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The map label for this gene is aksA [H]

Identifier: 15668680

GI number: 15668680

Start: 444533

End: 445753

Strand: Direct

Name: aksA [H]

Synonym: MJ0503

Alternate gene names: 15668680

Gene position: 444533-445753 (Clockwise)

Preceding gene: 15668679

Following gene: 15668681

Centisome position: 26.7

GC content: 37.26

Gene sequence:

>1221_bases
ATGACAAAAGTGCTGGTGATGTTTATGGATTTCTTATTTGAGAACAGCTGGAAAGCAGTTTGTCCCTACAATCCAAAGTT
GGATTTAAAGGACATTTATATTTATGACACAACCCTAAGAGATGGAGAGCAAACCCCAGGAGTTTGCTTTACCAAAGAAC
AAAAATTGGAGATTGCAAGGAAGTTGGATGAACTTGGATTAAAGCAGATTGAAGCTGGCTTCCCAATAGTATCTGAAAGA
GAAGCAGATATAGTTAAAACAATTGCTAATGAAGGGCTAAATGCTGATATCTTAGCTTTATGCAGGGCTTTAAAGAAAGA
TATAGATAAAGCAATAGAGTGCGATGTAGATGGGATTATTACCTTCATAGCAACATCTCCTCTCCACTTAAAATATAAAT
TCAACAACAAAAGCTTAGATGAAATATTAGAGATGGGAGTTGAGGCAGTTGAGTATGCAAAGGAACATGGCTTATTTGTT
GCTTTCTCTGCAGAGGATGCGACAAGAACACCAATAGAGGACTTGATTAAAGTGCATAAAGCCGCTGAAGAGGCTGGAGC
AGATAGGGTTCATATAGCAGACACAACTGGCTGTGCTACCCCCCAAAGTATGGAGTTTATATGTAAAACATTGAAGGAGA
ACTTAAAAAAGGCACATATTGGAGTGCATTGTCACAACGACTTTGGATTTGCAGTTATAAATTCAATATATGGTTTAATT
GGAGGAGCTAAGGCAGTTTCAACAACAGTTAATGGTATTGGAGAGAGGGCAGGGAATGCAGCTTTAGAAGAGCTAATTAT
GGCTTTAACTGTCTTGTATGATGTTGATTTGGGATTAAACTTGGAGGTTCTTCCAGAGTTATGCAGAATGGTTGAGGAAT
ACTCTGGAATAAAGATGCCAAAGAACAAACCAATAGTTGGAGAGCTTGTATTTGCTCATGAAAGTGGAATTCACGTTGAT
GCTGTCATAGAGAATCCATTAACCTATGAACCCTTCCTTCCAGAGAAAATAGGGCTTAAGAGAAATATTTTGTTAGGGAA
GCATTCTGGATGCAGAGCCGTTGCCTATAAGCTAAAACTTATGGGAATTGATTACGATAGAGAGATGTTGTGCGAGATTG
TTAAAAAGGTTAAAGAGATTAGAGAGGAAGGTAAATTTATAACTGATGAAGTCTTTAAGGAGATTGTTGAAGAAGTTTTA
AGGAAGAGAAATAAAAATTAA

Upstream 100 bases:

>100_bases
TATAGAGGTAAAATAAAAAGTAAGTGAATAAATCAATCTCTTCATATTGATATTTTGATTAAAAAATAATAAAAAAATTT
ATACAGGTAATAACAACTCT

Downstream 100 bases:

>100_bases
CAAATAAAATTATCATGGAGGGAAGGAATAATGAAACTCTTTGACTATCCAGGAATTCAAAATACAGATGAGACATTAAA
AATAGCTGTTGAGAGGGCTA

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1 [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTKVLVMFMDFLFENSWKAVCPYNPKLDLKDIYIYDTTLRDGEQTPGVCFTKEQKLEIARKLDELGLKQIEAGFPIVSER
EADIVKTIANEGLNADILALCRALKKDIDKAIECDVDGIITFIATSPLHLKYKFNNKSLDEILEMGVEAVEYAKEHGLFV
AFSAEDATRTPIEDLIKVHKAAEEAGADRVHIADTTGCATPQSMEFICKTLKENLKKAHIGVHCHNDFGFAVINSIYGLI
GGAKAVSTTVNGIGERAGNAALEELIMALTVLYDVDLGLNLEVLPELCRMVEEYSGIKMPKNKPIVGELVFAHESGIHVD
AVIENPLTYEPFLPEKIGLKRNILLGKHSGCRAVAYKLKLMGIDYDREMLCEIVKKVKEIREEGKFITDEVFKEIVEEVL
RKRNKN

Sequences:

>Translated_406_residues
MTKVLVMFMDFLFENSWKAVCPYNPKLDLKDIYIYDTTLRDGEQTPGVCFTKEQKLEIARKLDELGLKQIEAGFPIVSER
EADIVKTIANEGLNADILALCRALKKDIDKAIECDVDGIITFIATSPLHLKYKFNNKSLDEILEMGVEAVEYAKEHGLFV
AFSAEDATRTPIEDLIKVHKAAEEAGADRVHIADTTGCATPQSMEFICKTLKENLKKAHIGVHCHNDFGFAVINSIYGLI
GGAKAVSTTVNGIGERAGNAALEELIMALTVLYDVDLGLNLEVLPELCRMVEEYSGIKMPKNKPIVGELVFAHESGIHVD
AVIENPLTYEPFLPEKIGLKRNILLGKHSGCRAVAYKLKLMGIDYDREMLCEIVKKVKEIREEGKFITDEVFKEIVEEVL
RKRNKN
>Mature_405_residues
TKVLVMFMDFLFENSWKAVCPYNPKLDLKDIYIYDTTLRDGEQTPGVCFTKEQKLEIARKLDELGLKQIEAGFPIVSERE
ADIVKTIANEGLNADILALCRALKKDIDKAIECDVDGIITFIATSPLHLKYKFNNKSLDEILEMGVEAVEYAKEHGLFVA
FSAEDATRTPIEDLIKVHKAAEEAGADRVHIADTTGCATPQSMEFICKTLKENLKKAHIGVHCHNDFGFAVINSIYGLIG
GAKAVSTTVNGIGERAGNAALEELIMALTVLYDVDLGLNLEVLPELCRMVEEYSGIKMPKNKPIVGELVFAHESGIHVDA
VIENPLTYEPFLPEKIGLKRNILLGKHSGCRAVAYKLKLMGIDYDREMLCEIVKKVKEIREEGKFITDEVFKEIVEEVLR
KRNKN

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=344, Percent_Identity=41.5697674418605, Blast_Score=239, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6320071, Length=378, Percent_Identity=33.0687830687831, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6320019, Length=367, Percent_Identity=32.9700272479564, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324225, Length=386, Percent_Identity=26.6839378238342, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324682, Length=386, Percent_Identity=26.1658031088083, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24582381, Length=284, Percent_Identity=25.7042253521127, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 45365; Mature: 45234

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVLVMFMDFLFENSWKAVCPYNPKLDLKDIYIYDTTLRDGEQTPGVCFTKEQKLEIAR
CHHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEEEEEECCCCCCCCCEEECHHHHHHHHH
KLDELGLKQIEAGFPIVSEREADIVKTIANEGLNADILALCRALKKDIDKAIECDVDGII
HHHHHCHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
TFIATSPLHLKYKFNNKSLDEILEMGVEAVEYAKEHGLFVAFSAEDATRTPIEDLIKVHK
HEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHH
AAEEAGADRVHIADTTGCATPQSMEFICKTLKENLKKAHIGVHCHNDFGFAVINSIYGLI
HHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHH
GGAKAVSTTVNGIGERAGNAALEELIMALTVLYDVDLGLNLEVLPELCRMVEEYSGIKMP
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
KNKPIVGELVFAHESGIHVDAVIENPLTYEPFLPEKIGLKRNILLGKHSGCRAVAYKLKL
CCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCHHHCCCCCEEEECCCCHHHHEEEEEE
MGIDYDREMLCEIVKKVKEIREEGKFITDEVFKEIVEEVLRKRNKN
ECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKVLVMFMDFLFENSWKAVCPYNPKLDLKDIYIYDTTLRDGEQTPGVCFTKEQKLEIAR
HHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEEEEEECCCCCCCCCEEECHHHHHHHHH
KLDELGLKQIEAGFPIVSEREADIVKTIANEGLNADILALCRALKKDIDKAIECDVDGII
HHHHHCHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
TFIATSPLHLKYKFNNKSLDEILEMGVEAVEYAKEHGLFVAFSAEDATRTPIEDLIKVHK
HEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHH
AAEEAGADRVHIADTTGCATPQSMEFICKTLKENLKKAHIGVHCHNDFGFAVINSIYGLI
HHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHH
GGAKAVSTTVNGIGERAGNAALEELIMALTVLYDVDLGLNLEVLPELCRMVEEYSGIKMP
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
KNKPIVGELVFAHESGIHVDAVIENPLTYEPFLPEKIGLKRNILLGKHSGCRAVAYKLKL
CCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCHHHCCCCCEEEECCCCHHHHEEEEEE
MGIDYDREMLCEIVKKVKEIREEGKFITDEVFKEIVEEVLRKRNKN
ECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]