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The map label for this gene is pheS

Identifier: 15668664

GI number: 15668664

Start: 430927

End: 432369

Strand: Direct

Name: pheS

Synonym: MJ0487

Alternate gene names: 15668664

Gene position: 430927-432369 (Clockwise)

Preceding gene: 15668662

Following gene: 15668665

Centisome position: 25.88

GC content: 29.87

Gene sequence:

>1443_bases
ATGGAACTACATATAGATGAAAAGAGATTGTTAAAGATTTTTCAGGATAATAATAGAGATGAGTTTAATTTAAATGAATT
AGAGAAGTTCATGCCAAAAGAAAAGATTTTAAGGGTTTCTTTATGGTTAAAAGGTAAAAATTTGGTAGAAACAGAAGAAA
AAGTAAAGAAGATAATAAAACTCATTAAAGAAGAAGAGTTTCCAGAGAGGAAAATAGCAAACTATTTAAAACAACACAAC
ATCAAAGAAATTGAAATTAAAAACTTAAAAGATATTTTACCAAAAGAGGAAATTAATGCTGCCTTAGGTGCTATAAAAAG
AAAAGGAATAGCAAGGATTGAGAAAGGTAAAATTATTTTTGATAATTTAGATTACAAGGATGTTGAAGAACAACTATTAC
AAAAAATCAAAGAAAATAAATACCTCGACGACTTTAGTGAAGAGGAAAAAAAGATTATAGACATTTTAAAAAAGAGAGGT
TATGTAGATTTTGATGAAGAGAAAGAAATAAAGATAAAATTAACTGAAAAAGGAAAAGAGTTCATAAAAAATCCAATAGA
GATTGAAGAAGAGATTACCCAATTAACAAGAGATATTATAATAAGTGGAAAGTGGAAAAAAGCATATATAAGACCTTATG
ACGTAAAAGTTCCTACCAAGCCAATATACCCAGCTAAAGTCCATCCATTGACAAGAATTATTAGAGAGGTTAAAGAGATT
TTATTAGCTATGGGATTTAAAGAAGTGAAAAGCCCAATTGTAGAAACAGAGTTTTGGAACTTTGATATGTTATTTGAACC
ACAAGACCATCCAGCAAGAGAAATGCAAGACACTTTCTTCTTAAAATATCCAAATGAGGGAGATATTCCAGAAGATTTAT
TAAGTAAGGTTAAAGAAGTTCATGAAAGATGTTGGAAATACAAATTTGATGAAAATGTCTCAAGAAGATTGATTTTAAGA
ACTCATACCACTGCATCATCAATAAGATACCTTGCATCTTTATCAGATGAAGAAAAAAACAAGCCTCACAAGGTATTTTG
TATAGATAGAGTATTTAGAAATGAAGCAATTGATTATAAACATTTGCCAGAGTTTTATCAGTGTGAAGGAATTATAATGG
ATGATAATGTTAATTTTAACAACCTAATTGGAGTTTTAAAAGAATTCTTAAATAGATTGGGCTTTGAAAAGGTTAGATTT
AGGCCAGCTTACTTCCCATTCACTGAGCCATCCTTAGAGGCAGAGGTTTATTTAGAGGGTAAAGGCTGGTTAGAAATCTT
AGGAGCAGGAATATTTAGACCAGAAGTTTTAGAGCCAATTGGTATTGAAAAGCCAGTTTTAGCTTGGGGTATTGGGTTTA
GTAGATTAGCAATGCTTAGATATGGATTGACAGACATTAGAGATTTGCATAAGAATGATTTAGATTGGTTGAAGAGAGTA
TAA

Upstream 100 bases:

>100_bases
TTGTAGATTTAAACATGGGTTGATAAACATAGTTATCTATTTATTAAAGAAAATAATACTATAAAAAGAATAAAATTTAG
CAGTTCTGTATGGTGGGATT

Downstream 100 bases:

>100_bases
ACTAAAATAAGTGATTGTAATGGAGGAAATTATTAAAAAAGCTTTTATTGAATCAATTAATAATATTAGGAGAGGGGATA
AAGAGGAAGAACTAAAAAAA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MELHIDEKRLLKIFQDNNRDEFNLNELEKFMPKEKILRVSLWLKGKNLVETEEKVKKIIKLIKEEEFPERKIANYLKQHN
IKEIEIKNLKDILPKEEINAALGAIKRKGIARIEKGKIIFDNLDYKDVEEQLLQKIKENKYLDDFSEEEKKIIDILKKRG
YVDFDEEKEIKIKLTEKGKEFIKNPIEIEEEITQLTRDIIISGKWKKAYIRPYDVKVPTKPIYPAKVHPLTRIIREVKEI
LLAMGFKEVKSPIVETEFWNFDMLFEPQDHPAREMQDTFFLKYPNEGDIPEDLLSKVKEVHERCWKYKFDENVSRRLILR
THTTASSIRYLASLSDEEKNKPHKVFCIDRVFRNEAIDYKHLPEFYQCEGIIMDDNVNFNNLIGVLKEFLNRLGFEKVRF
RPAYFPFTEPSLEAEVYLEGKGWLEILGAGIFRPEVLEPIGIEKPVLAWGIGFSRLAMLRYGLTDIRDLHKNDLDWLKRV

Sequences:

>Translated_480_residues
MELHIDEKRLLKIFQDNNRDEFNLNELEKFMPKEKILRVSLWLKGKNLVETEEKVKKIIKLIKEEEFPERKIANYLKQHN
IKEIEIKNLKDILPKEEINAALGAIKRKGIARIEKGKIIFDNLDYKDVEEQLLQKIKENKYLDDFSEEEKKIIDILKKRG
YVDFDEEKEIKIKLTEKGKEFIKNPIEIEEEITQLTRDIIISGKWKKAYIRPYDVKVPTKPIYPAKVHPLTRIIREVKEI
LLAMGFKEVKSPIVETEFWNFDMLFEPQDHPAREMQDTFFLKYPNEGDIPEDLLSKVKEVHERCWKYKFDENVSRRLILR
THTTASSIRYLASLSDEEKNKPHKVFCIDRVFRNEAIDYKHLPEFYQCEGIIMDDNVNFNNLIGVLKEFLNRLGFEKVRF
RPAYFPFTEPSLEAEVYLEGKGWLEILGAGIFRPEVLEPIGIEKPVLAWGIGFSRLAMLRYGLTDIRDLHKNDLDWLKRV
>Mature_480_residues
MELHIDEKRLLKIFQDNNRDEFNLNELEKFMPKEKILRVSLWLKGKNLVETEEKVKKIIKLIKEEEFPERKIANYLKQHN
IKEIEIKNLKDILPKEEINAALGAIKRKGIARIEKGKIIFDNLDYKDVEEQLLQKIKENKYLDDFSEEEKKIIDILKKRG
YVDFDEEKEIKIKLTEKGKEFIKNPIEIEEEITQLTRDIIISGKWKKAYIRPYDVKVPTKPIYPAKVHPLTRIIREVKEI
LLAMGFKEVKSPIVETEFWNFDMLFEPQDHPAREMQDTFFLKYPNEGDIPEDLLSKVKEVHERCWKYKFDENVSRRLILR
THTTASSIRYLASLSDEEKNKPHKVFCIDRVFRNEAIDYKHLPEFYQCEGIIMDDNVNFNNLIGVLKEFLNRLGFEKVRF
RPAYFPFTEPSLEAEVYLEGKGWLEILGAGIFRPEVLEPIGIEKPVLAWGIGFSRLAMLRYGLTDIRDLHKNDLDWLKRV

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=321, Percent_Identity=39.8753894080997, Blast_Score=251, Evalue=9e-67,
Organism=Escherichia coli, GI1788007, Length=272, Percent_Identity=38.2352941176471, Blast_Score=175, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI32563657, Length=321, Percent_Identity=40.4984423676012, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17508957, Length=321, Percent_Identity=40.4984423676012, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI32566635, Length=272, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321087, Length=365, Percent_Identity=38.9041095890411, Blast_Score=259, Evalue=5e-70,
Organism=Drosophila melanogaster, GI18858079, Length=408, Percent_Identity=35.7843137254902, Blast_Score=239, Evalue=4e-63,
Organism=Drosophila melanogaster, GI17137424, Length=100, Percent_Identity=41, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_METJA (Q57911)

