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The map label for this gene is tfb

Identifier: 15668963

GI number: 15668963

Start: 703765

End: 705786

Strand: Reverse

Name: tfb

Synonym: MJ0782

Alternate gene names: 15668963

Gene position: 705786-703765 (Counterclockwise)

Preceding gene: 15668964

Following gene: 15668962

Centisome position: 42.39

GC content: 32.49

Gene sequence:

>2022_bases
ATGGTGTGGCTTATGGAGGCTCTCAAAACCAAAGAAAATGAAACAACAAAAGAAAAAAAACTCACAACAAAAGTTGAAAA
ATCTGAAAAAAAAGAAGAAAATGTTAGAGAGGAAGAGATTGTTTGTCCAATTTGTGGTAGTAAAGAAGTTGTTAAAGATT
ATGAAAGGGCTGAAATAGTCTGTGCTAAATGTGGATGTGTTATCAAAGAAAAATTATTTGATATTGGACCAGAATGGAGG
GCATTTGACCATGAGCAAAAGATTAAAAGATGTAGAGTTGGAGCTCCTATGACTTATAGTGTTGATTACAACGAACCAAT
AATCATTAAAGAGAATGGAGAAATAAAAGTTGTTAAAATTGGAGAACTTATAGATAAAATTATTGAAAACTCAGAGAATA
TTAGAAGAGAGGGCATCTTAGAGATAGCAAAATGTAAAGGTATTGAAGTTATTGCCTTTAACAGCAATTACAAATTTAAA
TTCATGCCTGTTTCGGAGGTTTCAAGGCATCCAGTTAGTGAGATGTTTGAAATAGTTGTTGAAGGGAATAAAAAGGTTAG
AGTTACCAGAAGCCATAGTGTCTTTACCATAAGAGATAATGAGGTAGTTCCAATAAGAGTTGATGAGCTAAAAGTTGGAG
ATATATTAGTTTTAGCAAAAGAATTGCCGAATATTGAAGAAGATATTGAAATAGATAAAAAATTTAGTAAAATATTGGGT
TACATAATTGCGGAAGGTTATTATGATGACAAAAAAATTGTATTATCTTATGATTACAATGAAAAAGAGTTTATAAATGA
AACAATTGATTATTTCAAATCTTTGAATTCGGATATAACCATCTATAGTAAAGATTTAAATATTCAAATTGAAGTAAAGA
ATAAAAAAATTATCAATTTACTAAAAAAATTGAGAGTTAAGAATAAAAGAATTCCCTCTATAATCTTTAAATCTCCTTAT
GAAATAAAAAAATCATTCATAGATGGGATATTTAATGGTAAAGATGCAAAAGTATTTGTCTCAAAGGAGTTGGCTGAAGA
TGTTATATTCTTACTTTTACAAATAAAAGAAAACGCCACCATTAATAAAAAGAGTATAAATGATATTGAAGTTTATGAGG
TAAGGAGAATAACAAATATATATACCAATAGAAAACTCGAAAAACTTATAAACTCTGATTTCATATTCTTAAAAATTAAA
GAGATTAATAAGGTAGAGCCAACCAGTGGATATGCCTATGATTTAACTGTTCCAAATGCAGAAAACTTCGTTGCTGGATT
TGGAGGATTTGTATTACACAACACCATCCACGATAAAGGTTTATCAACAGTTATTGATTGGAGAAACAAAGATAGTTATG
GAAAGGATTTATCTGCAAATAAGAGAGCCCAACTCTACAGATTAAGAAAATGGCAGAGGAGAATTAGAGTCAGTGATGCT
GCAGAGAGAAACTTAGCATTTGCCCTGTCAGAATTAGATAGAATTACATCAAAGCTCGGACTACCAAGACATGTAAGAGA
GAATGCCGCTATAATTTATAGAGGGGCTGTTGAGAAAGGATTAATAAGAGGAAGAAGTATTGAAGGAGTTGTTGCAGCCG
CTATATACGCTGCTTGCAGAAGATGTAGAGTTCCAAGAACTTTAGATGAAATTGCCGAAGCATCAAGGGTGGATAGGAAA
GAAATTGGAAGAACTTACAGATTTTTAGCGAGAGAATTAAATATAAAATTAACCCCAACAAATCCAATTGATTATGTGCC
AAGATTTGCATCTGAACTTGGATTGCCTGGGGAAGTTGAGTCCAAAGCTATACAGATATTGCAACAAGCGGCTGAAAAAG
GATTAACAAGCGGTAGAGGCCCTACTGGTGTTGCTGCTGCAGCAATATATATAGCAAGCGTTCTTCTTGGCTGTAGAAGA
ACTCAGAGGGAAGTTGCTGAAGTTGCTGGAGTGACAGAAGTAACAATAAGAAATAGATACAAGGAACTAACCGAGCATTT
GGATATTGATGTAACTCTGTAG

