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The map label for this gene is rffG [C]

Identifier: 15669244

GI number: 15669244

Start: 993648

End: 994628

Strand: Direct

Name: rffG [C]

Synonym: MJ1055

Alternate gene names: 15669244

Gene position: 993648-994628 (Clockwise)

Preceding gene: 15669243

Following gene: 15669245

Centisome position: 59.68

GC content: 29.77

Gene sequence:

>981_bases
ATGAAATATAAAAATATCTTAGTTACTGGAAGTGCCGGTTTTATTGGTTTCCATCTAAGTAAATATTTAATGGATAACTA
TGAAGATCTAAAGGTTATTGGAATAGATAATTTAAATAACTACTATAACCCAGTTTTAAAAGAAAAAAGGAATGAAATTT
TAAAAAATTATGAAAATTATACGTTTATAAAATTGGATTTTTCAGATTGGGATGATTTAGTTGAAAATTTAAAAGATAAA
GAGATTGATTTGATTGTGCATTTAGGAGCACAGGCAGGAGTTAGATATTCTCTACAAAACCCATGGGCTTATATAAAATC
CAATGAAATGGGAACATTAAACATCTTCGAATTTGCAAGAAGATTTGATATTGAGAAGGTTGTTTATGCTTCCTCTTCTT
CTGTCTATGGAGGGAATAGGAAGATTCCTTTTAGTGAAGATGATAGAGTGGATAAACCAATCTCTCTATATGCCTCAACA
AAGAGAAGTAATGAGTTAATGGCTCATGTATATCATCATCTATATGGTATTAAAATGATTGGTTTAAGGTTTTTTACTGT
TTATGGAGAGTATGGAAGACCAGATATGGCTTACTTCAAGTTTGCAAAAAACATTTTATTGGGTAAGGAGATTGAGGTCT
ATAACTATGGAAATATGGAGAGGGACTTTACTTATATTTCTGATGTTGTGGATGGAATATTGAGAGCTATTAAAAAGGAC
TTTGATTATGAGATTTTTAATTTGGGTAATTCTAAACCAGTTAAGTTGATGTATTTTATTGAGTTAATTGAAAAATATCT
CAACAAAAAAGCAAAAAAGAAATTTTTGCCAATGCAGGATGGAGATGTTTTAAGAACCTATGCTGATTTGAGTAAGAGCG
AAAAGCTGTTGGGGTATAAACCAAAAGTTACTATTGAGGAGGGTTTAAAGAGATTTTGTAACTGGTTTTTGGAAAATAAA
GATTGGCTTTTAAGATTATAA

Upstream 100 bases:

>100_bases
TTGGAAATTTAGTTAAAGAAAAAGTAGTATTCGATGGAAGAAATATTTTAGATGTTGAAAAGATTAAAAAGTTGGGATTT
AAATATTATGGAGTGGGAAG

Downstream 100 bases:

>100_bases
TTTTTTTGGTGGAATGATGTGTGGGATAAATGGGATTATTAGATTTGGTAAAGAAGTTATAAAAGAAGAAATTAATAAGA
TGAATAAAGCAATTAAACAT

Product: capsular polysaccharide biosynthesis protein I

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKYKNILVTGSAGFIGFHLSKYLMDNYEDLKVIGIDNLNNYYNPVLKEKRNEILKNYENYTFIKLDFSDWDDLVENLKDK
EIDLIVHLGAQAGVRYSLQNPWAYIKSNEMGTLNIFEFARRFDIEKVVYASSSSVYGGNRKIPFSEDDRVDKPISLYAST
KRSNELMAHVYHHLYGIKMIGLRFFTVYGEYGRPDMAYFKFAKNILLGKEIEVYNYGNMERDFTYISDVVDGILRAIKKD
FDYEIFNLGNSKPVKLMYFIELIEKYLNKKAKKKFLPMQDGDVLRTYADLSKSEKLLGYKPKVTIEEGLKRFCNWFLENK
DWLLRL

Sequences:

>Translated_326_residues
MKYKNILVTGSAGFIGFHLSKYLMDNYEDLKVIGIDNLNNYYNPVLKEKRNEILKNYENYTFIKLDFSDWDDLVENLKDK
EIDLIVHLGAQAGVRYSLQNPWAYIKSNEMGTLNIFEFARRFDIEKVVYASSSSVYGGNRKIPFSEDDRVDKPISLYAST
KRSNELMAHVYHHLYGIKMIGLRFFTVYGEYGRPDMAYFKFAKNILLGKEIEVYNYGNMERDFTYISDVVDGILRAIKKD
FDYEIFNLGNSKPVKLMYFIELIEKYLNKKAKKKFLPMQDGDVLRTYADLSKSEKLLGYKPKVTIEEGLKRFCNWFLENK
DWLLRL
>Mature_326_residues
MKYKNILVTGSAGFIGFHLSKYLMDNYEDLKVIGIDNLNNYYNPVLKEKRNEILKNYENYTFIKLDFSDWDDLVENLKDK
EIDLIVHLGAQAGVRYSLQNPWAYIKSNEMGTLNIFEFARRFDIEKVVYASSSSVYGGNRKIPFSEDDRVDKPISLYAST
KRSNELMAHVYHHLYGIKMIGLRFFTVYGEYGRPDMAYFKFAKNILLGKEIEVYNYGNMERDFTYISDVVDGILRAIKKD
FDYEIFNLGNSKPVKLMYFIELIEKYLNKKAKKKFLPMQDGDVLRTYADLSKSEKLLGYKPKVTIEEGLKRFCNWFLENK
DWLLRL

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily

Homologues:

Organism=Homo sapiens, GI42516563, Length=323, Percent_Identity=31.8885448916409, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI7657641, Length=322, Percent_Identity=30.1242236024845, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI56237023, Length=335, Percent_Identity=24.1791044776119, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI56118217, Length=335, Percent_Identity=24.1791044776119, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI189083684, Length=335, Percent_Identity=24.1791044776119, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI48994969, Length=346, Percent_Identity=26.5895953757225, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788353, Length=344, Percent_Identity=25.2906976744186, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786974, Length=331, Percent_Identity=26.2839879154079, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1790049, Length=340, Percent_Identity=27.6470588235294, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788589, Length=356, Percent_Identity=22.1910112359551, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=327, Percent_Identity=25.6880733944954, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17539532, Length=324, Percent_Identity=28.0864197530864, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71982038, Length=346, Percent_Identity=25.4335260115607, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=346, Percent_Identity=25.1445086705202, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI115532424, Length=323, Percent_Identity=21.0526315789474, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=351, Percent_Identity=25.6410256410256, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356223, Length=322, Percent_Identity=31.9875776397516, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI19923002, Length=352, Percent_Identity=25, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1055_METJA (Q58455)

Other databases:

- EMBL:   L77117
- PIR:   F64431
- RefSeq:   NP_248049.1
- ProteinModelPortal:   Q58455
- GeneID:   1451952
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1055
- NMPDR:   fig|243232.1.peg.1086
- TIGR:   MJ1055
- HOGENOM:   HBG755066
- OMA:   LENPWAY
- ProtClustDB:   CLSK796709
- BioCyc:   MJAN243232:MJ_1055-MONOMER
- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR01713

Pfam domain/function: PF01370 Epimerase

EC number: 4.2.1.46

Molecular weight: Translated: 38370; Mature: 38370

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: ACT_SITE 157-157 BINDING 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKNILVTGSAGFIGFHLSKYLMDNYEDLKVIGIDNLNNYYNPVLKEKRNEILKNYENY
CCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCE
TFIKLDFSDWDDLVENLKDKEIDLIVHLGAQAGVRYSLQNPWAYIKSNEMGTLNIFEFAR
EEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCEEEEECCCCCEEHHHHHHH
RFDIEKVVYASSSSVYGGNRKIPFSEDDRVDKPISLYASTKRSNELMAHVYHHLYGIKMI
HCCHHHEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
GLRFFTVYGEYGRPDMAYFKFAKNILLGKEIEVYNYGNMERDFTYISDVVDGILRAIKKD
HEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
FDYEIFNLGNSKPVKLMYFIELIEKYLNKKAKKKFLPMQDGDVLRTYADLSKSEKLLGYK
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHCCCC
PKVTIEEGLKRFCNWFLENKDWLLRL
CCEEHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MKYKNILVTGSAGFIGFHLSKYLMDNYEDLKVIGIDNLNNYYNPVLKEKRNEILKNYENY
CCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCE
TFIKLDFSDWDDLVENLKDKEIDLIVHLGAQAGVRYSLQNPWAYIKSNEMGTLNIFEFAR
EEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCEEEEECCCCCEEHHHHHHH
RFDIEKVVYASSSSVYGGNRKIPFSEDDRVDKPISLYASTKRSNELMAHVYHHLYGIKMI
HCCHHHEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
GLRFFTVYGEYGRPDMAYFKFAKNILLGKEIEVYNYGNMERDFTYISDVVDGILRAIKKD
HEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
FDYEIFNLGNSKPVKLMYFIELIEKYLNKKAKKKFLPMQDGDVLRTYADLSKSEKLLGYK
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHCCCC
PKVTIEEGLKRFCNWFLENKDWLLRL
CCEEHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087