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The map label for this gene is pheT

Identifier: 15669296

GI number: 15669296

Start: 1047158

End: 1048804

Strand: Reverse

Name: pheT

Synonym: MJ1108

Alternate gene names: 15669296

Gene position: 1048804-1047158 (Counterclockwise)

Preceding gene: 15669298

Following gene: 15669295

Centisome position: 62.99

GC content: 31.15

Gene sequence:

>1647_bases
ATGCCAACAATAAATGTAAAAAAAGCTGATTTAGAGAGATTGGTTAATATGCCCTTAGAGGATGAATTTATTGAAGAGAA
ATTTCCAATGATGGGTGTTGAAGTTGAAGGAATCTTTGAAGAAGATGGAGAAAAAATTATTCAGTTCTCAATAAACCCAA
ATAGACCAGATTATTTAAGTGCTGAAGGTTTAGCAAGAGGTTTTAGGGGAATTATTGGAATAGAAACAGGATTAAAAAAA
TACGACATTGAGAGTTCAGATGTAAAATTATATGTTGAGAATGTTGAAACAAGACCATACATAGCAATGGCTTTGGTTAA
AGGGGTTATTGTTGATGATTATGTTTTAGAGAGCATAATTAACCTTCAAGAAAAGCTCCACTGGGTTATGGGAAGAGATA
GGAAAAAAGTGGCAATAGGAATTCATGATGCAGATAAAGTTAAGCCTCCATTCTACTACAAAGAAGTTAGTGGGGATGGG
ATTAAGTTTGTTCCATTAAATTCAGATGAGGAAATGACACCAAGAGAGATTTTAGAAAAACATGAAAAAGGAATAAAATA
TGCTCATTTAATCAAAGATGATAAGTTTCCAATAATATTAGATAGTGAAGGGGATGTTTTATCTATGCCACCAATAATTA
ATGGGGAATTAACAAGAGTTACAACTGAAACAAGGAATTTATTGATTGATGTTACTGGAACTGATAAATATGCAGTAGAA
AAAACTCTAAATATTATTGTTACTGCATTGGCAGAGAGAAAGTATGGAAAAATACATGCTGTTGAAGTAATTAAAGACAA
TCAAAGCACTATATATCCAAATTTAAAAGAGGATGTCTTAGAAACTACTTCTGAATACATAAACAAGGTTTTAGGAGCCA
ATCTAACTCCTGGGACTATAATAAACTACTTAAGAAGATGTAGATTAGACGCTCAATTTGTAGATAACAAAATAAAGGTT
TTCATCCCTGCCTATAGAGTTGATATCTTTGGAGAGATTGACATCGCTGAAGAAGTAGCTATTGCTTACGGATATAATAA
GTTCTCTGGAGAATATCCAATTATTGGAACTATTGGGGAACTTAACCAATTAGAAAAGAAATGTGACTTTATAAGAGAAA
TTATGGTTGGATTTGGATTCTATGAGGTTATAAATTTAATGCTTTCAAATGATGAGGTTTTATTTAAAAAGATGAGAATT
GAAGACAACAACTATATAGAAGTTTTAAAACCAGCATCTATAGAGCATAGAATCGTTAGAAAAAGTATCTTACCATTGCT
AATGGAAACTTTGAGGATAAATAAACATAAAGAGTTGCCACAAAAGATTTTTGAGATTGGAGATTGTGTTGTTATTGATG
AAAATGCTGAAACAAAATCAAGAGTTGTTAAAAAAATAGCTGGAGTTATTGTAGATAATGAAACAAACTTTAATGAGATA
AAGAGCTATGTTGAAGGTTTATTGAGAGAGCTTAAAATTGAGTATGAGCTTGATAATTTTGAACATCCATCATTCATTAA
AGGAAGATGTGCTAAAATATTGAAAGATGGCAAAATTATTGGCTACTTTGGAGAGATTCATCCAGAGGTTATTACCAACT
TTGAATTAGAATTCCCAGTTGTTGGATTTGAGTTAGAGATTGAATAA

Upstream 100 bases:

>100_bases
GTATATAAACATTTTGTGATAATAAAGCTTTATTAAGTCAATTATCCTATTATTACAACTAATTATTACAACTAAGATAA
AAAATACTGCTGGTGATAGG

Downstream 100 bases:

>100_bases
TGATAAAAGAGGATGAAACTCTTTGACCTACTTCAGTAAAATAAAATTTTTAAAAATTTTTAAGGAATTTCCTAAATTCT
AAATATATGAAATTTTTTGT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MPTINVKKADLERLVNMPLEDEFIEEKFPMMGVEVEGIFEEDGEKIIQFSINPNRPDYLSAEGLARGFRGIIGIETGLKK
YDIESSDVKLYVENVETRPYIAMALVKGVIVDDYVLESIINLQEKLHWVMGRDRKKVAIGIHDADKVKPPFYYKEVSGDG
IKFVPLNSDEEMTPREILEKHEKGIKYAHLIKDDKFPIILDSEGDVLSMPPIINGELTRVTTETRNLLIDVTGTDKYAVE
KTLNIIVTALAERKYGKIHAVEVIKDNQSTIYPNLKEDVLETTSEYINKVLGANLTPGTIINYLRRCRLDAQFVDNKIKV
FIPAYRVDIFGEIDIAEEVAIAYGYNKFSGEYPIIGTIGELNQLEKKCDFIREIMVGFGFYEVINLMLSNDEVLFKKMRI
EDNNYIEVLKPASIEHRIVRKSILPLLMETLRINKHKELPQKIFEIGDCVVIDENAETKSRVVKKIAGVIVDNETNFNEI
KSYVEGLLRELKIEYELDNFEHPSFIKGRCAKILKDGKIIGYFGEIHPEVITNFELEFPVVGFELEIE

Sequences:

>Translated_548_residues
MPTINVKKADLERLVNMPLEDEFIEEKFPMMGVEVEGIFEEDGEKIIQFSINPNRPDYLSAEGLARGFRGIIGIETGLKK
YDIESSDVKLYVENVETRPYIAMALVKGVIVDDYVLESIINLQEKLHWVMGRDRKKVAIGIHDADKVKPPFYYKEVSGDG
IKFVPLNSDEEMTPREILEKHEKGIKYAHLIKDDKFPIILDSEGDVLSMPPIINGELTRVTTETRNLLIDVTGTDKYAVE
KTLNIIVTALAERKYGKIHAVEVIKDNQSTIYPNLKEDVLETTSEYINKVLGANLTPGTIINYLRRCRLDAQFVDNKIKV
FIPAYRVDIFGEIDIAEEVAIAYGYNKFSGEYPIIGTIGELNQLEKKCDFIREIMVGFGFYEVINLMLSNDEVLFKKMRI
EDNNYIEVLKPASIEHRIVRKSILPLLMETLRINKHKELPQKIFEIGDCVVIDENAETKSRVVKKIAGVIVDNETNFNEI
KSYVEGLLRELKIEYELDNFEHPSFIKGRCAKILKDGKIIGYFGEIHPEVITNFELEFPVVGFELEIE
>Mature_547_residues
PTINVKKADLERLVNMPLEDEFIEEKFPMMGVEVEGIFEEDGEKIIQFSINPNRPDYLSAEGLARGFRGIIGIETGLKKY
DIESSDVKLYVENVETRPYIAMALVKGVIVDDYVLESIINLQEKLHWVMGRDRKKVAIGIHDADKVKPPFYYKEVSGDGI
KFVPLNSDEEMTPREILEKHEKGIKYAHLIKDDKFPIILDSEGDVLSMPPIINGELTRVTTETRNLLIDVTGTDKYAVEK
TLNIIVTALAERKYGKIHAVEVIKDNQSTIYPNLKEDVLETTSEYINKVLGANLTPGTIINYLRRCRLDAQFVDNKIKVF
IPAYRVDIFGEIDIAEEVAIAYGYNKFSGEYPIIGTIGELNQLEKKCDFIREIMVGFGFYEVINLMLSNDEVLFKKMRIE
DNNYIEVLKPASIEHRIVRKSILPLLMETLRINKHKELPQKIFEIGDCVVIDENAETKSRVVKKIAGVIVDNETNFNEIK
SYVEGLLRELKIEYELDNFEHPSFIKGRCAKILKDGKIIGYFGEIHPEVITNFELEFPVVGFELEIE

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B5 domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=585, Percent_Identity=31.6239316239316, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI24308207, Length=185, Percent_Identity=29.1891891891892, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788006, Length=393, Percent_Identity=23.6641221374046, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17533403, Length=591, Percent_Identity=31.4720812182741, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17510303, Length=227, Percent_Identity=24.6696035242291, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323089, Length=596, Percent_Identity=29.3624161073826, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI21355211, Length=586, Percent_Identity=31.2286689419795, Blast_Score=262, Evalue=4e-70,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_METJA (Q58508)

Other databases:

- EMBL:   L77117
- PIR:   C64438
- RefSeq:   NP_248101.1
- ProteinModelPortal:   Q58508
- SMR:   Q58508
- GeneID:   1452005
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1108
- NMPDR:   fig|243232.1.peg.1138
- TIGR:   MJ1108
- HOGENOM:   HBG497667
- OMA:   LLTRMCL
- ProtClustDB:   PRK09616
- BioCyc:   MJAN243232:MJ_1108-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00284
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR004531
- InterPro:   IPR020825
- InterPro:   IPR022918
- InterPro:   IPR002319
- Gene3D:   G3DSA:3.30.56.20
- SMART:   SM00873
- SMART:   SM00874
- TIGRFAMs:   TIGR00471

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF01409 tRNA-synt_2d; SSF56037 B3_4; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 62698; Mature: 62567

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS51483 B5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTINVKKADLERLVNMPLEDEFIEEKFPMMGVEVEGIFEEDGEKIIQFSINPNRPDYLS
CCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCC
AEGLARGFRGIIGIETGLKKYDIESSDVKLYVENVETRPYIAMALVKGVIVDDYVLESII
HHHHHHHHCCEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
NLQEKLHWVMGRDRKKVAIGIHDADKVKPPFYYKEVSGDGIKFVPLNSDEEMTPREILEK
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHH
HEKGIKYAHLIKDDKFPIILDSEGDVLSMPPIINGELTRVTTETRNLLIDVTGTDKYAVE
HHCCCEEEEEECCCCCCEEECCCCCEEECCCCCCCCEEEEEECCCEEEEEEECCCCHHHH
KTLNIIVTALAERKYGKIHAVEVIKDNQSTIYPNLKEDVLETTSEYINKVLGANLTPGTI
HHHHHHHHHHHHCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHCCCCCHHHH
INYLRRCRLDAQFVDNKIKVFIPAYRVDIFGEIDIAEEVAIAYGYNKFSGEYPIIGTIGE
HHHHHHHCCCHHHHCCEEEEEEEEEEEEEEECCCHHHHHHHHCCCCCCCCCCCEEECHHH
LNQLEKKCDFIREIMVGFGFYEVINLMLSNDEVLFKKMRIEDNNYIEVLKPASIEHRIVR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCHHHHHHH
KSILPLLMETLRINKHKELPQKIFEIGDCVVIDENAETKSRVVKKIAGVIVDNETNFNEI
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCEEECCCCCHHHH
KSYVEGLLRELKIEYELDNFEHPSFIKGRCAKILKDGKIIGYFGEIHPEVITNFELEFPV
HHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHCCEEEECCE
VGFELEIE
EEEEEEEC
>Mature Secondary Structure 
PTINVKKADLERLVNMPLEDEFIEEKFPMMGVEVEGIFEEDGEKIIQFSINPNRPDYLS
CCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCC
AEGLARGFRGIIGIETGLKKYDIESSDVKLYVENVETRPYIAMALVKGVIVDDYVLESII
HHHHHHHHCCEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
NLQEKLHWVMGRDRKKVAIGIHDADKVKPPFYYKEVSGDGIKFVPLNSDEEMTPREILEK
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHH
HEKGIKYAHLIKDDKFPIILDSEGDVLSMPPIINGELTRVTTETRNLLIDVTGTDKYAVE
HHCCCEEEEEECCCCCCEEECCCCCEEECCCCCCCCEEEEEECCCEEEEEEECCCCHHHH
KTLNIIVTALAERKYGKIHAVEVIKDNQSTIYPNLKEDVLETTSEYINKVLGANLTPGTI
HHHHHHHHHHHHCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHCCCCCHHHH
INYLRRCRLDAQFVDNKIKVFIPAYRVDIFGEIDIAEEVAIAYGYNKFSGEYPIIGTIGE
HHHHHHHCCCHHHHCCEEEEEEEEEEEEEEECCCHHHHHHHHCCCCCCCCCCCEEECHHH
LNQLEKKCDFIREIMVGFGFYEVINLMLSNDEVLFKKMRIEDNNYIEVLKPASIEHRIVR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCHHHHHHH
KSILPLLMETLRINKHKELPQKIFEIGDCVVIDENAETKSRVVKKIAGVIVDNETNFNEI
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCEEECCCCCHHHH
KSYVEGLLRELKIEYELDNFEHPSFIKGRCAKILKDGKIIGYFGEIHPEVITNFELEFPV
HHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHCCEEEECCE
VGFELEIE
EEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087