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The map label for this gene is cysH [C]

Identifier: 15669163

GI number: 15669163

Start: 906492

End: 907727

Strand: Reverse

Name: cysH [C]

Synonym: MJ0973

Alternate gene names: 15669163

Gene position: 907727-906492 (Counterclockwise)

Preceding gene: 15669164

Following gene: 15669160

Centisome position: 54.52

GC content: 27.75

Gene sequence:

>1236_bases
ATGGACGATAAATTTGCCTCTAAGTTTGAGATAGATGTTTTAAACAAACTGCTCAATAAAAACTTCTCCTATGATTTAGC
AATTATTTTAAAGAAGATTGGTGGCTTAGATTACAGAAAAAAAGTTTTTATTAATGGAGAGTGTATAGGCATCTTAGAAT
TTGATTTAATTGATTTGGATTGGAAGTTTCATCCTTATGCCTCTTATTATTTAATAGAAGAACCAAAAATTAAAATAAAA
CCAACAAAGAGAAAGCTAAAAGGCAAAAAAGTGCCAGTTGATTTAATTGAAAATGCTGAAGAGCTAAAAGATATCAATGA
GAATGATTATGTTGGTGTTGAAGTAGGAAATTATGTTGGCGTAGCAGTTAAAAAAGGAGATACAATAAAAATTAAGGACT
TAACTTTAAAGAAAGAGCTTAGATTTGAAAAGATTGAAGATTATCTAAGAAAAAACAAAGATAGGATTGAAAAATTAGAG
AAAAAATCCCTATCAATCATAAAAAAATACTATGAAATGTGTAAAAATAAGAATTATGCTATAAATACCTCTTTTAGTGG
TGGGAAGGATTCTTCTGTCTCTACTTTATTAGCTAACAAAGTTATAGATGATTTAGAAGTCATCTTTATAGATACCGGCT
TAGAATTTAAAGATACTATAGACTTTGTAAAAAAATTTGCTAAAAAGTATGATTTAAACTTAGTTGTTTTAAAAGGCAAA
AACTTTTGGGAATATCTGGAAAAAGAAGGTATTCCTACAAAAGATTATAGATGGTGTAATAGTGTTTGCAAATTAGAGCC
GTTAAAAGAGTATTTAAAGAAATATAAAAGAGTTTATACAATTGATGGCTCAAGGAGATATGAAAGCTTTACAAGAGAAA
AATTAACTTATGAAAGAAAAAGTGGCTTTATTGAAAATCAGATAAACATCTTCCCAATATTGGATTGGAGAGGAACTGAT
GTCTGGAGCTGGATATATCTAAATGATGTTATCTATAATGAACTCTATGATAAAGGATTTGAAAGAATTGGTTGTTATAT
GTGTCCAGCTGCTTTAAATGCTGAATTTTTGAGAGTTAAAGAACTTTATCCAGAGTTGTTTAATAAATGGGTTGATGTTT
TAAAAAGATTTGGTTATGATGAGGATGAGATTTTAAGAGGATTTTGGAGATGGAAAGAATTACCACCAAAAATGAAAGAA
TTAAAGAAAATATTAGAAAATAAAGAAAAAAAGTAA

Upstream 100 bases:

>100_bases
CTCAACATGTAAGTTTTATAAAATCAATAAAATATGTAGCTTTGACGATGATTTTAAAAGAGTAGATTTCTTAGAAATTA
TTGAAATTTAAAGTGATAAA

Downstream 100 bases:

>100_bases
TTTATTGAACGCTAATGCTATTATATAGCCCAACTATTCCAGTAAATATTATTTGCACTGAGATAGCTACTAATAGCAAT
CCCATAATTCTTACAAAGGC

Product: hypothetical protein

Products: H+; Adenosine 3',5'-bisphosphate; Sulfite; Oxidized thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MDDKFASKFEIDVLNKLLNKNFSYDLAIILKKIGGLDYRKKVFINGECIGILEFDLIDLDWKFHPYASYYLIEEPKIKIK
PTKRKLKGKKVPVDLIENAEELKDINENDYVGVEVGNYVGVAVKKGDTIKIKDLTLKKELRFEKIEDYLRKNKDRIEKLE
KKSLSIIKKYYEMCKNKNYAINTSFSGGKDSSVSTLLANKVIDDLEVIFIDTGLEFKDTIDFVKKFAKKYDLNLVVLKGK
NFWEYLEKEGIPTKDYRWCNSVCKLEPLKEYLKKYKRVYTIDGSRRYESFTREKLTYERKSGFIENQINIFPILDWRGTD
VWSWIYLNDVIYNELYDKGFERIGCYMCPAALNAEFLRVKELYPELFNKWVDVLKRFGYDEDEILRGFWRWKELPPKMKE
LKKILENKEKK

Sequences:

>Translated_411_residues
MDDKFASKFEIDVLNKLLNKNFSYDLAIILKKIGGLDYRKKVFINGECIGILEFDLIDLDWKFHPYASYYLIEEPKIKIK
PTKRKLKGKKVPVDLIENAEELKDINENDYVGVEVGNYVGVAVKKGDTIKIKDLTLKKELRFEKIEDYLRKNKDRIEKLE
KKSLSIIKKYYEMCKNKNYAINTSFSGGKDSSVSTLLANKVIDDLEVIFIDTGLEFKDTIDFVKKFAKKYDLNLVVLKGK
NFWEYLEKEGIPTKDYRWCNSVCKLEPLKEYLKKYKRVYTIDGSRRYESFTREKLTYERKSGFIENQINIFPILDWRGTD
VWSWIYLNDVIYNELYDKGFERIGCYMCPAALNAEFLRVKELYPELFNKWVDVLKRFGYDEDEILRGFWRWKELPPKMKE
LKKILENKEKK
>Mature_411_residues
MDDKFASKFEIDVLNKLLNKNFSYDLAIILKKIGGLDYRKKVFINGECIGILEFDLIDLDWKFHPYASYYLIEEPKIKIK
PTKRKLKGKKVPVDLIENAEELKDINENDYVGVEVGNYVGVAVKKGDTIKIKDLTLKKELRFEKIEDYLRKNKDRIEKLE
KKSLSIIKKYYEMCKNKNYAINTSFSGGKDSSVSTLLANKVIDDLEVIFIDTGLEFKDTIDFVKKFAKKYDLNLVVLKGK
NFWEYLEKEGIPTKDYRWCNSVCKLEPLKEYLKKYKRVYTIDGSRRYESFTREKLTYERKSGFIENQINIFPILDWRGTD
VWSWIYLNDVIYNELYDKGFERIGCYMCPAALNAEFLRVKELYPELFNKWVDVLKRFGYDEDEILRGFWRWKELPPKMKE
LKKILENKEKK

Specific function: Reduction Of Activated Sulfate Into Sulfite. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PAPS reductase family

Homologues:

Organism=Escherichia coli, GI1789121, Length=176, Percent_Identity=26.7045454545455, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y973_METJA (Q58383)

Other databases:

- EMBL:   L77117
- PIR:   E64421
- RefSeq:   NP_247968.1
- ProteinModelPortal:   Q58383
- SMR:   Q58383
- GeneID:   1451871
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0973
- NMPDR:   fig|243232.1.peg.1005
- TIGR:   MJ0973
- HOGENOM:   HBG541025
- OMA:   FERIGCY
- ProtClustDB:   PRK08557
- BioCyc:   MJAN243232:MJ_0973-MONOMER
- InterPro:   IPR002500
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620

Pfam domain/function: PF01507 PAPS_reduct

EC number: 1.8.4.8 [C]

Molecular weight: Translated: 48904; Mature: 48904

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDKFASKFEIDVLNKLLNKNFSYDLAIILKKIGGLDYRKKVFINGECIGILEFDLIDLD
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCEEEEEEEEEEEEEC
WKFHPYASYYLIEEPKIKIKPTKRKLKGKKVPVDLIENAEELKDINENDYVGVEVGNYVG
CEECCCEEEEEEECCCEEECCCHHHCCCCCCCHHHHCCHHHHHCCCCCCEEEEEECCEEE
VAVKKGDTIKIKDLTLKKELRFEKIEDYLRKNKDRIEKLEKKSLSIIKKYYEMCKNKNYA
EEEECCCEEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
INTSFSGGKDSSVSTLLANKVIDDLEVIFIDTGLEFKDTIDFVKKFAKKYDLNLVVLKGK
EECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCC
NFWEYLEKEGIPTKDYRWCNSVCKLEPLKEYLKKYKRVYTIDGSRRYESFTREKLTYERK
CHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHH
SGFIENQINIFPILDWRGTDVWSWIYLNDVIYNELYDKGFERIGCYMCPAALNAEFLRVK
CCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEECCHHCCHHHHHHH
ELYPELFNKWVDVLKRFGYDEDEILRGFWRWKELPPKMKELKKILENKEKK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDDKFASKFEIDVLNKLLNKNFSYDLAIILKKIGGLDYRKKVFINGECIGILEFDLIDLD
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCEEEEEEEEEEEEEC
WKFHPYASYYLIEEPKIKIKPTKRKLKGKKVPVDLIENAEELKDINENDYVGVEVGNYVG
CEECCCEEEEEEECCCEEECCCHHHCCCCCCCHHHHCCHHHHHCCCCCCEEEEEECCEEE
VAVKKGDTIKIKDLTLKKELRFEKIEDYLRKNKDRIEKLEKKSLSIIKKYYEMCKNKNYA
EEEECCCEEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
INTSFSGGKDSSVSTLLANKVIDDLEVIFIDTGLEFKDTIDFVKKFAKKYDLNLVVLKGK
EECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCC
NFWEYLEKEGIPTKDYRWCNSVCKLEPLKEYLKKYKRVYTIDGSRRYESFTREKLTYERK
CHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHH
SGFIENQINIFPILDWRGTDVWSWIYLNDVIYNELYDKGFERIGCYMCPAALNAEFLRVK
CCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEECCHHCCHHHHHHH
ELYPELFNKWVDVLKRFGYDEDEILRGFWRWKELPPKMKELKKILENKEKK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 5700 [C]

Specific activity: 99

Km value (mM): 0.01 {5-phosphoadenosine} [C]

Substrates: 3'-Phosphoadenylyl sulfate; Reduced thioredoxin [C]

Specific reaction: 3'-Phosphoadenylyl sulfate + Reduced thioredoxin --> (2) H+ + Adenosine 3',5'-bisphosphate + Sulfite + Oxidized thioredoxin [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: Adenosine3', 5'-bisphosphate; N-Bromosuccinimide; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087