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The map label for this gene is glnA

Identifier: 15669537

GI number: 15669537

Start: 1292927

End: 1294291

Strand: Direct

Name: glnA

Synonym: MJ1346

Alternate gene names: 15669537

Gene position: 1292927-1294291 (Clockwise)

Preceding gene: 15669536

Following gene: 15669538

Centisome position: 77.65

GC content: 39.93

Gene sequence:

>1365_bases
TTGCATTGGGGGATGGAAATGAACGTCGAACAAGCAATAGAATATGTAAAGAAGAACAACGTTAAGTTCATAAGATTCCA
GTTCGTAGATATCTTAGGATTCCCAAAAAATGTCGCATACCCAGTTAAGGCTGGAGAAAAAGGAATCGAAGAATTAAGAG
AAATCTTTGAAAATGGAGTTTGGTTTGATGGTTCATCAATTACCGGTTTTGTTGGTATTGAAGAATCAGATATGTTATTA
AAACCAGATTTATCAACACTCTCTGTTTTACCATGGAGACCTGAAGAGAAAAGTGTTGCAAGAGTTATCTGTGATGTTTA
TAAAGATGAGAAGACACCATTCGAAGGAGACCCAAGAAGCAGATTAAAAGCTATTTTAGAAGAATTAAAGAAAGAAATGA
ATGGAGAATACTTTGTTGGTCCAGAACCAGAGTTCTTCTTGTTAAAGAGAGACCCACACAACCCACACAGATGGGTTCCT
GCTGACGATGGAGGTTACTTTGATGTTGAACCATTAGACGACGCTCCAGACATTAGAAGAGATATCGTCTTAGCTTTAGA
AAACCTCGGCTTCCACGTTGAGGCATCACACCACGAAGTCGCTCCAGGACAGCACGAAGTTGATTTCAAATTCGACAACG
CTTTAAAGACAGCTGATAGCGTTATAACATTCAAGATGACAATCAAAAACATTGCTAAGAAGCACGGTTTAAAAGCTACA
TTCATGCCAAAACCATTCTTTGGAATGAACGGAAACGGAATGCACTGCCACCAGAGTGTCTGGTTTAACGGAGAACCATC
ATTCTACGACCCAGAAGGGCCTTACAATGGATTAAGTGAAACATGTTTAAGCTACATTGCTGGAATCTTAAGCCACGCTA
AGGCATTAGTTGCTATAACAAACCCAACAGTTAATTCATACAAGAGATTAGTCCCAGGTTACGAAGCTCCTGTAAATATC
GCATGGGCAAACAAGAACAGAAGTGCTATCATCAGAGTCCCAGCTGCAAGAGGAAAAGCTACAAGAATCGAGTTCAGAGC
TCCAGACCCAACATGCAACCCATACTTAGCATTTGCATGTATGTTAGCTGCTGGATTAGATGGAATTAAGAAGAAGATGA
CAGCTCCAGAGCCAGTTGAGAGAAACATCTTCAAGATGTCAGAAGAAGAGAAAAAGCAGTTAGGAATTGAGTCAGTTCCT
GCAAACTTAGCTGCTGCATTGGATGAGTTAGAATGTGATGAAGTCTTGCAGAAAGCTTTAGGAAAACACATATATGAAAA
CTACATGGAAATCAAGAGAGCTGAGTGGGATGACTTCAGAACAGCAGTTACTGACTGGGAGACAGGTAAATACTTAATCT
ACTAA

Upstream 100 bases:

>100_bases
TATTTATGAATAAATTTTTATATTTTAATTTAAATTGAATATACCGTAAATTATATATATATGGAAGTAGGAAACTTACT
TCCAGAATTATTCAATCAAA

Downstream 100 bases:

>100_bases
AATTTCTTCTCTTTTACCTTTTTTTATTACAGCAAAATTCCAAAGATTCCAATTATTTAATTTTAATATAATTTTAAAAG
GTGATTATTATGAAGGCAAT

Product: glutamine synthetase GlnA

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MHWGMEMNVEQAIEYVKKNNVKFIRFQFVDILGFPKNVAYPVKAGEKGIEELREIFENGVWFDGSSITGFVGIEESDMLL
KPDLSTLSVLPWRPEEKSVARVICDVYKDEKTPFEGDPRSRLKAILEELKKEMNGEYFVGPEPEFFLLKRDPHNPHRWVP
ADDGGYFDVEPLDDAPDIRRDIVLALENLGFHVEASHHEVAPGQHEVDFKFDNALKTADSVITFKMTIKNIAKKHGLKAT
FMPKPFFGMNGNGMHCHQSVWFNGEPSFYDPEGPYNGLSETCLSYIAGILSHAKALVAITNPTVNSYKRLVPGYEAPVNI
AWANKNRSAIIRVPAARGKATRIEFRAPDPTCNPYLAFACMLAAGLDGIKKKMTAPEPVERNIFKMSEEEKKQLGIESVP
ANLAAALDELECDEVLQKALGKHIYENYMEIKRAEWDDFRTAVTDWETGKYLIY

Sequences:

>Translated_454_residues
MHWGMEMNVEQAIEYVKKNNVKFIRFQFVDILGFPKNVAYPVKAGEKGIEELREIFENGVWFDGSSITGFVGIEESDMLL
KPDLSTLSVLPWRPEEKSVARVICDVYKDEKTPFEGDPRSRLKAILEELKKEMNGEYFVGPEPEFFLLKRDPHNPHRWVP
ADDGGYFDVEPLDDAPDIRRDIVLALENLGFHVEASHHEVAPGQHEVDFKFDNALKTADSVITFKMTIKNIAKKHGLKAT
FMPKPFFGMNGNGMHCHQSVWFNGEPSFYDPEGPYNGLSETCLSYIAGILSHAKALVAITNPTVNSYKRLVPGYEAPVNI
AWANKNRSAIIRVPAARGKATRIEFRAPDPTCNPYLAFACMLAAGLDGIKKKMTAPEPVERNIFKMSEEEKKQLGIESVP
ANLAAALDELECDEVLQKALGKHIYENYMEIKRAEWDDFRTAVTDWETGKYLIY
>Mature_454_residues
MHWGMEMNVEQAIEYVKKNNVKFIRFQFVDILGFPKNVAYPVKAGEKGIEELREIFENGVWFDGSSITGFVGIEESDMLL
KPDLSTLSVLPWRPEEKSVARVICDVYKDEKTPFEGDPRSRLKAILEELKKEMNGEYFVGPEPEFFLLKRDPHNPHRWVP
ADDGGYFDVEPLDDAPDIRRDIVLALENLGFHVEASHHEVAPGQHEVDFKFDNALKTADSVITFKMTIKNIAKKHGLKAT
FMPKPFFGMNGNGMHCHQSVWFNGEPSFYDPEGPYNGLSETCLSYIAGILSHAKALVAITNPTVNSYKRLVPGYEAPVNI
AWANKNRSAIIRVPAARGKATRIEFRAPDPTCNPYLAFACMLAAGLDGIKKKMTAPEPVERNIFKMSEEEKKQLGIESVP
ANLAAALDELECDEVLQKALGKHIYENYMEIKRAEWDDFRTAVTDWETGKYLIY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Homo sapiens, GI221307485, Length=462, Percent_Identity=25.7575757575758, Blast_Score=122, Evalue=5e-28,
Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=40.9381663113006, Blast_Score=314, Evalue=6e-87,
Organism=Escherichia coli, GI87081870, Length=451, Percent_Identity=26.8292682926829, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI115532978, Length=330, Percent_Identity=24.5454545454545, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532980, Length=330, Percent_Identity=24.5454545454545, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281360453, Length=322, Percent_Identity=27.639751552795, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17136208, Length=322, Percent_Identity=27.639751552795, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580558, Length=322, Percent_Identity=27.639751552795, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): GLNA_METJA (Q60182)

Other databases:

