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The map label for this gene is ilvE

Identifier: 15669197

GI number: 15669197

Start: 938225

End: 939091

Strand: Reverse

Name: ilvE

Synonym: MJ1008

Alternate gene names: 15669197

Gene position: 939091-938225 (Counterclockwise)

Preceding gene: 15669198

Following gene: 15669188

Centisome position: 56.4

GC content: 34.26

Gene sequence:

>867_bases
ATGAAAATCTACTTAAACGGAAAGTTTGTTGATGAAAAAGATGCAAAGGTTTCTGTGTTTGACCACGGTTTATTATATGG
AGATGGAGTTTTTGAAGGAATTAGGGCTTATGATGGCGTTGTTTTTATGTTGAAGGAGCATATAGACAGATTGTATGATT
CAGCAAAATCTCTCTGTATAGATATCCCACTAACAAAAGAAGAGATGATTGATGTTGTTTTAGAGACATTGAGAGTTAAT
AATCTGAGAGATGCATATATAAGATTAGTTGTTACAAGAGGAGTTGGTGATTTAGGGTTAGACCCAAGAAAGTGTGGAAA
GCCAACTATTTTCTGTATAGCAATTCCTATGCCTCCTTTATTAGGGGAGGATGGAATCAGGGCTATAACCGTTTCAGTTA
GAAGACTGCCAGTAGATGTTTTGAATCCAGCAGTTAAATCCCTCAACTACTTAAACAGCGTCTTAGCAAAGATTCAGGCA
AACTATGCTGGAGTTGATGAGGCATTTTTATTGGATGATAAAGGTTTTGTTGTTGAAGGAACTGGAGATAACATATTTAT
AGTTAAAAATGGAGTTTTAAAAACTCCCCCAGTTTATCAGAGTATCTTAAAAGGAATCACAAGGGATGTTGTCATAAAAT
TAGCTAAGGAAGAAGGAATAGAGGTTGTTGAAGAACCTTTAACTTTACATGACTTATACACTGCCGATGAACTATTTATC
ACTGGAACAGCTGCTGAAATAGTCCCTGTTTTTGAGATAGATGGTAGAGTAATAAATAATAAACAAGTTGGAGAAATCAC
TAAAAAATTAAAAGAGAAGTTTAAAGATATTAGAACCAAATGGGGAATAAAGGTTTATGATGAATAA

Upstream 100 bases:

>100_bases
ATAAGTGAATAGACTCTATTTTTATTTTTTGCAAATAGACAATTTTATATATTAAATATTCATTTTATTTTTATTTTGAC
AATTTAACAAAGGTGGTCTT

Downstream 100 bases:

>100_bases
AAAATAAAAAAGTTAAAAAATTCAATTTATTTTTTTATTTTATCCATTTATTTCCTAATTATTGCCATTGCTAAAACTCC
CTCAATATCAACTGAATCTA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKIYLNGKFVDEKDAKVSVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCIDIPLTKEEMIDVVLETLRVN
NLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPLLGEDGIRAITVSVRRLPVDVLNPAVKSLNYLNSVLAKIQA
NYAGVDEAFLLDDKGFVVEGTGDNIFIVKNGVLKTPPVYQSILKGITRDVVIKLAKEEGIEVVEEPLTLHDLYTADELFI
TGTAAEIVPVFEIDGRVINNKQVGEITKKLKEKFKDIRTKWGIKVYDE

Sequences:

>Translated_288_residues
MKIYLNGKFVDEKDAKVSVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCIDIPLTKEEMIDVVLETLRVN
NLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPLLGEDGIRAITVSVRRLPVDVLNPAVKSLNYLNSVLAKIQA
NYAGVDEAFLLDDKGFVVEGTGDNIFIVKNGVLKTPPVYQSILKGITRDVVIKLAKEEGIEVVEEPLTLHDLYTADELFI
TGTAAEIVPVFEIDGRVINNKQVGEITKKLKEKFKDIRTKWGIKVYDE
>Mature_288_residues
MKIYLNGKFVDEKDAKVSVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCIDIPLTKEEMIDVVLETLRVN
NLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPLLGEDGIRAITVSVRRLPVDVLNPAVKSLNYLNSVLAKIQA
NYAGVDEAFLLDDKGFVVEGTGDNIFIVKNGVLKTPPVYQSILKGITRDVVIKLAKEEGIEVVEEPLTLHDLYTADELFI
TGTAAEIVPVFEIDGRVINNKQVGEITKKLKEKFKDIRTKWGIKVYDE

Specific function: Acts on leucine, isoleucine and valine

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Escherichia coli, GI48994963, Length=294, Percent_Identity=41.8367346938776, Blast_Score=213, Evalue=9e-57,
Organism=Escherichia coli, GI1787338, Length=245, Percent_Identity=29.3877551020408, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ILVE_METJA (Q58414)

Other databases:

- EMBL:   L77117
- PIR:   G64425
- RefSeq:   NP_248002.1
- ProteinModelPortal:   Q58414
- SMR:   Q58414
- GeneID:   1451905
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1008
- NMPDR:   fig|243232.1.peg.1039
- TIGR:   MJ1008
- HOGENOM:   HBG617123
- OMA:   AMYGDLY
- ProtClustDB:   PRK08320
- BioCyc:   MJAN243232:MJ_1008-MONOMER
- BRENDA:   2.6.1.42
- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785
- PANTHER:   PTHR11825
- TIGRFAMs:   TIGR01122

Pfam domain/function: PF01063 Aminotran_4; SSF56752 Aminotrans_IV

EC number: =2.6.1.42

Molecular weight: Translated: 32097; Mature: 32097

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLNGKFVDEKDAKVSVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCI
CEEEECCEEECCCCCEEEEEECCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEE
DIPLTKEEMIDVVLETLRVNNLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPL
ECCCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCHHHCCCCEEEEEEECCCCC
LGEDGIRAITVSVRRLPVDVLNPAVKSLNYLNSVLAKIQANYAGVDEAFLLDDKGFVVEG
CCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEC
TGDNIFIVKNGVLKTPPVYQSILKGITRDVVIKLAKEEGIEVVEEPLTLHDLYTADELFI
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCEEEE
TGTAAEIVPVFEIDGRVINNKQVGEITKKLKEKFKDIRTKWGIKVYDE
EECHHHEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCEEEECC
>Mature Secondary Structure
MKIYLNGKFVDEKDAKVSVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCI
CEEEECCEEECCCCCEEEEEECCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEE
DIPLTKEEMIDVVLETLRVNNLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPL
ECCCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCHHHCCCCEEEEEEECCCCC
LGEDGIRAITVSVRRLPVDVLNPAVKSLNYLNSVLAKIQANYAGVDEAFLLDDKGFVVEG
CCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEC
TGDNIFIVKNGVLKTPPVYQSILKGITRDVVIKLAKEEGIEVVEEPLTLHDLYTADELFI
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCEEEE
TGTAAEIVPVFEIDGRVINNKQVGEITKKLKEKFKDIRTKWGIKVYDE
EECHHHEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087