| Definition | Methanocaldococcus jannaschii DSM 2661 chromosome, complete genome. |
|---|---|
| Accession | NC_000909 |
| Length | 1,664,970 |
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The map label for this gene is bioB [C]
Identifier: 15668967
GI number: 15668967
Start: 708313
End: 709440
Strand: Direct
Name: bioB [C]
Synonym: MJ0785
Alternate gene names: 15668967
Gene position: 708313-709440 (Clockwise)
Preceding gene: 15668966
Following gene: 15668968
Centisome position: 42.54
GC content: 32.18
Gene sequence:
>1128_bases GTGAAATATACTTATAAAATAAGTAAAGTAAAAATATCTGGTGATTTAATGGTATTTGGAAAAATTGAAGAAGAGTTTAA AGAATTTTTAGAAAGTAGAAGAAAATATAACGAATTTATAAAAAATGGGCTTATAGATGAAGATGAGGCATTAAAATTAT TTAAAATAGACAATTGGAGAGATTATTTAAAGCTATTTGACATTGCCTCAAGGGTTAGGGATTATTTTAAGAAGAAGATT GAGATCACATCAACAATTCACATAACCAACATCTGCCATGTGAATCCTAAATGCTTATATTGCGGTTTTGCTGCCGGAAC TTCAAAAGAGGGCTATTATGAGCCGTTTAGATTAACAGATGAGGAGATAAAAAAATCAGCAATAGCCATTGAAGAGAGTG GGATAAAAAGAGTTAGTTGCTCTTCTGCACATGGTTATCAAGGAAAAGAAGTAATAAGAGCTTTAAAGATAGTTAAAAAA TACACAAACTTGGAAGTTTTAGTTAATGCAGGGGCTGATTTAACAGAGGAATCTATTAAAGAACTTAAAAAATATGGAAT TGATACAATATGTTGTAATTTAGAAACAATAAATGAGAATCTTTTTAAAAAGGTTAAGCCGGGAGAGGAGTTGGAAGATA GAATAAGGGTTTGTAAATTAGTTAATAAATATGATATTGAGTTATCTACTGGCTTATTGATTGGTATTGGGGAGAGTTAT GAGGATAGAGTAGAGCATTTATTTTATTTAAAAAATGAGTTAAATGTTGGGGAAATTCCAATAATGGGTTTCAACCCTTA CAAAGGAACTCCTATGGAGAATCATCCAAAATGTTCTGCTTTGGAGCAGGCAAAGACGATAGCTATAACAAGGCTGATAT TTCCAAATATAAGGATTACATCACCAACACCAACTATAGGGGCTGAGTTGGTGCAGTTTGCATTATTTGGAGGGGCGAGT AATGTAGCAACGGTTATTCCTAAAAATCATCCAATGAACGTAAAGGGAGTTGGAAATCCAAAAACTGGGAATTTGGAGGA AGTTGTTAAGATGATTATGGATTTGGGATTAAAGCCAAAGTTGGATTGGAGAAGGTATGAGAATTATTTGAAGATTTATG GAAAATAA
Upstream 100 bases:
>100_bases AAAATTGTTTCTTTTTTTGCAAAAAAATACATTAAAAATATTAAAGTCAGAAAATATTAAAAATTATTATTAATATTAAT GCAAAATAAAATATAAAAGT
Downstream 100 bases:
>100_bases TAAGTGTGGAACTATGAGGGGAGAAAGTGTTTATTTAGCCTATCCATTTATCTTAGGTAGTGTAATATTTGTTGAATTTT TTATTTTTGGCTTAGTGTAT
Product: biotin synthase
Products: biotin
Alternate protein names: NA
Number of amino acids: Translated: 375; Mature: 375
Protein sequence:
>375_residues MKYTYKISKVKISGDLMVFGKIEEEFKEFLESRRKYNEFIKNGLIDEDEALKLFKIDNWRDYLKLFDIASRVRDYFKKKI EITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTDEEIKKSAIAIEESGIKRVSCSSAHGYQGKEVIRALKIVKK YTNLEVLVNAGADLTEESIKELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGIGESY EDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRITSPTPTIGAELVQFALFGGAS NVATVIPKNHPMNVKGVGNPKTGNLEEVVKMIMDLGLKPKLDWRRYENYLKIYGK
Sequences:
>Translated_375_residues MKYTYKISKVKISGDLMVFGKIEEEFKEFLESRRKYNEFIKNGLIDEDEALKLFKIDNWRDYLKLFDIASRVRDYFKKKI EITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTDEEIKKSAIAIEESGIKRVSCSSAHGYQGKEVIRALKIVKK YTNLEVLVNAGADLTEESIKELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGIGESY EDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRITSPTPTIGAELVQFALFGGAS NVATVIPKNHPMNVKGVGNPKTGNLEEVVKMIMDLGLKPKLDWRRYENYLKIYGK >Mature_375_residues MKYTYKISKVKISGDLMVFGKIEEEFKEFLESRRKYNEFIKNGLIDEDEALKLFKIDNWRDYLKLFDIASRVRDYFKKKI EITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTDEEIKKSAIAIEESGIKRVSCSSAHGYQGKEVIRALKIVKK YTNLEVLVNAGADLTEESIKELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGIGESY EDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRITSPTPTIGAELVQFALFGGAS NVATVIPKNHPMNVKGVGNPKTGNLEEVVKMIMDLGLKPKLDWRRYENYLKIYGK