Upstream 100 bases:

>100_bases
AATACTCCCTATTAACAAAGATATAGAAGTTTCTGGAACTGCATATATAAAAAACGATAAATCTAAATATAAAAATACTG
AGAAGAAAAATTAAATTTAA

Downstream 100 bases:

>100_bases
ATATTATAAATAGTTAGCTAACTTTTTGTGTAGTTAAACCTTGATAATTAAAAATCAGTTAATTTTTGTTAATTTTTACG
TAATATTAATAATCTGGTGG

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB; Mja TFIIB intein; Mja Tfb intein

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MVWLMEALKTKENETTKEKKLTTKVEKSEKKEENVREEEIVCPICGSKEVVKDYERAEIVCAKCGCVIKEKLFDIGPEWR
AFDHEQKIKRCRVGAPMTYSVDYNEPIIIKENGEIKVVKIGELIDKIIENSENIRREGILEIAKCKGIEVIAFNSNYKFK
FMPVSEVSRHPVSEMFEIVVEGNKKVRVTRSHSVFTIRDNEVVPIRVDELKVGDILVLAKELPNIEEDIEIDKKFSKILG
YIIAEGYYDDKKIVLSYDYNEKEFINETIDYFKSLNSDITIYSKDLNIQIEVKNKKIINLLKKLRVKNKRIPSIIFKSPY
EIKKSFIDGIFNGKDAKVFVSKELAEDVIFLLLQIKENATINKKSINDIEVYEVRRITNIYTNRKLEKLINSDFIFLKIK
EINKVEPTSGYAYDLTVPNAENFVAGFGGFVLHNTIHDKGLSTVIDWRNKDSYGKDLSANKRAQLYRLRKWQRRIRVSDA
AERNLAFALSELDRITSKLGLPRHVRENAAIIYRGAVEKGLIRGRSIEGVVAAAIYAACRRCRVPRTLDEIAEASRVDRK
EIGRTYRFLARELNIKLTPTNPIDYVPRFASELGLPGEVESKAIQILQQAAEKGLTSGRGPTGVAAAAIYIASVLLGCRR
TQREVAEVAGVTEVTIRNRYKELTEHLDIDVTL

Sequences:

