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The map label for this gene is carB2

Identifier: 15669571

GI number: 15669571

Start: 1329175

End: 1331031

Strand: Direct

Name: carB2

Synonym: MJ1381

Alternate gene names: 15669571

Gene position: 1329175-1331031 (Clockwise)

Preceding gene: 15669570

Following gene: 15669576

Centisome position: 79.83

GC content: 36.13

Gene sequence:

>1857_bases
ATGGATATGGAAAAATTAAAGGAAATATTATTGAAAGCAAAAAAATTGGGATTCTCTGATAAACAGATAGCTAATTTATT
AGGAATGGATGAGATAGAAGTTAGGGACTTGAGAAAGAAGCTCAATATCATTCCTTTATACAAAATGGTAGATACCTGTG
CTGCTGAGTTTGAGGCAAAAACCCCATACTATTACTCTGCCTATGAGACATTTGTTTATAAGGAGCAAGATGAAAGCAAT
CCATCAGATAGAAAAAAAGTTATAATCATCGGTTCTGGGCCGATAAGGATTGGTCAGGGGATAGAATTTGATTATTCAAG
TGTTCATGCAGTTTTAGCTTTAAAGGAAATGGGAATTGAAGCTATAATCATAAATAACAACCCAGAGACAGTTTCAACCG
ACTATGATACATCAGACAAGCTTTATTTTGAACCAATAACCTTTGAAGAGGTATTAAATATTGCTGAAAGAGAAAAGGAG
AAAGGAGAGCTTTTGGGAGTTATAGTTCAATTTGGTGGGCAAACAGCAATAAACTTAGCCATGAAATTAAAAAATGCGGG
AGTTAATATCTTAGGAACCACTCCAGAGAATATAAATGCTGCTGAGGATAGAGAAGAGTTTTCAAAACTTTTAAAGAAGT
TAAATATTCCTCAAGCTGAAGGAGGAACAGCATATACAAAAGAAGAGGCATTGGAGATAGCTAAAAGAATTGGTTATCCT
GTTTTAGTTAGGCCTTCCTATGTTTTAGGAGGAAGGGCAATGCAAATTGTTTATAGCGAGGATGAGTTAATTGAGTATAT
GGAAGAGGCAGTTAAAGTTTCAGAGGAGCATCCAGTGTTGATTGATAAATTTTTAGAAGATGCTATTGAGTTGGATGTTG
ATGCTGTTTGTGATGGAGAGAGTGTTTTAATTGGGGCAATAATGGAGCATATTGAAGAGGCTGGAGTGCATAGTGGAGAT
TCAGCAACAGTTATTCCTCCTCAAACTTTACCAAAGGAGATAATTGATACTGTTATAGATTACACTGCCAAATTAGCAAG
GGCTTTAAACATAGTGGGGCTTTTAAACGTGCAGTATGCAGTTAAAGATGGAGTTGTTTATGTTTTGGAAGCAAACCCAA
GGGCTTCAAGAACTGTTCCTTATGTGAGTAAATCAGTTGGAATTCCATTGGCTAAGTTAGCCACAAAGATTATGCTCGGC
AAAAAGTTGGAGGAGTTAATTAAAGATTATGATGTTGAAAAAGTTGCTGAGAAGGTTTGGATTGCAAAGCCGAAGTATGT
ATCAATTAAAGAGGCAGTATTCCCATTCCAAAAATTGCCTGGAGTAGACCCTGTTTTAGGGCCTGAGATGAAATCTACTG
GAGAGGCTATTGGAATAGATAAAGACTTTGGTAGGGCATACTATAAAGCTCAGTTATCTGCAAATATGGAATTGCCAATC
GTTGGAAATGTCTTTATAAGTGTTAGAGATAGGGATAAGAAGCATATCGTAGATGTTGCTAAAAAGTTGCATGAGCTTGG
CTTTACAATATACGCAACAGAAGGAACTGCTAAAGTGTTGAGAGAAAATGGAATTCCAGCAATACTTGTTAAGAAGATAT
CTGAAAGTCCAAATGATAACATCCTAAAGTTAATGAGAGATGGTAAGATGCACTTAATAATAAACACTTCCTCTGGAAAG
AAGGCAAAGTCAGACGGGTATTATATAAGAAGGGCAGCAGTGGATTTGGGTATTCCTTATATAACTACAATCCCTGGAGC
AAAAGCTGCTGTTAAGGCAATAGAAGCTGTAAAAACTGGAGAGTTAAGTGTTTATTCATTAGATGAGCTTGATGCAAGGA
TAAAAAATAGGTTTTAA

