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The map label for this gene is moaA

Identifier: 15669011

GI number: 15669011

Start: 745595

End: 746491

Strand: Reverse

Name: moaA

Synonym: MJ0824

Alternate gene names: 15669011

Gene position: 746491-745595 (Counterclockwise)

Preceding gene: 15669012

Following gene: 15669009

Centisome position: 44.84

GC content: 29.65

Gene sequence:

>897_bases
ATGAAAGATAAATTTGGTAGGGAAATTAGGTCTCTTAGAATTTCTATAACAAATAAATGCAATTTACAGTGCTTTTATTG
CCATAGAGAGGGGCATGATTCAAATAACGATAGATATATGACTCCAGAAGAAATTGGGATTATAGCAAAGACATCAACAA
AATTTGGAGTTAAAAAAATAAAAATCTCTGGTGGGGAGCCATTACTGAGGAAAGATGTTTGTGAAATTATTGAAAATATC
AAAGATGAAAGAATAAAAGACATTTCTTTAACAACCAATGGAATCCTTTTAGAAAATTTAGCTGAAAAACTTAAAGATGC
TGGGCTAAATAGAGTTAATGTGAGCTTAGACACATTAAATCCCGAATTATATAAAAAAATTACAAAATTTGGAGATGTTG
AGAGAGTAATAAATGGGATAAAGAAAGCAATAGATGTTAGCTTAACCCCTTTAAAGGTCAATTTTTTAGCAATGAGTATA
AATATTAAAGATTTACCAGATATTATGGAATTTTGTAGGGATATTGGGGCTATTTTACAAATTATTGAATTCATCCCTTT
GAAAGAAGAGCTTAAGGGCTATTATTATAACATCTCTCCAATAGAAAATGAAATTAAAGAAAAAGCTGATAAAGTTATTA
CAAGAAACTTCATGCAGAATAGGAAAAAATATATCGTTGATGGATTGGAAATAGAGTTCGTAAGGCCTATGGATAATAGT
GAGTTTTGCATGCACTGCACAAGGATAAGATTAACTTATGATGGCTATTTAAAACCATGTTTGTTGAGGGATGATAACTT
AGTTGATGTATTAACTCCATTAAGAAAAGGAGAGAATTTAGAACCATATTTTATTGAATGTATAAATAGAAGAGAGCCAT
ACTTCAAGATTAAGTAG

Upstream 100 bases:

>100_bases
ATTTATATACTTTAATTAATCTAAAATCATTACGTAGCTTTTTTATAATTAATTTCCCAATTCTATTTTTAAAACCTTAA
TTTTTTAGTAGTGAGTAAGT

Downstream 100 bases:

>100_bases
TATTTTTTAATTTTATGATATAGTTGAATATTTTTTCAATCTCTTTTGCAGCTTTTGAATCTAAGTTAATAGGTTTTCCT
AAGAATTCACTTTTTATAAC

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKDKFGREIRSLRISITNKCNLQCFYCHREGHDSNNDRYMTPEEIGIIAKTSTKFGVKKIKISGGEPLLRKDVCEIIENI
KDERIKDISLTTNGILLENLAEKLKDAGLNRVNVSLDTLNPELYKKITKFGDVERVINGIKKAIDVSLTPLKVNFLAMSI
NIKDLPDIMEFCRDIGAILQIIEFIPLKEELKGYYYNISPIENEIKEKADKVITRNFMQNRKKYIVDGLEIEFVRPMDNS
EFCMHCTRIRLTYDGYLKPCLLRDDNLVDVLTPLRKGENLEPYFIECINRREPYFKIK

Sequences:

>Translated_298_residues
MKDKFGREIRSLRISITNKCNLQCFYCHREGHDSNNDRYMTPEEIGIIAKTSTKFGVKKIKISGGEPLLRKDVCEIIENI
KDERIKDISLTTNGILLENLAEKLKDAGLNRVNVSLDTLNPELYKKITKFGDVERVINGIKKAIDVSLTPLKVNFLAMSI
NIKDLPDIMEFCRDIGAILQIIEFIPLKEELKGYYYNISPIENEIKEKADKVITRNFMQNRKKYIVDGLEIEFVRPMDNS
EFCMHCTRIRLTYDGYLKPCLLRDDNLVDVLTPLRKGENLEPYFIECINRREPYFKIK
>Mature_298_residues
MKDKFGREIRSLRISITNKCNLQCFYCHREGHDSNNDRYMTPEEIGIIAKTSTKFGVKKIKISGGEPLLRKDVCEIIENI
KDERIKDISLTTNGILLENLAEKLKDAGLNRVNVSLDTLNPELYKKITKFGDVERVINGIKKAIDVSLTPLKVNFLAMSI
NIKDLPDIMEFCRDIGAILQIIEFIPLKEELKGYYYNISPIENEIKEKADKVITRNFMQNRKKYIVDGLEIEFVRPMDNS
EFCMHCTRIRLTYDGYLKPCLLRDDNLVDVLTPLRKGENLEPYFIECINRREPYFKIK

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=294, Percent_Identity=34.3537414965986, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI22027536, Length=294, Percent_Identity=34.3537414965986, Blast_Score=145, Evalue=3e-35,
Organism=Escherichia coli, GI1786999, Length=299, Percent_Identity=28.4280936454849, Blast_Score=123, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32566331, Length=273, Percent_Identity=32.6007326007326, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32566329, Length=273, Percent_Identity=32.6007326007326, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17561210, Length=270, Percent_Identity=33.7037037037037, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI28574490, Length=274, Percent_Identity=35.7664233576642, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI28574488, Length=274, Percent_Identity=35.7664233576642, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_METJA (Q58234)

Other databases:

- EMBL:   L77117
- PIR:   H64402
- RefSeq:   NP_247815.1
- ProteinModelPortal:   Q58234
- SMR:   Q58234
- GeneID:   1451707
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0824
- NMPDR:   fig|243232.1.peg.852
- TIGR:   MJ0824
- HOGENOM:   HBG529885
- OMA:   EMENSQF
- ProtClustDB:   PRK00164
- BioCyc:   MJAN243232:MJ_0824-MONOMER
- HAMAP:   MF_01225_A
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013485
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02668

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 34653; Mature: 34653

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS01305 MOAA_NIFB_PQQE; PS00018 EF_HAND_1

Important sites: BINDING 13-13 BINDING 26-26 BINDING 61-61 BINDING 65-65 BINDING 91-91 BINDING 115-115 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKFGREIRSLRISITNKCNLQCFYCHREGHDSNNDRYMTPEEIGIIAKTSTKFGVKKI
CCCHHHHHHHHEEEEECCCCCEEEEEEECCCCCCCCCCEECHHHCEEEEECCCCCCEEEE
KISGGEPLLRKDVCEIIENIKDERIKDISLTTNGILLENLAEKLKDAGLNRVNVSLDTLN
EECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCC
PELYKKITKFGDVERVINGIKKAIDVSLTPLKVNFLAMSINIKDLPDIMEFCRDIGAILQ
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHH
IIEFIPLKEELKGYYYNISPIENEIKEKADKVITRNFMQNRKKYIVDGLEIEFVRPMDNS
HHHHCCCHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHEECCCEEEEECCCCCC
EFCMHCTRIRLTYDGYLKPCLLRDDNLVDVLTPLRKGENLEPYFIECINRREPYFKIK
HHHEEEEEEEEEECCCCCCEEECCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCEEECC
>Mature Secondary Structure
MKDKFGREIRSLRISITNKCNLQCFYCHREGHDSNNDRYMTPEEIGIIAKTSTKFGVKKI
CCCHHHHHHHHEEEEECCCCCEEEEEEECCCCCCCCCCEECHHHCEEEEECCCCCCEEEE
KISGGEPLLRKDVCEIIENIKDERIKDISLTTNGILLENLAEKLKDAGLNRVNVSLDTLN
EECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCC
PELYKKITKFGDVERVINGIKKAIDVSLTPLKVNFLAMSINIKDLPDIMEFCRDIGAILQ
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHH
IIEFIPLKEELKGYYYNISPIENEIKEKADKVITRNFMQNRKKYIVDGLEIEFVRPMDNS
HHHHCCCHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHEECCCEEEEECCCCCC
EFCMHCTRIRLTYDGYLKPCLLRDDNLVDVLTPLRKGENLEPYFIECINRREPYFKIK
HHHEEEEEEEEEECCCCCCEEECCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087