Specific function: Biotin biosynthesis; last step. [C]
COG id: COG0502
COG function: function code H; Biotin synthase and related enzymes
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: NA
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): Y785_METJA (Q58195)
Other databases:
- EMBL: L77117 - PIR: A64398 - RefSeq: NP_247771.1 - ProteinModelPortal: Q58195 - GeneID: 1451663 - GenomeReviews: L77117_GR - KEGG: mja:MJ_0785 - NMPDR: fig|243232.1.peg.808 - TIGR: MJ0785 - HOGENOM: HBG539119 - OMA: FPNIRIT - ProtClustDB: PRK06256 - BioCyc: MJAN243232:MJ_0785-MONOMER - InterPro: IPR013785 - InterPro: IPR010722 - InterPro: IPR006638 - InterPro: IPR007197 - Gene3D: G3DSA:3.20.20.70 - SMART: SM00876 - SMART: SM00729
Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM
EC number: 2.8.1.6
Molecular weight: Translated: 42810; Mature: 42810
Theoretical pI: Translated: 8.64; Mature: 8.64
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.1 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 2.1 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 4.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKYTYKISKVKISGDLMVFGKIEEEFKEFLESRRKYNEFIKNGLIDEDEALKLFKIDNWR CCEEEEEEEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHH DYLKLFDIASRVRDYFKKKIEITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTD HHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCH EEIKKSAIAIEESGIKRVSCSSAHGYQGKEVIRALKIVKKYTNLEVLVNAGADLTEESIK HHHHHHHHEEHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHH ELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGIGESY HHHHCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEECCEEEEECCCH EDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRIT HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEC SPTPTIGAELVQFALFGGASNVATVIPKNHPMNVKGVGNPKTGNLEEVVKMIMDLGLKPK CCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCC LDWRRYENYLKIYGK CCHHHHHCHHEEECC >Mature Secondary Structure MKYTYKISKVKISGDLMVFGKIEEEFKEFLESRRKYNEFIKNGLIDEDEALKLFKIDNWR CCEEEEEEEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHH DYLKLFDIASRVRDYFKKKIEITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTD HHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCH EEIKKSAIAIEESGIKRVSCSSAHGYQGKEVIRALKIVKKYTNLEVLVNAGADLTEESIK HHHHHHHHEEHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHH ELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGIGESY HHHHCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEECCEEEEECCCH EDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRIT HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEC SPTPTIGAELVQFALFGGASNVATVIPKNHPMNVKGVGNPKTGNLEEVVKMIMDLGLKPK CCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCC LDWRRYENYLKIYGK CCHHHHHCHHEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: Fe2+ [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.002 {dethiobiotin}} [C]
Substrates: dethiobiotin; sulfur
Specific reaction: dethiobiotin + sulfur = biotin
General reaction: Sulfur atom transfer [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8688087