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The map label for this gene is bioB

Identifier: 15669486

GI number: 15669486

Start: 1243510

End: 1244586

Strand: Reverse

Name: bioB

Synonym: MJ1296

Alternate gene names: 15669486

Gene position: 1244586-1243510 (Counterclockwise)

Preceding gene: 15669491

Following gene: 15669485

Centisome position: 74.75

GC content: 30.64

Gene sequence:

>1077_bases
GTGATTTTTATGGAGATTGAAACTTTTTTAAAAAAATCTCTAAAGAACAAAATAGATTTTGATGATGCCCTCTATTTATA
TAATAACTTCAGTGCTATAGATTTGTTATATTTGGCTTTTAAAATAAAAAATAGGATAAAAAATAATAGCAAAATTAAAT
TATGTGCTATAATAAATGCAAAAAGTGGAAAATGCAAAGAGGATTGTATTTTCTGCTCTCAATCAATTTATAGTAAATGC
AACATCCCAATATATCCATTAAAATCTAAAAAGGAGATTTTAGAGTATGCTAAAAAAATCATCGATGAATGTTCTAAAAT
TTCCTCATCCATAGAACGTGGGACATTAATTGGGGCTGAAAGCCCCAACTTAATGGACGTGGGGTATCCCAATAGGGGGT
TTCCCCTATGGGTAGAGAGATTCAGTATAGTAACAAGTGGCAAAAAAATTAATGATGATGAATTCATTGAAATTGTTGAA
GCTATAGAGCTTATAAAGGAAGAAACAAATTTAAAAGTGTGCTGTTCTTTGGGTTTATTGGATAGAGAAAAATTAAAAGA
ACTAAAAAAATTGGACGTTAGGATTCACAATAACTTAGAGGCATCAAAAAACTACTTTAAAAATATCTGTTCAACTCATA
GCTATGAAGATAAAGTAAAAGTTATAAAAGAGGCAAAAAAACTTGACTTAGAGGTTTGTAGTGGTGGAATATTTGGACTT
GGAGAGAGCGTAGAGGAGAGAATAAAGATGGCTTTTGAACTTAAAGAGTTAGGGGTTGATAGCGTTCCAATAAATATTTT
ACATCCAATTGAAGGAACTAAAGCTTATGAAAAAATAAAAAATGGAGAGATTAAGCCAATAAGTGTCTCAGATGCTTTGA
AATTGATAGCGTTATATAAAATAATTATGCCTTATGCAGAGATTAGATTGGCTGGTGGGAGAATATACAACTTAAGAGAC
TTCCAATCTTATGCCTTAATGGTCTTAGACGGATTAATGGTTGGGAATTATTTAACTACAAAGGGAAGATGTTTAGAGGA
TGATTTAAAGATGATTGCTGATTTCCACAGTTTATAA

Upstream 100 bases:

>100_bases
CCAGAGATGTGTTTTCCATTCTTCGATGCCCTCATCTTTATACTATACATAACAATCACCAATAAATTTTAAAAGTTAAT
GTTTATTTATTGTATATAGG

Downstream 100 bases:

>100_bases
AATGAGGTGATATTTTGAGATTTGATTTTCATACGCATACGGTTTTTAGTGATGGAGAGCTAATTCCTGCTGAATTAGTT
AGAAGGGCAAGGGTCTTAAA

Product: biotin synthetase BioB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MIFMEIETFLKKSLKNKIDFDDALYLYNNFSAIDLLYLAFKIKNRIKNNSKIKLCAIINAKSGKCKEDCIFCSQSIYSKC
NIPIYPLKSKKEILEYAKKIIDECSKISSSIERGTLIGAESPNLMDVGYPNRGFPLWVERFSIVTSGKKINDDEFIEIVE
AIELIKEETNLKVCCSLGLLDREKLKELKKLDVRIHNNLEASKNYFKNICSTHSYEDKVKVIKEAKKLDLEVCSGGIFGL
GESVEERIKMAFELKELGVDSVPINILHPIEGTKAYEKIKNGEIKPISVSDALKLIALYKIIMPYAEIRLAGGRIYNLRD
FQSYALMVLDGLMVGNYLTTKGRCLEDDLKMIADFHSL

Sequences:

>Translated_358_residues
MIFMEIETFLKKSLKNKIDFDDALYLYNNFSAIDLLYLAFKIKNRIKNNSKIKLCAIINAKSGKCKEDCIFCSQSIYSKC
NIPIYPLKSKKEILEYAKKIIDECSKISSSIERGTLIGAESPNLMDVGYPNRGFPLWVERFSIVTSGKKINDDEFIEIVE
AIELIKEETNLKVCCSLGLLDREKLKELKKLDVRIHNNLEASKNYFKNICSTHSYEDKVKVIKEAKKLDLEVCSGGIFGL
GESVEERIKMAFELKELGVDSVPINILHPIEGTKAYEKIKNGEIKPISVSDALKLIALYKIIMPYAEIRLAGGRIYNLRD
FQSYALMVLDGLMVGNYLTTKGRCLEDDLKMIADFHSL
>Mature_358_residues
MIFMEIETFLKKSLKNKIDFDDALYLYNNFSAIDLLYLAFKIKNRIKNNSKIKLCAIINAKSGKCKEDCIFCSQSIYSKC
NIPIYPLKSKKEILEYAKKIIDECSKISSSIERGTLIGAESPNLMDVGYPNRGFPLWVERFSIVTSGKKINDDEFIEIVE
AIELIKEETNLKVCCSLGLLDREKLKELKKLDVRIHNNLEASKNYFKNICSTHSYEDKVKVIKEAKKLDLEVCSGGIFGL
GESVEERIKMAFELKELGVDSVPINILHPIEGTKAYEKIKNGEIKPISVSDALKLIALYKIIMPYAEIRLAGGRIYNLRD
FQSYALMVLDGLMVGNYLTTKGRCLEDDLKMIADFHSL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=285, Percent_Identity=24.9122807017544, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321725, Length=329, Percent_Identity=25.531914893617, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_METJA (Q58692)

Other databases:

- EMBL:   L77117
- PIR:   G64461
- RefSeq:   NP_248296.1
- ProteinModelPortal:   Q58692
- SMR:   Q58692
- GeneID:   1452198
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1296
- NMPDR:   fig|243232.1.peg.1333
- TIGR:   MJ1296
- HOGENOM:   HBG405569
- OMA:   LEICSGG
- ProtClustDB:   CLSK2749974
- BioCyc:   MJAN243232:MJ_1296-MONOMER
- BRENDA:   2.8.1.6
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 40722; Mature: 40722

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFMEIETFLKKSLKNKIDFDDALYLYNNFSAIDLLYLAFKIKNRIKNNSKIKLCAIINA
CEEEHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
KSGKCKEDCIFCSQSIYSKCNIPIYPLKSKKEILEYAKKIIDECSKISSSIERGTLIGAE
CCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
SPNLMDVGYPNRGFPLWVERFSIVTSGKKINDDEFIEIVEAIELIKEETNLKVCCSLGLL
CCCEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCC
DREKLKELKKLDVRIHNNLEASKNYFKNICSTHSYEDKVKVIKEAKKLDLEVCSGGIFGL
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GESVEERIKMAFELKELGVDSVPINILHPIEGTKAYEKIKNGEIKPISVSDALKLIALYK
CHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHH
IIMPYAEIRLAGGRIYNLRDFQSYALMVLDGLMVGNYLTTKGRCLEDDLKMIADFHSL
HHHHHHHEEECCCEEEEHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIFMEIETFLKKSLKNKIDFDDALYLYNNFSAIDLLYLAFKIKNRIKNNSKIKLCAIINA
CEEEHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
KSGKCKEDCIFCSQSIYSKCNIPIYPLKSKKEILEYAKKIIDECSKISSSIERGTLIGAE
CCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
SPNLMDVGYPNRGFPLWVERFSIVTSGKKINDDEFIEIVEAIELIKEETNLKVCCSLGLL
CCCEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCC
DREKLKELKKLDVRIHNNLEASKNYFKNICSTHSYEDKVKVIKEAKKLDLEVCSGGIFGL
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GESVEERIKMAFELKELGVDSVPINILHPIEGTKAYEKIKNGEIKPISVSDALKLIALYK
CHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHH
IIMPYAEIRLAGGRIYNLRDFQSYALMVLDGLMVGNYLTTKGRCLEDDLKMIADFHSL
HHHHHHHEEECCCEEEEHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087