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The map label for this gene is amtB [C]

Identifier: 15669534

GI number: 15669534

Start: 1289834

End: 1291096

Strand: Reverse

Name: amtB [C]

Synonym: MJ1343

Alternate gene names: 15669534

Gene position: 1291096-1289834 (Counterclockwise)

Preceding gene: 15669535

Following gene: 15669532

Centisome position: 77.54

GC content: 38.88

Gene sequence:

>1263_bases
TTGTGGGGTGAAAACATAGCTACTGCGGATTTGTTTGCGAATGCCACAGATATACATTCAATAGTTCAGGCATTGACCAC
CTTAGCAAATGCTTCAGATGTGTTCTTCCTTGTAGTAATGGGAGTTCTTGTCTTTATGATGCAGTGGGGCTTTGCGATGC
TTGAAGGTGGTCAGGTAAGGAAGAAAAATGTTAATAATGTTATGATGAAGAACATGGTTGATTGGTTGATTGGTTGTGTT
GCATGGTTATTCATTGGTGGAATTTTATGTTCAAAAGGTTTTGATTTATCTGCATTTATAGATTGGTGGAAACAAATACT
TGGAACAAACTGGCCAAATAATGGATTGGACTTAGCAAGCTGGTTCTTTGGTCTTGTCTTCTGTGCTACTGCTGCAACAA
TTGTCTCTGGAGGAGTTGCAGAGAGAATAAAATTCAGTGCTTATGTTCTAATTTCATTGATTATTACAGGTCTATTATAT
CCTCTCTTCGTATATTTAGGACCTTGGGGAGCAAGTATAGTTCCATGGCATGACTATGCTGGAAGTTTGGTTGTTCATGG
TTTAGGTGGTTTTTTAGCTTTGGGAGCAATTGCAGCATTAGGTCCAAGAATTGGAAGATTTGTTGATGGAAGACCAGTTC
CAATATTGGGACACAACATTCCAATGGCAGTATTTGGGGCATTTGCATTGGCAATTGGTTGGTATGGATTCAACGTAGGT
AGTTCATTGGCTTTAGGAGATATTTCAGGGCTTGTATGTGCTACAACTACAATGGCAATGGCTGGAGGAGGAATAGGGGC
ATTAATTGCTTCAAGAAATGATGTTCTATTTACAGCCAACGGAATAGTCGCTGGTTTAGTTGCAATCTGTTCAGGGACAG
ATGTTGTTAGCCCAATAGGTGGATTAATAATTGGTTTAATTGCTGGATTGCAAGTTCCAATTGTCTATAAACTTGTTGAA
AAAGCAGGATTGGATGATGTCTGTGGCGTAGTGCCTGTCCATGGAACTGCAGGTGTTATAGGAGCAATCTTAACTGGAAT
TTTAGGATTAAAAATATTTGGTGGAGCAGGAGGCGTTAGTTTAATAGACCAGATAATTGGAGCAGTATTTTGTATTATTT
ATGGAACAGGGCTTGGATATATTTTAGCGAAGATTGTTGGTATTGCATTAGGTGGATTAAGAGTTAGTGAAGAAGAAGAA
AAAATGGGATTGGATATGGCAGAACACAAAATGCCTGCTTATCCAGAAGAGACAGTTATCTAA

Upstream 100 bases:

>100_bases
AAAAATCTTCGTCATACCAGTAGAAAGAGTCGTAAGAGTAAGAACAAAAGAAGAGGGTAGAGATGTACTTTAAAAATTTA
ATTATGTAATTTAAAGAGAG

Downstream 100 bases:

>100_bases
AATTCTTAATTTATTTTAATTTATTTTTGGACAATAATTATTTAATCCTAAACCAACAATATCCGTTTTCTTTTATTATT
ACCTTATTTCCATCCCATAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MWGENIATADLFANATDIHSIVQALTTLANASDVFFLVVMGVLVFMMQWGFAMLEGGQVRKKNVNNVMMKNMVDWLIGCV
AWLFIGGILCSKGFDLSAFIDWWKQILGTNWPNNGLDLASWFFGLVFCATAATIVSGGVAERIKFSAYVLISLIITGLLY
PLFVYLGPWGASIVPWHDYAGSLVVHGLGGFLALGAIAALGPRIGRFVDGRPVPILGHNIPMAVFGAFALAIGWYGFNVG
SSLALGDISGLVCATTTMAMAGGGIGALIASRNDVLFTANGIVAGLVAICSGTDVVSPIGGLIIGLIAGLQVPIVYKLVE
KAGLDDVCGVVPVHGTAGVIGAILTGILGLKIFGGAGGVSLIDQIIGAVFCIIYGTGLGYILAKIVGIALGGLRVSEEEE
KMGLDMAEHKMPAYPEETVI

Sequences:

>Translated_420_residues
MWGENIATADLFANATDIHSIVQALTTLANASDVFFLVVMGVLVFMMQWGFAMLEGGQVRKKNVNNVMMKNMVDWLIGCV
AWLFIGGILCSKGFDLSAFIDWWKQILGTNWPNNGLDLASWFFGLVFCATAATIVSGGVAERIKFSAYVLISLIITGLLY
PLFVYLGPWGASIVPWHDYAGSLVVHGLGGFLALGAIAALGPRIGRFVDGRPVPILGHNIPMAVFGAFALAIGWYGFNVG
SSLALGDISGLVCATTTMAMAGGGIGALIASRNDVLFTANGIVAGLVAICSGTDVVSPIGGLIIGLIAGLQVPIVYKLVE
KAGLDDVCGVVPVHGTAGVIGAILTGILGLKIFGGAGGVSLIDQIIGAVFCIIYGTGLGYILAKIVGIALGGLRVSEEEE
KMGLDMAEHKMPAYPEETVI
>Mature_420_residues
MWGENIATADLFANATDIHSIVQALTTLANASDVFFLVVMGVLVFMMQWGFAMLEGGQVRKKNVNNVMMKNMVDWLIGCV
AWLFIGGILCSKGFDLSAFIDWWKQILGTNWPNNGLDLASWFFGLVFCATAATIVSGGVAERIKFSAYVLISLIITGLLY
PLFVYLGPWGASIVPWHDYAGSLVVHGLGGFLALGAIAALGPRIGRFVDGRPVPILGHNIPMAVFGAFALAIGWYGFNVG
SSLALGDISGLVCATTTMAMAGGGIGALIASRNDVLFTANGIVAGLVAICSGTDVVSPIGGLIIGLIAGLQVPIVYKLVE
KAGLDDVCGVVPVHGTAGVIGAILTGILGLKIFGGAGGVSLIDQIIGAVFCIIYGTGLGYILAKIVGIALGGLRVSEEEE
KMGLDMAEHKMPAYPEETVI

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family

Homologues:

Organism=Escherichia coli, GI1786656, Length=408, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17550182, Length=439, Percent_Identity=32.3462414578588, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17550180, Length=458, Percent_Identity=31.6593886462882, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32564042, Length=419, Percent_Identity=29.1169451073986, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=433, Percent_Identity=27.0207852193995, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=26.4423076923077, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325396, Length=419, Percent_Identity=26.491646778043, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324187, Length=438, Percent_Identity=24.8858447488584, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI161078314, Length=417, Percent_Identity=32.3741007194245, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1343_METJA (Q58739)

Other databases:

- EMBL:   L77117
- PIR:   F64467
- RefSeq:   NP_248345.1
- ProteinModelPortal:   Q58739
- SMR:   Q58739
- MINT:   MINT-4297916
- GeneID:   1452246
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1343
- NMPDR:   fig|243232.1.peg.1382
- TIGR:   MJ1343
- HOGENOM:   HBG677626
- OMA:   LAIGWYG
- ProtClustDB:   CLSK876539
- BioCyc:   MJAN243232:MJ_1343-MONOMER
- InterPro:   IPR001905
- InterPro:   IPR018047
- PANTHER:   PTHR11730
- TIGRFAMs:   TIGR00836

Pfam domain/function: PF00909 Ammonium_transp; SSF111352 RH_like_transpt

EC number: NA

Molecular weight: Translated: 43786; Mature: 43786

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe539340)-; HASH(0xcbbdea8)-; HASH(0xe899144)-; HASH(0xe006f20)-; HASH(0xdfde548)-; HASH(0xdd66c90)-; HASH(0xe88e42c)-; HASH(0xe75b738)-; HASH(0xe7ec88c)-; HASH(0xe79111c)-; HASH(0xe890c4c)-;

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWGENIATADLFANATDIHSIVQALTTLANASDVFFLVVMGVLVFMMQWGFAMLEGGQVR
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
KKNVNNVMMKNMVDWLIGCVAWLFIGGILCSKGFDLSAFIDWWKQILGTNWPNNGLDLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
WFFGLVFCATAATIVSGGVAERIKFSAYVLISLIITGLLYPLFVYLGPWGASIVPWHDYA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
GSLVVHGLGGFLALGAIAALGPRIGRFVDGRPVPILGHNIPMAVFGAFALAIGWYGFNVG
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCC
SSLALGDISGLVCATTTMAMAGGGIGALIASRNDVLFTANGIVAGLVAICSGTDVVSPIG
CCCCHHHHHHHHHHHHHHHHHCCCHHHHEECCCCEEEECCHHHHHHHHHHCCCCHHHHHH
GLIIGLIAGLQVPIVYKLVEKAGLDDVCGVVPVHGTAGVIGAILTGILGLKIFGGAGGVS
HHHHHHHHHCHHHHHHHHHHHCCCCHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCHH
LIDQIIGAVFCIIYGTGLGYILAKIVGIALGGLRVSEEEEKMGLDMAEHKMPAYPEETVI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHCCCCCCCCCCH
>Mature Secondary Structure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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087