Upstream 100 bases:

>100_bases
ATATCCTTCTTTGGAAGATAGTTAAAGAAGACGTTCCTAAAATAAAGAAAGAAGTTGAGATAGTTTTAAAAGATATTGAA
AATAAAAAATAGGTGATTAT

Downstream 100 bases:

>100_bases
GCTTTAGCTTCAATAATCAACTTATAATCCTCTTCTGGAATTTCTCTCATTGCTTTTCCCATCAAATGCCCACTCCACTT
CTTTTTGTTTGTTATGAATT

Product: carbamoyl-phosphate synthase subunit CarB2

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MDMEKLKEILLKAKKLGFSDKQIANLLGMDEIEVRDLRKKLNIIPLYKMVDTCAAEFEAKTPYYYSAYETFVYKEQDESN
PSDRKKVIIIGSGPIRIGQGIEFDYSSVHAVLALKEMGIEAIIINNNPETVSTDYDTSDKLYFEPITFEEVLNIAEREKE
KGELLGVIVQFGGQTAINLAMKLKNAGVNILGTTPENINAAEDREEFSKLLKKLNIPQAEGGTAYTKEEALEIAKRIGYP
VLVRPSYVLGGRAMQIVYSEDELIEYMEEAVKVSEEHPVLIDKFLEDAIELDVDAVCDGESVLIGAIMEHIEEAGVHSGD
SATVIPPQTLPKEIIDTVIDYTAKLARALNIVGLLNVQYAVKDGVVYVLEANPRASRTVPYVSKSVGIPLAKLATKIMLG
KKLEELIKDYDVEKVAEKVWIAKPKYVSIKEAVFPFQKLPGVDPVLGPEMKSTGEAIGIDKDFGRAYYKAQLSANMELPI
VGNVFISVRDRDKKHIVDVAKKLHELGFTIYATEGTAKVLRENGIPAILVKKISESPNDNILKLMRDGKMHLIINTSSGK
KAKSDGYYIRRAAVDLGIPYITTIPGAKAAVKAIEAVKTGELSVYSLDELDARIKNRF

Sequences:

>Translated_618_residues
MDMEKLKEILLKAKKLGFSDKQIANLLGMDEIEVRDLRKKLNIIPLYKMVDTCAAEFEAKTPYYYSAYETFVYKEQDESN
PSDRKKVIIIGSGPIRIGQGIEFDYSSVHAVLALKEMGIEAIIINNNPETVSTDYDTSDKLYFEPITFEEVLNIAEREKE
KGELLGVIVQFGGQTAINLAMKLKNAGVNILGTTPENINAAEDREEFSKLLKKLNIPQAEGGTAYTKEEALEIAKRIGYP
VLVRPSYVLGGRAMQIVYSEDELIEYMEEAVKVSEEHPVLIDKFLEDAIELDVDAVCDGESVLIGAIMEHIEEAGVHSGD
SATVIPPQTLPKEIIDTVIDYTAKLARALNIVGLLNVQYAVKDGVVYVLEANPRASRTVPYVSKSVGIPLAKLATKIMLG
KKLEELIKDYDVEKVAEKVWIAKPKYVSIKEAVFPFQKLPGVDPVLGPEMKSTGEAIGIDKDFGRAYYKAQLSANMELPI
VGNVFISVRDRDKKHIVDVAKKLHELGFTIYATEGTAKVLRENGIPAILVKKISESPNDNILKLMRDGKMHLIINTSSGK
KAKSDGYYIRRAAVDLGIPYITTIPGAKAAVKAIEAVKTGELSVYSLDELDARIKNRF
>Mature_618_residues
MDMEKLKEILLKAKKLGFSDKQIANLLGMDEIEVRDLRKKLNIIPLYKMVDTCAAEFEAKTPYYYSAYETFVYKEQDESN
PSDRKKVIIIGSGPIRIGQGIEFDYSSVHAVLALKEMGIEAIIINNNPETVSTDYDTSDKLYFEPITFEEVLNIAEREKE
KGELLGVIVQFGGQTAINLAMKLKNAGVNILGTTPENINAAEDREEFSKLLKKLNIPQAEGGTAYTKEEALEIAKRIGYP
VLVRPSYVLGGRAMQIVYSEDELIEYMEEAVKVSEEHPVLIDKFLEDAIELDVDAVCDGESVLIGAIMEHIEEAGVHSGD
SATVIPPQTLPKEIIDTVIDYTAKLARALNIVGLLNVQYAVKDGVVYVLEANPRASRTVPYVSKSVGIPLAKLATKIMLG
KKLEELIKDYDVEKVAEKVWIAKPKYVSIKEAVFPFQKLPGVDPVLGPEMKSTGEAIGIDKDFGRAYYKAQLSANMELPI
VGNVFISVRDRDKKHIVDVAKKLHELGFTIYATEGTAKVLRENGIPAILVKKISESPNDNILKLMRDGKMHLIINTSSGK
KAKSDGYYIRRAAVDLGIPYITTIPGAKAAVKAIEAVKTGELSVYSLDELDARIKNRF

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI170295797, Length=595, Percent_Identity=41.1764705882353, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI21361331, Length=595, Percent_Identity=41.1764705882353, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI169790915, Length=595, Percent_Identity=41.1764705882353, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI18105007, Length=602, Percent_Identity=37.5415282392027, Blast_Score=388, Evalue=1e-108,
Organism=Escherichia coli, GI1786216, Length=609, Percent_Identity=51.559934318555, Blast_Score=596, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI193204318, Length=608, Percent_Identity=38.9802631578947, Blast_Score=389, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6322331, Length=622, Percent_Identity=39.871382636656, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322569, Length=605, Percent_Identity=38.1818181818182, Blast_Score=389, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24642586, Length=602, Percent_Identity=37.8737541528239, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45555749, Length=518, Percent_Identity=39.96138996139, Blast_Score=363, Evalue=1e-100,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARB2_METJA (Q58776)

Other databases:

- EMBL:   L77117
- PIR:   D64472
- RefSeq:   NP_248384.1
- ProteinModelPortal:   Q58776
- GeneID:   1452284
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1381
- NMPDR:   fig|243232.1.peg.1421
- TIGR:   MJ1381
- HOGENOM:   HBG405439
- OMA:   CVQAVFA
- ProtClustDB:   CLSK2300410
- BioCyc:   MJAN243232:MJ_1378-C-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01210_A
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:1.10.1030.10
- Gene3D:   G3DSA:3.40.50.1380
- Gene3D:   G3DSA:3.40.50.20
- PRINTS:   PR00098
- SMART:   SM00851

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; PF02142 MGS; SSF48108 CarbamoylP_synth_lsu_oligo; SSF52335 MGS-like_dom; SSF52440 PreATP-grasp-like

