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The map label for this gene is pyrI

Identifier: 15669596

GI number: 15669596

Start: 1367727

End: 1368176

Strand: Direct

Name: pyrI

Synonym: MJ1406

Alternate gene names: 15669596

Gene position: 1367727-1368176 (Clockwise)

Preceding gene: 15669595

Following gene: 15669597

Centisome position: 82.15

GC content: 27.78

Gene sequence:

>450_bases
GTGATTCCTATGGAGGAGTTAAAAGTTAAAAAAATTACAAATGGGACTGTAATTGACCATATAGATGCTGGAAAGGCATT
AATGGTTTTTAAGGTTTTAAATGTCCCAAAAGAGACATCTGTGATGATAGCCATTAATGTCCCATCAAAAAAGAAAGGAA
AGAAAGATATTTTAAAAATTGAAGGAATTGAATTAAAAAAGGAGGATGTTGATAAAATATCTTTAATTTCTCCAGATGTA
ACTATCAACATCATTAGAAACGGAAAAGTTGTTGAAAAACTTAAACCACAAATTCCAGATGAGATAGAAGGGACATTAAA
ATGCACAAATCCAAATTGCATAACAAATAAAGAAAAAGTTAGAGGAAAATTTAAAATAGAAAGTAAAAACCCATTAAAAA
TTAGATGCTATTATTGTGAGAAGTTTTTAAATGAAGTAATTTTTGAATGA

Upstream 100 bases:

>100_bases
AATGTAAATAAAAAATTAGAGGTATTGAATGAGATAAAAGAAACACAAATAAAAATGCTAAAAATATTAGAGAAAATTGA
ACAAAAGCTTTAAATTTTAA

Downstream 100 bases:

>100_bases
ATAATTTAAAAGTGGTATTATGGAATTAAAAGGAAGAAGTATTTCAAAGCATAGAGGCAAAGCCTCTATTGCTATACTCC
AGAGCGGGGTTGCCAGAGGC

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MIPMEELKVKKITNGTVIDHIDAGKALMVFKVLNVPKETSVMIAINVPSKKKGKKDILKIEGIELKKEDVDKISLISPDV
TINIIRNGKVVEKLKPQIPDEIEGTLKCTNPNCITNKEKVRGKFKIESKNPLKIRCYYCEKFLNEVIFE

Sequences:

>Translated_149_residues
MIPMEELKVKKITNGTVIDHIDAGKALMVFKVLNVPKETSVMIAINVPSKKKGKKDILKIEGIELKKEDVDKISLISPDV
TINIIRNGKVVEKLKPQIPDEIEGTLKCTNPNCITNKEKVRGKFKIESKNPLKIRCYYCEKFLNEVIFE
>Mature_149_residues
MIPMEELKVKKITNGTVIDHIDAGKALMVFKVLNVPKETSVMIAINVPSKKKGKKDILKIEGIELKKEDVDKISLISPDV
TINIIRNGKVVEKLKPQIPDEIEGTLKCTNPNCITNKEKVRGKFKIESKNPLKIRCYYCEKFLNEVIFE

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=153, Percent_Identity=37.9084967320261, Blast_Score=104, Evalue=2e-24,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_METJA (Q58801)

Other databases:

- EMBL:   L77117
- PIR:   E64475
- RefSeq:   NP_248409.1
- PDB:   2YWW
- PDBsum:   2YWW
- ProteinModelPortal:   Q58801
- SMR:   Q58801
- GeneID:   1452309
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1406
- NMPDR:   fig|243232.1.peg.1446
- TIGR:   MJ1406
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- ProtClustDB:   PRK00893
- BioCyc:   MJAN243232:MJ_1406-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 16907; Mature: 16907

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPMEELKVKKITNGTVIDHIDAGKALMVFKVLNVPKETSVMIAINVPSKKKGKKDILKI
CCCHHHCEEEEECCCEEEEECCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCEEEE
EGIELKKEDVDKISLISPDVTINIIRNGKVVEKLKPQIPDEIEGTLKCTNPNCITNKEKV
CCCEECCCCCCEEEEECCCEEEEEEECCEEHHHHCCCCCCCCCCEEEECCCCCCCCHHHC
RGKFKIESKNPLKIRCYYCEKFLNEVIFE
CEEEEECCCCCEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MIPMEELKVKKITNGTVIDHIDAGKALMVFKVLNVPKETSVMIAINVPSKKKGKKDILKI
CCCHHHCEEEEECCCEEEEECCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCEEEE
EGIELKKEDVDKISLISPDVTINIIRNGKVVEKLKPQIPDEIEGTLKCTNPNCITNKEKV
CCCEECCCCCCEEEEECCCEEEEEEECCEEHHHHCCCCCCCCCCEEEECCCCCCCCHHHC
RGKFKIESKNPLKIRCYYCEKFLNEVIFE
CEEEEECCCCCEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10748118