Other databases:

- EMBL:   L77117
- PIR:   G64360
- RefSeq:   NP_247463.1
- ProteinModelPortal:   Q57911
- SMR:   Q57911
- GeneID:   1451349
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0487
- NMPDR:   fig|243232.1.peg.500
- TIGR:   MJ0487
- HOGENOM:   HBG497668
- OMA:   PEFYQCE
- ProtClustDB:   PRK04172
- BioCyc:   MJAN243232:MJ_0487-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00282
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF01409 tRNA-synt_2d

EC number: =6.1.1.20

Molecular weight: Translated: 57028; Mature: 57028

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELHIDEKRLLKIFQDNNRDEFNLNELEKFMPKEKILRVSLWLKGKNLVETEEKVKKIIK
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHEEEEEEECCCCCHHHHHHHHHHHH
LIKEEEFPERKIANYLKQHNIKEIEIKNLKDILPKEEINAALGAIKRKGIARIEKGKIIF
HHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCEEE
DNLDYKDVEEQLLQKIKENKYLDDFSEEEKKIIDILKKRGYVDFDEEKEIKIKLTEKGKE
ECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHH
FIKNPIEIEEEITQLTRDIIISGKWKKAYIRPYDVKVPTKPIYPAKVHPLTRIIREVKEI
HHCCCCHHHHHHHHHHHHHEECCCCCEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LLAMGFKEVKSPIVETEFWNFDMLFEPQDHPAREMQDTFFLKYPNEGDIPEDLLSKVKEV
HHHHCHHHHHCCHHCCCCCCEEEEECCCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHHH
HERCWKYKFDENVSRRLILRTHTTASSIRYLASLSDEEKNKPHKVFCIDRVFRNEAIDYK
HHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHCCCCCEEEEHHHHHHHCCCCHH
HLPEFYQCEGIIMDDNVNFNNLIGVLKEFLNRLGFEKVRFRPAYFPFTEPSLEAEVYLEG
HCCHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCEEEEECC
KGWLEILGAGIFRPEVLEPIGIEKPVLAWGIGFSRLAMLRYGLTDIRDLHKNDLDWLKRV
CCHHHHHHCCCCCCHHHCCCCCCCCHHHHCCCHHHHHHHHHCHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MELHIDEKRLLKIFQDNNRDEFNLNELEKFMPKEKILRVSLWLKGKNLVETEEKVKKIIK
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHEEEEEEECCCCCHHHHHHHHHHHH
LIKEEEFPERKIANYLKQHNIKEIEIKNLKDILPKEEINAALGAIKRKGIARIEKGKIIF
HHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCEEE
DNLDYKDVEEQLLQKIKENKYLDDFSEEEKKIIDILKKRGYVDFDEEKEIKIKLTEKGKE
ECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHH
FIKNPIEIEEEITQLTRDIIISGKWKKAYIRPYDVKVPTKPIYPAKVHPLTRIIREVKEI
HHCCCCHHHHHHHHHHHHHEECCCCCEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LLAMGFKEVKSPIVETEFWNFDMLFEPQDHPAREMQDTFFLKYPNEGDIPEDLLSKVKEV
HHHHCHHHHHCCHHCCCCCCEEEEECCCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHHH
HERCWKYKFDENVSRRLILRTHTTASSIRYLASLSDEEKNKPHKVFCIDRVFRNEAIDYK
HHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHCCCCCEEEEHHHHHHHCCCCHH
HLPEFYQCEGIIMDDNVNFNNLIGVLKEFLNRLGFEKVRFRPAYFPFTEPSLEAEVYLEG
HCCHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCEEEEECC
KGWLEILGAGIFRPEVLEPIGIEKPVLAWGIGFSRLAMLRYGLTDIRDLHKNDLDWLKRV
CCHHHHHHCCCCCCHHHCCCCCCCCHHHHCCCHHHHHHHHHCHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087