EC number: =6.3.5.5

Molecular weight: Translated: 68454; Mature: 68454

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50975 ATP_GRASP; PS00866 CPSASE_1; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMEKLKEILLKAKKLGFSDKQIANLLGMDEIEVRDLRKKLNIIPLYKMVDTCAAEFEAK
CCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
TPYYYSAYETFVYKEQDESNPSDRKKVIIIGSGPIRIGQGIEFDYSSVHAVLALKEMGIE
CCEEEEEEEEEEEECCCCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCEE
AIIINNNPETVSTDYDTSDKLYFEPITFEEVLNIAEREKEKGELLGVIVQFGGQTAINLA
EEEECCCCCEEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHEEEHHHCCCCCEEHEE
MKLKNAGVNILGTTPENINAAEDREEFSKLLKKLNIPQAEGGTAYTKEEALEIAKRIGYP
EEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
VLVRPSYVLGGRAMQIVYSEDELIEYMEEAVKVSEEHPVLIDKFLEDAIELDVDAVCDGE
EEECCCEEECCEEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCC
SVLIGAIMEHIEEAGVHSGDSATVIPPQTLPKEIIDTVIDYTAKLARALNIVGLLNVQYA
HHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
VKDGVVYVLEANPRASRTVPYVSKSVGIPLAKLATKIMLGKKLEELIKDYDVEKVAEKVW
ECCCEEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IAKPKYVSIKEAVFPFQKLPGVDPVLGPEMKSTGEAIGIDKDFGRAYYKAQLSANMELPI
CCCCCCEEHHHHHCCHHHCCCCCCCCCCCCCCCCCEECCCHHHHHHHEEEECCCCCCCEE
VGNVFISVRDRDKKHIVDVAKKLHELGFTIYATEGTAKVLRENGIPAILVKKISESPNDN
EEEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCEEEHHHHCCCCCHH
ILKLMRDGKMHLIINTSSGKKAKSDGYYIRRAAVDLGIPYITTIPGAKAAVKAIEAVKTG
HHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEHHHCCCCEEEECCCHHHHHHHHHHHHCC
ELSVYSLDELDARIKNRF
CEEEEEHHHHHHHHHCCC
>Mature Secondary Structure
MDMEKLKEILLKAKKLGFSDKQIANLLGMDEIEVRDLRKKLNIIPLYKMVDTCAAEFEAK
CCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
TPYYYSAYETFVYKEQDESNPSDRKKVIIIGSGPIRIGQGIEFDYSSVHAVLALKEMGIE
CCEEEEEEEEEEEECCCCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCEE
AIIINNNPETVSTDYDTSDKLYFEPITFEEVLNIAEREKEKGELLGVIVQFGGQTAINLA
EEEECCCCCEEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHEEEHHHCCCCCEEHEE
MKLKNAGVNILGTTPENINAAEDREEFSKLLKKLNIPQAEGGTAYTKEEALEIAKRIGYP
EEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
VLVRPSYVLGGRAMQIVYSEDELIEYMEEAVKVSEEHPVLIDKFLEDAIELDVDAVCDGE
EEECCCEEECCEEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCC
SVLIGAIMEHIEEAGVHSGDSATVIPPQTLPKEIIDTVIDYTAKLARALNIVGLLNVQYA
HHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
VKDGVVYVLEANPRASRTVPYVSKSVGIPLAKLATKIMLGKKLEELIKDYDVEKVAEKVW
ECCCEEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IAKPKYVSIKEAVFPFQKLPGVDPVLGPEMKSTGEAIGIDKDFGRAYYKAQLSANMELPI
CCCCCCEEHHHHHCCHHHCCCCCCCCCCCCCCCCCEECCCHHHHHHHEEEECCCCCCCEE
VGNVFISVRDRDKKHIVDVAKKLHELGFTIYATEGTAKVLRENGIPAILVKKISESPNDN
EEEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCEEEHHHHCCCCCHH
ILKLMRDGKMHLIINTSSGKKAKSDGYYIRRAAVDLGIPYITTIPGAKAAVKAIEAVKTG
HHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEHHHCCCCEEEECCCHHHHHHHHHHHHCC
ELSVYSLDELDARIKNRF
CEEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087