>Translated_673_residues
MVWLMEALKTKENETTKEKKLTTKVEKSEKKEENVREEEIVCPICGSKEVVKDYERAEIVCAKCGCVIKEKLFDIGPEWR
AFDHEQKIKRCRVGAPMTYSVDYNEPIIIKENGEIKVVKIGELIDKIIENSENIRREGILEIAKCKGIEVIAFNSNYKFK
FMPVSEVSRHPVSEMFEIVVEGNKKVRVTRSHSVFTIRDNEVVPIRVDELKVGDILVLAKELPNIEEDIEIDKKFSKILG
YIIAEGYYDDKKIVLSYDYNEKEFINETIDYFKSLNSDITIYSKDLNIQIEVKNKKIINLLKKLRVKNKRIPSIIFKSPY
EIKKSFIDGIFNGKDAKVFVSKELAEDVIFLLLQIKENATINKKSINDIEVYEVRRITNIYTNRKLEKLINSDFIFLKIK
EINKVEPTSGYAYDLTVPNAENFVAGFGGFVLHNTIHDKGLSTVIDWRNKDSYGKDLSANKRAQLYRLRKWQRRIRVSDA
AERNLAFALSELDRITSKLGLPRHVRENAAIIYRGAVEKGLIRGRSIEGVVAAAIYAACRRCRVPRTLDEIAEASRVDRK
EIGRTYRFLARELNIKLTPTNPIDYVPRFASELGLPGEVESKAIQILQQAAEKGLTSGRGPTGVAAAAIYIASVLLGCRR
TQREVAEVAGVTEVTIRNRYKELTEHLDIDVTL
>Mature_673_residues
MVWLMEALKTKENETTKEKKLTTKVEKSEKKEENVREEEIVCPICGSKEVVKDYERAEIVCAKCGCVIKEKLFDIGPEWR
AFDHEQKIKRCRVGAPMTYSVDYNEPIIIKENGEIKVVKIGELIDKIIENSENIRREGILEIAKCKGIEVIAFNSNYKFK
FMPVSEVSRHPVSEMFEIVVEGNKKVRVTRSHSVFTIRDNEVVPIRVDELKVGDILVLAKELPNIEEDIEIDKKFSKILG
YIIAEGYYDDKKIVLSYDYNEKEFINETIDYFKSLNSDITIYSKDLNIQIEVKNKKIINLLKKLRVKNKRIPSIIFKSPY
EIKKSFIDGIFNGKDAKVFVSKELAEDVIFLLLQIKENATINKKSINDIEVYEVRRITNIYTNRKLEKLINSDFIFLKIK
EINKVEPTSGYAYDLTVPNAENFVAGFGGFVLHNTIHDKGLSTVIDWRNKDSYGKDLSANKRAQLYRLRKWQRRIRVSDA
AERNLAFALSELDRITSKLGLPRHVRENAAIIYRGAVEKGLIRGRSIEGVVAAAIYAACRRCRVPRTLDEIAEASRVDRK
EIGRTYRFLARELNIKLTPTNPIDYVPRFASELGLPGEVESKAIQILQQAAEKGLTSGRGPTGVAAAAIYIASVLLGCRR
TQREVAEVAGVTEVTIRNRYKELTEHLDIDVTL

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: COG1372

COG function: function code L; Intein/homing endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger

Homologues:

Organism=Homo sapiens, GI4504193, Length=192, Percent_Identity=35.9375, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI22035556, Length=187, Percent_Identity=33.1550802139037, Blast_Score=97, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17564892, Length=182, Percent_Identity=36.8131868131868, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531397, Length=185, Percent_Identity=29.1891891891892, Blast_Score=89, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6325343, Length=213, Percent_Identity=29.5774647887324, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321685, Length=186, Percent_Identity=27.4193548387097, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17136758, Length=197, Percent_Identity=34.5177664974619, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281361961, Length=173, Percent_Identity=32.3699421965318, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24647658, Length=173, Percent_Identity=32.3699421965318, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B_METJA (Q58192)

Other databases:

- EMBL:   L77117
- PIR:   F64397
- RefSeq:   NP_247767.1
- ProteinModelPortal:   Q58192
- GeneID:   1451659
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0782
- NMPDR:   fig|243232.1.peg.804
- TIGR:   MJ0782
- OMA:   SEMFEIV
- ProtClustDB:   CLSK2517657
- BioCyc:   MJAN243232:MJ_0782-MONOMER
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00379
- PRINTS:   PR00685
- SMART:   SM00385
- SMART:   SM00305
- SMART:   SM00306
- TIGRFAMs:   TIGR01443
- TIGRFAMs:   TIGR01445