- EMBL:   L77117
- PIR:   A64468
- RefSeq:   NP_248348.1
- ProteinModelPortal:   Q60182
- SMR:   Q60182
- GeneID:   1452249
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1346
- NMPDR:   fig|243232.1.peg.1385
- TIGR:   MJ1346
- HOGENOM:   HBG553755
- OMA:   KINGSGM
- ProtClustDB:   CLSK876540
- BioCyc:   MJAN243232:MJ_1346-MONOMER
- BRENDA:   6.3.1.2
- GO:   GO:0005737
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- Gene3D:   G3DSA:3.30.590.10
- TIGRFAMs:   TIGR00653

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N; SSF54368 Gln_synt_beta

EC number: =6.3.1.2

Molecular weight: Translated: 51399; Mature: 51399

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00180 GLNA_1; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHWGMEMNVEQAIEYVKKNNVKFIRFQFVDILGFPKNVAYPVKAGEKGIEELREIFENGV
CCCCCCCCHHHHHHHHHHCCCEEEEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
WFDGSSITGFVGIEESDMLLKPDLSTLSVLPWRPEEKSVARVICDVYKDEKTPFEGDPRS
EECCCCEEEEEECCCCCCEECCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHH
RLKAILEELKKEMNGEYFVGPEPEFFLLKRDPHNPHRWVPADDGGYFDVEPLDDAPDIRR
HHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCHHHH
DIVLALENLGFHVEASHHEVAPGQHEVDFKFDNALKTADSVITFKMTIKNIAKKHGLKAT
HHHHHHHHCCEEEECCCCCCCCCCCEEEEEECHHHHHHCCEEEEEHHHHHHHHHHCCEEE
FMPKPFFGMNGNGMHCHQSVWFNGEPSFYDPEGPYNGLSETCLSYIAGILSHAKALVAIT
ECCCCCCCCCCCCCEEECEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEE
NPTVNSYKRLVPGYEAPVNIAWANKNRSAIIRVPAARGKATRIEFRAPDPTCNPYLAFAC
CCCHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHH
MLAAGLDGIKKKMTAPEPVERNIFKMSEEEKKQLGIESVPANLAAALDELECDEVLQKAL
HHHHCHHHHHHHCCCCCCHHHHHHHCCHHHHHHCCCHHCCHHHHHHHHHCCHHHHHHHHH
GKHIYENYMEIKRAEWDDFRTAVTDWETGKYLIY
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEC
>Mature Secondary Structure
MHWGMEMNVEQAIEYVKKNNVKFIRFQFVDILGFPKNVAYPVKAGEKGIEELREIFENGV
CCCCCCCCHHHHHHHHHHCCCEEEEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
WFDGSSITGFVGIEESDMLLKPDLSTLSVLPWRPEEKSVARVICDVYKDEKTPFEGDPRS
EECCCCEEEEEECCCCCCEECCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHH
RLKAILEELKKEMNGEYFVGPEPEFFLLKRDPHNPHRWVPADDGGYFDVEPLDDAPDIRR
HHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCHHHH
DIVLALENLGFHVEASHHEVAPGQHEVDFKFDNALKTADSVITFKMTIKNIAKKHGLKAT
HHHHHHHHCCEEEECCCCCCCCCCCEEEEEECHHHHHHCCEEEEEHHHHHHHHHHCCEEE
FMPKPFFGMNGNGMHCHQSVWFNGEPSFYDPEGPYNGLSETCLSYIAGILSHAKALVAIT
ECCCCCCCCCCCCCEEECEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEE
NPTVNSYKRLVPGYEAPVNIAWANKNRSAIIRVPAARGKATRIEFRAPDPTCNPYLAFAC
CCCHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHH
MLAAGLDGIKKKMTAPEPVERNIFKMSEEEKKQLGIESVPANLAAALDELECDEVLQKAL
HHHHCHHHHHHHCCCCCCHHHHHHHCCHHHHHHCCCHHCCHHHHHHHHHCCHHHHHHHHH
GKHIYENYMEIKRAEWDDFRTAVTDWETGKYLIY
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087