATGAAAAATTTTTAACAATTGGTATTATTTTATCTCTCCC
ATTACTTTTAATATTCAGTGGGATTGAAAGGAAGATAGTTATTATTGCCATAATAACGACAATAATTACTGGCTTATGTA
TGGCTAAAATAGCCAAAAGACATTTTGGAGGAGTTAATGGAGATGTTTTAGGAGCTTCAAATGAAATAACAAGAGTTGTT
GTTTTATTATCAATTATAGCAAGTATTAAAGTATTTAGTATATATTTATTAGGTTAA

Upstream 100 bases:

>100_bases
GACATGGAAGATGATGAATATAGCGATTACACAATAAACTCCCTTCAAGAGCTTGTAGATATTGTTAAAAATTTAAAAAA
GGATTAAAATATAAAATTTT

Downstream 100 bases:

>100_bases
AATGATTAGAGGGAGGATTATGAGAAAATTCCTAATACTATCTATGTTAATCTTCACAACTCTATGTGGCTGTGTTGATT
TCTCATCCTCTAATTCTAAC

Product: cobalamin (5'-phosphate) synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MGITMFKEFKALLSFFTRIPIYVEDFDFENIANYFYLIILIGYVFGIFSLILGYIFSFLLPNFLSAVLILFFIEYLNGFH
HIDGLIDFGDGWMAVGDKRKKLMAMKDRYIGCGGVVFAIFFNLMAVISLSYILDINILYLLVGEVCAKLGMLSCSTFGNP
LIEGTGRYFVKKADEKFLTIGIILSLPLLLIFSGIERKIVIIAIITTIITGLCMAKIAKRHFGGVNGDVLGASNEITRVV
VLLSIIASIKVFSIYLLG

Sequences:

>Translated_258_residues
MGITMFKEFKALLSFFTRIPIYVEDFDFENIANYFYLIILIGYVFGIFSLILGYIFSFLLPNFLSAVLILFFIEYLNGFH
HIDGLIDFGDGWMAVGDKRKKLMAMKDRYIGCGGVVFAIFFNLMAVISLSYILDINILYLLVGEVCAKLGMLSCSTFGNP
LIEGTGRYFVKKADEKFLTIGIILSLPLLLIFSGIERKIVIIAIITTIITGLCMAKIAKRHFGGVNGDVLGASNEITRVV
VLLSIIASIKVFSIYLLG
>Mature_257_residues
GITMFKEFKALLSFFTRIPIYVEDFDFENIANYFYLIILIGYVFGIFSLILGYIFSFLLPNFLSAVLILFFIEYLNGFHH
IDGLIDFGDGWMAVGDKRKKLMAMKDRYIGCGGVVFAIFFNLMAVISLSYILDINILYLLVGEVCAKLGMLSCSTFGNPL
IEGTGRYFVKKADEKFLTIGIILSLPLLLIFSGIERKIVIIAIITTIITGLCMAKIAKRHFGGVNGDVLGASNEITRVVV
LLSIIASIKVFSIYLLG

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=257, Percent_Identity=26.4591439688716, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_METJA (Q58833)

Other databases:

- EMBL:   L77117
- PIR:   E64479
- RefSeq:   NP_248442.1
- ProteinModelPortal:   Q58833
- GeneID:   1452342
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1438
- NMPDR:   fig|243232.1.peg.1479
- TIGR:   MJ1438
- HOGENOM:   HBG729392
- OMA:   CSILAVF
- ProtClustDB:   CLSK2749994
- BioCyc:   MJAN243232:MJ_1438-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 28778; Mature: 28647

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xee5e9d4)-; HASH(0xeb5add4)-; HASH(0xe95a6c4)-; HASH(0xe4934f8)-; HASH(0xe5ac3d8)-; HASH(0xdbff06c)-; HASH(0xeb5f3cc)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGITMFKEFKALLSFFTRIPIYVEDFDFENIANYFYLIILIGYVFGIFSLILGYIFSFLL
CCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PNFLSAVLILFFIEYLNGFHHIDGLIDFGDGWMAVGDKRKKLMAMKDRYIGCGGVVFAIF
HHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEECCHHHHHHHHHHHHCCCCHHHHHHHH
FNLMAVISLSYILDINILYLLVGEVCAKLGMLSCSTFGNPLIEGTGRYFVKKADEKFLTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHCCCHHHHH
GIILSLPLLLIFSGIERKIVIIAIITTIITGLCMAKIAKRHFGGVNGDVLGASNEITRVV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
VLLSIIASIKVFSIYLLG
HHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
GITMFKEFKALLSFFTRIPIYVEDFDFENIANYFYLIILIGYVFGIFSLILGYIFSFLL
CCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PNFLSAVLILFFIEYLNGFHHIDGLIDFGDGWMAVGDKRKKLMAMKDRYIGCGGVVFAIF
HHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEECCHHHHHHHHHHHHCCCCHHHHHHHH
FNLMAVISLSYILDINILYLLVGEVCAKLGMLSCSTFGNPLIEGTGRYFVKKADEKFLTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHCCCHHHHH
GIILSLPLLLIFSGIERKIVIIAIITTIITGLCMAKIAKRHFGGVNGDVLGASNEITRVV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
VLLSIIASIKVFSIYLLG
HHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087