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 76970; Mature: 76970

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS50818 INTEIN_C_TER; PS50819 INTEIN_ENDONUCLEASE; PS50817 INTEIN_N_TER; PS00782 TFIIB; PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWLMEALKTKENETTKEKKLTTKVEKSEKKEENVREEEIVCPICGSKEVVKDYERAEIV
CEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCHHHHHHHHHHHEE
CAKCGCVIKEKLFDIGPEWRAFDHEQKIKRCRVGAPMTYSVDYNEPIIIKENGEIKVVKI
HHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCEEEECCCCEEEEEH
GELIDKIIENSENIRREGILEIAKCKGIEVIAFNSNYKFKFMPVSEVSRHPVSEMFEIVV
HHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEHHHHHCCCHHHHHHHHH
EGNKKVRVTRSHSVFTIRDNEVVPIRVDELKVGDILVLAKELPNIEEDIEIDKKFSKILG
CCCCEEEEEECCEEEEEECCCEEEEEECCEECCCEEEEHHHCCCCHHHHHHHHHHHHHHH
YIIAEGYYDDKKIVLSYDYNEKEFINETIDYFKSLNSDITIYSKDLNIQIEVKNKKIINL
HHHHCCEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECEEEEEEECCHHHHHH
LKKLRVKNKRIPSIIFKSPYEIKKSFIDGIFNGKDAKVFVSKELAEDVIFLLLQIKENAT
HHHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCE
INKKSINDIEVYEVRRITNIYTNRKLEKLINSDFIFLKIKEINKVEPTSGYAYDLTVPNA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEEECCCC
ENFVAGFGGFVLHNTIHDKGLSTVIDWRNKDSYGKDLSANKRAQLYRLRKWQRRIRVSDA
HHHHHHCCHHHHCCCHHHCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCH
AERNLAFALSELDRITSKLGLPRHVRENAAIIYRGAVEKGLIRGRSIEGVVAAAIYAACR
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCHHHHHHCCCCCCHHHHHHHHHHHH
RCRVPRTLDEIAEASRVDRKEIGRTYRFLARELNIKLTPTNPIDYVPRFASELGLPGEVE
HCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCCHH
SKAIQILQQAAEKGLTSGRGPTGVAAAAIYIASVLLGCRRTQREVAEVAGVTEVTIRNRY
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KELTEHLDIDVTL
HHHHHHCCCEEEC
>Mature Secondary Structure
MVWLMEALKTKENETTKEKKLTTKVEKSEKKEENVREEEIVCPICGSKEVVKDYERAEIV
CEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCHHHHHHHHHHHEE
CAKCGCVIKEKLFDIGPEWRAFDHEQKIKRCRVGAPMTYSVDYNEPIIIKENGEIKVVKI
HHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCEEEECCCCEEEEEH
GELIDKIIENSENIRREGILEIAKCKGIEVIAFNSNYKFKFMPVSEVSRHPVSEMFEIVV
HHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEHHHHHCCCHHHHHHHHH
EGNKKVRVTRSHSVFTIRDNEVVPIRVDELKVGDILVLAKELPNIEEDIEIDKKFSKILG
CCCCEEEEEECCEEEEEECCCEEEEEECCEECCCEEEEHHHCCCCHHHHHHHHHHHHHHH
YIIAEGYYDDKKIVLSYDYNEKEFINETIDYFKSLNSDITIYSKDLNIQIEVKNKKIINL
HHHHCCEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECEEEEEEECCHHHHHH
LKKLRVKNKRIPSIIFKSPYEIKKSFIDGIFNGKDAKVFVSKELAEDVIFLLLQIKENAT
HHHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCE
INKKSINDIEVYEVRRITNIYTNRKLEKLINSDFIFLKIKEINKVEPTSGYAYDLTVPNA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEEECCCC
ENFVAGFGGFVLHNTIHDKGLSTVIDWRNKDSYGKDLSANKRAQLYRLRKWQRRIRVSDA
HHHHHHCCHHHHCCCHHHCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCH
AERNLAFALSELDRITSKLGLPRHVRENAAIIYRGAVEKGLIRGRSIEGVVAAAIYAACR
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCHHHHHHCCCCCCHHHHHHHHHHHH
RCRVPRTLDEIAEASRVDRKEIGRTYRFLARELNIKLTPTNPIDYVPRFASELGLPGEVE
HCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCCHH
SKAIQILQQAAEKGLTSGRGPTGVAAAAIYIASVLLGCRRTQREVAEVAGVTEVTIRNRY
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KELTEHLDIDVTL
HHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087